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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 餘榮熾 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:14:35Z | - |
| dc.date.available | 2021-07-01T08:14:35Z | - |
| dc.date.issued | 1987 | |
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Structure and location of the O-glyco-sidic carbohydrate units of human chorionic gonadotropin. J. Biol. Chem. 254, 7909-7914. Kessler, M.J., Reddy, M.S., Shah, R.H., and Bahl, O. P. (1979). Structure of N-glycosidic carbohydrate units of human chorionic gonadotropin. J. Biol. Chem. 254, 7901-7908. Licht, P.., Famer, S.W., Bona Gallo, A., and Papkoff, H. (1979). Pituitary gonadotropins in snakes. Gen. Comp. Endocrinol. 39, 34-52. Licht, P., Famer, S.W., and Papkoff, H. (1976). Further studies on the chemical nature of reptilian gonadotropins: FSH and LH in the American alligator and green sea turtle. Biol. Reprod. 14, 222-232. Licht, P., Famer, S.W., and Papkoff, H. (1978). Biological activity of hybrid combinations of ovine and sea turtle LH subunits. Gen. Comp. Endocrinol. 35, 289-29 4. Licht, P., and Papkoff, H. (1974a). Separation of two distinct gonadotropins from the pituitary gland of the bullfrog Rana catesbeiana. Endocrinology 94, 1587-1594. Licht, P., and Papkoff, H. (1974b). Separation of two distinct gonadotropins from the pituitary gland of the snapping turtle (Chelydra serpentina). Gen. Comp. Endocrinol. 22, 218-237. Licht, P., Papkoff, H., Farmer, SW., Muller, C.H., Tsui, H.W., and Crews, D. (1977). Evolution of gonadotropin structure and function. Recent Progr. Hormone Res. 33, 169-248. Lo, T.B., Huang, F.L., arid Chang, G.D (1981). .Separation of subunits of pike eel gonadotropin by hydrophobic interaction chromatography. J. Chromatography 215, 229-233. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951). Protein measurement with phenol reagent. J. Biol. Chem. 193, 265-275. Menn, F.L., and Burzawa-Gerard, E. (1985). Effect of carp gonadotropin (cGTH) and a fraction unabsorbed on concanavalin A-sepharose obtained from cGTH on vitellogenesis in the hypophysectomized marine teleost Gobius niger. Gen. Comp. Endoctinol. 57, 23-36. Mueller, J.M., Pierce, J.G., Davoll, H., and Vigneaud, V.D. (1951). The oxidation of oxytocin with performic acid. J. Biol. Chem. 191, 309-313. Ng, T.B., and Idler, D.R. (1979). Studies on two types of gonadotropins from both American plaice and winter flounder pituitaries. Gen. Comp. Endocrinol. 38, 410-420. Papkoff, H. (1974). Chemical and biological properties of the subunits of pregnant mare serum gonadotropin. Biochem. Biophys. Res. Commun. 58, 397-404. Papkoff, H., Farmer, S.w., and Licht, p. (1976). Isolation and characterization of follicle-stimulating hormone and luteinizing hormone and its subunits from snapping turtle (Chelydra serpentina) pituitaries. Endocrinology 98,767-777. Papkoff, H., Sairam, M.R., and Li, C.H. (1971). Amino acid sequence of the subunits of ovine pituitary interstitial cell-stimulating hormone. J. Amer. Chem. Soci. 93, 1531-1532. Papkoff, H., and Samy, T.S.A. (1967). Biochim. Biophys. Acta 147, 175. Pierce, J.G., and Parsons, T.F. (1981). Glycoprotein hormones: structure and function. Ann. Rev. Biochem. 50, 465-495. Rathnam, P., and Saxena, B.B. (1971). Subunits of luteinizing hormone from human pituitary glands. J. Biol. Chem. 246, 7087-7094. Reichert, L.E. Jr., and Ward, D.N. (1974). On the isolation and characterization of the alpha and beta subunits of human pituitary follicle-stimula-ting hormone. Endocrinology 94, 655-664. Sairam, M.R., and Bhargavi, G.N. (1985). A role for glycoprotein of the a subunit in transduction of biological signal in glycoprotein hormones. Science 229, 65-67. Smith, P.E. (1927). J. Am. Med. Assn. 88, 158. Stellman, S.L., Segaloff, A., and Mays, M.G. (1958). Preparation of highly active human gonadotropin and growth hormone. Arch. Biochem. Biophys. 78, 262-26 3. Swaminathan, N., and Bahl, O.P. (1970). Dissociation and recombination of the subunits of human chorionic gonadotropin. Biochem. Biophys. Res. Commun. 40, 422-427. Trinh, K.Y., Wang, N.C., Hew, C.L., and Crim, L.W. (1986). Molecular cloning and sequencing of salmon gonadotropin subunit. Eur. J. Biochem. 159, 619-624. Weber, K., and Osborn, M. (1969). The reliability of molecular weight determination by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244, 4406-4412. Williams, R.H. (1981). Textbook of endocrinology. W. B. Saunders Company. 劉若文，黃火鍊(1985)。鯉魚腦下腺性腺刺激素次單元之分離及特性研究。國立台灣大學動物學研究所碩士論文。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75674 | - |
