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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Ching-Jin Chang | en |
| dc.contributor.author | 張靜仁 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:14:56Z | - |
| dc.date.available | 2021-07-01T08:14:56Z | - |
| dc.date.issued | 1989 | |
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(1988) Hepatocyte-stimulating factor, -2interferon, and interleukin-1 enhance expression of the rat .1-acid glycoprotein gene via a distal upstream regulatory region. Mol. Cell Biol. 8:42-51. 6. Baumann, H., Isseroff, H., Latimer, J.J., and Jahreis, G.P. (1988) Phorbol ester modulates interleukin6- and interleukinl-regulated expression of acute phase plasma proteins in hepatoma cells. J. Biol. Chem. 263:17390-17396. 7. Liu, H.M., Takagaki, and Schmid, K. (1988) In vitro Nerve-growth-promoting activity of human plasma .1-acid glycoprotein. J. Neuroscience Res. 20:64-72. 8. Lee, S.C., Chang, C.J., Lee, Y.M., Lei, H.Y., Lai, M.Y., and Chen, D.S. (1989) Molecular cloning of cDNAs corresponding to two genes of .1-acid glycoprotein and characterization of two alleles of AGP-1 in the mouse DNA, 8:245-251. 9. Baumann, H., Held, W.A., and Berger, F.C. (1984) The acute phase response of mouse liver. J. Biol. Chem. 259:566-573. 10. Dente, L., Ciliberto, G. and Cortese, R. 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(1987) Methods in Enzymology. Volume 152. 13. Practical aspects of preparing phage and plasmid DNA: growth, Maintenance, and storage of Bacteria and Bacteriophage. Academic press, INC. 30. Hames, B.D., and Higgins, S.J. (1984) Transcription and translation - A practical approach. chapter 1 Expression of exogenous DNA in Mammalian cells. 31. Graham, F.L., and Van Der Eb, A.J. (1973) A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456-467. 32. Gorman, C.M., Moffat, L.F., and Howard, B.H. (1982) Recombinant Genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell Biol. 2:1044-1051. 33.Berger, S.L., and Kimmel, A.R. (1987) Methods in enzymology. Volume 152. 72: Identification of regulatory elements of cloned genes with functional assay. Academic Press, INC. 34. Melton, D.A., Krieg, P.A., Rebagliati, M.R., Maniatis, T., Zinn, K., and Green, M.R. (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucl. Acids. Res. 12:7035-7056. 35. Cooper, R. Eckley, D.M. and Papaconstantinou, J. (1987) Nucleotide sequence of the mouse .1-acid glycoprotein gene 1. Biochemistry 26:5244-5250. 36. Cullen, B.R., Lomedico, P.T., and Ju, G. (1984) Transcriptional interference in avian retrovirues - implicatious for the promoter insertion model of leukalmogenesis. Nature 307:241-245. 37. Proudfoot, N.J. (1986) Transcriptional interference and termination between duplicated .-globin gene constructs suggests a novel mechanism for gene regulation. Nature 322:562-565. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75725 | - |
| dc.description.abstract | αl-acid glycoprotein是一種主要的急性狀態反應劑．我們己經從小老鼠肝臟選殖到兩種αl- AGP的CDNA，命名為AGP-1和AGP- 2．其中，AGP-l具有兩種不同的基因型態，在不同品系的老鼠中使限制的認知位置改變，這所謂的AGP-1A和AGP-1B的CDNA已經從(B10X AKR/J) F1的CDNA基因庫中篩選出，並完全定序，發現在編譯區一個核?酸的改變，使AGP-1A和AGP-1B所帶電荷不等，而造成在電泳中移動速率不一致． 以AGP-1 CDNA為探針篩選C57BL/6的基因，共找到4個可以雜化的純系，加以分析後，發覺其中包含三個AGP基因，AGP-1, AGP-2，和AGP-3．經過限制?圖的分析和部分的核酸定序，已分離出三個基因的?動子區．利用引子延伸的技術，我們決定了AGP-1轉錄的起始點，並且知道在正常肝臟狀況下，AGP-1能有效率的表現，發炎時，能大量增加其轉錄量，AGP-2則不能． 將AGP-1和AGP-2的?動子區分離出，進行DNA轉染(Transfection)實驗，藉此研究糖皮素(glucocorticoid)及巨噬細胞分必因數對AGP基因的調控，和AGP-1邊與AGP-2轉錄的差別． | zh_TW |
| dc.description.abstract | α1-acid glycoprotein is a major acute phase reactant. We have cloned two mouse α1-Agp cDNAs, termed Agp-1 and Agp-2. Two alleles of Agp-1 have also been identified from inbred mice by restriction site polymorphism. cDNA of both Agp-1A and Agp-1B have been cloned from (Bl0×AKR/J)F1 mouse and sequenced. A single nucleotide substitution in the coding sequence of Agp-1 is probably responsible for the observed difference in electrophoretic mobilities of Agp-1A and Agp-1B. Four λ genomic clones containing Agp-1, Agp-2 and Agp-3 have been isolated from C57BL/6 genomic library. Restriction mapping and partial sequencing have been employed for isolation of promoter sequences. Primer extension experiment has been used to determine the transcription initiation site; only Agp-1 is efficiently expressed in normal liver and is induced substantially during inflammation. Promoters of Agp-1 and Agp-2 were used for functional assay by transfection. We intend to study the gene regulation, by glucocorticoid and macrophage screted factors, and the molecular basis of differential transcription of Agp-1 and Agp-2. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:56Z (GMT). No. of bitstreams: 0 Previous issue date: 1989 | en |
| dc.description.tableofcontents | 一、摘要• • • • • • • • • • • • • • • 1 二、緒言• • • • • • • • • • • • • • • 3 三、材料與方法 • • • • • • • • •6 1. Preparation of RNA 2. Construction of CDNA library 3. Screening library with nucleic acid probes 4. Isolation of phage DNA: liquid lysate method 5. DNA sequencing 6. Primer extension 7. Plasmid construction 8. Isolation of plasmid DNA: small scale and large scale 9. Transfection 10. CAT Assay 四、結果 • • • • • • • • • • • • • • • • • • • • • • •20 五、討論• • • • • • • • • • • • • • • • • • • • • • • 24 六、附圖 • • • • • • • • • • • • • • • • • • • • • • •28 七、參考文獻• • • • • • • • • • • • • • • • • • • • • 40 | |
| dc.language.iso | zh-TW | |
| dc.title | 老鼠αl-酸性醣蛋白基因的結構和表現 | zh_TW |
| dc.title | Structure and Expression of Mouse αl-acid Glycoprotein Genes | en |
| dc.date.schoolyear | 77-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 48 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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