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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | TE-CHUNG LIU | en |
dc.contributor.author | 劉德中 | zh_TW |
dc.date.accessioned | 2021-07-01T08:12:36Z | - |
dc.date.available | 2021-07-01T08:12:36Z | - |
dc.date.issued | 1981 | |
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Katz, Y, and Eckstein, B, (1974) Changes in the steroid concentration in blood of female Tilapia aurca (Teleostei, Cichlidae) during initiation of spawning. Endocrinology 95 : 963—967. 22, Lupo di Prisco, C. and Chieffi, G. (1965) Identification of’ steroid hoiones in the gonadal eztract of the synchronous hermaphrodite teleost, Serranus scriba Gen. Comp, Endocrinol. 5 : 698_699, 23. Lindner, H. R. and Mann, T, (1960) Relationship between the content of androgenic steroids in the testes and the secretory activity of the seminal vesicles in the bull, J. Endocrinol, 21 : 341—360, 24. Lindner, H. R. (1963) Partition of androgen between the lymph and venous blood of the testis in the ram, J. Endocrinol 25 : 483—494. 25.. Muller, C. H. (1977) Plasma 5-dihydrotestosterone and testosterone in the bullfrog, Raua Catesbeiana : Stimulation by bullfrog LH, Gen.Comp. Endocrinol, 33: 122-132. 26, Maung, L. W. and Follet, B. K. (1977) Effects of chicken and ovine luteinizing hormone on androgen release and cyclic AMP production by isolated cell from the quail testis. Gen, Comp, Endocrinol, 33 : 242—253. 27. Moustafa, A, M. and Koritz, S. B. (1977) Steroids on the stimulation by Ca++ and the inhibition by ADP of steroid 11β-hydroxylation in adrenal mitochondria. Eur. J. Biochem. 78 : 231—238. 28. Resko, J. A., Feder, H. A. and Goy, R. W. (1968) ndro— gen concentrations in plasma and testes of developing rats, J, Endocrinol, 40 : 485—491, 29. Savard, K., Dorfman, R. I., Baggett, B., Fielding,L. L., Engel, L. L., Mcpherson, H. T., Lister, L. H., Johnson,D. S., Hanblen, E. C. and Engel, F. L. (1960) Clinical. morphological and biochemical studies of a viriizing tumor in the testis. J, Clint Invest, 39 : 534—553, 30 Sangalang, G, B,, Freeman, H, C. andFlemming, R. B. (1978) A simple technique for determining the sex of fish by radioimmumoassay using 11—ketotestosterone antiserum. Gen. Comp, Endocrinol. 36 : 187—193. 31. Schmidt, R. J. and Idler, D. R. (1962) Steroid hormones in the plasma of salmon at various stages of maturation comp, Endocrinol. 2 : 204—214. 32, Setchell, B. P., Waites, G. M. H. and Linduer, H. R. (1965) Effect of undernutrition on testicular blood flow and metabolism and the output of testosterone in the ram, J. Reprod. Fertil. 9 : 149—162. 33. Tamaoki, B. I., Inauo, H and Nakaro, H. (1969) In vitro synthesis and conversion of androgens in testicular tissue, in “gonads” Ed. by McKerns, K. .W. pp 547—613, 34, Van den Hurk, R. and Lambert, J. G. D. (1977) Biosynthesis of 11β—hydroxy and 11-ketoandrogens in the testis of the black molly. Poecilia (=MOllienisia) latipinna, Comp. Biochem, Physiol.56B: 117—118. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75306 | - |
