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DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chung Yang -Ts'ung | en |
dc.contributor.author | ?楊聰 | zh_TW |
dc.date.accessioned | 2021-07-01T08:12:28Z | - |
dc.date.available | 2021-07-01T08:12:28Z | - |
dc.date.issued | 1981 | |
dc.identifier.citation | 1. Lee, C. Y. (1972) Ann. Rev. Pharmacol. 12,265-286 2. Tu, A Y. (1977) ? Venoms: Chemistry and Molecular Biology ? pp. 257-320, John Wiley and Sons, New York. 3. Narita, K., and Lee, C. Y. (1970) Biochem. Biophys.Res. Commun. 41,339-344 4. Kaneda, N., Sasaki, T., and Hayashi, K. (1976) Biochem.Biophys. Res. Commun.72,1450-1455 5. Yang, C. C.(1974) Toxicon 12,1-43 6. Tu, A. T.(1973) Ann. Rev. Biochem. 42,235-258 7. Chen, Y. H., Lu, Y. C., and Lo, T. B.(1975) J. Chinese Biochem. Soc. 4,69-82 8. Hung, M. C., and Chen, Y. H. (1977) Int. J. Peptide Protein Res.10,277-285 9. Hung, M. C. , Pan, Y. H. , Cheng, K. L. , and Chen, Y. H.(1978) Biochim. Biophys. Acta 214,355-363 10. Condrea, E. (1974) Experienta 30,121-129 11. Chen, Y. H., Hsu, C., and Lin, C. K. (1980) European J. Cell Biol.22,222 12. Chen, Y. H., Hsu, C., and Lin, C.K. (1980) J. Chinese Biochem. Soc.9,17p-18p 13. Chen, Y. H., Hsu, C., Lin, C. K., Lai, M. Z., and Kao,L. S. (1980) unpublished experiments. 14. Lo, T. B., and Chang, W. C. (1975) Toxicon 13,108 15. Lo, T. B., Chen, Y. H., and Lee, C. Y. (1966) J. Chinese Chem. Soc. 13,25-37 16. Hung, M. C., and Chen, Y. H. (1977) Int. J. Peptide Protein Res. 10,277-285 17. Davis, B. J. (1964) Ann. N. Y. Acad. Sci.121,404-427 18. Beutler, E., and Baluda, M. C. (1964) Blood 23, 688-697 19. Karl, D. M., and Holm-Hansen, D. (1976) Anal. Biochem.75,100-112 20. Deluca, M., and McElray, W. D. (1974) Biochemistry 13,921-925 21. Palek, J., Liu, S. C., and Synder, L. M. (1978) Blood 51,385-395 22. Dodge, J. T., Mitchell, C., and Hanahan, D. J. (1963) Arch. Biochem. Biophys.100,119-130 23. Steck, T. L.(1972) J. Mol. Biol.66,295-305 24. Peacock, A. C., and Dingman, C. M. (1968) Biochemistry 7,668-674 25. Kurantsin-Mills, J., and Lessin, L. S. (1981) Biochim. Biophys. Acta 641,129-137 26. Fairbanks, K., Steck, T. L., and Wallach, D. F. H. (1971) Biochemistry 10,2606-2607 27. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall R. J. (1951) J. Biol. Chem.193,265-275 28. Hanahan, D. J. (1973) Biochim. Biophys. Acta 300,319-340 29. Grazitt, Y., Ohad, I., and Loyter, A.(1975)Biochim. Biophys. Acta 382,65-73 30. Condrea, E., De Vries, A., and Mager, J. (1964) Biochim. Biophys. Acta 84,60-73 31. Condrea, E. (1980) “ Snake Venoms ” pp.448-479 Springer- Verlag Berlin Heidelberg, New York. 32. Condrea, E. (1974) Experienta 30,121-129 33. Elgsaeter, A., Shotton, D. M., and Branton, D. (1976) Biochim. Biophys. Acta 426,101-122 34. Gull, S., and Smith, A. D. (1972) Biochim. Biophys. Acta 288,237-246 35. Klibansky, C., London, Y., Frenkel, A., and De Vries, A. (1968) Biochim. Biophys. Acta 150,15-23 36. Carlsson, F. H. H. and Louw, A. I. Biochim. Biophys. Acta 534,322-330 37. Hung, M. C., Cheng, K. L., Lai, M. Z., and Chen, Y. H. (1977) J. Chinese Biochem. Soc.6,27-36 38. Wong, C. H., Ho, C. H., and Wang, K. T. (1980) J Chinese Biochem. Soc.9,25-29 39. Frish, A., Gazitt, Y., and Loyter, A.(1973) Biochim. Biophys. Acta 291,690-700 40. Martin, J. K., Luthra, M. G., Wells, M. A., Watts, R. P., and Hanahan, D. J. (1975)Biochemistry 14,5400-5408 41. Patel, T. N., Braganca, B. M., and Bellare, R. A. (1969) Experimental Cell Research 57, 289-297. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75273 | - |
