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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 吳明寰 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:16Z | - |
| dc.date.available | 2021-07-01T08:17:16Z | - |
| dc.date.issued | 1994 | |
| dc.identifier.citation | Arp, J., Ford, C.M., Palker, T.J., King, E.E. and Dekaban, G.A. 1993. Expression and immunogenecity of the entire human T-cell leukemia virus type I envelpoe protein produced in a baculovirus system. J. Gen. Virol.74:211-222. Baba, E., Nakamura, M., Tanaka, Y., Kuroki, M., Itoyama, Y., Nakano, S. and Niho, Y. 1993. Multiple neutralizing B-cell epitopes of human T-cell leukemia virus type 1 (HTLV-1) identified by human monoclonal antibodies. J. Immunol. 151:1013-1024. Beilke, M.A. 1992. Detection of HTLV-I in clinical specimens. J. Virol. Methods. 40:133-144. Blissard, G.W. and Rohrmann, G.F. 1990. Baculovirus diversity and molecular Biology. Annu. Rew. Entomol. 35:127-155. Chen SI-TI and Compans, R.W. 1991. Oligomerization, transport, and Golgi retention of Punta Toro Virus glycoprotein. J. Virol. 65:5902-5909. Chen, Y.-M.A., Lee, T.H., Samuel, K.P., Okayama, .A., Tachibana, N., Miyoshi, L., Papas, T.S. and Essex, M. 1989. Antibody reactivity to different regions of human T-cell leukemia virus type-I (HTLV-I) gp61 in infected people. J. Virol. 63:4952-4957. Chisholm, G.E. and Henner, D.J. 1988. Multiple early transcrips and splicing of the Autographa californica nuclear polyhedrosis virus IE- 1 gene. J Virol. 62:3193-3200. Clapman, P., Nagy, K. and Weiss, R.A. 1984. Pseudotypes of human T-cell leukemia virus type I and II neutralization by patients’ sera. Proc. Natl. Acad Sci. 81:2886-2889. Copeland, C.S., Zimmer, K.P., Wanger, K.R., Healey, G.A., Mellman, I. and Helinius, A. 1988. Folding, Trimerization, and Transport Are Sequential Events in the Biogenesis of Influenza Virus Hemagglutinin. Cell 53:197-209. Copeland, T.D., Tsai, W.P., Kim, Y.D. and Oroszlan, S. 1986. Envelope proteins of human T cell leukemia virus type I: characterization by antisera to synthetic peptides and identification of a natural epitope. J Immunol. 137:2945-2951. Dewar, R.L., Vasudevachari, M.B., Natarajan, V. and Salzman, N.P. 1989. Biosynthesis and processing of Human Immunodeficiency Virus Type I enveloppe glycoprotein: effects of monensin on glycosylation and transport. J Virol. 63:2452-2456. Dickson, C., Eisenman, R., Fan, F., Hunter, E. and Teich, N. 1982, Protein biosynthesis and assembly. In RNA tumor viruses, pp.513-646. Cold Sprino Harbor Lab. Eiden, M., Newman, M., Fisher, A.G., Mann, D.L., Howley, P.M. and Reitz, M.S. 1985. Type I human T-cell leukemia virus small envelope protein expressed in mouse cells by using a bovine papilloma virus-derived shuttle vector. Mol. Cell. Biol. 5:3320-3324. Elbein, A.D. 1987. Inhibitors of the biosynthesis and processing of N-linked oligosaccharide chains. Annu. Rev. Biochem. 56:497-534. Elbein, A.D. 1991. Glycosidase inhibitors: inhibitors of N-linked oligosaccharide processing. The FASEB journal 5:3055-3063. Ford, C.M., Arp, J., Palker, T.J., King, E.E. and Dekaban, G.A. 1992. Characterization of the antibody response to three different versions or the HTLV-I envelope protein expressed by recombinant vaccinia viruses:induction of neutralizing antibody. Virology 191:448-453. Fukudome, K., Furuse, M., Imai, T., Nishimura, M., Takagi, S., Hinuma, Y. and Yoshie, O. 1992. Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 of C33 antigen in HTL V-1-positive T cells. J. Virol. 66:1394-1401. Furuse, M., Fucudome, K., Imai, Toshino., Uwabe, K.I., Hinuma, Y. and Yoshie, O. 1992. Induction of strong homotypic adhension in human T cell lines positive with human T-cell leukemia virus type 1 by monoclonal antibodies to MHC class I and β2-Microglobulin. Cell. Immunol. 