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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 林相佑 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:14:56Z | - |
| dc.date.available | 2021-07-01T08:14:56Z | - |
| dc.date.issued | 1989 | |
| dc.identifier.citation | Agellon, L.B. and Chen, T.T. (1986) Rainbow trout growth hormone: Molecular cloning of cDNA and expression in Escherichia coli. DNA 5, 463-471.
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(1970) Primary lactogenic hormone XXX: Primary structure of the sheep hormone. Arch. Biochem. Biophys. 141, 705-737. Linzer, D.I.H. and Talamante, F. (1985) Nucleotide sequence of mouse prolactin and growth hormone: zmRNAs and expression of these mRNAs during pregnancy. J. Biol. Chem. 17, 9574-9579. Martial, J.M., Hallewell, R.A., Baxter, J.D. and Goodman, H.M. (1979) Human growth hormone: Complementary DNA cloning and expression in bacteria. Science 205, 602-607. Maxam, A.M. and Gilbert, W. (1977) A new method for sequencing DNA. Proc. Natl. Acad. Sci. USA 74, 560-564. Mercier, L., Rentier-Delrue, F., Swennen, D., Lion, M., Le Goff, P., Prunet, P. and Martial, J.A. (1989) Rainbow trout prolactin cDNA cloning in Escherichia coli. DNA 8, 119-125. Miller, W.L., Coit, D., Baxter, J.D. and Martial, J.A. (1981) Cloning of bovine prolactin cDNA and evolutionary implications of its sequences. DNA 1, 37-50. Miyazima, K., Kawauchi, H. and Peter, R.I. (1986) Isolation of carp prolactin and growth hormone. In “Proceedings of the Spring Meeting of the Japanese Society of Scientific Fisheries” (Fujitani, S. Ed.) p. 71. Nicoll, C.S. (1980) Ontogeny and evolution of prolactin’s function. Fed. Proc. 39, 2563-2566. Pan, F.M. and Chang, W.C. (1989) Cloning and sequencing of bullfrog growth hormone complementary DNA. Biochim. Biophys. Acta 950, 238-242. Prunet, P. and Hondebine, L.M. (1983) Purification and biological characterization of chinook salmon prolactin. Gen. Comp. Endocrinol. 53, 49-57. Rentier-Delrue, F., Swennen, D., Mercier, L., Lion, N., Benrubi, O. and Martial, J.A. (1989) Molecular cloning and characterization of two forms of trout growth hormone cDNA: Expression and secretion of tGH-II by Escherichia coli. DNA 8, 119-127. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467. 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(1984) Application of recombinant DNA echnologies to studies on chicken growth hormone. J. Expt. ool. 232, 465-473. ker, J.L., King, D.S., Nishioka, R.S., Shirahata, K., amaguchi, K. and Bern, H.A. (1985) Isolation and partial haracterization of a pair of prolactins released in vitro by he pituitary of a cichlid fish, Oreochromis mossambicus. roc. Natl. Acad. Sci. USA 82, 7490-7494. Dkawa, M., Muramoto, K. and Kawauchi, H. (1985) Primary tructure of fin whale prolactin. Int. J. Peptide Proteins. 25, 442-448. hiki, M., Tanaka, M., Masuda, N., Yamkawa, M., Yoneda, Y. and akashima, K. (1988) cDNA cloning and primary structure of allow tail (Seriola quinqueradiata) pregrowth hormone. Gen. Comp. Endocrinol. 70, 401-406. elaar Bonga, S.E., van der Meij, J.C.A. and Plik, G. (1984) rolactin and acid stress in the teleost Oreochromis (formerly arotherodon) mossambicus. Gen. Comp. Endocrinol. 55, 323-42. guchi, K., Yasuda, A., Kishida, M., Hirano, T., Sano, H. and twauchi, H. (1987) Primary structure of eel (Anguilla tponica) growth hormone. Gen. Comp. Endocrinol. 66, 447-453. La, A., Itoh, H. and Kawauchi, H. (1986) Primary structure l chum salmon prolactins: occurence of highly conserved egions. Arch. Bioch. Biophys. 244, 528-541. La, A., Miyazima, K.I., Kawauchi, H., Peter, R.E., Lin, H.R., imaguchi, K. and Sano, H. (1987) Primary structure of common .rp prolactins. Gen. Comp. Endocrinol. 66, 280-290. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75724 | - |
