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DC 欄位 | 值 | 語言 |
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dc.contributor.author | Yu-Hsin Kao | en |
dc.contributor.author | 高玉馨 | zh_TW |
dc.date.accessioned | 2021-07-01T08:12:18Z | - |
dc.date.available | 2021-07-01T08:12:18Z | - |
dc.date.issued | 2001 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75231 | - |
dc.description.abstract | 5S rDNA為聚集型的重複性序列,每一個重複單位包含轉區及基因間空白區,轉錄區為120 bp,具高度保留性,而基因間空白區之序列在物種間的差異則很大,可用於親緣關係較近物種間的分析。本實驗以聚合酵素連鎖反應擴增各種蝴蝶蘭屬植物之基因間空白區序列,做親緣關係樹的分析,依樹型顯示蝴蝶蘭屬植物為單源發生的演化,且可分成7群,其中P. amboinensis、P. venosa和P. violacea屬於同一群,而P. equestris與Phalaenopsis節的P. amabilis、P. aphrodite、P. sanderiana、P. stuartiana、P. schilleriana則屬於另一群,此結果雖然與形態分類不符,卻與蝴蝶蘭雜交種染色體配對情形之研究、RAPD分析及核型觀察的結果一致,至於P. mannii與P. amboinensis及P. lueddemanniana三者間的親緣關係則尚待釐清。 | zh_TW |
dc.description.abstract | 5S rDNA are organized into clusters of tandem repeats with each repeat consisting of a transcribed region of about 120 bp conserved sequences and an intergenic spacer (IGS) region varying in size among species. The divergence of IGS sequences makes it useful for phylogenetic analyses. In this study, IGSs of 28 Phalaenopsis species were amplified by the polymerase chain reaction, cloned, and sequenced. Phylogenetic analysis of IGS sequences revealed that the origin of these species seemed to be monophyletic and could be divided into seven groups. Among them P. amboinensis, P. venosa and P. violacea were grouped together which was consistent with cytogenetic data and RAPD analysis. Similarly, P. equestris, P. amabilis, P. aphrodite, P. sanderiana, P. stuartiana and P. schillerana, were also considered to be closely related species. The relationships among P. mannii, P. amboinensis and P. lueddemannian remains unsolved. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2001 | en |
dc.description.tableofcontents | 中文摘要…………………………………………………………………………………………………………………i 英文摘要…………………………………………………………………………………………………………………ii 簡寫字對照表……………………………………………………………………………………………………………iii 壹、前言…………………………………………………………………………………………………………………1 貳、材料與方法 一、植物材料………………………………………………………………………………………………………8 二、DNA 的抽取……………………………………………………………………………………………………8 三、5S rDNA基因間空白區序列的擴增 …………………………………………………………………………9 四、探針的標定……………………………………………………………………………………………………10 五、選殖體的篩選…………………………………………………………………………………………………11 六、DNA 序列之定序及分析………………………………………………………………………………………15 七、親緣關係分析…………………………………………………………………………………………………15 參、結果 一、5S rDNA的IGS序列之選殖及定序……………………………………………………………………………17 二、蝴蝶蘭屬植物5S rDNA之IGS序列的比較……………………………………………………………………18 三、親緣關係之分析………………………………………………………………………………………………19 肆、討論 一、蝴蝶蘭5S rDNA之IGS序列的結構與組成……………………………………………………………………23 二、蝴蝶蘭植物種間的親緣分析…………………………………………………………………………………24 三、5S rDNA的演化 ………………………………………………………………………………………………27 伍、參考文獻……………………………………………………………………………………………………………28 圖次 圖1、依蝴蝶蘭P.aphrodite的5S rDNA基因序列設計之引子位置及序列……………………………………34 圖2、蝴蝶蘭屬植物5S rDNA之IGS序列選殖體的篩選…………………………………………………………35 圖3、P.celebensis三個選殖體序列之對齊排列………………………………………………………………36 圖4、P.cornu-cervi四個選殖體序列之對齊排列 ……………………………………………………………37 圖5、P.equestris五個選殖體序列之對齊排列 ………………………………………………………………38 圖6、P.amboinensis五個選殖體序列之對齊排列 ……………………………………………………………39 圖7、P.fasciata五個選殖體序列之對齊排列…………………………………………………………………40 圖8、P.gigantea五個選殖體序列之對齊排……………………………………………………………………41 圖9、P.lowii三個選殖體序列之對齊排列 ……………………………………………………………………42 圖10、P.viridis三個選殖體序列之對齊排列…………………………………………………………………43 圖11、P.venosa五個選殖體序列之對齊排列 …………………………………………………………………44 圖12、P.schilleriana三個選殖體序列之對齊排列 …………………………………………………………45 圖13、P.stuartiana五個選殖體序列之對齊排列 ……………………………………………………………46 圖14、P.micholitzii五個選殖體序列之對齊排列……………………………………………………………47 圖15、P.pallens四個選殖體序列之對齊排列…………………………………………………………………48 圖16、P.maculata五個選殖體序列之對齊排列 ………………………………………………………………49 圖17、P.mariae五個選殖體序列之對齊排列 …………………………………………………………………50 圖18、P.lueddemanniana六個選殖體序列之對齊排列 ………………………………………………………51 圖19、P.lued-pulchra五個選殖體序列之對齊排列 …………………………………………………………52 圖20、P.violacea五個選殖體序列之對齊排列 ………………………………………………………………53 圖21、P.modesta五個選殖體序列之對齊排列…………………………………………………………………54 圖22、P.fuscata三個選殖體序列之對齊排列…………………………………………………………………55 圖23、P.mannii五個選殖體序列之對齊排列 …………………………………………………………………56 圖24、P. sanderiana五個選殖體序列之對齊排列……………………………………………………………57 圖25、P. parishii五個選殖體序列之對齊排列………………………………………………………………58 圖26、Doritis pulcherrima五個選殖體序列之對齊排列……………………………………………………59 圖27、P.aphrodite九個選殖體序列之對齊排列………………………………………………………………60 圖28、P.sumatrana五個選殖體序列之對齊排列………………………………………………………………61 圖29、P.stobartiana四個選殖體序列之對齊排列……………………………………………………………62 圖30、P.amabilis四個選殖體序列之對齊排列 ………………………………………………………………63 圖31、P. lobbii四個選殖體序列之對齊排列…………………………………………………………………64 圖32、二十九個分類單元的一致性序列之對齊排列 …………………………………………………………65 圖33、蝴蝶蘭屬植物5S rDNA之IGS-NJ樹………………………………………………………………………70 圖34、蝴蝶蘭屬植物5S rDNA之358個最佳MP樹之一 …………………………………………………………71 圖35、蝴蝶蘭屬植物5S rDNA之IGS-MP樹………………………………………………………………………72 圖36、三十二個分類單元的一致性序列之親緣關係樹 ………………………………………………………73 圖37、十五種植物5S rDNA之IGS序列 …………………………………………………………………………74 表次 表1、本實驗所使用的28種蝴蝶蘭與一種朵麗蘭屬植物………………………………………………………76 表2、本實驗所使用的28種蝴蝶蘭及一種朵麗蘭屬植物之代號及篩選之選殖體數目………………………78 表3、5S rDNA之IGS序列在各種植物種內及種間的相似度……………………………………………………79 附錄 附錄1、各個分類單元選殖體間的相似度………………………………………………………………………81 附錄2、蝴蝶蘭屬植物5S rDNA之IGS序列的矩陣距離…………………………………………………………87 | |
dc.language.iso | zh-TW | |
dc.title | 以5S rDNA基因間空白區序列探討蝴蝶蘭屬植物之親緣關係 | zh_TW |
dc.title | Phylogeny of Phalaenopsis species based on 5S rDNA intergenic spacer sequences | en |
dc.date.schoolyear | 89-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 105 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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