請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75999完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Shu 一 Huei wang | en |
| dc.contributor.author | 王琡惠 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:07Z | - |
| dc.date.available | 2021-07-01T08:17:07Z | - |
| dc.date.issued | 1993 | |
| dc.identifier.citation | Anderson, K.V. and Lengyel, J.A. (1979) Rates of synthesis of the major classes of RNA in Drosophila embryos. Dev. Bid., 70:217-231. Bendig, M.M. and Williams, J.G. (1983) Replication and expression of Xenopus laevis genes injected into fertilised Xenopus eggs. Proc. Natl. Acad. Sci. USA, 80:6197—6201. Brandhorst, B.P. (1980) Simultaneous synthesis,translation, and storage of mRNA including histone mRNA including histone mRNA in sea urchin eggs. Dev. Biol., 79: 139—148. Brem, G., Brenig, B., Horstgen—Schwark, G. and Winnacker, E.L. (1988) Gene transfer in tilapia(Oreochromis niloticus). Aquaculture, 68:209-219. Chong, S.S.C. and Vielkind, J.R. (1989) Expression and fate of CAT reporter genes microinjected into fertilized medaka(Oryzias latipes) eggs in the form of plasmid DNA, recombinant phage particles and its DNA. Thero. Appl. Genet., 78:369—380. Chourrout, D., Guyomard, R. and Houdebine, L.M. (1986) High efficiency gene transfer in rainbow trout (Salmo gairdneri Rich.) by microinjection into egg cytoplasm. Aquaculture, 51:143-150. Chuang, N.N. (1990) A neutral beta-galactosidase from the hepatopancreas of the shrimp penaeus monodon (crustacea: decapoda) : dimeric and sialyated. Comp. Biochem. Physiol., 96B(4):747-751. Clegg, K.B. and Piko, L. (1983) Poly(A) length, cytoplasmic adenylation and synthesis of poly(Ay RNA in early mouse embryos. Dev. Biol., 95:331-341. Dunham, R.A. and Eash, J. (1987) Transfer of the metallothionein-human growth hormone fusion gene into channel catfish. Trans. Amer. Fisheries Soc., 116:87—91. Eikcen, H.G., Njolstrad, P.R., Molven,A. and Fjose,A. (1987) A zebrafish homeobox-containing gene with embryonic transcription. Biochem. Biophys. Res. Commun., 149:1165—1171. Etkin, L.D. and Balcells, S. (1985) Transformed Xenopus embryos as a transient expression sytem to analyze gene expression at the midblastula transition. Dev. Biol., 108:173—178. Fjose, A., Molven, A. and Eiken, H.G. (1988) Molecular cloning and characterization of homeobox-containing genes from atlantic salmon. Gene 62:141—152. Friedenreich, H. and Schartl, M. (1990) Transient expression directed by homologous and heterologous promoter and enhancer sequences in fish cells. Nucleic Acids Res. 18(1l):3299— 3305. Gordon, J.W., Scangos, G.A., Plotkin, D.J., Barbosa, J.A. and Ruddle, F.H. (1980) Genetic transformation of mouse embryos by microinjection of purified DNA. Proc. Natl. Acad. Sci. USA, 77:7380—7384. Hammer, R. E., Pursel, V.G., Rexroad, C.E.Jr., Wall,R.J., Bolt, D.J., Ebert, K.M., Palmiter,R.D. and Brinster,R.L. (1985) Production of transgenic rabbits, sheep and pigs by microinjection. Nature, 315:680—683. Hannig, G., ottilie, S. and Schartl, M. (1991) Conservation of structure and function of the c— yes and fyn genes in lower vertebrates. oncogene, 6:361—369. McEvoy, T.G., Stack, M., Keane, B., Barry, T., Sreenan, J.M. and Gannon, F. (1988) The expression of a foreign gene in salmon embryos. Aquaculture, 68:27—38. McMahon, A.P., Novak, T.J., Britten, R.J. and Davidson, E.H. (1984) Inducible expression of a cloned heat shock fusion gene in sea urchin embryos. Proc. Acad. Sci. USA, 81:7490—7494. McMahon, A.P., Flytzanis, C.N., Hong-Evans, B.R., Katula, K.S., Britten, R.J. and Davidson, E.H. (1985) Introduction of cloned DNA into sea urchin egg cytoplasm: replication and persistence during embryogenesis. Dev. Biol., 108:420—430. Nemoto, N., Kodama, K., Tazawa, A., Masahito, P. and Ishikawa, T. (1986) Extensive sequence homology of the goldfish ras gene to mammalian ras genes. Differentiation, 32:17-23. Newport, J. and Kirschner, M. (1982a) A major developmental transition in early Xenopus embryos: I. Characterization and timing of cellular