| dc.description.abstract | 本研究主要目的在純化鼠類子宮分泌的Lactotransferrin，並製備專一性高的Anti-Lactotransferrin抗體，作為酵素免疫分析(ELISA)及西方墨點法(Western blotting)方法使用。 酵素免疫分析法的結果知 (l) 血清中，雌雄老鼠都有但雌的略高於雄的，但其量每ug血清中約為1ng的Lactotransferrin。 (2) 生殖器官中，雄老鼠只有貯精囊有且量極高，睾丸、副睾、前攝護腺都沒有，雌老鼠卵巢、陰道、乳線存在有，子宮則未測試到。(3)其他組織腦、肝、心、肺、胰、腎、脾、胸線、十二指腸中，雌雄老鼠二者都存在的有肝、脾、腎，雌的含量略高於雄的含量。另外，雌的胸腺有而雄的沒有。雌的腦中有時可以測到。西方墨點法(Western blotting)的結果，除腦外其餘組織均可測試到有一 65K的蛋白質會和Anti-Lactotransferrin抗體反應。有人報告此65K可能是Lactotransferrin的Low Molecular weight型式，而有待進一步證實。 | zh_TW |
| dc.description.abstract | Among the hormones of fish pituitary gland, prolactin (PRL) plays an important role in osmoregulation and freshwater adaption. For the purpose of undersdanding the amino acid sequence of grass carp (Ctenopharyngodon idellus) PRL, a cDNA library was constructed from the pituitary gland using λ gt10 as cloning vector. The carp PRL cDNA, which was obtained by screening the carp pituitary cDNA library with 32-mer synthetic probe, was labeled by nick translation and used as probes to screen the cDNA library for grass carp PRL. Four positive clones was obtained and the longest cDNA was subjected to nucleotide sequence analysis. It is shown that the cDNA is 633 bp in length and contains a coding region of 273 bp and a non-coding region of 357 bp. While the deduced amino acid sequence of this 91 amino acid residue polypeptide shows high extent of homology (94.5%) with the carboxyl terminal portion of carp PRL( i.e. amino acid residue 97 to 187), it is 64% similar to salmon PRL and only 30% homolgous to human PRL. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:56Z (GMT). No. of bitstreams: 0 Previous issue date: 1989 | en |
| dc.description.tableofcontents | 摘要- - - - - - - - - - - -1 英文摘要- - - - - - - - - - - - -2 緒言- - - - - - - - - - - - - - -3 材料與方法- - - - - - - - - - 7 實驗動物- - - - - - - - - - - - 7 E.coli和vector- - - - - - - -7 藥品- - - - - - - - - - - - - - - - 7 RNA製備- - - - - - - - - - - -8 poly(A)+ mRNA的分離- - - - - - - - - - - 10 雙股cDNA的製備與cDNA基因庫之建立- - - - - - - - - - -11 5'端標示合成DNA探針- - - - - - - - - - 15 以Nick translation製備DNA探針- - - - - - - - - - -16 λgt10 cDNA library的選殖- - - - - - - - - 17 噬菌體的培養- - - - - - - - - - - - - - - - - - -20 噬菌體DNA的製備- - - - - - - - - - - - - -22 Competent cell的製備- - - - - - - - - - - - -25 菌體轉型- - - - - - - - - - - - - - - - - - - -26 Agarose gel eletrophoresis and- - - - - - - - - - - - -27 eletroelution- - - - - - - - - - - - - 28 核酸定序- - - - - - - - - - - - - 28 Formaldehyde gel for electrophoretic- - - - - - - - - - -31 separation of RNA- - - - - - - - - - - Spin column chromatography- - - - - - - - - - -32 結果- - - - - - - - - - -34 討論- - - - - - - - - - -45 參考資料- - - - - - - - - - -48 | |
| dc.language.iso | zh-TW | |
| dc.title | 草魚泌乳激素cDNA的選殖與核酸定序 | zh_TW |
| dc.title | cDNA Cloning and Sequencing of Grass Carp Prolation | en |
| dc.date.schoolyear | 77-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 55 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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