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標題: | UNC-73的in vitro功能及ced-10V12在線蟲touch cells所扮演的角色 In vitro function of UNC-73 and the role of ced-10V12 in C. elegans touch cells |
作者: | Nei-Yin Weng 翁乃茵 |
出版年 : | 2001 |
學位: | 碩士 |
摘要: | CED-10屬於Rho family GTPases的成員。而線蟲的ced-2, ced-5, ced-10, 和ced-12基因調控了細胞屍體吞噬過程以及生殖器官頂端細胞(distal tip cells)的遷移過程。雖然活體外的實驗顯示,活化的Rac會引發Swiss 3T3纖維母細胞的細胞膜表面皺褶的形成,然而對於活體內Rac的功能尚有許多探討的空間。當異位且過量表達constitutively activated ced-10(ced-10V12)時,線蟲的六個感受機械性感覺細胞(mechanosensory touch receptor neurons)會不正常的生長;而當表達過量的ced-10基因時,則會出現不同於ced-10V12的突變表型,如在尾部出現多餘的感覺細胞。這可能是由於細胞譜系(cell lineage)遭到改變所致。 unc-73在C. elegans的細胞遷移過程(cell migration)以及指引神經走向的過程(axon guidance)扮演著重要的角色。藉由生化實驗,我們證明瞭UNC-73的第一個DH domain對CED-10/Rac1具有催化GDP置換成GTP的能力。除此之外,也證明瞭在活體外,UNC-73可以和CED-5結合,再加上我們的遺傳分析,我們推測,UNC-73可能連接上游的訊息,進一步去活化CED-10,促使C. elegans活體內細胞遷移的過程。 CED-10 belongs to Rho family GTPases. In C. elegans, ced-2, ced-5, ced-10 and ced-12 control phagocytosis of cell corpses and migration of gonadal distal tip cells. Although in vitro function of Rac has been shown to regulate a signal transduction pathway in Swiss 3T3 cells that links extracellular signals to the formation of lamellipodia, the in vivo function of Rac remains to be explored. I found that ectopic expression of constitutively activated ced-10 (designated ced-10V12) results in many defects in six touch cells in C. elegans. Interestingly, ced-10 overexpression causes different phenotype from that of ced-10V12, such as extra PLM-like cell body. This may due to the alteration of cell lineage. unc-73 is required for cell migrations and axon guidance in C. elegans. Biochemical experiment suggests that the first DH domain of UNC-73 activates CED-10. In addition, we showed that UNC-73 and CED-5 physically interact in vitro, together with our genetic data, raising the possibility that UNC-73 may link upstream signaling complexes and CED-10, mediates cell migration in C. elegans. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75207 |
全文授權: | 未授權 |
顯示於系所單位: | 動物學研究所 |
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