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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 邱啟銘 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:50Z | - |
| dc.date.available | 2021-07-01T08:17:50Z | - |
| dc.date.issued | 1994 | |
| dc.identifier.citation | 1. Belenguer , P., Baldin, V., Mathieu, C., Prats, H., Bensaid, M. , Bouche , G. and Amalric , F. (1989) . Protein Kinase N Ⅱ and the regulation of rDNA transcription in mammalian cells. Nucleic Acids Res .17 : 6625 - 6636 2. Belenguer , P. , Caizergues - Ferrer , M. , Labbe , J.C. , Doree, M. and Amalric , F. (1990) . Mitosis - specific phosphorylation of nucleolin by p34cdc2 protein kinase. Mol. Cell. Biol. 10 : 3607 - 3618 3. Borer , R. A. , Lehner , C.F. , Eppenberger , H.M. , Nigg , E.A. (1989) . Major nucleolar proteins shuttle between nucleus and cytoplasm. Cell 56 : 379 - 390 4. Bouche ,G. , Caizergues - Ferrer , M. , Bugler , B. and Amalric , F. (1984). Interrelations between the maturation of a 100 kDa nucleolar protein and pre rRNA synthesis in CHO cells . Nucleic Acids Res. 12 : 3025-3035 5. Caizergues - Ferrer , M. , Belenguer, P. , Lapeyre , B. ,Amalric , F. , Wallace , M.O. and Olson , M.O.J. (1987) . 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Sci. USA 90 : 7769 -7773 11. Jordan, G. (1987). At the heart of the nucleolus. Nature 329 : 489-490 12. Kharrat , A. , Derancourt , J., Doree , M. , Amalric , F. and Erard , M. (1991). Synergistic effect of histone H1 and nucleolin on chromatin condensation in mitosis : role of a phosphorylated heteromer. Biochemistry 30 : 10329-10336 13. Krek , W. , Maridor, C. and Nigg , E.A. (1992). Casein kinase Ⅱ is a predominantly nuclear enzyme. J.Cell Biol.116 : 43 -55 14. Lapeyre , B. , Bourbon , H. and Amairic , F. (1987). Nucleolin , the major nucleolar protein of growing eukaryotic cells : an unusual protein structure revealed by the nucleotide sequence. proc. Natl. Acad. Sci. USA 84 : 1472-1476 15. Laskey , R.A. and Dingwall , C. (1993) . Nuclear shuttling the default pathway for nuclear proteins Cell 74 , 585 - 586 16. Lee , Y.M. ,Tsai , W.H. , Lai , M.Y. , Chen ,D.S. and Lee , S.C. (1993). Induction of liver alpha-1 acid glycoprotein gene expression involves both positive and negative transcription factors. Mol. Cell. Biol 13 : 432 -442 17. Lin , A. , Frost , J. , Deng ,T. , Smeal , T. , Al-Alawi , N. , Kikkawa , U. , Hunter , T. , Brenner, D. and Karin , M. (1992)Casein kinase Ⅱ is a negative regulator of c-Jun DNA binding and AP-l activity. Cell 70 : 777 - 789 18. Matthews , H.R. and Huebner , V.D. (1984). Nuclear protein kinases. Mol. Cell. Biochem. 59 : 81 - 99 19. Meier , T. and Blobel , G. (1990). A nuclear localization signal binding protein in the nucleolus. J. Cell Biol.111 : 2235 -2245 20. Meier , T. and Blobel , G. (1992). Nopp140 shuttles on tracks between nucleolus and cytoplasm. Cell 70 : 127 - 138 21. Mills , J.S. , Busch , H. and Durban , E. (1982) Purification of a protein kinase from human Namalwa cells that phosphorylates topoisomerase I. Biochem. Biophys. Res. Comm. 109 : 1222 - 1227 22. Ochs , R. , Lischwe , M. , O, Leary P. and Busch, H. (1983). Localization of nucleolar phosphoproteins B23 and C23 during mitosis Exp. Cell Res. 146 : 139 - 149 23. Olson , M.O.J. , Kirstein , M.N. and Wallace , M.O. (1990). Limited proteolysis as a probe of the conformation and nucleic acid binding regions of nucleolin. Biochemistry 29 : 5682 -5686 24. Pasternack , M.S. , Bleier, K.J. and McInerney ,T.N. (1991). Granzyme A binding to target cell proteins. J. Biol. Chem 266 : 14703 - 14708 25. Peter , M. , Nakagawa , J. , Doree , M., Labbe , J.C. and Nigg , E.A. (1990). Identification of major nucleolar proteins as candidate mitotic substrates of cdc2 kinase. Cell 60 : 791- 801 26. Pinna , L.A. (1990) . Casein kinase Ⅱ: an ‘eminence grise’ in cellular regulation ? Biochem. Biophys. Acta. 1054 : 267 - 284 27. Rihs , H.P. , Jans , D.A. , Fan , H. and Peters , R. (1991). The rate of nuclear cytoplasmic protein transport is determined by the casein kinase Ⅱ site flanking the nuclear localization sequence of the SV40 T-antigen. EMBO J. 10 : 633 - 639 28. Schmidt-Zachmann , M.S. , Dargemont , C. ,Kuhn , L.C. and Nigg ,E.A. (1993). Nuclear export of proteins : the role of nuclear retention. Cell 74 : 493 - 504 29. Suzuki , N. ,Matsui ,H. and Hosoya ,T. (1985). Effects of androgen and polyamines on the phosphorylation of nuciear proteins from rat ventral prostates with particular reference to 110-kDa phosphoprotein. J. Biol. Chem. 260 : 8050- 8055 30. Suzuki , T. , Suzuki ,N. and Hosoya ,T. (1993). Limited proteolysis of rat liver nucleolin by endogeneous proteases : effects of polyamines and histones. Biochem. J. 289 : 109 - 115 31. Warrener , P. and Petrysnyn , R. (1991). Phosphorylation and proteolytic degradation of nucleolin from 3T3-F442A cells. Biochem. Biophys. Res. Commun. 180 : 716 - 723 32. Yang , T.H. ,Tsai ,W.H. , Lee , Y.M. , Lei , H.Y. , Lai , M.Y. Chen , D.S. , Yeh , N.H. and Lee, S.C. Purification ,characterization, and identification of nucleolin as a transcription repressor. Mol. Cell. Biol. in press. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76078 | - |
| dc.description.abstract | 從C3H小老鼠，肝癌細胞株（129P）之核萃取液，純化可結合於AGP?動子中負向調控區域之蛋白質，找出 nucleolin為可能之因數。而nucleolin為核仁之主要蛋白質，其生物功能包括核糖體的生合成，調節pre-rRNA轉錄及幫助核糖體蛋白運回核仁：此與其轉錄後調節相關。因此針對nucleolin的胺基酸序列有許多個可能之磷酯化位置，我們欲純化核萃取液中的蛋白激?，研究nucleolin磷酯化後之生化及其與DNA結合親和力之影響。 首先將核萃取液中nucleolin純化出來，再與具較高磷酯化活性的流出部分進行重組磷酯化試驗。找出的 nualeolin蛋白激?，做進一步純化，並鑑定其對nucleolin磷酯化胺基酸位置為絲胺酸。這種蛋白激?作用之pH值範圍很廣，對熱穩定性不佳，而對其活性促進劑或抑制劑之反應與已知的nuoleolin蛋白激?casein kinaseⅡ相似。這些生化性質可做為純化出此蛋白激?及研究nucleolin功能之參考。 | zh_TW |
| dc.description.abstract | Nucleolin (C23) has been found to be a candidate repressor that binds to a negative cis-element in the AGP promoter region. Nucleolin is one of the major nucleolar proteins in exponentially growing cells and plays a direct role in ribosome assembly, regulating the transcription of pre-ribosomal RNA, and shuttling the ribosomal proteins to the nucleolus. Furthermore, its posttranslational modifications-phosphorylation and proteolysis are related to these functional regulations. Nucleolin has several potential phosphorylation sites in its N-terminal domain. To further understand the regulation of nucleolin phosphorylation, I purify its protein kinase from 129P nuclear extract.
Nucleolin was purified from the nuclear extract by Heparin-Sepharose, Q-Sepharose, and gel filtration column chromatography. The kinase was purified by Q-Sepharose, Mono-S, and gel filtration column chromatography. The partially purified kinase can phosphorylate the serine residue(s) of nucleolin. Further characterization of the nucleolin kinase showed that it has a wide pH optimum, poor heat stability and responds to some stimulators and inhibitors of casein kinase Ⅱ | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:50Z (GMT). No. of bitstreams: 0 Previous issue date: 1994 | en |
| dc.description.tableofcontents | 中文摘要. . . . . . . . .1 英文摘要. . . . . . . . .2 第一章 緒言. . . . . . . . .3 第二章 材料與方法. . . . . . . . .7 一、製備129P細胞株之核萃取液. . . . . . . . .7 二、純化核萃取液內之核仁蛋白Nucleolin及其磷酸激?. . . . . . 9 三、西方點墨法（Western blotting ) . . . . . . . . .11 四、Kinase Assay . . . . . . . . .15 五、Phosphoamino Acid Analysis . . . . . . . . .16 六、V8 Protease 蛋白質水解與蛋白質 N 末端胺基酸定序. . . . . . 17 七、DNA 結合蛋白之分析. . . . . . . . .19 第三章 結果. . . . . . . . .23 附圖. . . . . . . . .30 第四章 討論. . . . . . . . .54 第五章 參考文獻. . . . . . . . .58 | |
| dc.language.iso | zh-TW | |
| dc.title | 純化及鑑定Nucleolin之蛋白激? | zh_TW |
| dc.title | Purification and characterization of Nucleolin Kinase | en |
| dc.date.schoolyear | 82-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 68 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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