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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Yu-Nu Shih | en |
| dc.contributor.author | 史育女 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:14:02Z | - |
| dc.date.available | 2021-07-01T08:14:02Z | - |
| dc.date.issued | 1986 | |
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(1983) Protein phosphatases: properties and role in cellular regulation. Science, 221, 331-338. 17. Kimura, T. (1981) ACTH stimulation on cholesterol side chain cleavage activity of adrenocortical mitochondria Mol. Cellul. Biochem. 36, 105-122. 18. Kishimoto, A. Takai, Mori, T., Kikkawa, U., and Nishizuka, Y. Activation of calcium and phospholipid-dependent protein kinase by diacylglycerol, its possible relation to phosphatidyl-inositol turnover. J. Biol. Chem. 255, 2273-2276. 19. Mahaffee, D., Reitz, R. C. and Ney, R. L. (1974) The mechanism of action of adrenocorticotropic hormone J. Biol. Chem. 249, 233. 20. Neher, R. and Milani, A. (1978) Mol. and Cell. Endocrinol. 9, 243-253. 21. Paul, D. P., Gallant, S., Orme-Johnson, N. R., Orme. Johnson, W. H., and Brownie, A. C. (1976) Temperature dependence of cholesterol binding to cytochrome P-450 of the rat adrenal. J. Biol. Chem. 251, 7120-7126 (1976) 22. Pymond, M. and Hofmann. F. 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(1983) Inhibitor protein of the cAMP-dependent protein kinase: characteristics and purification Methods in Enzymology. 99, 81-93. 34. Whitehouse, S. Walsh, D. A. (1982) Punfication of a physiological form of the inhibitor protein of the cAMP-dependent protein kinase J. Biol. Chem. 257, 6028-6032. 35. 柯逢春（1983) 鯉魚頭腎皮質固酮之研究 台灣大學生化科學研究所 碩士論文 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75586 | - |
| dc.description.abstract | 本實驗目的在探討鯉魚頭腎蛋白激?A (cAMP-dependent Protein Kinase，簡稱pkA)的特性，於實驗過程中，也發現頭腎組織含有可抑制pkA活性的抑制物，即蛋白激?A抑制蛋白(cAMP-dependent protein kinase inhibitor ，簡稱pkI)，因此本實驗也對pkI進行初步探討。 頭腎於4mM EDTA-15mMβ-mercaptoethanol (pH7.0)以均質機勻碎，離心棄沈澱得粗分均質液，再以DE-52滌流之。發現pkI活性見於120-200 mM及360-450 mM NaCl滌流區。本實驗曾兩次層析頭腎組織的pkA活性，發現兩次結果頗有差異：第一次組織所得之pkA活性全不受cAMP的影響，而第二次的層析，則見有仰賴cAMP的pkA，此項結果顯示鯉魚頭腎組識中含有兩種型式的pkA。 鯉魚頭胃組織的pkI也可用DE-52管柱層析之，pkI活性見於150?300mM CH3COONa滌流區，所得pkI不具去磷酸?之活性。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:14:02Z (GMT). No. of bitstreams: 0 Previous issue date: 1986 | en |
| dc.description.tableofcontents | 摘 要………………………………………………………………………………………1 緒 言………………………………………………………………………………………2 材料與方法…………………………………………………………………………………5 一、實驗動物……………………………………………………………………………5 二、化學藥品……………………………………………………………………………5 三、重要儀器……………………………………………………………………………6 四、實驗方法……………………………………………………………………………7 Ⅰ、粗分均質液……………………………………………………………………7 Ⅱ、醋酸沈澱………………………………………………………………………7 Ⅲ、離子交換層析…………………………………………………………………7 A、製備pkA……………………………………………………………………7 B、製備pkI……………………………………………………………………8 Ⅳ、測定pkA或pkI的活性…………………………………………………………8 結 果………………………………………………………………………………………10 一、分離pkA與建立pkA活性測定系統…………………………………………………10 二、pkI 分離……………………………………………………………………………11 三、排除pkI抑制活性來自去磷酸?的可能性 ………………………………………12 討論…………………………………………………………………………………………13 參考文獻……………………………………………………………………………………18 | |
| dc.language.iso | zh-TW | |
| dc.title | 鯉魚頭腎蛋白激?A及其抑制物活性的初步研究 | zh_TW |
| dc.title | Preliminary Study on the Protein Kinase A and its Inhibitor of Carp Head Kidney | en |
| dc.date.schoolyear | 74-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 42 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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