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標題: | 藍綠藻的固氮作用之生理研究 Physiological Study on Nitrogen Fixation of Blue-Green Algae |
作者: | Lin-Lan Chen 陳鈴蘭 |
出版年 : | 1986 |
學位: | 碩士 |
摘要: | Anabaena HA101, HA405和HA501在液態通氣培養下,只有HA101可以生長穩定且可形成均勻懸液,?較佳的實驗材料。HA101在mBG0-11培養液中,有較佳的生長速率,而在SM0培養液中,則有較好的固氮活性。在SM0培養液中pH值鹼性HA101及HA405有較佳的生長,而以中性情況下,HA101及HA405的固氮活性較好。在培養液不含任何氮源情況下,藍綠藻固氮作用與其異胞數目有密切關係,但於異胞老化時,二者之間的相關性則減少。含氮培養液中,NO3-或NH4+均可抑制異胞之形成與固氮?之固氮活性,而NH4+對藍綠藻之影響比NO3-對藍綠藻之影響大。藍綠藻固氮作用與光合作用密切相關,而與氫氣產生量成負相關的現象。固氮作用所需能量主要來源是呼吸作用。用KCN及CO抑制藍綠藻固氮作用的程度比用DCMU藥品抑制效果來得大。此呼吸作用抑制固氮活性較大的原因,可能是固氮作用能源ATP的需求及氧氣的累積(氧氣可抑制固氮?的活性)。 Anabaena HA101 is the best one of three strains (HA101, HA405 and HA501) for this study because this strain grew well and could be made homogeneous suspension easily without breaking the filaments. HA101 had a better growth rate in liquid mBG0-11 medium, but had a better nitrogen fixing activity in SM0 medium. Both strains HA101 and HA405 grew better in alkaline than in acidic or neutral culture medium. However, the optimum PH for their nitrogen fixing activities was the neutral one. In the absence of N- source in medium, the nitrogen fixation of Anabaena was parallely related with number of heterocysts in filaments. Combined nitrogen, NO3- or NH4+, inhibited the formation of heterocysts and activity of the nitrogenase. NH4+ had more severe inhibitory effects than NO3- on Anabaena. Rates of nitrogen fixation of Anabaena followed the rates of photosynthesis but the rates of nitrogen fixation were negatively related with the rate of hydrogen production. The energy needed for nitrogen fixation derives from the respiratory activity. The rates of nitrogen fixation were more severly inhibited by respiratory inhibitors, such as KCN and CO, than by the photosynthetic inhibitor, such as DCMU. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75574 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
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