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標題: | 銅綠微囊藻在水庫中大量繁殖之生態因數的研究 |
作者: | 黃蒨菡 |
出版年 : | 1992 |
學位: | 碩士 |
摘要: | 本論文以分離自翡翠水庫之銅綠微囊藻(Microcystis aeruginosa品系2396)為材料,探討此藻種發生藻華現象之生態生理因數。藻種經純化後培養於室內,形成單細胞,其最佳生長的溫度為34℃,光度為200μEm-2s-1,酸鹼值近於7。在生長之指數期,其細胞內之總有機氮及總磷含量下降,至靜止期,其細胞內磷含量增高,並於末期有有機碳之釋出。而細胞含磷量增加與細胞內反應磷含量之增加有關,而無聚磷酸產生。於指數期末期測得之氮、磷原子數比為22:1。此藻種對硝酸氮、銨氮、尿素之利用效率相同,在硝酸氮濃度為14μg.ml-1,磷酸濃度為2μg.ml-1時生長速率最大,當培養基中氮,磷原子數含量比範圍為11?22時生長最好。對氮及磷皆有過量消耗的情形產生,且以對磷尤甚,於湖水中作不同營養鹽之添加,則添加氮及磷之影響與添加所有重要元素相同,其中又以氮的影響最大。並對照1988年水庫之研究報告,指出於藻華發生時之水域中有大量含氮污染源流入,故對於M. aeruginosa品系2396而言水庫水域中之含氮營養鹽濃度可能是其主要之限制因數。 A strain of Microcystis aeruginosa (No.2396) was isolated from Feitsuiku Reservoir during the bloom of thist species in it. The purpose of this study is to find out the relationship between enviromented factors and mass growth of this species. The characterization of the growth condition of this strain showed that the optimum growth condition was under 34℃, 200 μEm-2s-1 and pH at 7.0. At exponential stage of growth, the intracellular organic carbon and phosphorous content were lower than at other stage. At stationary stage, the intracellular phosphate increased with the release of total organic carbon to the medium. The increase in intracellular phosphate were found to be due to the increase of due to the increase of dissoved reactive phosphate in the cells rather than the accumulation of polyphosphate. At exponential stage, the atomic ratios of intracellular N/P was about 22. M. aeruginosa strain 2396 utilized nitrate nitrogen, ammonium nitrogen and Urea in about the same rate. The maximum growth rates were obtained when the nitrate and phosphate were 14ug.ml-1 and 2ug.ml-1, respectively under this condition, the N/P ratios was 11-22. A luxury consumption was revealed for both elements. An experment conducted with the enrichment of N and/or P in the lake water, from which this strain was isolated showed that N played more important role than P for the growth of this species of phytoplankton. This meets well with the results of the analysis of lake water during the bloom in July of 1988. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75883 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
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