dc.description.abstract | The action of Taiwan cobra cardiotoxin on intact human erythrocytes in plasma expander devoid of any inorganic salts had been investigated by Hsu & Lin in our laboratory. As indicated by them, a lag period existed between the time of addition of CTX and the time of onset of hemolysis and the hemolysis was preceded by the depletion of endogeneous ATP in erythrocytes. ( European J. Cell Biol.(1980) 22,222) In fact, because short of energy source material plasma expander can't afford a power to maintain the ATP levels of erythrocytes, the phenomenum of ATP depletion during the process of interaction of CTX with red cells that eventually results in hemolysis was worth reexamining. Using a buffer in which erythrocyte APP levels remain constant by at least 5 h to undertake the experiment, I found there is no signs for the depletion of intracellular ATP preceding the hemolysis induced by the cardiotoxin. A similar conclusion was achieved by performing the experiment in a buffer devoid of energy sources in a different way. An additional investigation into energy levels of the erythrocyte showed that the hemolysis induced by CTX occurred more rapidly under conditions where the intracellular ATP levels were higher. This specificity of CTX action on erythrocytes was manifested when compared with that of synthetic basic polypeptide, poly-L-lysine. It was found further that the depletion of endogenerous ATP accompanied the formation of aggregates containing spectrin. This implies that the hemolytical activity of CTX may have something to do with the status of some membrane proteins situated in cytoplasmic surface. To check whether membrane structure of erythrocytes incubated with CTX has been changed before hemolysis, the synergistic action of CTX with PLA was also studied. The result obtained shows that the rate of hemolysis was enhanced at its maximum when the molar ratio of CTX and PLA is 1:1. Based on above observations, together with the results of studying the interaction of CTX with liposomes by. others, I suggest that CTX can penetrate through cell membranes and target some proteins bound to the cytoplasmic surface of membranes. In addition, the effect regarding ionic composition of various buffer media on the extent of hemo- lysis was studied in this report. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:28Z (GMT). No. of bitstreams: 0 Previous issue date: 1981 | en |
dc.description.tableofcontents | LIST OF ABBREVIATIONS………………………iii LEGENDS OF FIGURES AND TABLES…………iv SUMMARY ( in English )………A-1 SUMMARY ( in Chinese )………A-3 INTRODUCTION………………………………………1 MATERIALS AND METHODS………………………6 1. Materials………………………………………………6 2. Isolation and Purification of Cardiotoxin…………7 3. Disc Gel Electrophoresis…………………………8 4. Preparation of Buffers………………………………9 5. Collection of Erythrocytes……………………………9 6. Determination of Cellular ATP Content………………11 7. Hemolysis Measurement………………………………12 8. Preparation of Erythrocyte Ghosts………………………14 9. Electrophoretic Analysis of Membrane Proteins…………14 10. Protein Determinations………………………17 11. Experimental Design………………………17 RESULTS AND DISCUSSION……………………19 1. Purification and Homogeneity of Cardiotoxin 19 2. Search For the Buffer In Which Erythrocyte ATP Levels Can Keep Constant…………19 3. Investigations Into the Phenomenum of ATP Depletion………………………21 4. Hemolysis Studies……………………………………………………………25 5. Synergistic Action of CTX and PLA……………………………………………30 6. Membrane Protein Associations During Metabolic ATP Depletion…………35 7.Ionic Effects on Hemolysis and Erythrocyte ATP Levels……………………38 REFERENCES ACKNOWLEDGMENTS APPENDICES | |
dc.language.iso | zh-TW | |
dc.title | 在重新探討︰飯匙倩心臟毒所引發的紅血球能量耗解…問題之過程中的一些發現 | zh_TW |
dc.title | SOME FINDINGS DURING THE PROCESS OF REEXAMINING THE PROBLEM REGARDING THE ATP DEPLETION OF ERYTHROCYTES INDUCED BY COBRA CARDIOTOXIN | en |
dc.date.schoolyear | 69-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 78 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科學研究所 | zh_TW |
顯示於系所單位: | 生化科學研究所 |
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