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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Lin Wan-Zin | en |
| dc.contributor.author | 林婉真 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:12:34Z | - |
| dc.date.available | 2021-07-01T08:12:34Z | - |
| dc.date.issued | 1981 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75295 | - |
| dc.description.abstract | 台灣雨傘節(Bungarus multicinctus)蛇毒之β-神經毒蛋白（β-Bungarotoxin）經由CM-Sephadex C-25，Sephadex G-50提取精製完成。 利用CD技術來探討各種媒體對β-雨傘節神經毒的影響。發現自然分子的CD光譜表現典型的α結構，在低溶性溶劑如1,2-ethane-diol中，其立體結構與水溶液相似，但在6.0 M guanidine hydrochloride中，其規則的立體結構(ordered structure)變成不規則的結構(unordered structure)。 β-雨傘節蛇毒由兩個蛋白質分子鍵（A和B）以雙硫鍵連接而成，分子量22,000左右。其A分子和磷酯酸水解?的初級結構相似，比較各種磷酯酸水解?與β-雨傘節蛇毒α結構的穩定性，再利用CNBr修飾法由A蛋白質鍵打掉8個胺基酸，並測定其α結構的穩定性。比較修飾過後的蛇毒與自然蛇毒的毒性，可以得知A和B蛋白質分子鍵間有交互作用。 | zh_TW |
| dc.description.abstract | The snake venom of Bungarus multicntus was chromatographied by CM-Sephadex C-25 into 13 fraction. Fraction X was then isolated and purified by G-50 column into 2 fractions. Peak 1 was identified to be β-Bungarotoxin. This protein containst two polypeptide chains (A chain molecular weight 13,500, B chain molecular weight 7,500). Using the CD technique to study the median effect on β-Bungarotoxin, we found that the native spectra has double minima-at 222 nm and 209 nm which is Characteristic of α-helical structure. Determination of helix-coil transition of PLA, β-Bungarotoxin and CNBY-β-Bungarotoxin indicated that there is interaction between A- and B- polypeptide Chain. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:34Z (GMT). No. of bitstreams: 0 Previous issue date: 1981 | en |
| dc.description.tableofcontents | LIST OF FIGURES……………………………………………………………………………ii I.INTRODUCTION……………………………………………………………………………1 II.MATERIALS AND METHODS…………………………………………………………………4 1. Venom and Reagents ………………………………………………………………4 2. Important Instruments……………………………………………………………4 3. Isolation and Purification β-Bungarotoxin ………………………………6 4. Disc Electrophoresis ……………………………………………………………6 5. Amino Acid Analysis………………………………………………………………6 6. Circular Dichroism Studies ……………………………………………………7 7. Cleavage with Cyanogen Bromide ………………………………………………8 8. Toxicity Test………………………………………………………………………8 III. RESULTS 1. Isolation and Purification of β-Bungarotoxin……………………………9 2. CD of β-Bungarotoxin and its derivatives…………………………………16 3. Fission of β-Bungarotoxin with Cyanogen Bromide ………………………18 4. CD of β-Bungarotoxin after Cyanogen Bromide cleavage…………………19 5. Helix-Coil Transition……………………………………………………………19 6. Biological characterization……………………………………………………23 7. Molecular Shape of β-Bungarotoxin …………………………………………24 IV. DISCUSSION 1. Conformational Stability ………………………………………………………28 2. Subunit interaction………………………………………………………………30 V. SUMMARY …………………………………………………………………………………34 1. English………………………………………………………………………………34 2. Chinese………………………………………………………………………………35 VI. ACKNOWLEDGMENTS ……………………………………………………………………36 VII. REFERENCE ………………………………………………………………………………37 | |
| dc.language.iso | zh-TW | |
| dc.title | 雨傘節神經毒的生化研究 | zh_TW |
| dc.title | Biochemical Studies Of β-Bungarotoxin | en |
| dc.date.schoolyear | 69-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 45 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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