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標題: | 生長激素調控?郭魚組織內類胰島素生長因數第一型及其結合蛋白質第三型之表現 Tilapia(Oreochromis mossambicus) Insulin-like growth factor-I and Insulin-like growth factor binding protein-3 tissue expression pattern after recombinant growth hormone treatment |
作者: | Kai-Ming Chang 張凱明 |
出版年 : | 2000 |
學位: | 碩士 |
摘要: | 類胰島素生長系統(insulin-like growth factor system)對於脊椎動物的胚胎發育過程及成長扮演著非常重要的角色。整個類胰島素生長系統主要由下列成分所組成。(l)類胰島素生長因數(insulin-like growth factors, IGFs), (2)類胰島素結合蛋白(insulin-like growth factor binding proteins, IGFBPs), (3)類胰島素蛋白?(IGFBP IGFBPPs),(4)類胰島素受體(insulin-like growth factor receptors)。而其中類胰鳥素生長因數(IGFs)主要受生長激素(growth hormone)的調控,因此生長激素-類胰島素系統軸基因(growth hormone-IGF system axis gene)之間有著非常密切的關係。類胰島素生長因數的生物活性主要是經由細胞膜表面的類胰島素受體結合後,所產生的下游訊息傳遞所達成的。在已知哺乳類中,肝臟是最主要的類胰島素生長因數的生產者及調節者。在血液中99%以上的類胰烏素生長因數(IGFs)是和類胰島素結合蛋白(IGFBPs)形成複合體(complex),而其中的類胰島素第三型結合蛋白(IGFBP-3)就佔了90%以上。此一複合體的形成延長了IGFs在血液中的半衰期(half-life)。 目前,有關於魚類類胰島素生長因數的生理調控所知並不多。經由本論文之初步發現,吳郭魚中的類胰島素第一型生長因數(IGF-I)及類胰島素第三型結合蛋白(IGFBP-3)受到石斑魚生長激素(EaGH)的刺激後,其mRNA的表現均有顯著的增加這也間接證明瞭基因重組生產的石斑魚生長激素具有生物活性表現.注射24小時後,IGF-I及IGFBP-3的mRNA表現量增加是未注射前的2倍。IGF-I/IGFBP-3間的比值在生長激素3小時注射後,呈一定(其值約在0.9-1.0之間)此一數值相當於哺乳類血液中90%以上的IGFs是由IGFBP-3所形成的複合體。這是第一次在魚類中證實,肝臟經生長激素刺激後其IGF-I/IGFBP-3的mRNA表現量呈一定值。其他吳郭魚各組織器官在受生長激素刺激後,IGF-I及IGFBP-3 mRNA表現量,在腸道與胃也好會受到生長激素的影響。 In vertebrates, the insulin-like growth factor system (IGF system) plays-an important role in early embryo development and postnatal growth. The IGF system consists of the following parts. (1) ligands: insulin-like growth factor (IGF-I and II). (2) binding protein: insulin-like growth factor binding protein (IGFBPs, IGFBP-1 to -6). (3) protease: insulin-like growth factor binding protein protease (IGFBP protease, IGFBPP). (4) receptors: insulin-like growth factor receptors (IGF-I and II receptors). Among the IGF system, the IGFs are known to be regulated by growth hormone and mediate the biological function, through receptor downstream signal transduction. Therefore, the growth hormone-IGF system axis genes play important functions in any cellular level. The biological function of IGFs are mediated through membrane receptors and its downstream signal transduction. In the mammalian, the liver is the major endocrine organ to produce and regulate IGFs. In the blood circulation, more then 99% of IGFs form a complex with IGFBPs and acid-labile subunit (ALS). Among the IGFs-IGFBP-ALS complex, more than 90% of IGFBPs is the IGFBP-3, which is the dominant regulator of circulation IGFs. The IGF complex in blood is believed to extend the half-life of IGFs and help to transport the IGFs across the extracellular matrix, to reach their target tissue. To date, we still know little of fish insulin-like growth factor regulation in gene level or protein level. The objective of this thesis is to characterize the mRNA expression of IGF-I and IGFBP-3 after growth hormone treatment in Tilapia (Oreochromis mossambicus). In our preliminary results, we found that both IGF-I and IGFBP-3 is stimulated by grouper recombinant growth hormone (EaGH) at 0.5μg/g of fish body weight. In the major endocrine tissue, liver, the IGF-I and IGFBP-3 is significant increased and after 24hr injection, the amount of IGF-I, IGFBP-3 expression is doubled. The relative ratio after normalization to β-actin the IGF-I/IGFBP-3 is between 0.9-1 during the time course study period. The value of IGF-I/IGFBP-3 is approximately equivalent of that mammalian, which indicating more than 90% of IGFs is bound to IGFBP-3 in blood circulation. This is the first demonstration in fish and maybe in mammalians, that in vertebrate system. The endocrine organ liver, the mRNA ratio of IGF-I/IGFBP-3 is constant after growth hormone treatment in a time-course study. The other parts of tilapia tissue and its IGF-I/IGFBP-3 mRNA expression will also discuss in this thesis. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75264 |
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顯示於系所單位: | 漁業科學研究所 |
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