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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75271| Title: | 豬微精漿蛋白(β-microseminoprotein)之生化研究 Biochemical Study of Porcine β-microseminoprotein |
| Authors: | Ying-Che Cheng 張瑩徹 |
| Publication Year : | 2002 |
| Degree: | 碩士 |
| Abstract: | 利用逆相層析法，由豬的精漿中分離出兩種具有抑制精子活動能力的蛋白質（Jeng等，1993）。此兩種蛋白質以質譜儀分析，其質量分別為10067.0與10068.0，相差1個dalton，而利用蛋白質等電點分析，發現其等電點分別為7及7.5，由胺基酸序列分析發現此兩種蛋白互為異構物（趙，1997），而且為已知的蛋白質－微精漿蛋白(β-microseminoprotein; β-MSP)。本實驗運用基因選殖的技術將β-MSP cDNA載入表現質體中，並產製出重組β-MSP蛋白(recombinant β-MSP Two sperm motility inhibitors have been purified from porcine seminal plasma by reversed-phase HPLC. (Jeng et al., 1993) The molecular mass of the two proteins determined by electrospray mass spectrometry are 10067.0 and 10068.0. These proteins are differ |
| URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75271 |
| Fulltext Rights: | 未授權 |
| Appears in Collections: | 生化科學研究所 |
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