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Effects of Spermine on the Conformation of Plasmid pBR 322
|Publication Year :||1986|
本實驗中，以spermine處理supercoil pBR322,經CD spectroplarimeter分析，得到與控制組幾乎相反的圖譜，顯示出spermine可引起pBR 322的結構改變，但我們仍然無法確知pBR322的結構到底發生了何種變化，只能推測它可能是一種Z-DNA like的結構。
Interest has been increasing during the last twenty years in the naturally occurring polyamines, putrescine (1，4-diaminohutane), spermidine, and spermine. Their specific function is still obscure, but their ubiquitous distribution, their high concentration in cells, and the increase in the concentrations found in rapidly growing tissues have stimulated many investigations on these compounds.
The most pressing questions in the polyamine field are: What is the physiological role of the polyamines, and what is the mechanism of their action The most obvious specific characteristic of the polyamins is their polybasic character, which gives them a much higher affinity for acidic constitutents than that exhibited by Na+, K+, Mg2+, Ca2+, or monoamines; this polybasic character is most pronounced with spermine because of its four positive groups. It seems very likely that the polybasic nature of the polyamines is very important in determining their physiological action. In this experiment, the spermine treated supercoil pBR322 produced a near-inversion of the UV circular dichroism spectrum, indicated that the spermine can induce conformation change of pBR322, but we still do not know what conformation it changed into, we just infer that it may be a z-DNA like conformation.
|Appears in Collections:||生化科學研究所|
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