請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75153完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 賴正晏 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:12:01Z | - |
| dc.date.available | 2021-07-01T08:12:01Z | - |
| dc.date.issued | 2000 | |
| dc.identifier.citation | Bieberstein,U., Berbner,T., Islinger,M. and T.Braunbeck. (1999). Immunohistochemical localization of vitellogenin in rainbow trout (Oncorhynchus mykiss) hepatocytes using immunofluorescence. The Science of the Total Enviroment 233, 67-75. Castellanos-Serra,L.R., Fernandez-Patron,C., Hardy,E., and Huerta,V. (1996). A procedure for protein elution from reverse-stained polyarcylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis. Electrophoresis 17, 1564-1572. Chang,Y.S., Wang,S.C., Tsao,C.C., and Huang,F.L. (1996). Molecular cloning, structural analysis, and expression of carp ZP3 gene. Mol. Reprod. Dev. 44, 295-304. Chang,Y.S., Hsu,C.C., Wang,S.C., Tsao,C.C., and Huang,F.L.(1997). Molecular cloning, structural analysis, and expression of carp ZP2 gene. Mol. Reprod. Dev. 46, 258-267. Chang,Y.S., Weng,J.W., Li,C.C., and Huang,F.L. (1998). Identification of cystatin as a component of carp chorion. Mol. Reprod. Dev. 51,430-435. Chang,Y.S., Lu,L.F., Lai,C.Y., Kou,Y.H, and Huang,F.L. (1999). Purification, characterization, and molecular cloning of an outer layer protein of carp fertilization envelope. Mol. Reprod. Dev. 54, 186-193. Edge,A.S., Faltynek,C.R., Hof,L., Reichert,L.E., Jr., and Weber,P.(1981). Deglycosylation of glycoproteins by trifluoromethanesulfonic acid. Anal. Biochem. 118, 131-137. Fernandez-Patron,C., Castellanos-Serra,L., and Rodriguez,P. (1992). Reverse staining of sodium dodecyl sulfate polyacrylamide gels by imidazole-zinc salts: sensitive detection of unmodified proteins. Biotechniques 12, 564-573. Fernandez-Patron,C., Calero,M., Collazo,P.R., Garcia,J.R., Madrazo,J., Musacchio,A., Soriano,F,.Estrada,R., Frank,R., and Castellanos-Serra,L.R. (1995). Protein reverse staining:high-efficiency microanalysis of unmodified proteins detected on electrophoresis gels. Anal. Biochem. 224, 203-211. Franco,C., Francesca,A., Maurizio,B., and Carla,L.L. (1988) Structure and composition of the fish egg chorion (Carassiusαuratus). J. Ultrastruct.Res. 99, 79-95. Gerber-Huber,S., Nardelli,D., Haefliger,J.A., Cooper,D.N., Givel,F., Germond,J-E., Engel,J., Green,M.N., and Wahli,.W. (1987)Precursor-product relationship between vitellogenin and the yolk proteins as derived from the complete sequence of a Xenopus vitellogenin gene .Nucleic Acids Res. 15(12):4737-60. Goulas,A., Triplett,E.L., and Taborsky,G. (1996). Isolation and characterization of a vitellogenin cDNA from rainbow trout (Oncorhynchus mykiss) and the complete sequence of a phosvitin coding segment. DNA Cell Biol.15, 605-616. Grbac-Ivankovic,S., Antonijczuk,K., Varghese,A.H., Plowman,M.C., Antonijczuk,A., Korza,G., Ozols,J., and Sunderman,F.W., Jr. (1994). Lipovitellin 2 beta is the 31 kD Ni(2+)-binding protein (pNiXb) in Xenopus oocytes and embryos. Mol. Reprod. Dev. 38, 256-263. Guraya,S.S. (1982). Recent progress in the structure, origin, composition, and function of cortical granules in animal egg. Int. Rev. Cytol.78, 257-360. Hardy,E., Santana,H., Sosa,A., Hernandez,L., Fernandez-Patron,C., and Castellanos-Serra,L. (1996). Recovery of biologically active proteins detected with imidazole-sodium dodecyl sulfate-zinc (reverse stain) on sodium dodecyl sulfate gels. Anal. Biochem. 