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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 鍾郁文 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:15:22Z | - |
| dc.date.available | 2021-07-01T08:15:22Z | - |
| dc.date.issued | 1991 | |
| dc.identifier.citation | Allan, D., P. Thomas & R. H. Michell (1978) “Rapid trans-bilayer diffusion of 1,2-diacylglycerol and its relevance to control of membrane curvature.” Nature 276: 289-290.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75785 | - |
| dc.description.abstract | 經75% ammonium sulfate沉澱，初步純化的Edwardsiella tarda之外毒素，會引起人類紅血球的溶血。將此外毒素通入G-25 Sephadex Column去除鹽類，可分出5個蛋白質群(G25-1?5)。其中G25-1含有可以水解phosphotidylcholine (PC)和sphingo-myelin (SM)之磷脂?C；G25-2則含有會水解PC、SM和phos-photidylethanolamide 2(PE)之磷脂?。將G25-2再通入DEAE-Sephacel Anion Exchange Column可得6個蛋白質群(DEAE1?6)。其中DEAE2含有對PE具種別性之磷脂?A，DEAE5能水解PC和SM，DEAE6則含有對PE具種別性之磷脂?C。DEAE5進一步以Electrofocusing Column分離，可得5個蛋白質群(E1?5)。E3、E5分別含有對PC具種別性之磷脂?A、C。此乃首次發現E.tarda之外毒素具磷脂?的活性。 | zh_TW |
| dc.description.abstract | The crude exotoxin of Edwardsiella tarda, obtained by 75% ammonium sulfate precipitation , caused hemolysis of human erythrocytes. The exotoxin was then desalted by G-25 Sephadex Column and recovered five peaks. Two major peaks (G25-1,2) had lipase activities. G25-1 Contains phospholipase C which hydrolyzed phosphotidylcholine (PC) and sphingomyelin (SM). G25-2 hydrolyzed PC, SM and phosphotidyleth-anolamide(PE). G25-2 was then fractionated by DEAE-Sephacel column chromatography. Three different fractions showed various phospholipase activities. DEAE 2 contained basic PE- specific phospholipase A. DEAE 5 hydrolyzed sphingomyelin and PC; DEAE 6 contained acidic phospholipase C which hydrolyzed PE . DEAE5 was further seperated by Electrofocusing Column . Two fractions recovered lipase activities. E3 contained PC-specific phospholipase A; while E5 had PC-specific phospholipase C . This shows that the exotoxin of E.tarda had various kinds of phospholipaseactivities. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:15:22Z (GMT). No. of bitstreams: 0 Previous issue date: 1991 | en |
| dc.description.tableofcontents | 圖目錄. . . . . . . . . . . . . . . . . . . . . . . . . . . ii 摘要. . . . . . . . . . . . . . . . . . . . . . . . . . . . iii 一、緒論. . . . . . . . . . . . . . . . . . . . . . . . . . 1 二、材料與方法. . . . . . . . . . . . . . . . . . . . . . . 4 三、結果. . . . . . . . . . . . . . . . . . . . . . . . . . 15 四、討論. . . . . . . . . . . . . . . . . . . . . . . . . . 42 五、參考文獻. . . . . . . . . . . . . . . . . . . . . . . . 47 六、英文摘要. . . . . . . . . . . . . . . . . . . . . . . . 54 七、謝辭. . . . . . . . . . . . . . . . . . . . . . . . . . 55 | |
| dc.language.iso | zh-TW | |
| dc.title | Edwardsiella tarda外毒素之磷脂?活性 | zh_TW |
| dc.title | Identification of Phospholipase Activities in the Exotoxin of Edwardsiella tarda | en |
| dc.date.schoolyear | 79-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 55 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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