143:298-309. Gavalchin, J., Fan, N., Lane, M.J., Papsidero, L. and Poiesz, B.J. 1993. Identification of a putative cellular receptor for HTLV-I by a monoclonal antibody, Mab 34-23. Virology. 194:1-9. Gallagher, P.J., Hennerberry, J.M., Sambrook, J.F. and Gething, M.J.H. 1992. Glycosylation requirements for intracellular transport and function of the Hemagglutinin of Influen.za Virus. J. Virol. 66:7136-7145. Ghisai, H., Kaiwar, R., Nesburn, A.B. and Wechsler, S.L. 1991. Expression of herpes simplex virus type 1 glycoprotein B in insect cells. Virus Research 22:25-39. Ghiasi, H., Kaiwar, R., Nesburn, A.B. and Wechsler, S.L. 1992. Baculovirus expressed glycoprotein G of Herpes Simplex Virus Type 1 partially protects vaccinated mice against lethal HSV- 1 challenge. Virology 190:233-239. Ghisai, H., Kaiwar, R., Nesburn, A.B. and Wechsler, S.L. 1992. Expression of Herpes Simplex Virus Type 1 glycoprotein I in Baculovirus: Preliminary biochemical characterization and protection studies. J Virol.66:2505-2509. Ghiasi, H., Nesburn, A.B. and Wechsler, S.L. 1991. Cell surface expression of herpes simplex virus type I glycoprotein H in recombinant baculovirus-infected cells. Virology 185:187-194. Granados, R.R. and Williams, K.A. 1986. In vivo infection and replication of Baculoviruses. p89-108. in Granados, R.R. and Federici, B.A. (eds.). The biology of baculoviruses. Vol. I. CRC press, 275pp. Guarino, L.A. and Summers, M.D. 1986a. Functional mapping of a trans-activating gene required for expression of a baculovirus delayed-eraly gene. J. Virol. 57:563-571. Guarino, L.A. and Summers, M.D. 1986b. Interspersed homologous DNA of Autographa californica nuclear polyhedrosis virus enhences delayed-early early gene expression. J. Virol.60:215-223. Guarino, L.A. and Summers, M.D. 1987. Nucleotide sequence and temporal-expression of a baculovirus regulatory gene. J. Virol. 61:2091-2099. Guarino, L.A. and Summers, M.D. 1988. Functional mapping of Autographa californica nuclear polyhedrosis virus genes required for late gene expression. J. Virol. 62:463-471. Haseltine, W.A., Sodroski, J.G. and Patarca, R. 1985. Structure and function of the genome of HTLV. Curr. Top. Microbiol. Immunol. 115:177-210. Hattori, S., Kiyokawa. T., Imagawa, K.I., Shimizu, F., Hashimura, E., Seiki, M. and Yoshida, M. 1984. Identification of gag and env gene products of human T cell leukemia virus (HTLV). Virology, 136:338-348. zurHausen, H. 1991. Virus in human cancers. Science. 254:1167-1173. Hawtin, R.E., King, L.A. and Possee, RD. 1992. Prospects for the developement of genetically engineered baculovirus insecticide. Pestic Sci.34:9-15. Hill-Perkins, M.S. and Possee, R.D. 1990. A baculovirus expression vector derived from the basic protein promoter of nuclear polyhedrosis virus. J. gen. Virol. 71:971-976. HinoJ S., Yamaguchi, K., Katamine, S., Sugiyama, H., Amabasaki, T., Kinoshito, K., Yoshida, Y., Doi, H., Tsuji, Y. and .Miyamato, T. 1985. Mother to child transmission of human T-cell leukemia virus type-I. Gann76:474-486. Hiramatsu, K., Masuda, M. and Yushikura, H. 1986. Mode of transmmision of human T-cell leukemia virus type 1 (HTLV-I) in a human promyelocytic leukemia HL60 cell. Int. J. Cancer. 37:601-606. Hjelle, B. 1991. Human T-cell leukemia/lymphoma viruses. Arch. Pathol. Lab. Med. 115:440-450. Hoshino, H., Shimoyama, M., Miwa, M. and Sugimura, T. 1983. Detection of lymphocytes producing a human retrovirus associated with adult T- cell leukemia by sycytium induction assay. Proc. Natl. Acad Sci. USA 80:7337-7341. Hoxie, J.A., Matthews, D.M. and Cines, D.B. 1984. Infection of human endothelial cells by human T-cell leukemia virus type I. Proc. Natl. Acad. Sci. USA 81:7591-7595. Jarvis, D.V., Fleming, J.G.W., Koracs, G.R, Summers, M.D. and Guarino, L.A. 1990. Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably transformed lepidopteran cells. Bio/Technology 8:950-955. Kannagi, M., Koroda M.J., Maeda, V. and Harada, S. 1991. Coexistence of fusion receptors for human T-cell leukemia virus type I (HTLV-I) and Human Immunodeficiency Virus Type I (HTLV-I) on Molt-4 cells. Microbiol. Immunol. 35(9):729-740. Kiyokawa, T., Yoshikura, H., Hattori, S., Seiki, M. and Yoshida, M. 1984. Envelope protein of human T-cell leukemia virus: expression in Escherichia coli and its application to studies of env gene functions. Proc. Natl. Acad Sci. 81:6202-6206. Knight, P. 1991. Baculovirus vectors for making protein in insect cells. ASM News 57:567-570. Koprowski. H., DeFreitas, E.C., Harper, M.E., Sandberg-Wollhaim, M., Sheremata, W.A., Robert-Guroff, M., Saxinger, C.W., Feinberg, M.B., Wong-Staal, F. and Gallo, RC. 1985. multiple sclerosis and human T-cell lymphotropic retroviruses. Nature 318:154-160. Kuga, T., Hattori, S., Yoshida, M. and Taniguchi, T. 1984. Expression of human T-cell leukemia virus type I envelope protein in Saccharomyces cerevisiae. Gene 44:337-340. Kuroki, M., Nakamura, M., Itoyama, V., Tanaka, V., Shiraki, H., Baba, E., Esaki, T., Tatsumoto, T., Nagafuchi, S., Nakano, S. and Niho, Y. 1992. Identification of new epitope recognized by human monoclonal antibodies with neutralizing and antibody-dependent cellular cytotoxicity activities specific for human T cell leukemia virus type 1. J Immunol. 149:940-948. Lanford, R.E., Luckow, V., Kennedy, R.C., Dreesman, G.R., Notvall, L. and Summers, M.D. 1989. Expression and characterization of Hepatitis B Virus Surface Antigen Polypeptides in Insect Cells with a Baculovirus Expression System. J. Virol. 63:1549-1557. Lee, T.H., Coligan, J.E., Homma, T., McLane, M.F., Tachibana, N. and Essex, M. 1984. Human T-cell leukemia virus associated membrane antigen: identity of the major antigens recognized after virus infection. Proc. Natl. Acad. Sci. 81:3856-3860. Lifson, J., Coutre, S., Huang, E. and Engleman, E. 1986. Role of envelope glycoprotein carbohydrate in human immunodeficiency virus (HIV) infectivity and virus-induced cell fusion. Journal of Experimental Medicine. 164:2101-2106. Lillehoj, E.P., Alexander. S.S., Dubrule, C.J., Wiktor, S., Adams, R., Tai, C.-C., Manns, A. and Blattner, W.A. 1990. Development and evaluation of a human T cell leukemia virus type I serological confirmatory assay incorporating a recombinant envelope polypeptide. J. Clin. Microbiol. 28:2653-2658. Maeda, S., Kawai, T., Obinata, M., Fujiwara, H., Horiuchi, T., Saeki, Y., Sato, Y., and Furusawa, M. 1985. Production of human α-interferon in silkworm using a baculovirus vector. Nature 315:592-594. Manns, A. and Blattner, W.A. 1991. The epidemiology of the human T-cell lymphotropic virus type-I and type-II: etiology role in human diseases. Transfusion 31:67-75. Matsuura, V., Harada, S., Suzuki, R, Watanabe, Y., Inoue, Y., Saito. I. and Miyamura, T. 1992. Expression of processed envelope protein ot hepatitis C virus in mammalian and insect cells. J. Virol. 66:1425-1431. McGonigal, T.P., Turon, M.C., Komar-Hartnett, K., Kister, S.E. and Smith, R.E. 1992. Expression of the gene coding for the major outer capsid protein of SA-11 rotavirus in a baculovirus system. Virus Research 23:135-150. Nagy, K., Clapham, P., Cheingsong-Popov, R. and Weiss, RA. 1983. Human T-cell leukemia virus type I: induction of syncytium and inhibition by patients’ sera. Int. J. Cancer. 