changes at the midblastula stage. Cell, 30:675—686. Newport, J. and Kirschner, M. (1982b) A major developmental transition in early Xenopus embryos:II. Control of the onset of transcription. Cell, 30:687—696. Njolstad, P.R., Molven, A., Apold, J. and Fjose, A., (1990) The zebrafish homeobox gene hox—2.2: transcription unit, potential regulatory regions and in situ localization of transcripts. EMBO J., 9:515—524. Ozato,K., Inoue, K. and Wakamatsu, Y. (1989) Transgenic fish: biological and technical problems. Zool. Sci., 6:445—457. Ozato, K., Kondoh, H., Inohara, H., Iwamatsu, T., Wakamatsu, Y. and Okada, T.S. (1986) Production of transgenic fish: introduction and expression of chicken δ-crystallin gene in medaka embryos. Cell Differ., 19:237—244. Rubin, G.M. and Spradling, A.C. (1982)Genetic transformation of Drosophila with transposable element vactors. Science, 218:348-353. Ruscofli, S. and Schaffner, W. (1981) Transformation of frog embryos with a rabbit β-globin gene. Proc. Natl. Acad. Sci. USA, 78:5051—5055. Shuman, R.M. (1991) Production of transgenic birds. Experientia, 47:897—902. Steller, H. and Pirrotta, V. (1984) Regulated expression of genes injected into early Drosophila embryos. EMBO J., 3:165—173. Stuart, G.W., McMurray, J.V. and Westerfield, M. (1988) Replication, integration and stable germ- line transmission of foreign sequences injected into early zebrafish embryos. Development, 103:403—412. Suemori, H., Kadokawa, K., Goto, K., Araki, I., Kondoh, H. and Nakatsuji, N. (1990) A mouse embryonic stem cell line showing pluripotency of differentiation in early embryos and ubiquitous β—galactosidase expression. Cell Differ. Develop., 29:181—186. Surani, M.A., Reik, W. and Allen, N.D. (1988) Transgenes as molecular poobes for genomic imprinting. Trends. Genet., 4:59—62. Ueno, K., Hiramoto, Y., Hayashi, S. and Kondoh, H.(1987) Introduction and expression of recombinant β—galactosidase genes in cleavage stage mouse embryos. Develop. Growth and Differ., 30(l):61-73. Van Beneden, R.J., Watson, D.K., Chen, T.T., Lautenberger, J.A. and Papas, T.S. (1986) cellular myc (c—myc) in fish (rainbow trout): Its relationship to other vertebrate myc genes and to the transforming genes of the MC29 family of viruses. Proc. Natl. Acad. Sci. USA., 83:3698- 3702. Vielicind, J.R., Chong, S.S.C., Stuart, G.W. and Sadaghiani, B. (1988) Transgenic medaka and zebrafish systems: transient and inherited expression of recombinant CAT reporter gene microinjected into fertilized eggs. in: Schroeder, H.J. (ed) New trends in ichthyology. Parey. Munich (in press) Wilt, F,H. (1964) Ribonucleic acid synthesis during sea urchin embryogenesis. Dev. Biol., 9:299-313. Winkler, C., Vielkind, J.R. and Schartl, M. (1991) Transient expression of foreign DNA during embryonic and larval development of the medaka fish(Oryzias latipes). Mol. Gen. Genet., 226:129—140. Woodland, H.R. and Graham, C.F.(1969) RNA synthesis during early development of the mouse. Nature(Lond.), 221:327—332. Zhang, P., Hayat, M., Joyce, C., Gonzalez—Villasenor, L.J., Lin, C.M., Dunham, R., Chen, T.T. and Powers, D.A. (1990) Gene transfer, expression and inheritance of pRSV-rainbow trout GH-cDNA in the common carp, Cyprinus carpio (Linnaeus). Mol. Repro. Dev., 25:3-13. Zhu, Z., Li, G., He, L. and Chen, S. (1985) Novel gene transfer into the fertilized eggs of goldfish (Carassius auratus L. 1758). Z. Appl. Ichthyol., 1:31—34. Zhu, Z., Xu, K., Li, G., Xie, Y. and He, L. (1986) Biological effects of human growth hormone gene microinjected into the fertilized eggs of loach Misgurnus anguillicaudatus (Cantor). Kexue Tongbao, 31:988—990. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75999 | - |