240, 150-152. Hartling,R.C. and Kunkel,J.G. (1999). Developmental fate of the yolk protein lipovitellin in embryos and larvae of winter flounder, Pleuronectes americanus. J. Exp. Zool. 284, 686-695. Heppell,S.A., Denslow ND Folmar LC Sullivan CV. Universal assay of vitellogenin as a biomarker for environmental estrogens. (1995).Environ Health Perspect 103 Suppl 7, 9-15. Het Schip,F.D., Samallo,J., Broos,J., Ophuis,J., Mojet,M., Gruber,M., and AB, G. (1987). Nucleotide sequence of a chicken vitellogenin gene and derived amino acid sequence of the encoded yolk precursor protein. J. Mol. Biol.196, 245-260. Kellner,R. and Lottspecich,F. Microcharacterization of proteins. (1994). Kudo,S. (1982).Ultrastucture and ultracytochemistry of fertilization envelope fonnation in the carp egg. Dev Growth Differ 24(4), 327-339. Luo,Z., Annikka,L.K., Itkonen,A., and Maenpaa,P.H. (1997). Active nuclear transport of chicken lipovitellin-2. Biochem. Biophys. Res. Commun. 234, 760-763. Mann,C.J., Anderson,T.A., Read,J., Chester,S.A., Harrison,G.B., Kochl,S., Ritchie,P.J., Bradbury,P., Hussain,F.S., Amey,J., Vanloo,B, Rosseneu,M., Infante,R., Hancock,J.M., Levitt,D.G., Banaszak,L.J., Scott,J., and Shoulders,C.C. (1999). The structure of vitellogenin provides a molecular model for the assembly and secretion of atherogenic lipoproteins. J. Mol. Biol. 285, 391-408. Matsubara,T., Ohkubo,N., Andoh,T., Sullivan,C.V., and Hara,A.(1999). Two fonns of vitellogenin, yielding two distinct lipovitellins, play different roles during oocyte maturation and early development of barfin flounder, Verasper moseri, a marine teleost that spawns pelagic eggs. Dev. Biol. 213, 18-32. Montorzi,M., Falchuk,K.H., and Vallee,B.L.(1995). Vitellogenin and lipovitellin: zinc proteins of Xenopus laevis oocytes. Biochemistry 34, 10851-10858. Mouchel,N., Trichet,V., Betz,A., Le Pennec,J.P., and Wolff,J.(1996). Characterization of vitellogenin from rainbow trout (Oncorhynchus mykiss). Gene 174, 59-64. Nakamura,K., Yonezawa,S., and Yoshizaki,N. (1996). Vitellogenesis-related ovary cathepsin D from Xenopus laevis: purification and properties in comparison with liver cathepsin D. Comp Biochem. Physiol B Biochem. Mol. Biol.113, 835-840. Ohlendorf,D.H., Barbarash,G.R., Trout,A., Kent,C., and Banaszak,L.J. (1977). Lipid and polypeptide components of the crystalline yolk system from Xenopus laevis. J. Biol. Chem. 252, 7922-8001. Parks,L.G., Cheek,A.O., Denslow,N.D., Heppell,S.A., McLachlan,J.A., LeBlanc,G.A., and Sullivan,C.V. (1999). Fathead minnow (Pimephales promelas) vitellogenin: purification, characterization and quantitative immunoassay for the detection of estrogenic compounds. Comp Biochem. Physiol C. Pharmacol.Toxicol. Endocrinol. 123, 113-125. Retzek,H., Steyrer,E., Sanders,E.J., Nimpf,J., and Schneider,W.J.(1992). Molecular cloning and functional characterization of chicken cathepsin D, a key enzyme for yolk formation. DNA Cell Biol.11, 661-672. