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標題: | 低溫逆境對耐寒和不耐寒植物之外部形態、內部微細構造和蛋白質合成的比較 The Comparison of Chilling Stress on Morphological, Ultrastructure and Protein Synthesis in Chilling Sensitive (Mung Bean) and Chilling Insensitive (Pea) Plants |
作者: | 馬淑芳 |
關鍵字: | chilling stress,pea,mungbean,chilling injury (CI),chloroplast ultrastructural changes,cold acclimation,SDS-PAGE,fluorography, |
出版年 : | 1988 |
學位: | 碩士 |
摘要: | 中文摘要 低溫敏感性植物--綠豆(Vigna radiate.L)和低溫不敏感性植物--豌豆(Pisum sativum L. cv. Taichung No 9)兩種植物,在 28℃光照下生長7-10天後,移到4℃光照下,兩者對低溫造成的傷害程度有明顯的不同。綠豆幼苗經3小時低溫處理,其草質莖由於脫水而下垂,24小時後葉片也嚴重脫水而捲曲,且光照下所造成的傷害比黑暗中更嚴重。2天的低溫處理已使綠豆幼苗達到永久凋萎的程度,終至死亡,但豌豆幼苗在4℃低溫處理,不論經歷多久都不會引起脫水而導致死亡,只會引起植株生長的抑制作用。 由TEM觀察葉綠體的微細構造,發現綠豆葉綠體在光照下4℃ 3小時就開始有澱粉粒累積,但24小時後累積的澱粉粒遽減,使得類囊膜之間的距離緊縮。在黑暗下,葉綠體內澱粉粒的變化與光照下相類似,但較不顯著;類囊膜的排列也較鬆弛。豌豆的葉綠體在4℃光照下,在24小時內澱粉粒的累積雖不像綠豆幼苗那麼顯著,但經長期的低溫處理,葉綠體會膨脹成橢圓,葉綠餅排列傾斜,類囊膜逐漸鬆弛,尾端形成小囊泡;甚至崩解。 低溫逆境可誘導植物幼苗葉片或葉綠體內許多新蛋白質的合成,也會抑制許多正常蛋白質的合成。其中有一群19KD的蛋白質,卻只在低溫處理過的豌豆或經冷馴化過程中的綠豆中發現,它是屬於類囊膜系的蛋白質。 4℃的低溫,顯然己使植物體內ACC轉變成乙烯的步驟受到嚴重的傷害,因此在低溫下,綠豆和豌豆幼苗的乙烯釋出量都很低,甚至移到28℃ 2小時後,其釋出的量也二不會增加。 英文摘要 There are different kinds of chilling injury between mung bean (chilling sensitive plant) and pea (chilling insensitive plant) It appears slightly wilting in mung bean stem after it being chilled for 3 hrs; and chilled for 12 hrs, leaves are also wilting. Plants are dead for chilled 2 days. Plants chilled in the light are greater injured than in the dark. The growth of pea is retarded no matter how long chilled it is. There are many starch granules accumulated in these chloroplasts of mung bean for chilled 24 hrs. but that of peas is not apparently. For chilled longer periods, they are manifested by distortion and swelling of thylakoids, the formation of small vesicles, or the rupture of chloroplasts. Exposure of pea and mung bean at 4℃ results in the appearance of several new proteins which is not present In plants grown at 28℃. The fluorograms of the SDS-PAGE of 35S-methionine labelled shows that 19 KD protein was located in the thylakoid membrane of the chloroplasts of pea, and cannot find on mung bean unless which has been acclimated at 8℃ for 1 day. After chilled at 4℃, the step of ACC converting into ethylene is apparantly damaged in pea and mung bean. So the increased ethylene production from chilled plants is very little. Recovered at 28℃ for 2 hrs, the step connot be stimulated. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75730 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
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