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DC 欄位 | 值 | 語言 |
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dc.contributor.author | 陳英男 | zh_TW |
dc.date.accessioned | 2021-07-01T08:14:34Z | - |
dc.date.available | 2021-07-01T08:14:34Z | - |
dc.date.issued | 1988 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75670 | - |
dc.description.abstract | 本論文之目的在於探討魚類卵細胞在最後成熟(Final maturation)過程中參與滲透壓調節之成份變化,以作為進一步瞭解其控制機制的基礎。在本實驗中將香魚卵細胞的發育過程分為五個階段,分別是卵黃先質合成期(Vitellogenesis)時之初期卵黃期(Primary Yolk Globule Stage; YGI)和卵黃第三期(Tertiary Yolk Globule Stage; YG3),最後成熟過程中之卵核移動期(Germinal Vesicle Migration Stage; GVM)和排卵產期(Ovulation Stage;O),以及已排卵卻呈退化現象之退化期(Atretic Stage;A)。針對卵細胞在不同發育階段的雌魚個體測定其血清中以及整個卵細胞和細胞內各項滲透離子(Osmolytes)的含量,以資明瞭隨卵細胞的發育過程及卵內滲透壓(Osmolarity)變化時,其各項成化變化之相互關係。本實驗採用自然成熟的香魚(ayu, Plecoglossus altivelis)作為實驗材料,挑選其卵細胞在適當發育時期的健康成魚,從尾柄動脈抽取血液並以解剖方式篩選及分離卵粒。主要的測定項目為血清中離子(Electrolytes)含量、滲透壓(Osmolarity)和其所含之遊離胺基酸(Free amino acids)等濃度,以及卵細胞之水分含量、卵徑大小、卵內有機物及灰分含量、卵細胞離子濃度及卵內遊離胺基酸等含量。測定的結果為隨卵細胞之發育過程,卵內水分含量有逐漸增加的趨勢,可由YGI時之58.0%達到排卵期的69.7%,而退化期時其水分含量更高達到79.4%。卵徑大小與卵內水分含量呈正相關,卵內滲透壓則與卵內水分含量呈負相關,二者與卵水分含量均在GVM期時有一顯著的變化。在不同卵細胞發育階段時,卵內有機物及灰分含量與卵乾重的比值無顯著性的變化,血清中之滲透壓亦無顯著的變化。卵細胞離子含量除鎂(Mg)外其餘均有顯著的變化,其中又以鈉離子(Na)含量最高,有隨發育之過程而增加的現象。血清中離子除鈉外亦有顯著性的變化,但無明顯的變化趨勢。卵內遊離胺基酸除Alanine及Glutamic adic外其餘均有顯著性地增加現象而血清內者除Serine, Proline及Lysine外均無顯著的變化。血清中之總遊離胺基酸含量無明顯的變化趨勢,而卵內者則有顯著增加的現象。非必須遊離胺基酸的比值在血清中有隨卵細胞發育過程而增加的現象,但在卵內者則下降。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:34Z (GMT). No. of bitstreams: 0 Previous issue date: 1988 | en |
dc.description.tableofcontents | 目錄………………………………………………………………I 照片目錄………………………………………II 表目錄………………………………………………………………………………………III 圖目錄…………………………………………………………………………………………V 摘要…………………………………………………………………………………………VII 第一章 緒言……………………………………………………………………………… 第二章 材料與方法……………………………………………………………………… 第三章 結果……………………………………………………………………………… 第四章 討論……………………………………………………………………………… 謝辭………………………………………………………………………………………… 參考文獻…………………………………………………………………………………… | |
dc.language.iso | zh-TW | |
dc.title | 香魚,P1ecog1ossus a1tivelis,卵細胞發育成熟過程中滲透壓調節之研究 | zh_TW |
dc.date.schoolyear | 76-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 71 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
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