請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75877
標題: | 綠豆幼苗經溫度調馴後在溫度逆境下形態及生理之變化 The Effect of Temperature conditioning for Temperature Stress on Physiological and Morphological Changes in Mungbean Seedlings |
作者: | Shen-Hemg Liu 劉炫亨 |
出版年 : | 1992 |
學位: | 碩士 |
摘要: | 針對位處亞熱帶之台灣多變化的氣候型態所造成的高、低溫逆境及大幅度之氣溫變化,利用綠豆(Vigna radiata L.)為材料,經過不同之高溫(40℃)或低溫(10℃)馴化(acclimation)後,進行其對於致死溫度(leathal temperature)在生長發育、生理、生化反應上的抗性研究。 將綠豆浸水後於28℃發芽三十小時的綠豆幼苗,分別以10℃或40℃進行一次或兩次之馴化過程,且配合第一次馴化(1°馴化)及第二次馴化(2°馴化)在溫度上(10℃或40℃)的不同,進行高、低溫之溫度調馴(temperature conditioning)處理。處理後的植株,再放入4℃或45℃的生長恆溫箱中(400 umole/m2/s光照12小時/黑暗12小時)生長兩天,然後分別進行完整葉片滲漏液(leakage)之離子導電度(conductivity)、遊離醣(free sugars)及遊離胺基酸(free amino acids)的測定。並進行植株形態之觀察及全蛋白的分析,並以35S-甲硫胺酸(35-methionine)進行蛋白質合成之標誌。最後,經過一週回溫(28℃)生長,測其葉片之光合作用率(photosynthetic rate)。 在葉片受傷害的保護上,給予綠豆幼苗施以不同溫度之1°馴化及2°馴化,對於爾後4℃或45℃逆溫環境之容忍性(tolerance)高於以同一溫度做為1°馴化及2°馴化的實驗組。而其效果又分別對離子滲漏(ionic leakage)或遊離胺基酸(free amino acids)或遊離醣(free sugars)而有所不同。對於光合作用,重複以同一溫度進行1°馴化及2°馴化可保持較佳的光合作用能力。經過SDS-PAGE的電泳分析,有些蛋白質是為高、低溫處理後所誘導之逆溫蛋白(temperature stress proteins, TSPs)。其中,有些逆溫蛋白在適溫時即存在,經高、低溫處理後,量會增加或累積,特稱之為“常備型之TSPs”。TSPs有分子量分別為111.9KD?101KD之高分子蛋白,與60.9KD, 57.7KD, 43.3KD, 39.8KD, 21.9KD等蛋白。其中,43.3KD, 21.9KD兩者屬常備型之TSPs。經由35S-甲硫胺酸標示為TSPs者,計有分子量大小分別為23.2KD, 22KD, 20KD, 19.2KD, 17.6KD, 16.1KD, 15.9KD等蛋白質。 經由本實驗,證實“溫度調馴”(temperature conditioning)可提供植物頗佳的抗逆溫性,與先前一些學者研就之結果一致(47, 64, 65, 75)。 Taiwan was located in subtropical area of earth. When plant grown on Taiwan, it must be to tolerate the changeful climate in Taiwan. The thirty-six-hour-old mungbean (Vigna vadiata L.) were pretreated with primary (1°-)- and secondary (2°-)- acclimation of different low temperature (10℃) and high temperature (40℃). After 1°- and 2°-acclimation, the mungbean seedlings were transfered to growth chamber with low-lethal temperature (10℃) and high-lethal temperature (45℃) for two days. The morphological change of those treated seedlings was observing, and deciding the degree of damage of the treated seedlings. By photosynthetic rate, ionic conductivity, mass of soluble free sugurs, and mass of free amino acids, it's decided the physiological damage. Using SDS-PAGE and the incorporation of 35S-methionine to understand any change of patterns of the proteins in temperature conditioning treatment. Western blotting was used as qualitative analysis. After temperature conditioning treatment, the injury by temperature stress could be reduce in seedlings. When gave mungbean seedlings 1°-and 2°-acclimation with different temperature (10℃ and 40℃), the ability of tolerance on temperature stress was more than the ability of tolerance which were given 1°- and 2°-acclimation with same temperature. The sequence of 1°-acclimation and 2°-acclimation used different temperature will interfer the effects of treatments. The 1°-acclimation of temperature conditioning maybe the major decisive factor, and the 2°-acclimation of temperature be possible the assistant factor. Temperature conditioning treatment could induce some special proteins, called temperature stress proteins (TSPs). By silver stained method decided molecular weight of TSPs are 111.9KD -101KD, 60.9KD, 57.7KD, and 39.8KD. By incorporation of 35S-methionine decided molecular weight of TSPs are 23.2KD, 22KD, 20KD, 19.2KD, 17.6KD, 16.1KD, and 15.9KD. It's certain that temperature conditioning treatment can offer an efficient ability to resist temperature stress in mungbean seedlings. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75877 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
沒有與此文件相關的檔案。
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。