| dc.description.abstract | 將三種不同病毒的基因控制區，Rous sarcoma virus ( pmiwZ ）、Cytomegalovirus immediate-early gene ( pCAGGS-lacZ）、Moloney murine leukemia virus ( pMoZtk) ，接上reporter gene ( lacZ gene），分別利用顯微注射法，注入稻田魚的卵細胞中，再用X-gal的組織化學染色法，來測知此基因在稻田魚中的暫時性表現。結果顯示：( 1 ) ．注射後的卵，人工受精率約為43~72%，而胚胎發育至第24hr的存活率為40~65%:與對照組（分別為76~ 85%與72~82%）相比，都有顯著性降低。（2） ．以南方氏墨點法（Southern blot）來確認 reporter gene 的存在，結果在轉殖 pMoZtk DNA 片段的2hr、3hr、6hr 的胚胎出現模糊延伸的長條狀反應。（3 ） ．此三種DNA均在胚胎發育至囊胚期 ( blastula stage）時開始被表現，其中pMoZtk較晚，約在囊胚後期才出現；且在2天後的胚胎就都不再出現。因此lacZ 基因表現的型式，與病毒基因的調控區域及胚胎發育的時期相關。而分佈的形式，則為鑲嵌型點狀出現於胚胎中。（4 ） ．若注射沒lacZ encoding region的DNA 片段，則無法造成胚胎有藍色出現的結果；可知酵素呈色反應確是由轉殖基因所造成。（5 ) ．競爭性的第二種DNA的加人，可造成基因提早6hr表現，故推測胚胎控制基因轉錄開始時間的方式，可能與Xenopus相同，屬於titration model。 | zh_TW |
| dc.description.abstract | Three different regulatory regions of viral genes , rouse sarcoma virus (pmiwZ), cytomegalovirus immediate-early gene (pCAGGS-lacZ), moloney murine leukemia virus (pMoZtk) microinjected into medaka oocytes. Histochemistry method were used to detect the transition expression of lacZ gene during early embryogenesis. Results indicated: (I).the rate of fertilization and survival (24hr after fertilization) of injected oocytes were 43－72% and 40-65%, respectively. Compared to control group (76-85% and 72-82% for fertilization and survival rate, respectively), microinjection cause a clearly decrease in fertilization and survival rate. (2). Southern blotting revealed that there was a diffused band shown at 2hr, 3hr and 6hr-old injected pMoZth embryos’ genome. (3). The initiation of lacZ gene expression was at mid-blastula stage of embryos injected either pmiwZ or pCAGGS-lacZ, but for embryos injected pMoZtk the expression initated at the late blastula stage. All the expression then disappeared in 2 days-old embryos. The timing of the gene expression was related to the regulatory region of viral gene and the stage of embryonic development. The pattern of gene expression was mosaic. (4). Injection of DNA fragment without containing lacZ gene encoding region failed in blue spots appearance. This result indicated the positive response was controlled by transferred lacZ gene. (5). Co-transferred the competitive DNA(pMT-X) and reporter gene (pmiwZ) showed that the expression of lacZ gene was 6hr earlier than injected lacZ gene alone. This suggested that the mechanism of controlling the gene transcription in medaka embryos maybe the same as the titration model reported in Xenopus embryos. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:07Z (GMT). No. of bitstreams: 0 Previous issue date: 1993 | en |
| dc.description.tableofcontents | 摘要. . . . . . . . . . . . .1 Abstract . . . . . . . . . . . . .2 壹 前言. . . . . . . . . . . . .3 貳 材料與方法. . . . . . . . . . . . .6 一、 顯微注射的DNA片段. . . . . . . . . . . . .6 ( A ）、 pmiwZ ( B ）、 pCAGGS-lacZ ( C ）、 pCAGGS-lacZ不含lacZ基因的質體 ( D ）、 pMoZtk ( E ）、 pMT-X 二、 pCAGGS-lacZ不含lacZ 基因的質體構築. . . . . . . . . . . . .7 ( A )、 pCAGGS 的構築流程 ( B )、 大腸桿菌通透性細胞的製備 三、顯微注射所使用DNA的製備. . . . . . . . . . . . .9 ( A )、大量質體 DNA 的抽取 ( B )、氯化銫梯度離心法 ( C )、用限制?切質體DNA成線型並回收純化 四、顯微注射作基因轉殖. . . . . . . . . . . . .11 ( A ) 稻田魚的飼育 ( B ) 卵母細胞的收集 ( C ) 顯微注射 ( D ) 卵母細胞的培育和人工受精 五、稻田魚中轉殖基因的偵測. . . . . . . . . . . . .12 ( A ）genomic DNA的抽取 ( B ）DNA轉印NC paper上 ( C ）prehybridization ( D ）探針DNA的製備 ( E ）hybridization ( F ）washing and autoradiography 六、分析細lacZ基因的表現. . . . . . . . . . . . .14 ( A )、受精後不到一天及一天的胚胎 ( B )、受精後一天及一天以後的胚胎 參、結果. . . . . . . . . . . . .16 顯微注射外來DNA對卵細胞及胚胎的影響. . . . . . . . . . . . .16 外來基因存在於早期胚胎中的型式. . . . . . . . . . . . .16 轉殖基因在形態正常胚胎中的表現. . . . . . . . . . . . .17 轉殖基因在形態不正常胚胎中的表現. . . . . . . . . . . . .18 去除lacZ基因的DNA片段轉殖的表現 . . . . . . . . . . . . .19 加人另一種高濃度DNA時lacZ基因的表現. . . . . . . . . . . . .20 肆、討論. . . . . . . . . . . . .21 Reference. . . . . . . . . . . . . 30 Table and Figure . . . . . . . . . . . . .35 誌謝 . . . . . . . . . . . . .58 附錄一 . . . . . . . . . . . . .59 附錄二 . . . . . . . . . . . . .60 | |
| dc.language.iso | zh-TW | |
| dc.title | 顯微注射lacZ基因在稻田魚早期胚胎發育中的暫時性表現 | zh_TW |
| dc.title | The transition expression of lacZ gene microinjected into medaka (Oryzias latipes) oocytes during early embryonic development | en |
| dc.date.schoolyear | 81-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 66 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
文件中的檔案：
沒有與此文件相關的檔案。
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。