請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75409
標題: | 台灣水域日本鯷系群之分子及形態分析 Molecular and morphological analysis on Japanese anchovy stocks around Taiwan |
作者: | Shang-Zhan Lee 李尚展 |
出版年 : | 2003 |
學位: | 碩士 |
摘要: | 棲息於台灣沿岸的日本鯷是其東海族群之端末分支,其仔魚因漁業的利用而受到相當強大的漁獲壓力。本論文藉由瞭解日本鯷經不同洄游路徑抵達台灣,在東、西部海域形成不同系群之關係,加以探討宜蘭灣日本鯷之春季群及秋季群是否為同一母族群所源出。 本分析至少取得133個有效樣本,分別是春季宜蘭灣(1E)44個,春季海峽(1W)44個,及秋季宜蘭灣(3E)45個。分析結果顯示,740個胞色素b表示的單套型岐異度均大於0.995;分子歧異度為0.0141(1E), 0.0130(1W) 及0.0144(3E),顯示各群鹼基位之歧異度並不高。全體個體間之鹼基數差異平均為10.5±4.8(SD)位,呈單峰正規分佈。群間的變異佔全部變異的4.1%(Fst=0.041),但呈現顯著差異,暗示群間具有可區別的分化:1E群與3E群之距離最大,其次為1E群與1W群,但1W群及3E群則顯著性測驗不達5%。以微隨體對位基因分析發現,其對位基因數多達152-302個雙鹼基,除了1E群外,各群內均偏離哈溫平衡,為異形合子不足。形態分析經主成分變方匯集後,第一主成分軸分得81.6%之變異,而第二及第三主成分則共占13.1%之變異;逕以第二軸及第三軸之數值表現,三群之表型(phenotype)並不可區分。本實驗結果推論,宜蘭灣之春、秋系群不是由同一個母族群所從出,亦即其春、秋群間具有分化之痕跡。由FST及RST所反應之海峽系群與秋季宜蘭灣群的綜合性指數顯示,其間之差異不大,海峽與宜蘭灣之日本鯷可用一個均一的系群(stock)加以處理。秋季宜蘭灣群與春季海峽群間之洄游率為10.8-25.0%之間。 The coastal populations of Japanese anchovy are marginal distribution of the fish in the East China Sea. Larval Japanese anchovy suffers huge fishing pressure since 1976 when artisan fishing shifted to commercial pair-trawlers. In order to obtaining fundamental information for reasonable management, I analyze the stock differences between Strait and Ilan Bay, and cohort differences between spring and autumn in the Ilan Bay. At least 133 effective samples without missing data was categorized into 3 groups of spring Ilan Bay (1E), spring Strait (1W) and autumn Ilan Bay (3E). Categorized analysis on cytochrome b gene indicated that haplotypic gene diversity indices greater than 0.995, but molecular diversity indices are low; 0.0141 (1E), 0.0130 (1W) and 0.0144(3). Pair-wise base difference is estimated to 10.5±4.8, showing a normal-type distribution. Although between-group variation takes 4.1% of the total, however group differentiation is supported by 5% significant tests. Microsatellite genes are highly diversified as measured by various allelic numbers of 152-302 di-nucleotides, most of the loci deviated from H-W equilibrium as heterozygote deficiency. Principal analysis on the morphometric variables indicated a subtle phenotypic difference. Based on the finding, I postulate that the spring and autumn anchovy larvae are cohorts originated from 2 spawning populations; the fishes from the Strait and autumn Ilan Bay could be treated as a single unit, and there migration rates are estimated 10.8-25.0%. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75409 |
全文授權: | 未授權 |
顯示於系所單位: | 動物學研究所 |
文件中的檔案:
沒有與此文件相關的檔案。
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。