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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75559完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 古鎮鈞 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:13:52Z | - |
| dc.date.available | 2021-07-01T08:13:52Z | - |
| dc.date.issued | 1986 | |
| dc.identifier.citation | 1. Ahmed, H and S. F. Graham (1977). Persistent infections in L cells with temperature-sensitive mutants of reovirus. J. Virol. 23, 250-262. 2. Ahne, W. (1978). Isolation and characterization of infectious pancreatic necrosis virus from pike (Esox lucius). Archives of Virology. 58, 65-69. 3. Bellett, A. J. D., P. D. Cooper (1959). Some properties of the transmissible interfering component of vesicular stomatitis virus preparation. J. Gen. Microbiol. 21, 498-509. 4. Chen, S. N., S. C. Chi, Y. Ueno and G. H. Kou (1983. 6). A cell line derived from tilapia ovary. Fish Pathology 18(1)13-18. 5. Dobos, P. (1976). Size and structure of the genome of IPNV Nucleicacids Res. 3, 1903-1924. 6. Dorson, M., J. Castric and C. Torchy (1978). Infecious pancreatic necrosis virus of salmonids: biological and antigenic feature of of a pathogenic strain and of a non-pathogenic variant selected in RTG-2 cells. J. Fish Diseases, 1, 309-320. 7. Gercel, C., K. B. Mahan, and V. V. Klamp arian. (1985). Preliminary characterization of a persistent infection of Hela cells with human rhinovirus type 2. J. gen. Virol. 66, 131-139. 8. Gould, E. A., A. C. Chanas, A. Buckley and G. R. Varma. (1983). Immunofluorescencece studies on the antigenic interreleationships of the hughes virus serogroup (genus nairovirus) and identification of a new strain. J. gen. Virol. 64, 739-742. 9. Hackett, A. J. (1964). A possible morphological basis for the autointerference phenomenon in vesicular stomatitis virus. Virology 24, 51-59. 10. Hackett, A. J. F. L. Schaffer, and S. H. Madin. (1967). The seperation of infectious and autointerfering particles in vesicular stomatitis virus preparation. Virology 31, 114-119. 11. Hedrick, R. P., J. L. Leong and J. L. Fryer (1978). Persistent infection in salmonid fish cells with infectious pancreatic necrosis virus (IPNV). Journal of Fish Disease 1, 297-308. 12. Hedrick, R. P. and J. L. Fryer (1981). Persistent infection of three salmonid cell lines with infectious pancreatic necrosis virus (IPNV). Fish Path. 15, 163-172. 13. Hedrick, R. P. and J. L. Fryer (1982). Persistent infection of salmonid cell lines with infectious pancreatic necrosis virus (IPNV): a model for the carrier state in trout. Fish Path. 16, 163-172. 14. Hedrick, R. P.., J. L. Fryer, S. N. Chen and G. H. Kou (1983). Characteristics of four birnaviruses isolated from fish in Taiwan. Fish Path. 18(2)91-97. 15. Hedrick, R. P., W. D. Eaton, J. L. Fryer, Y. C. Hah, J. W. Park and S. W. Hong. (1984). Biochemical and serological properties of birnavirus from fish in Korea. (private communication). 16. Henle, W., and G. Henle. (1943). Interference of inactive virus with the propagation of virus of influenza, Science 98, 87-88. 17. Henle, G., Deinhardt, F., and Henle, W. (1958). Study on persistent infections of tissue culture. I. General aspects of the system, J. Exp. Med. 108, 537-560. 