32:321-328. Nakamura, H., Eayami, M., Ohta. V., Ishikawa, K., Tsuijimoto, H., Kiyokawa, T., Yoshida, M., Sasagawa, A. and Honjo, S. 1987. Protection of cynomolgus monkeys against infection by human T-cell leukemia virus type-I by immunization with viral env gene products produced in Escherichia coil. Int. J. Cancer 40:403-407. Noraz, N., Benichou, S., Madaule, P., Tiollais, P., Vernant, J.C. and Desgranges, C. 1993. Expression of HTLV-I Env and Tax recombinant peptides in Yeast: Identification of immunogenic domains. Virology193:80-88. Nyunoya, H., Ogura, T., Kikuchi, M., Iwamoto, H., Yamashita, K., Maekawa, M., Takebe, Y., Miyamura, K., Yamazaki, S., Shimoto, K.1990. Expression of HTLV-I envelope protein fused to hydrophobic amino-termonal peptide of baculovirus polyhedrin in insect cellls and its application for serological assays. AIDS Research and Human Retroviruses 6:1311-1321. Okochi, K., Sato, H. and Hinuma. Y. 1984. A retrospective study on transmission of adult T-cell leukemia virus by blood transmission: seroconversion in recipients. Vox. Sang. 46:245-253. O’Reilly, D.R, Miller. L.K. and Luckow, V.A. 1992. Baculovirus expression vectors: A laboratory manual_ W.H. Freeman & Company. Oroszlan, S. and Copeland T.D. 1985. Primary structure and processing of gag and env gene products of human T-c?ll leukemia viruses HTLV-ICR and HTLV-IATK Curr. Top. Microbiol. Immunol. 115:221-233. Paine, E., Gu, R. and Ratner, L. 1994. Structure and expression of the human T-cell leukemia virus type I envelope protein. Virol. 199:331-338. Palker, T.J. 1990. Mapping of epitopes on human T-cell leukemia virus type I envelope glycoprotein. Human Retrovirology: 435-445. Pique, C. and Dokhelar, M.-C. 1991. The HTLV-I envelope: An overview. In: Haseltine and Wong-Staal ed. Genetic structure and regulation of HIV. p.499-510. New York: Raven Press. Pique, C., Pham. D., Tursz, T. and Dokhelar, M.-C. 1992. Human T-cell luekemia virus type I envelope protein maturation process: requirements for syncytium formation. J. Virol. 66:906-913. Pique, C., Pham, D., Tursz, T. and Dokhelar, M.C. 1993. The cytoplasmic domain of the human T-cell leukemia virus type I envelope can modulate envelope functions in a cell type-dependent manner. J. Virol.67:557-561. Plat, E.J., Kani, S.R., Goodman, L.J. and Firestone, G.L. 1992. Differential transport and processing of variant mouse mammary tumor virus glycoproteins. J. Cell. Biochem. 49:425-437. Poiesz. B.J., Ruscetti, F.W., Gazdar, M.A., Bunn, P.A., Minna, J.D. and Gallo, R.C. 1980. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc. Natl. Acad. Sci. 77:7415-7419. Rasmussen, L., Greenwood, J.D. and Gonda, M.A. 1992. Expression of bovine immnunodeficiency-like virus envelope glycoproteins by a recombinant baculovirus in insect cells. Virology 186:551-561. Robert-Guroff, M., Weiss, S.H., Giron, J.A., Jennings, A.M., Ginsderg, H.M., Margolis, I.B., Blattner, W.A. and Gallo. R.C. 1986. Prevalence of antibodies to HTLV-I, -II and -III in intravenous drug abusers from an AIDS endemic region. .J. Ame. Med. Assoc. 255:3133-3137. Rosenblatt, J.D., Chen, I.S.Y. and Wachsman, W. 1988. Infection with HILV-I and HTLV-II: evolving concepts. Seminar in Hematol. 25:230-246. Sarma. P.S. and Gruber. J. 1990. Human T-cell lymphotropic viruses in human diseases. J. Natl. Cancer. Inst. 82:100-106. Samuel, K.P., Lautenberger, J.A., Jorcyk, C.L., Josephs, S., Wong-Staal, F. and Papas, T.S. 1984. Diagnostic potential for human malignancies of bacteria produced HTLV-I envelope protein. Science 226:1094-1097. Seiki, M., Hattori, S., Hirayama, Y. and Yoshida M. 1983. Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in Leukemia cell DNA. Proc. Natl Acad, Sci. 80:3618-3622. Seki, M., Sashiyama, H., Hayami, M. and Shida, H. 1990. Intracellular processing and immunogenecity of the envelope proteins of human T-cell leukemia virus type I that are expressed from recombinant vaccinia viruses. Virus Gene 3:3,235-249. Shida H., Hinuma, Y., Hatanaka, M., Morita, M., Kidokora, M., Suzuki, K, Maruyama, T., Takahashi-Nishimaki, F., Sugimato, M., Kitamura, R, Miyazawa, T. and Hayami, M. 1988. Effects and virulences of recombinant vaccinia viruses derived from attenuated strains that express the human T-cell leukemia virus type I envelope gene. J. Virol.62:4474-4480. Shida, H., Tochikura, T., Sato, T., Konno, T., Hirayoshi, K., Seki, M., Ito, Y., Hatanaka, M., Hinuma, V., Sugimoto, M., Takashi-Nishimaki, F., Maruyama, T., Miki, K., Suzuki, K., Morita, M., Sashiyama, H. and Hayami, M. 1987. Effect of the recombinant vaccinia viruses that express HTLV-I envelope gene on HTLV-I infection. EMBO J. 6:3379-3384. Sisk, W.P., Bradley, J.D., Kingsley, D. and Patterson, T.A. 1992. Deletion of hydrophobic domains of viral glycoproteins increases the level of their production in Escherichia coli. Gene 112:157-162. Smith. G.E., Summers, M.D. and Fraser, M.J. 1983. Production of human B-[SCcl] interferon in insect cells infected with a baculovirus expression vector. Mol. Cell. Biol. 3:2156-2165. Smith, M.R. and Greene, W.C. 1991. Molecular biology of the type I human T-cell leukemia virus (HTLV-I) and adult T-cell leukemia. J. Clin. Invest. 87:761-766. Sommmerfelt, M.A. and Weiss, RA. 1990. Receptor interference groups of 20 retroviruses plating on human cells. Virology 176:58-69. Tajima, K., S. Tominaga, T. Suchi, T. Kawagoe, H. Komoda, Y. Hinuma, T. Oda, K. Fujita. 1982. Epidermiological analysis of the distribution of antibody to adult T-cell leukemia virus-associated antigen: possible horizontal transmission of adult T-cell leukemia virus. Gann73:893. Takatsuki, K., Uchiyama, J., Sagawa, K and Yodoi, J. 1977. Topics in Hematology. (Eds. Seno, S., Takaku, F. and Irino, S.) 73-77. Tanaka, Y., Zeng, L., Shiraki, H., Shida, H. and Tozawa, H. 1991.Identification of a neutralization epitope on the envelope gp46 antigen of human T cell leukemia virus type I and induction of neutralizing antibodies by peptide immunization. J Immunol. 147:354-360. Thiem, S.M. and Miller, L.K. 1989. Identification, sequence, and transcriptional Autographa californica nuclear polyhedrosis virus. J. Virol.63:2088-2018. Trimble, R.B. Maley, F. 1984. Optimizing hydrolysis of N-linked high-mannose oligosaccharides by Endo-B-N-acetylglucosaminidase H. Anal. Biochem. 141:515-522. Vlak, J.M. and Rohramann, G.F. 1985. The nature of polyhedrin. p489-452. In Viral insecticides for biological control. Maramorsch, K and Sherman, K.E. (eds.). Academic press, New York. 809 pp. Vernet, T., Tessier, D.C., Richardson, C., Laliberte, F., Khouri, H.E., Bell, A.W., Storer, A.C. and Thomas, D.Y. 1990. Secertion of functional papain precursor from insect cells. J Biol. Chem. 265:16661-16666. Walker, B.D., Kowalski, M., Goh, W.C., Kozarsky, K., Krieger, M., Rosen, C., Rohrschneider, L., Haseltine, W.A. and Sodroski, J. 1987. Inhibition of human immunodeficiency virus syncytium formation and virus replication by castanospermine. Proc. Natl. Acad. Sci. USA 84:8120-8124. Wells, D.E. and Compans, R.W. 1990. Expression and characterization of a functional human immunodeficiency virus envelope glycoprotein in insect cells. Virology 176:575-586. Whitford, M. and Faulkner, P. 1992. A structural polypeptide of the Baculovirus Autographa californica Nuclear Polyhedrosis Virus contains 0-linked N-acetylglucosamine. J. Virol. 66:3324-3329. Wong-Staal, F. and Gallo, R.C. 1985. Human T-lymphotropic retroviruses. Nature 317:395-403. Yoshida, M., Hattori, S. and Seiki, M. 1985. Molecular biology of human T-cell leukemia virus associated with adult T-cell leukemia. Curr. Top. in Microbiol. Immunol. 115:157-175. 高賢源（1993），「人類第一型嗜T細胞病毒外套膜蛋白之表現、定性與應用」.台大醫學院醫事技術所碩士論文。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76016 | - |
