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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Chao-His Huang | en |
| dc.contributor.author | 黃昭熹 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:15:49Z | - |
| dc.date.available | 2021-07-01T08:15:49Z | - |
| dc.date.issued | 1991 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75841 | - |
| dc.description.abstract | 利用小白雌鼠子宮分泌液(uterine fluid)免疫同系雌鼠，以酵素免疫分析法(ELISA)偵測，顯示可誘發雌鼠的自體免疫。由西方墨點法(Western blotting)知引起免疫反應的自體抗原其分子量為28kd，我們並將它命名為子宮液自體抗原(Uterine fluid autoantigen)，簡稱UFA。使用Sephadex G-100膠體過濾法和Reverse-phase-HPLC，我們將UFA分離。進一步的分析，發現UFA是一個近中性(PI約為7.6)的醣蛋白。其醣基約占醣蛋白重量的13%，而去醣後的UFA分子量降為23.5kd。我們並決定了UFA的氨基酸組成和N端的前十個氨基酸序到，而有關 UFA的完整一級結構及其所具的生理功能則尚需要進一步的研究才能知曉。 | zh_TW |
| dc.description.abstract | The female mice (8 wks) were autoimmunized with crude uterine fluid. The autoantisera were used for immunochemical detection of autoantigen(s) in uterine fluid. We found the autoantisera recognized a 28kd protein in uterine fluid and we named this protein uterine fluid autoantigen(UFA). We purified UFA through a series of procedures including Sephadex G-100 chromatography and Reverse-phase HPLC on a chemeco 5ODS-H column. UFA was shown to be a glycoprotein with isoelectric point of 7.6. The carbohydrate moiety constituted about 13% of total UFA mass. The protein core of UFA was estimated to be 23.5kd when glycoprotein was deglycosylated with TFMS. Moreover, we also determined the amino acid composition of UFA and the first ten amino acid sequences of its N-terminal region. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:15:49Z (GMT). No. of bitstreams: 0 Previous issue date: 1991 | en |
| dc.description.tableofcontents | 中文摘要. . . . . . . . . . . . . . . . . 1 英文摘要. . . . . . . . . . . . . . . . .2 一、緒言. . . . . . . . . . . . . . . . . 3 二、材料、藥品、儀器. . . . . . . . . . . . . . . . . 6 三、實驗方法. . . . . . . . . . . . . . . . . 7 (一)、子宮液的製備. . . . . . . . . . . . . . . . .7 (二)、自體免疫血清的製備. . . . . . . . . . . . . . . . . 7 (三)、酵素連結免疫吸著劑分析法(ELISA) . . . . . . . . . 8 (四)、電泳分析. . . . . . . . . . . . . . . . . 10 (五)、Coomassie Blue Staining. . . . . . . . . . . . . . . . .11 (六)、西方墨點法(Western blotting). . . .. . . . . .11 (七)、蛋白質的純化. . . . . . . . . . . . . . . . . 12 A、Sephadex G-100膠體管柱. . . . . . . . . . . . . . . . . 12 B、Reversed-phase-HPLC. . . . . . . . . . . . . . . . . 13 (八)、等電焦集法(Isoelectrofocusing，IEF). . . . . . . . 14 (九)、PAS染色法(Periodic Acid Schiff'’s reagent stain). . . 14 (十)、醣基定量法(L-cysteine sulphuric acid assay). . . . 15 (十一)、化學去醣法(Chemical Deglycosylation) . . . . .15 (十二)、氨基酸組成分析和N-terminal sequence的定序. . .16 (十三)、蛋白質的定量. . . . . . . . . . . . . . 16 四、實驗結果與討論. . . . . . . . . . . . . .17 (一)、子宮液的製備. . . . . . . . . . . . . . 17 (二)、自體抗原的發現. . . . . . . . . . . . . . 17 (1) ELISA . . . . . . . . . . . . . 18 (2) Western blotting . . . . . . . . . . . . 18 (三)、自體抗原(UFA)的純化. . . . . . . . . . . .19 (四)、UFA是一個醣蛋白. . . . . . . . . . . .20 (五)、化學去醣反應. . . . . . . . . . . .20 (六)、分子量及含醣量的估算. . . . . . . . . . . .21 (七)、等電點(PI)的估算. . . . . . . . . . . .21 (八)、氨基酸的組成分析和N-terminal sequence的決定. . 22 五、展望. . . . . . . . . . . . 24 六、圖表. . . . . . . . . . . .26 七、參考資料. . . . . . . . . . . . 40 | |
| dc.language.iso | zh-TW | |
| dc.title | 小白鼠子宮分泌液自體抗原的純化輿特性鑑定 | zh_TW |
| dc.title | Isolation and Characterization of Mouse Uterine Fluid Autoantigen(UFA) | en |
| dc.date.schoolyear | 79-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 47 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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