請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75859
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | 蔡孟利 | zh_TW |
dc.date.accessioned | 2021-07-01T08:15:57Z | - |
dc.date.available | 2021-07-01T08:15:57Z | - |
dc.date.issued | 1992 | |
dc.identifier.citation | 1. Lutz Birnbaumer, Joel Abramowitz, and Arthur M. Brown. (1990). Receptor-effector coupling by G proteins. Biochim. Biophys. Acta. 1031:163-224. 2. Liao C.F., Themmen Axel P. N., Joho R., Barberis C., Birnbaumer M., and Birnbaumer L. (1989). Molecular cloning and expression of a fifth muscarinic acetyl- choline receptor. J. Biol. Chem. 284: 7328-7337 3. Stein R., Pinkas-Kuamarski, and R. Sokolovsky M.(1988). Cloned M1 muscarinic receptors mediate both adenylate cyclase inhibition and phosphoinositide turnover. EMBO.J. 7: 3031-3035. 4. Ashkenagi A., Winslow J.W., Peralta E.G., Peterson G.L., Schimerlik M. I., Capon D.J.,and Ramachandran J.(1987). An M2 muscarinic receptor subtype coupled to both adenylate cyclase and phosphoinositide turnover. Science. 238: 672-675. 5. Pinkas-Kuamarski R., Stein R., Zimmer Y., and Sokolovsky M. (1988). Cloned rat M3 muscarinic mediate phospho-inositide hydrolysis but not adenylate cyclase inhibition. FEBS letter. 239:174-178. 6. Peralta E.G., Ashkenazi A., Winslow J.W., Smith D.H., Ramachandran J., and Capon D.J.(1987).Distinct primary structure, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors. EMBO J. 6: 3923-3929. 7. DeGeorge J.J., Pusley A.H., Me Carthy K.D., Lapetina E. G., and Morell P. (1987).Acetylcholine stimulates selective liberation and re-esterification of arachidionate and accumulation of inositol phosphates and glycerophosphoinositol inC62Bglioma cells. J. Biol. Chem. 262:8077-8083. 8. Snider R.M., Mcklnney M., Forray C., and Richelson E. (1984).Neurotransmitter receptors mediate cyclic GMP formation by Involvement of arachidionic acid and lipoxygenase. Proc. Natl. Acad. Sci. 81: 3905-3909. 9. Cole A.E., and Nicoll R.A.(1984). Characterization of a slow cholingeric post-synaptic potential recorded in vitro from rat hippocampal pyramidal cell, S. J. physiol. 352: 173-188. 10. Berg I., Guse A.H., and Gerckin G. (1988). Carbamcoylchline-induced accumulation of inositol mono-, bis, tris-, and tetra- phosphates in isolated cardiac myocytes from adult rats. Biochim. Biophys. Acta.1010: 1100-107 11. Liao C.F., Schlling W.P., Birnbaumer M., and Birmbaumer L.(1990).Cellular responses to stimulation of the M5 muscarinic acetylcholine receptor as seen in mmurine L cells. J. 8101. Chem. 265:11273-11284. 12. 江明聰.(1991). 刺激第五亞型蕈毒乙醯膽鹼受體M5轉殖之小鼠L細胞誘導c-fos和c-jun mRNA累積之研究。私立輔仁大學生物學研究所碩士論文。 13. Robert J. Lefkowitz. (1991). Variations on a theme. Nature. 351:353. 14. Joel Bockaert. (1991). G protein and G-protein-coupled receptor: structure, function and interactions. Current Opinion in Neurobiology. 1: 32-42 15. Brian F., O’Dowd, Robert J. Lefkowitz, and Marc G. Caron. (1989). Structure of the adrenergic and related receptors. Annu. Rev. Neurosci. 12: 67-83. 16. Henrik G. Dohlman, Jeremy Thorner, Marc G. Caron, Robert J. Lefkowitz. (1991). Model systems for the study of seven-transmembrane-segment receptors. Annu. Rev. Biochem. 60: 653-688. 17. Catherine D. Strader, Irving S. Sigal, and Richard A.F. Dixon. (1989). Genetic approaches to the determination of structure-function relationships of G protein-coupled receptor. TiPS. (supplement):26-30. 18. Catherine O. Strader, Irving S. Sigal, and Richard A.F. Dixon. (1989). Structure basis of β-adreriergic receptor function. FASEB 3: 1825-1831. 19. Jeffrey L. Benovic, Michel Bouvier, Marc G. Caron, and Robert J. Lefkowitz. (1988). Regulation of adenylyl cyclase-coupled -adrenergic receptors. Annu. Rev.Cell Biol. 4: 405-428. 20. Robert J. Lefkowitz, William P. Hausdrof, and Marc G. Caron. (1990). Role of phosphorylation in desensitization of the -adrenoceptor. TiPS. 11:190-194. 21. E. C. Hulme, N. J. M. Birdsall, and N. J. Buckley.(1990). Muscarinic receptor subtypes. Annu. Rev. Pharmacol. Toxicol. 30: 633-673. 22. Jurgen Wess, Tom I. Bonner, Frank Dorj, and Mark R. Brann. (1990). Delineation of muscarinic receptor domains conferring selectivity of coupling to guanine nucleotide- binding proteins and second messengers. Mol. Pharma. 