| dc.description.abstract | 摘要 本實驗的目的是分離大頭鰱(Hypophthalmichthys nobilis; Bighead Carp)腦下腺性腺刺激素及次單元。 大頭鰱腦下腺粉末以40％乙醇、6％醋酸銨、pH5.1的溶液於4℃下萃取，再以乙醇(最後濃度80％)沈澱醣蛋白。所得之醣蛋白以Sephadex G-100分離，得到三個部分，其中第二部分(BH G2)具有最強的性腺刺激素活性。 本實驗發展以HPLC分離大頭鰱性腺刺激素次單元之系統。Column為Nucleosil 7μ C18之reverse phase column；溶劑系統包括溶劑A(水：氰甲烷：三氟醋酸＝77.5:22.5:0.07體積比)及溶劑B(水：氰甲烷：三氟醋酸＝68:32:0.07體積比)；滌流條件為溶劑B對溶劑A作非線性梯度的濃度增加，於30分鐘時，溶劑B達85％。BH G2在此系統中展開為五個部分，C1?C5；其中C1及C5為最主要部分。經過各次單元重組結合後之生物活性測定，氨基酸組成分析及N-末端部分氨基酸順序分析，我們認為C1,C2，C3為大頭鰱性腺刺激素的α次單元，C4及C5為β次單元。 α次單元(C1)之氨基酸組成中含高量的Asp(10.71%),Cys(9.41%)及val(9.09%)，而β%單元(CS)則含有高量的Glu(10.56%),Pro(9.54%),Asp(10.80%)，及Val(11.00%)。經由SDS-PAGE的鑑定，α次單元的分子量為17,400 daltons，β次單元為26,000daltons。 | zh_TW |
| dc.description.abstract | Abstract The present study was aimed to isolate the subunits of bighead carp (Hypophthalmichthys nobilis) gonadotropin and to characterize their chemical and biological properties. Bighead carp pituitary glands were extracted with 40% ethanol—6% ammonium acetate solution, pH5.l at 4°C, and precipitated with ethanol (final concentration 80%). The crude glycoprotein was then chromatographed by Sephadex G-l00. Three fractions were obtained, in which the second fractron (BH G2) possessed the highest biological GTH activity. In this study, isolation of subunits of bighead carp GTH was performed by high performance liquid chromatography (HPLC). Reverse phase column of Nucleosil 7μ Cl8 was used; the solvent system included solvent A (water: acetonitrile: trifluroacetic acid=77.5:22.5:0.07 v/v) and solvent B (water: acetonitrile: trifluroacetic acid=68:32:0.07 v/v); concentration of solvent B was eluted from 0 to 85% non-linearly within 30min. There were five fractions obtained from this HPLC system. They were designed as C1 to C5 according to their retention time, with C1 and C5 being the two major fractions. From the data of amino acid composition, N-terminal partial sequence and the biological GTH activity of recombinants, we inferred that C1, C2 and C3 belonged to the α subunit whereas C4 and C5 belonged to the β subunit. The amino acid composition of the α subunit (C1) was rich in Asp (10.71%), Cys(9.41%) and Val(9.09%), while the β subunit (C5) was rich in Glu(10.86%), Pro(9.84%), Asp(10.80%)and Val(11.00%). From the result of SDS-PAGE, we estimated that the molecular weights of α and β subunits were 17,400 daltons and 26,000 daltons, respectively. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:35Z (GMT). No. of bitstreams: 0 Previous issue date: 1987 | en |
| dc.description.tableofcontents | 目錄 摘要..................1 緒言..................3 實驗材料與方法..................7 一、實驗動物..................7 二、化學藥品..................7 三、性腺刺激素之純化..................8 四、性腺刺激素生物活性測定..................9 五、利用HPLC分離性腺刺激素次單元..................13 六、性腺刺激素次單元之相互結合..................13 七、SDS-Polyacrylamide gel electrophoresis..................14 八、氨基酸組成分析..................14 九、N-末端部分氨基酸順序分析..................15 結果..................16 一、大頭鰱性腺刺激素之純化..................16 二、性腺刺激素次單元之分離..................16 三、各次單元及次單元結合體之生物活性..................20 四、SDS-Polyacrylamide gel electrophoresis..................20 五、氨基酸組成及N-末端部分氨基酸順序 .................24 討論.................27 附記：DEAE-cellulose對大頭鰱性腺刺激素的分離效應...........31 英文摘要.................35 參考文獻.................37 | |
| dc.language.iso | zh-TW | |
| dc.title | 大頭鰱性腺刺激素次單元之性狀 | zh_TW |
| dc.title | The Properties of the Gonadotropin Subunits of Bighead Carp | en |
| dc.date.schoolyear | 76-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 50 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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