18. Holland, J. J. and L. P. Villarreal (1975). Purfication of defective interfering T particles of vesicular stomatitis and rabies viruses generated in vivo in brains of newborn mice. Virology 67:438-449. 19. Holland, J. J., Kennedy, S. I. T., Semler, B. L., Jones, C. L., Roux, L. and Grabau, E. A. (1980). Defective interfering RNA virus and host-cell response. In Comprehensive Virology, vol. 16, pp. 137-192. 20. Huang, A. S. and R. R. Wagner (1966). Defective T particles of vesicular stomatitis virus, 2, biological role in homologous inerference. Virology 30, 173-181. 21. Huang, A. S. and E. K. Manders (1970). Defective viral particles and viral disease processes. Nature, (London) 226, 325-327. 22. Kennedy, J. C. and R. D. Macdonald. (1982). Persistent infection with infectious pancreatic necrosis virus mediated by defective-interfering (DI) virus paarticles in a cell line showing strong interference but little DI replication. J. gen. Virol. 58:361-371. 23. Kimbrall, P. C. (1982). In Virology. PP. 362-366. Yi Hsien Publishing Co. Taiwan, R. O. C. 24. Lazzarini, R. A., J. D. Keene and M. Schubert (1981). The origins of defective interfering particles of the negetive-strand RNA virus. Cell 26, 145-154. 25. Lo, C. F. and Y. W. Hong. (1984). The characterization of the virus isolated from gill of clam, Meretrix I. usoria. Master thesis. Fu-Jen Catholic University, Taiwan, R. O. C. 26. Lindgerding, B. C., S. R. Pheeps, and W. B. Schill. (1984). Fish cell lines: Characterization by isozyme analysis. in vitro. 20(3):167-171. 27. Lightner, D., and P. George. (1969). Morphological characteristics of infectious pancreatic necrosis virus in trout pancreatic tissue. J. Fish. Res. Bd. Canada 26:2247-2250 28. Liu, K. C. 1981. Piscine erythrocytic nnecrosis virus in culitured eel Anguilla japonica in Taiwan. Proc. Natl. Sci. Counc. (B). R. O. C. 5(4), 365-368. 29. Marcus, P. I. and Sekellick, M. J. (1977). Defective interfering particles with covalently linked (+-) RNA induce interferon. Nature (London) 226, 815-619. 30. Macdonald, R. D. (1978). Ring plaque formation in infectious pancreatic necrosis virus correlates with defective interfering particle production. J. gen. Virol. 41, 623-628. 31. Macdonald, R. D. and T. Yamamoto. (1978). Quantitative analysis of defective interfering particles in infectious pancreatic necrosis virus preparations. Archives of Virology 57:77-89. 32. Macnonald, R. D. and J. C. Kennedy. (1979). Infectious pancreatic necrosis virus persistently infects chinook salmon embryo cells independent of interferon. Virology 95:260-264. 33. Macdonald, R. D. (1980). Immunofluorescent detection of double-stranded RNA in cells infected with reovirus, infectious pancreatic necrosis virus, and infectious bursal disease virus. Can. J. Microbiol. 26, 256-261. 34. Macnonald, R. D. and D. A. Gower. (1981). Genomic and phenotypic divergence among three serotypes of infectious pancreatic necrosis virus. Virology 114:187-195. 35. Merrill, C. R., D. Goldman, S. A. Sedman and M. H. Ebert. (1981). Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211:143-144. 