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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.author | Yoichiro Ueno | en |
| dc.contributor.author | 上野洋一郎 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:13:03Z | - |
| dc.date.available | 2021-07-01T08:13:03Z | - |
| dc.date.issued | 1982 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75406 | - |
| dc.description.abstract | 本研究乃嘗試利用EK-1細胞及RTG-2細胞自罹患腎腫瘤之日本鰻（Angu11a japonica）分離病毒。並進行病毒特性之試驗及鑑定。 實驗結果顯示由腎腫瘤病鰻組織包括肝、腎、脾臟及生殖腺所分離出之病毒對50-75℃之溫度具有抵抗性，不會為有機溶劑（乙醚、氯仿）降低共活性。其對酸性、中性則其活性很穩定，但對較高的PH值，如10.0、11.0 ，稍微有感受性。用電子顯微鏡觀察，則可發現病毒之形狀是六角形，其大小直徑約為78nm。用抗EVE家免血清做中和試驗及螢光抗體試驗的結果，證明本病毒之血清型與EVE相符。由以上結果證實本病毒為EVE。 將實驗病毒注射於鯉魚、吳郭魚及鰻魚時，發現本病毒在低水溫時對吳郭魚及鰻魚、鯉魚具有致病力，且由實驗組的死亡魚體中可再分離出病毒，但卻不能發現肉眼可見的病徵。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:13:03Z (GMT). No. of bitstreams: 0 Previous issue date: 1982 | en |
| dc.description.tableofcontents | 一、摘要…………………i 二、謝辭…………………ii 三、目錄…………………iii 四、內容 第一第 緒論…………………1 第二章 材料與方法…………………6 2.1 培養基…………………6 2.2 細胞之繼代培養…………………7 2.3 50％組織細胞病毒感染（TCID50／ml)之計算…………………9 2.4 初級病毒分離…………………10 2.5 病毒之繼代…………………11 2.6 電子顯微鏡觀察…………………12 2.7 酸鹼度之影響…………………13 2.8 有機溶劑之影響…………………14 2.9 病毒對高溫之穩定性…………………15 2.10 對紫外光之感受性…………………15 2.11 中和試驗…………………16 2.12 螢光抗體法…………………17 2.13 冷凍、解凍試驗…………………18 2.14 病原性試驗…………………19 第二章 結果…………………20 3.1初次病毒分離…………………20 3.2 電子顯微鏡觀察…………………20 3.3酸鹼度之影響…………………21 3.4有機溶劑之影響…………………21 3.5對高溫之穩定性…………………21 3.6對紫外光之感受性…………………22 3.7中和試驗…………………22 3.8螢光抗體法…………………22 3.9冷凍、解凍試驗…………………22 3.10病原性試驗…………………23 第四章 討論…………………43 五、參考文獻…………………49 | |
| dc.language.iso | zh-TW | |
| dc.title | 日本鰻腎腫瘤所分離出病毒之研究 | zh_TW |
| dc.title | Studies of virus isolated from Japanese eel (Anguilla iaponica)with nephroblastoma | en |
| dc.date.schoolyear | 70-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 54 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 動物學研究所 | zh_TW |
| Appears in Collections: | 動物學研究所 | |
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