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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 陳逸夫 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:16:57Z | - |
| dc.date.available | 2021-07-01T08:16:57Z | - |
| dc.date.issued | 1993 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75980 | - |
| dc.description.abstract | 以市售桿狀病毒轉形試劑組所得之重組病毒比例，並非如預期的接近百分之百，因此擬得純系化之重組病毒及較高之重組蛋白產量，至少須經兩次以上病毒純化。接著，取純化過並載有B型肝炎表面抗原M protein基因之重組病毒，與兩株僅載有S protein基因之重組病毒分別感染昆蟲細胞，發現S protein幾乎不分泌至培養液，絕大部分累積在細胞內；反觀在感染後四十八小時即於培養液中偵測到相當量之分泌性M protein。此外，當昆蟲細胞中同時存在M與S protein時，M protein將有抑制S protein分泌之可能。 | zh_TW |
| dc.description.abstract | The fraction of recombinant virus which was obtained from cotransfection fluids using commercial transfection kit was not nearly 100% as expected.So purified recombinants and higher recombinant protein production could only be approached when undergone virus purification twice.Then we analyzed the secretion of HBV M and S proteins from three recombinant viruses containing either M or S protein gene. The results indicated that the S proteins were predominantly accumulated in the insect cells and were inefficiently secreted into the medium.In contrast, there were comparatively high level of the M proteins in the medium two days after infection.On the other hand, the secretion of the S proteins were presumably inhibited by the presence of the M proteins. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:16:57Z (GMT). No. of bitstreams: 0 Previous issue date: 1993 | en |
| dc.description.tableofcontents | 壹、緒言……………………………………1 一、桿狀病毒表現載體系統……………………………………1 二、B型肝炎表面抗原之基因結構、功能與分泌作用……………………………………4 三、本論文研究主題……………………………………7 貳、材料與方法……………………………………9 材料……………………………………9 方法……………………………………10 一、重組病毒之純化……………………………………10 二、分析病毒純化對重組蛋白產量之影響……………………………………11 三、以酵素免疫分析法（EIA）測定三株重組病毒細胞內外HBsAg產量……………………………………11 四、三種去氫酵素活性之測定……………………………………12 a．Malate dehydrogenase……………………………………12 b．Lactate dehydrogenase……………………………………13 c．Isocitrate dehydrogenase……………………………………14 五、以免疫沉澱法（immunoprecipitation）分析細胞內外HBsAg產量……………………………………14 參、結果……………………………………16 一、重組病毒之純化……………………………………16 二、病毒純化對重組蛋白產量之影響……………………………………19 三、三株不同重組病毒HBsAg分泌型態之比較……………………………………25 四、以三種去氫酵素分析細胞溶裂變化與B型肝炎表面抗原分泌作用之關係……………………………………29 五、分泌性HBsAg組成之分析……………………………………37 肆、討論……………………………………41 伍、附錄……………………………………49 陸、英文摘要……………………………………53 柒、中文摘要……………………………………54 捌、參考文獻……………………………………55 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用桿狀病毒載體系統研究在昆蟲細胞中B型肝炎表面抗原之合成與分泌作用 | zh_TW |
| dc.title | Synthesis and Secretion of Hepatitis B Surface Antigen (HBsAg) in Insect Cells Using Baculovirus Expression Vectors | en |
| dc.date.schoolyear | 81-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 65 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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