| dc.description.abstract | 以桿狀病毒表現載體系統（baculovirus expression vector system）的兩個不同時期之?動子-鹼性蛋白?動子（basic protein promoter）及多角體蛋白?動子（polyhedrin protein promoter)，皆能夠成功地表現出完整的HTLV-I外套膜蛋白序列。但由酵素連結免疫分析、放射標定或西方點墨法得知，HTLV-I外套膜蛋白中之gp21蛋白僅能累積於秋行軍蟲細胞內而無法分泌至培養液中。由鹼性蛋白?動子所生產之外套膜蛋白有較完全的醣化作用，使未經醣化及部份醣化的蛋白幹擾觀察之現象得以減少。此外，以免疫螢光法觀觀測的結果可知，較完全醣化之外套膜蛋白能大量地聚集於秋行軍蟲的細胞表面，因此醣化應與外套膜蛋白的運送有關。 醣化作用抑制劑加tunicamycin明顯地抑制了HTLV-I穿透膜蛋白gp21在昆蟲細胞之生成，glucosidase的抑制劑deoxynojirimycin亦可阻止小分子量膜蛋白產物生成，由此可推測醣化作用與膜蛋白的切割有關。此外，以Endoglycosidase H及N-Glycosidase F處理蛋白產物後得知，核多角體蛋白?動子生產之46?62kDa附近之醣蛋白屬於high-mannose醣化作用之產物，而鹼性蛋白?動子生產之27、30及46?62kDa醣蛋白亦屬於high-mannose的醣蛋白。 利用酵素連結免疫分析偵測核多角體蛋白及鹼性蛋白?動子生產之HTLV-I外套膜蛋白之相對產量，可知後者產量較低。綜合各項優缺點，可作為使用此兩種?動子時取拾的參考。 | zh_TW |
| dc.description.abstract | The entire envelope gene of human T cell lymphotropic virus type I (HTLV-I) has been successfully expressed in insect cell by using two baculovirus vectors, under the control of the polyhedrin and basic protein promoters respectively. Enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis revealed that the recombinant envelope proteins were accumulated within the cells. Even with radioimmunoprecipitation analysis, the recombinant envelope proteins were still not detectable in the culture media of recombinant baculovirus infected Sf 21-AE cells. The glycosylated precursor was detected, which was then cleaved into two mature products of respective molecular weights 46kDa and 21kDa. In comparison to polyhedrin promoter-driven HTLV-I envelope gene expression, the glycosylation of expressed HTLV-I envelope proteins driven by basic protein promoter were more completely glycosylated. The more completely glycosylated HTLV-I envelope proteins tended to accumulated abundantly on the insect cell surface. Thus it is most likely that the glycosylation is important for the HTLV-I envelope proteins being transported to the cell surface. Treatment of a N-linked glycosylation inhibitor (tunicamycin) and a glycosid?se inhibitor (deoxynojirimycin) has been shown to result in the absence of 21kDa HTLV-I envelope protein in recombinant baculovirus infected Sf 21-AE cells. The evidences suggest that the HTLV-I envelope protein precursor can be cleaved only after glycosylation. Endoglycosidase H and N-glycosidase F treatment revealed a high-mannose type glycosylation having occurred in 46?62kDa proteins produced by polyhedrin promoter-driven expression and the 27,30 and 46?62kDa proteins by basic protein promoter-driven expression. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:16Z (GMT). No. of bitstreams: 0 Previous issue date: 1994 | en |
| dc.description.tableofcontents | 壹、中文摘要……………1 貳、英文摘要……………2 參、緒言與前人研究……3 肆、實驗材料與方法……17 伍、實驗結果……………27 陸、實驗討論……………36 柒、結論…………………45 捌、參考資料……………47 附圖………………………57 附錄………………………79 | |
| dc.language.iso | zh-TW | |
| dc.title | 以GP21及GP46單株抭體分析昆蟲細胞生?人類第一型T細胞白血病/淋巴瘤病毒外套膜蛋白特性 | zh_TW |
| dc.title | Characterization of Human T-cell Leukemia/Lymphoma Virus Type I (HTLV-I) Envelope Proteins produced in Insect Cells by using GP46 and GP21 Monoclonal Antibodies | en |
| dc.date.schoolyear | 82-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 79 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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