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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Chung-Chiun Yin | en |
| dc.contributor.author | 尹重君 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:15:52Z | - |
| dc.date.available | 2021-07-01T08:15:52Z | - |
| dc.date.issued | 1991 | |
| dc.identifier.citation | 1. Hoar, W.S., Randall, D.J. & E.M. Donaldson (1983) Fish Physiology Vol IX Reproduction Part A Endocrine Tissues and Hormone Academic press. 2. Dworkin, M.B. & E. Dworkin-Rastl. (1985). Changes in RNA Titrs and Polyadenylation during Oogenesis and Oocyte Maturation in Xenopus laevis. Develop. Biol. 112, 451—457. 3. Van Den Eynde, H., Mazabraud A & H. Dennis. (1989). Biochenical reseach on Oogenesis. RNA accumulation in the Oocyte of the Newt Pleurodeles walti .Development 105, 11—16. 4. Dworkin, M.B, Shrutoqski, A, Baumgarten, M. & E. Dworkin—Rastl (1984). The accumulation of Prominent Tadpole mRNA Occur at the Beginning of Neurulation in Xenopus laevis Embryos. Develop. Biol. 106, 289—295. 5. 曾煥仁與黃火鍊。鯉魚卵生長之研究。科學發展月刊，第四卷 第四期 2306-2315. 6. Denis, H, Picard, B., Maire, M.L.E. & J.C. Clerot (1980). Biochamical Reseach on Oogenesis. Develop. Biol. 77, 218—223. 7. Golden, L. , Scafer, U. & M. Rosbash Accumulation of Individual mRNAs during Oogenesis of Xenous Laevis. Cell 22,835-844. 8. Rebagliati, M.R., Weeks, D.L., Harvey, R.P. & D.A. Melton (1985). Identification and Cloning of Localized Maternal RNAs from Xenopus egg. Cell 42,769-777. 9. Colonna, R. , Cecconi, S., Tatone, C. Mangia F., & R. Buccione (1989). Somatic Cell-Oocyte Interaction in Mouse Oogenesis: Stage-Specific Regulation of Mouse Oocyte Protein Phosphorylation by Granulosa cells, Develop. Biol. 133, 350-308. 10. Gibert S.F., Development Biology, (2nd edi), p772-814 Sinauer Associates, Inc. Sunderland, ,Massachusetts U.S.A. 11. Aberts, B., Bray, D., Lewis, J., Raff, M., Robert, K. & J.D. Waston (1989). Molecular Biology of The Cell, second edition. 12. Agarwal, M.L. & C.A. Culis (1991). The ubiquitin-encoding multigene family of, Linum usitatassimum. Gene 99, 69-75. 13. Redman, K.L. & M. Recheseiner (1989). Identification of the long ubiquitin extension as ribosomal protein S27a. Nature 338, 438-440. 14. Finley, D., Bartel, B., & A. Varshavsky (1989) The tail of ubiquitin precursors are ribosome protein whose fusion to ubiquitin facilitaies ribosome biogenesis. Nature 338, 394-401. 15. Ozkaynak, E., Finkley, D., Solomon, M.J. & A. Varshavsky (1987). The yeast ubiquitin genes: a family of natural gene fusion. EMBO 6, 1429-1439. 16. Taylor, M.V., Gusse, M., Evan, G.I., Dathan, N., & M. Mechali (1986). Xenopus myc protooncogene during development: expression as a stable maternal mRNA uncoupled from cell division. EMBO 5, 3563-3570. 17. Korn, L.J., Siebel, C.W., McCormick F. & R.A. Roth (1987). Ras p21 as a Potential Mediator of Insulin Action in Xenopus Oocytes. Science 236 840-843. 18. 侯叔珪，碩士論文，鯉魚c-myc基因結構分析輿表現之研究。 19. 王舜璋，碩士論文，鯉魚ubiquitin基因結構輿表現之研究。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75847 | - |
| dc.description.abstract | 為欲瞭解鯉魚卵細胞發育過程中之基因表現情形。本論文首先建立了卵原細胞期與高級卵黃期之鯉魚卵巢cDNA基因庫。經由兩基因庫兩萬重組基因株之差異篩選（differential hybridization）找出表現不同的cDNA約20個。將其中五個cDNA( Cov7, 10, 23, 24, 33）轉入質體，然後再以之為探針，對不同發育時期之鯉魚卵巢進行RNA dot雜交反應，結果發現Cov7, 23, 24與33等四株基因在發育早期卵細胞有明顯的表現，而Cov10基因則在卵細胞發育的過程中均有明顯但不等程度的表現。將核酸定序結果在“DNA Star”基因庫中比對。結果發現0.5kb長之Cov10為ubiquitin基因，但1.4kb長之Cov7則尚不知為何基因。 | zh_TW |
| dc.description.abstract | In order to study the gene expression during oogenesis in carp, two λgt10 cDNA libraries were constructed from carp ovaries of oogenium and tertiary yolk stages, respectively. Through differential hybridization of twenty thousand recombinant phages, twenty cDNA clones were obtained. Five of them, Cov7,10,23,24 and 33, were subcloned into plasmids and used as probes to study their expression during oogenesis. RNA dot analysis indicates that Cov7,23,24,33 are slightly expressed in the oocyte of young developmental stage and Cov10 is highly but differentially expressed throughout the oogenesis. After computer search of nucleotide sequences in the “DNA Star” gene bank, the 0.5 kb Cov10 is the gene encoding ubiquitin and a fuse protein. None of the reported genes is corresponding to the 1.4 kb Cov7. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:15:52Z (GMT). No. of bitstreams: 0 Previous issue date: 1991 | en |
| dc.description.tableofcontents | 中文摘要. . . . . . . . . . . . . .1 英文摘要. . . . . . . . . . . . . .2 緒言. . . . . . . . . . . . . .3 材料與方法. . . . . . . . . . . . . .7 實驗方法 cDNA Library的建立. . . . . . . . . . . . . .7 差異篩選. . . . . . . . . . . . . .11 嗜菌體的培養與其DNA之抽取. . . . . . . . . . . . . .12 Competent Cells的製備和質體轉入. . . . . . . . . . . . . .14 質體的製備. . . . . . . . . . . . . .14 Agarose gel electrophoresis and electroelution . . . . . . . . . 15 Nick Translation . . . . . . . . . . . . . 16 核酸定序. . . . . . . . . . . . . 17 RNA電泳. . . . . . . . . . . . . 18 RNA dot . . . . . . . . . . . . . 18 北方點墨法. . . . . . . . . . . . . 19 Erase a base system . . . . . . . . . . . . . 19 試劑. . . . . . . . . . . . . 20 結果. . . . . . . . . . . . . 27 討論. . . . . . . . . . . . . 31 參考資料. . . . . . . . . . . . . 35 | |
| dc.language.iso | zh-TW | |
| dc.title | 鯉魚卵細胞發育過程中基因表現變化之研究 | zh_TW |
| dc.title | Differential gene expression during oogenesis of carp | en |
| dc.date.schoolyear | 79-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 52 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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