36. Moss III, L. H., and M. Gravell. (1969). Ultrastructure and sequential development of infectious pancreatic necrosis virus. J. Virol.. 3:52-58. 37. Nicholson, B. L. and D. James. (1974). Homologous viral interference in trout and altantic salmon cell cultures infected with IPN virus. J. Virol. 180-182. 38. Nicholson, B. L. and R. Dexter. (1975). Possible interference in the isoltion of IPN virus from carrier fish. J. Fish. Res. Board Can. 32:1437-1439. 39. Nonyama, M., Y. Watanabe, and A. F. Graham. (1970). Defective virions of reovirus. J. Virol. 226-236. 40. Nonyama, M., and A. F. Graham. (1970). Appearance of defective virons in clones of reovirus. J. Virol. 693-694. 41. Okamoto, N., T. Sano, R. P. Hederick and J. L. Fryer. (1983). Antigentic releationshipa of selected strains of IPNV and eel virus European (EVE). Jounal of Fish Disease 6:19-25. 42. Reborts, T. E., and P. Dobos. (1983). Study on the mechanism of temperature sensitivity of infectious pancreat necrosis virus replication. J. gen. Virol. 64:331-339. 43. Reed, L. J. and H. Muench. (1938) A simple method of estimating fifty percent endpoints. Amer. J. Hyg. 27:493-497. 44. Reno, P. W., S. Darley, and M. Savan. (1978). infectious pancreatic necrosis :experimental induction of a carrier staste in trout. J. Fish Res. Board CAN 35:1451-1456. 45. Rima, B. K. and S. J. Martin. (1976). Persistent infection of tissue cultured cell by RNA viruses. Med. Microbiol. and Immum. 162, 89-118. 46. Rovozzo, G. C., and C. N. Burke. (1973). In A Manual of Basic Virological Techniques. PP. 82-93. Prentice-Hall. Inc. Englewood Cliffs, N. J. 47. Sekellick, M. J. and p. I. Marcus. (1978). Persistent infection interferon-inducing defective-interfering particles as mediators of cell sparing: possible role in persistent infection by vesicular stomatitis virus. Virology 85, 175-186. 48. Shigeharu, K., D. Kurosawa, I. Kunimine, K. Nobusawa, and S. Kobayashi. (1973). Electron microscopic observations of. the pancreas and liver in the fingerling rainbow trout with symptoms of IPN. Japanese Journal of Ichthyology. 20(3). 163-177. 49. Siciliano, M. J., and C. R. Shaw. (1976). Seperation and visualization of enzyanes on gels. In Chromatographic and electrophoretic techniques 4th edition. (I. smith, ed.) William Heinemann Medical Books Ltd., London. 2, 185-209. 50. Stark, C. and S. I. T. Kennedy. (1978). The generation and propagation of deffective interfering particles of semliki forest virus in different cell types. Virology 89, 285-299. 51. Stampeer, M., D. Baltimore, and A. S. Huang. (1971). Absence of interference during high-multiplicity infection by clonally purified vesicular stomatitis virus. Journal of Virology. 7, 407-411. 52. Tu, Kuen-Ching, R. S. pendlove, and R. W. Goede. (1974). Immunofluorescent cell assay of infectious pancreatic necrosis virus. Microbiology. 27(4), 593-599. 53. Stewart II, W. E. (1981). Interferon assay. In The Interferon system. the 2nd edition. Springer- verleg/wien. 54. Ueno, Y., S. N. Chen, G. H. Kou, R. P. Hedrick and J. L. Fryer. (1984). Characterization of a virus isolated from Japanese eel Anguilla japonica with nephroblastoma. Bull. Inst. Zool., Academia Sinica, 23, 47-55. 