38: 517-523. 23. Lechleiter J., Helmiss R., Duerson K., Ennulat D., Nathaniel D., Clapham D., and Peralta E. Distinct sequence elements control the specificity of G protein activation by muscarinic acetylcholine receptor subtype. EMBO J. 9:4381-4390. 24. Jurgen Wess, David Gdula, and Mark R. Brann. (1991). Site-directed mutagenesis of the M3 muscarinic receptor: identification of a series of threonine and tyrosine residues involved in agonist but not antagonist binding. EMBO J. 10: 3729-3734. 25. Esam E. E1-Fakahany, Catherine L. Cioffi. (1990).Molecular mechanisms of regulation of neuronal muscarinic receptor sensitivity. Mem. Biochem. 9:9-27. 26. Kazuko Haga, Tatsuya Haga. (1990). Dual regulation by G proteins of agonist-dependent phosphorylation of muscarinic acetylcholine receptors. FEBS. 268: 43-47. 27. Dalman H. M. and Neubig R. R.(1991). Two peptides from the adrenergic receptor alter receptor G protein coupling by distinct mechanisms. J. Biol. Chem. 266:11025-11029. 28. Higashijima T., Uzu S. Nalsjima, and Ross E. M. (1988). Mastoparan, a peptide toxin from wasp venom, mimic receptors by activating GTP-binding regulatory proteins (G proteins). J. Biol. Chem. 263: 6491-6494. 29. Hausdroff W. P., Hnatowich M., O’Dowd B. F., Caron M. G., and Lefkowitz R. J. (1990).A mutation of the β-adrenergic receptor impairs agonist activation of adenylyl cyclase without affecting high affinity agonist binding. J. Biol. Chem. 265: 1388-1393. 30. Hausdroff W.P., Bouvier M., O’Dowd B. F. Irons G. P., Caron M. G., and Lefkowitz R. J.(1989). Phosphorylation sites on two domains of the β-adrenergic receptor are involved in distinct pathways of receptor desensitization. J. Biol Chem. 264: 12657-12665. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75859 | - |
dc.description.abstract | G蛋白偶合受體其結構上均有穿進穿出細胞膜七次的特徵。有關於其結構和功能關聯性的研究,由於基因定位突變法和混合型突變種的運用,已漸趨明朗。為研究屬於G蛋白偶合受體一員的第五亞型蕈毒乙醯膽鹼受體其結構中和G蛋白作用的位置,以及其受磷酸化調控的機制,我們設計了三株刪除型突變種(刪除氨基酸序列269-290,268-364,269-290)及一株和人類β2腎上腺受體混成的混合型受體(氨基酸序列M5:1-251,β:262-413)。由哺乳動物細胞短暫質體表現法,發現此四株突變種均可表現於老鼠纖維母細胞株Lik的細胞膜上。有關功能上的改變,則需等到這四株突變種的穩定細胞株建立後才能進行。 | zh_TW |
dc.description.abstract | Receptors coupled With G protein share a highly conserved structure transversing membrane seven times by alpha helical segments. The structure —function relationships of receptors are investigated based on the results of either site—directed mutagenesis or the construction of chimeric receptors. For study of the regions of M5 muscarinic acetylcholine receptor to react With the G protein and the possible desensitization mechanisms, We have constructed three deletion receptor mutants (delete the amino acid residues: 269—290,268—364269—448) plus one chimeric receptor between M5 and humen beta—2—adrenergic receptor (amino acid residues M5:1—251, beta—2: 264—413). By using transient expression in mouse Ltk cells, we have noticed that all four constructed mutants could express receptors on cell membrane. The further study on receptor — medicated cell function may wait the stable cell lines of the four mutants to be established. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:15:57Z (GMT). No. of bitstreams: 0 Previous issue date: 1992 | en |
dc.description.tableofcontents | 謝辭. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I 中文摘要. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . II 英文摘要. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . III 一.前言. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 二.材料. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 三.方法. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 四.結果. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 五.討論. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 六.參考文獻. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 七.圖表說明. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 | |
dc.language.iso | zh-TW | |
dc.title | 第五亞型蕈毒乙醯膽鹼受體刪除型及混合型受體之構築及表現 | zh_TW |
dc.title | Construction and Expression of the Deleted and Chimeric M5 Muscarinic Acetylcholine Receptors | en |
dc.date.schoolyear | 80-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 49 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
文件中的檔案:
沒有與此文件相關的檔案。
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。