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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Yeong-Chang Chang | en |
| dc.contributor.author | 張永昌 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:17:02Z | - |
| dc.date.available | 2021-07-01T08:17:02Z | - |
| dc.date.issued | 1993 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75988 | - |
| dc.description.abstract | SCD2 (Stearoyl CoA desaturase2)的負調節序列PRE(Preadipocyte repressor element)的序列和一普遍存在之核內因數YY-1 的結合序列甚為相似。YY-l對轉錄的調控有時扮演促進子的角色，有時則扮演抑制子的角色，端視所涉及的基因以及結合序列而定。
在本論文中，我經由比較UCR(upstream conserved region)結合序列CGCCATTTT與SCD2?動子PRE結合序列AGCCATTTC。發現一個共同因數可以結合到這二者的結合序列上，從競爭性的原性電泳膠遲緩試驗中，我已經證實 UCR和SCD2的序列專一性的確很相似。更進一步研究顯示UCR和SCD2上的結合因數具有相同抗原性，於是，我們可以下一個結論：普遍存在之核內因數，YY-1，可以認知SCD2?動子上的PRE。 至於YY-1在SCD2上扮演何種角色，尚需進一步實驗才得以知曉。 | zh_TW |
| dc.description.abstract | The sequence of a negative cis-element, PRE (preadipocyte repressor element) of SCD2 (stearoyl CoA desaturase 2) is closely related to the binding sites for a ubiquitous cellular factor, YY-1 (NF-E1,δor UCRBP). YY-1 appears to be somewhat unique in that it can exert either a positive or a negative control over transcription, depending on the genes and binding motifs involved. In this thesis, I compared UCR (upstream conserved region) core sequence CGCCATTTT with SCD 2 promoter binding site PRE AGCCATTTC. I have detected common factors that can bind to these two motifs. From EMSA competition assay, I have confirmed that the sequence specifity of UCR and SCD 2 binding factors are similar to YY-1. Further studies have shown that both UCR and SCD 2 binding factors share antigenicity with YY-1.We conclude that the ubiquitious transcriptional factor ,YY-1 ,can recognize PRE of SCD 2. Further experiments are needed to clarify the functional role of YY-1 on the regulation of SCD 2 expression. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:17:02Z (GMT). No. of bitstreams: 0 Previous issue date: 1993 | en |
| dc.description.tableofcontents | 致謝 1 縮寫表及中文翻譯 2 目錄 4 一、中英文摘要(Abstract) 6 二、緒言(Introduction) 8 三、材料與實驗方法(Materials and Methods) 11 1．細胞培養 11 2．細胞核萃取液之製備 11 3．原性電泳膠遲緩(EMSA)試驗 12 4．西南方轉拓法 14 5．核酸定序 15 (A) 單股核酸定序 (B) 雙股核酸定序 6．質體的設計 16 (A) 寡核酸多元化的方法 (B) 質體特性介紹 (C) PCAT-SCD2質體接法 (D) PCAT-UCR質體接法 7．質體的抽取 17 (A) 小量 (B) 大量 8．基因轉染試驗 17 9．氯?乙醯轉換?CAT試驗 18 10．在EMSA中，所用的探針以及競爭子的結合序列 18 四、結果(Result) 20 五、討論(Discussion) 22 六、附圖(Figure) 26 七、附錄(Appendix) 45 八、參考文獻(Reference) 54 | |
| dc.language.iso | zh-TW | |
| dc.title | 普遍存在之核內因數，YY-1，可以認知SCD2?動子上的PRE | zh_TW |
| dc.title | Ubiquitous transcriptional factor,YY-1,can recognize PRE (preadipocyte repressor element) of SCD2 (Stearoyl CoA desaturase 2) gene | en |
| dc.date.schoolyear | 81-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 59 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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