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標題: | 貓延腦吻端縫合核有關呼吸方面單一神經細胞的探討 Single Unit Study of Respiratory-related Neuron in Rostral Medullary Raphe Nuclei of Cats |
作者: | 簡佩玉 |
出版年 : | 1991 |
學位: | 碩士 |
摘要: | 本實驗目的是想在延腦吻端縫合核(RMRN)找尋與呼吸有關的單一神經細胞,並探討其對二氧化碳濃度提高的反應及與其他功能細胞間的關聯性。以貓為材料,去大腦、肌肉麻痺、兩側迷走神經切斷後,以人工呼吸機器維持其正常通氣量(ventilation)。分離並記錄膈神經。記錄血壓。然後,將動物維持在高氧和二氧化碳濃度正常(hyperoxic normocapnia)的狀態下,以金屬微電極記錄單一神經細胞。當記錄到確認為細胞本體所發出的動作電位時,將二氧化碳濃度提高(hypercapnia),以測試該神經細胞的反應。然後,由靜脈注入腎上腺素,使血壓上升。最後再以止血鉗夾住體表皮膚,觀察其放電率的變化。 在所記錄到124個神經細胞中,有41個屬於與呼吸有關的神經細胞,其類型有I,E,IE,EI,T-EI及其他等,但最常見的是EI和T-EI,而41個中有14個的放電率會在二氧化碳濃度增加而降低(p<0.01)。57個神經細胞中,有15個會在血壓上升時增高其放電率,另13個則下降,其餘沒有反應。其中6個屬於與呼吸有關的神經細胞。40個中有11個對傷害性刺激會增加其放電率,另3個則降低,其中有3個屬於與呼吸有關的神經細胞。觀察記錄位置,三群細胞間並無明顯分界。 這些結果顯示,在RMRN確實含有一些與呼吸有關的神經細胞。雖然與呼吸有關的、受血壓影響的、與痛覺傳遞有關的神經細胞大部分屬三種不同細胞群,但卻互相混在一起。 The purpose of the present study was to characterize respiratory-related unit activities in the rostral medullary raphe nuclei (RMRN). Cat was decerebrated, paralyzed, vagotomized and ventilated. Under hyperoxia and normocapnia, the phrenic (C5) was isolated and its activity was monitored. Single unit activity of the RMRN was recorded with metal microelectrode. Phrenic activity triggered average of the spontaneous discharge of the unit was used to characterize respiratory-related neuron. All units were challenged with hypercapnic test. Some of the units were also tested for cardiovascular or nociceptive functions. These were tested by elevation of the blood pressure with intravenous infusion of adrenalin or pinching various parts of the body respectively. In a total of 124 units, 41 neurons showed respiratory-related activities which included inspiratory (I), expiratory (E), inspiratory-expiratory (IE), expiratory-inspiratory (EI), tonic-inspiratory-expiratory (T-IE) and tonic-expiratory-inspiratory (T-EI) patterns. Among these, respiratory-related neurons showed highest probability of EI and T-EI patterns. During hypercapnic test, a large portion of either respiratory or non-respiratory-related neurons showed decrease of discharge rate. Fifty-seven of 124 units were tested for their responses to blood pressure elevation. Among them, 28 were cardiovascular-related. Six of them were also respiratory-related. Fourteen of 40 neurons responded to nociceptive stimulation and 3 of them were demonstrated respiratory-related activity. None of these three functional groups of neurons had topographic organization in RMRN. In other words, neurons in these three groups were intermingled with each other. The results suggest that there are respiratory-related neurons within the RMRN. Portion of the respiratory-related neurons are modulated by other stimulations such as blood pressure change and nociception. These neurons are mixed with cardiovascular-related and nociceptive-related neurons in the RMRN with neither significant overlap nor segregation. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75782 |
全文授權: | 未授權 |
顯示於系所單位: | 動物學研究所 |
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