55. Wolf, K. and M. C. Quimby (1962). Established eurythermic line of fish cells in virto. Science, 135, 1065-1066 56. Wolf, K., and M. C. Quimy (1966) Lymphocystis virus; Isolation and propagation in centrarchid fish cell lines. Science. 151, 1004-1005. 57. Wu, J. L., and S. Y. Wu. (1983) Electrophoretic differences of esterase isozyme from the surface mucus of oreochromis fish Bull. Inot. Zool., Academia sinica. 22(2):133-140. 58. von Magnus, P. (1954): Incopmplete forms of influenza virus Adv. Virus Res. 2, 59-79. 59. Yamamoto, T. and J. listoff. (1979): Infectious pancreatic necrosis virus: quantification of carrier in lake populations during a 6-year period. J. Fish. Res. Board Can. 36:562-567. 60. Zhadnov, V. M. (1975): Integration of viral genome. Nature (London), 256, 471-473. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75559 | - |
| dc.description.abstract | 本研究的目的擬建立感染性胰臟壞死病毒持久性感染吳郭魚卵巢細胞株(IPNV Persistent infectious TO-2 cell line)並研究其生長特性。 利用自鰻腺分離出來的感染性胰臟壞死病毒(FV-1229)感染吳郭魚卵巢細胞株(TO-2)，可以?立病毒持久性感染細胞株，命名為P1/TO-2。其特徵為可持久性的釋放病毒顆粒（P1病毒）於培養基中，對同源病毒具有抗性，但可接受異源病毒的攻擊性感染，由電顯觀察及螢光免疫實驗發現P1/TO-2細胞株中只有少數細胞含有病毒抗原。 由外部形態及對胞內LDH, MDH, G6PDH和Esterase四種同功?的電泳分析，均無法區分P1/TO-2及TO-2細胞株。電子顯微鏡觀察雖發現P1/TO-2細胞株中只有少數細胞的細胞質含有病毒顆粒，但在細胞碎片中則常可發現病毒群，此結果顯示P1/TO-2細胞株培養基內的病毒是由少數含病毒顆粒的細胞破裂而來。 P1病毒和原來的FV-1229病毒均可為抗FV-1229兔血清完全中和，顯示二者在血清型上並無差別，其他如電顯觀察，溫度適應性質實驗及病毒寄主範圍實驗，亦無法判斷二者的不同。然二者在增殖過程中均有自我幹擾現象發生，實驗推論病毒族群中含有缺陷性幹擾顆粒(Defective interfering particle)，此種顆粒可能為P1/TO-2細胞株建立及維持生長的主要因數。用含有抗FV-1299兔血清的培養基培養P1/TO-2細胞株，無法治療甚至降低細胞株中含病毒的細胞數目。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:52Z (GMT). No. of bitstreams: 0 Previous issue date: 1986 | en |
| dc.description.tableofcontents | 摘要…………I 謝辭…………III 目錄……………IV 內容…………1?3 第一章緒言1 第二章 材料與方法 第一節 材料 2.1-1 細胞株……………6 2.1-2 病毒……………6 2.1-3 培養基……………7 2.1-4 磷酸鹽緩衝液………8 2.1-5 胰蛋白?溶液………8 2.1-6 TSA培養基………………9 2.1-7 TNE緩衝液……………9 2.1-8 電子顯微鏡標本製備試劑………10 2.1-9 受?質混合液………………12 第二節 方法 2.2-1 細胞的繼代培養……………15 2.2-2 病毒力價的測定………………16 2.2-3 持久性感染細胞株的建立………16 2.2-4 TO-2及P1/TO-2細胞之掃描式電子顯微鏡觀察……17 2.2-5 穿透式電子顯微鏡…………18 1．細胞感染病毒的觀察…………18 2．細胞的觀察………………19 2.2-6 螢光抗體實驗…………19 2.2-7 P1/TO-2對各種病毒的接受性………20 2.2-8 同功?的電泳分析…………………20 2.2-9 細胞的冷凍貯藏及解凍後約釋放病毒力價的測定……22 2.2-10 P1病毒對四種細胞的感受性………23 2.2-11 溫度實驗…………23 2.2-12 自我幹擾作用…………23 2.2-13 病毒的純化……………24 2.2-14 抗FV-1229病毒血清的製作……………25 2.2-15 P1/TO-2細胞株與抗體的作用…………26 第三章 結果 3.1 持久性感染細胞株的建立…………………27 3.2 形態及生長特性…………………28 1．光學顯微鏡及電子掃描式顯微鏡的觀察 2．穿透式電子顯微鏡的觀察 3.3 螢光抗體實驗………………………29 3.4 P1/TO-2對各種病毒的接受性……29 3.5 同功?的電泳分析…………………29 3.6 細胞冷凍貯藏及解凍後病毒力價的測定……30 3.7 釋放感染性病毒…………………30 3.8 P1病毒對四種細胞的感受性…………31 3.9 溫度實驗……………………31 3.10 自我幹擾作用………………32 3.11 P1/TO-2細胞株與抗體的作用………33 第四章 討論………………34 圖表……………38 參考文獻…………56 | |
| dc.language.iso | zh-TW | |
| dc.title | 感染性胰臟壞死病毒對TO-2細胞的持久性感染研究 | zh_TW |
| dc.title | Persistent Infection in TO-2 Cells with IPN Virus Isolated from Japanese Eel (Anguilla Japonica) | en |
| dc.date.schoolyear | 74-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 60 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| 顯示於系所單位： | 動物學研究所 | |
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