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  1. NTU Theses and Dissertations Repository
  2. 生命科學院
  3. 生化科學研究所
請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75662
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dc.contributor.authorYun-Jin Jiangen
dc.contributor.author江運金zh_TW
dc.date.accessioned2021-07-01T08:14:30Z-
dc.date.available2021-07-01T08:14:30Z-
dc.date.issued1988
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dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75662-
dc.description.abstract從鯉魚水晶蛋白的分析知道含有高甲硫胺酸(high-methionine)的γ水晶蛋白。由其cDNA庫篩選兩個高甲硫胺酸菌株(high-methionine clone):γ-ml,γ-m2。兩者的cDNA與翻譯的蛋白質序列相類性(homology)皆為75%。
牛蛙蝌蚪在變態時水晶蛋白有顯著的變化。一是α與γ水晶蛋白佔總蛋白質量降低,二是-β水晶蛋白(BC3或β29)由少於百分之一總蛋白質量增至百分之五點五左右。分析此一蛋白質,發現它與牛的β Bp有相當高的相類性(homology)。用相類性比較定序(Sequencing by homology)定出了約75%的蛋白質序列,這75%中與β Bp的相類性為80%
文中並對此二種蛋白質的結構、演化角色與可能功能作一討論。
zh_TW
dc.description.abstractCarp lens contain unusually high methionine (12-15%) γ-crystallins. We got two γ-crystallins clones of such high-methionine contents from the cDNA library of carp, that is, γ-m1 and γ-m2. The amino acid and nucieotide sequence homology between γ-m1 and γ-m2 are about 75%.
The crystallin patterns of bullfrog undergo visible changes during its metamorphosis. One change is the decrease of contents of α-and γ-crysta1lins; the other is the increase of a β-crystallin (BC3 or β29), that is, from less 1% of total protein to 5.5% of total protein. analysing and sequencing by homology, we determined 75% sequence of this protein. In this 75% sequence the protein has 80% homology with βBp of bovine.
The stucture, evolution and possible function of these proteins are discussed.
en
dc.description.provenanceMade available in DSpace on 2021-07-01T08:14:30Z (GMT). No. of bitstreams: 0
Previous issue date: 1988
en
dc.description.tableofcontents一、中文摘要……………………………………1
二、緒言……………………………………2
三、材料藥品與儀器………………………4
四、實驗方法……………………………………7
五、結果……………………………………13
六、討論……………………………………18
七、圖表及說明……………………………………23
八、英文摘要……………………………………49
九、誌謝……………………………………50
十、參考文獻……………………………………51
dc.language.isozh-TW
dc.title鯉魚及牛蛙水晶蛋白之研究zh_TW
dc.titleStudies on Crystallins of Carp and Bullfrongen
dc.date.schoolyear76-2
dc.description.degree碩士
dc.relation.page57
dc.rights.note未授權
dc.contributor.author-dept生命科學院zh_TW
dc.contributor.author-dept生化科學研究所zh_TW
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