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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | 劉新 | zh_TW |
dc.date.accessioned | 2021-07-01T08:13:31Z | - |
dc.date.available | 2021-07-01T08:13:31Z | - |
dc.date.issued | 1984 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75498 | - |
dc.description.abstract | 本研究之目的乃在於對中華肝吸蟲囊狀幼蟲做一個廣泛的研究,由其外部形態,囊鞘構造,出囊機制,從而建立一個體外培養的系統,俾能提供對中華肝吸蟲生理,生化,發育等方面的瞭解。另外並研究成蟲體內磷酸脂酵素之分佈情形。 剛由克氏鱎魚肉內取出的中華肝吸蟲囊狀幼蟲之囊胞為橢圓形透明的構造(138.2μm×114.7μm),以掃描電子顯微鏡觀察,囊胞表面平滑,囊鞘由三層囊壁所組成;外壁又可細分為兩層。用組織化學方法分析囊鞘壁的組成係由蛋白質及黏多醣等物質所組成,但無脂質的成份。 影響中華肝吸蟲囊狀幼蟲出囊作用的絕對因數為溫度(37℃)及胰蛋白?。 中華肝吸蟲囊狀幼蟲之體外培養係以CHO細胞株及大白鼠肺臟初級細胞培養內的存活期較長,可達50天。若以合成培養基及添加物的培養,則其存活期約30天,且其存活率亦較低。 以掃描電子顯微鏡觀察,囊狀幼蟲體表被有鱗狀的構造,在身體橫向成規則排列,愈往末端愈細而成刺狀。體表有兩種感覺構造:因為頂端有一根感覺毛的感覺乳突。(A)為頂端無感覺毛的圓形蕈狀乳突。(A)及(B)之分佈以體前端尤其是以口吸盤周圍為多。成蟲體表無鱗狀或刺狀的構造,則滿佈結節狀圓狀物的分佈。體前端有橫向不規則的稜紋。體表有感覺乳突的分佈也密生於口吸盤附近。 成蟲體內磷酸酯?的分佈情形:酸性磷酸酯?普遍存在於身體各部份,以消化道活性最強,其次體表,皮下組織,生殖系統及吸盤等處均有顯著的活性。鹼性磷酸酯?則僅於卵巢及排泄管有活性。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:31Z (GMT). No. of bitstreams: 0 Previous issue date: 1984 | en |
dc.description.tableofcontents | 摘要 謝辭 目錄 內容 第一章 緒言…………………….1 第二章 材料與方法………………….4 2.1 中華肝吸蟲囊狀幼蟲及成蟲之收集………………4. 2.2 中華肝吸蟲囊狀幼蟲囊胞構造及出囊機制………5 2.2.1 中華肝吸蟲囊狀幼蟲囊胞構造…………5 2.2.1.1 光學顯微鏡觀察……………………5 2.2.1.2 掃描電子顯微鏡觀察……….5 2.2.1.3 穿透電子顯微鏡觀察………6 2.2.1.4 囊鞘壁組織化學觀察……….7 2.2.1.4.1 蛋白質的檢定……………7 2.2.1.4.2 黏多醣的檢定……….7 2.2.1.4.3 脂質的檢定…………8 2.2.2 囊狀幼蟲之出囊機制……………8 2.2.2.1 溫度的影響………………8 2.2.2.2 酸鹼度的影響……………9 2.2.2.3 胃蛋白?的影響……………9 2.2.2.4 胰蛋白?的影響…………9 2.2.2.5 膽鹽的影響………………10 2.3 囊狀幼蟲之體外培養………………10 2.3.1 囊狀幼蟲之準備及消毒…………10 2.3.2 大白鼠肺細胞之初級培養………11 2.3.3 CHO細胞株的培養……………12 2.3.4 囊狀幼蟲之體外培養技術…………12 2.3.5 囊狀幼蟲生長發育的評估………….13 .4 囊狀幼蟲與成蟲體表之掃描顯微鏡之觀察………13 2.5 中華肝吸蟲成蟲體內磷酸酯?活性的測定……15 2.5.1 鹼性磷酸鹽酵素活性的測定………15 2.5.1 1 Azo-Coupling Method……….15 2.5.2 酸性磷酸酯?活性的測定………16 2.5.2. 1 Azo-Coupling Method………16 2.5.2.2 Gomori Method……………16 第三章 中華肝吸蟲囊狀幼蟲囊胞構造及體外出囊機制……18 3.1 中華肝吸蟲囊狀幼蟲囊胞構造………18 3.2 囊狀幼蟲體外出囊機制………………19 3.3 討論………………21 第四章 中華肝吸蟲囊狀幼蟲之體外培養……………25 4.1 培養基及添加物對囊狀幼蟲體外生長的影響…25 4.2 囊狀幼蟲在不同細胞培養中的生長……….26 4.3 討論……………26 第五章 中華肝吸蟲囊狀幼蟲及成蟲體表之超顯微掃描觀察….29 5.1 幼蟲體表之觀察………29 5.2 成蟲體表之觀察……30 5.3 討論………………….31 第六章 中華肝吸蟲成蟲體內磷酸酯?活性之研究………33 | |
dc.language.iso | zh-TW | |
dc.title | 中華肝吸蟲囊狀幼蟲及成蟲之超顯微構造,出囊機制,體外培養及組織化學之研究 | zh_TW |
dc.title | Studies on the Outer Ultrastrueture, Excystation Mechanism, In Vitro Cultivation and Histochemistry of Metacercaria and Adult of Clonorchis sinensis | en |
dc.date.schoolyear | 72-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 69 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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