dc.description.abstract | 本文研究的目的是在探討雄鯉魚雄性素的組成。希望能清楚的知道雄鯉魚在生殖季節透過GTH(簡字見於文後附表)的作用引發何種雄性素的生合成,來執行生殖功能。 首先字雄鯉魚睾丸萃取類固醇經高壓液相色層分析儀作定性定量分析,分析了六種雄性素總含量為 0.83μg/100g,其中KT占70%。其他五種含量很低。GTH處理後雄鯉睾丸內雄性素激增至7.96μg/100g。約為對照組的十倍。 自雄鯉魚血漿萃取類固醇,發現血漿內雄性素只有KT 含量為17.84μg/100ml。GTH處理後,血漿內雄性素總含量增為23.06μg/100ml。增量為1.3倍。和睾丸組織的增量(十倍)比較起來,相差很遠。而GTH處理後睾丸與血漿之雄性素比例也不一致。此現象引發出一個問題:假如睾丸的雄性素能等比例的釋放到血液循環系統中,則不該在比例上與不一致的現象發生,發生的可能原因為雄性素彼此間在循環系統中的代謝清楚速率快慢不一。所以GTH處理後在血漿測得的雄性素在比例和增量上和睾丸內差距頗大。基於以上之假設。設計以下之實驗:將睾丸組織作培養,測量睾丸組織與培養液之間雄性素釋放的情況以及GTH處理後增量比例是否一致來判別活體睾丸及血漿內經GTH處理後雄性素增量和比例上的差異造成的結果。 至目前止僅分析了培養液中雄性素的組成。但是在以T為素材的轉化反應實驗中,發現培養組織內雄性素含量約?培養液中的四倍。而比例上則大致相同。以上事實說明睾丸和血漿內雄性素的增量和比例上的差異是由釋放率和代謝清除率不等所造成的結果。 表一的資料同時顯示GTH處理後能提高睾丸內T,A,OHT,OHA及KA的含量。使人認?之五種物質可能是合成KT的前驅物質或者是中間產物。於是進而觀察這五種物質被睾丸組織轉化成KT的能力。結果發現它們或多或少都能被轉化成KT。以材料消失快慢來比慢,其轉化率為A>T>KA>OHT>OHA 而以KT形成量之多寡來比較則為KA>T>OHT>A>OHA 以上實驗同時發現了它們彼此間互相轉變的能力.又引發出新的問題:T和AD何者是生合成KT的起始物質.它們是依循那些主要的途徑生合成KT.於是設計以下實驗:以不同量(5-160μg)的T和睾丸組織一起培養四小時.觀察各雄性素的?量和T之間的關係.發現T經過OHT到達KT是生合成KT的主要途徑.而且T轉變為OHT的速率大於OHT轉變為KT的速率.並且將T,AD,OHT,OHA和睾丸組織培養更長的時間 (8小時),結果發現A轉化成KT,OHA轉化成KT的效率比T轉化成KT,OHT轉化成KT差. 根據以上二實驗之資料,可以說在雄性鯉睾丸生合成KT的反應是以T為主要的前驅物質以OHT為中間?物最後生合成KT. 附表: T:睾固酮 OHT:氫氧基睾固酮 KT:酮基睾固酮 A:睾二酮 OHA:氫氧基睾二酮 KA:酮基睾二酮 GTH:性腺刺激素 | zh_TW |
dc.description.abstract | The main purposes of this study are aimed to investigate the effect of GTH on carp androgen production from the aspects of the changes in total amount and composition and to investigate the biosynthesis pathway of KT by incubating various androgens as substrates with carp testis in vitro. Androgen analysis is performed by HPLC throughout this study. KT is the predominant androgen (70%) in the control testis .The total androgen content is 0.81μg/100 g. The in vivo GTH treatment can greatly affect the content and composition of testicular androgens. The total content increases about 10 folds. Although KT is still the major androgens (40%), Yet OHT (27.14%) and OHA (15.82%) become very prominent. In comparison with testicular androgens, the androgens of carp plasma are very simple. KT is the only androgen found, but in vivo GTH treatment can bring an additional appearance of small amount of T and A. The content of plasma androgen before and after vivo GTH treatment is 17.84 and 23.06μg/l00 ml, respectively, The in vitro GTH treatment can also greatly affect the content and composition of androgens released from testis into culture medium. Carp testis can convert T, A, OHT, OHA and KA to other forms of androgens and KT is the final product. The conversion rate of the above substrates is in the following sequence: A > T > KA >OHT >OHA. On the other hand, the substrate efficiency is in the sequence of KA > T >OHT >A = OHA. Therefore, oxygenation rather than hydroxylation at C 11 is the rate limiting step for KT biosynthesis. It is postulated that KT is synthesized through T via OHT. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:36Z (GMT). No. of bitstreams: 0 Previous issue date: 1981 | en |
dc.description.tableofcontents | 1 Abstracts . . . . . . . . . . . . . . . . . . . . . . . . 1 2 Introductions . . . . . . . . . . . . . . . . . . . . . 3 3. Materials and Methods . . . . . . . . . . . . . . 6 4. Results. . . . . . . . . . . . . . . . . . . . . . . . 13 5. Discussion . . . . . . . . . . . . . . . . . . . . 65 6. Summary in Chinese . . . . . . . . . . . . . 73 7. Acknowledgements. . . . . . . . . . . . . . . 75 8. Bibliography. . . . . . . . . . . . . . . . . . . . . 76 | |
dc.language.iso | zh-TW | |
dc.title | 鯉魚睪丸雄性素生成之研究 | zh_TW |
dc.title | STUDIES ON ANDROGEN PRODUCTION IN CARP TESTIS | en |
dc.date.schoolyear | 69-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 86 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科學研究所 | zh_TW |
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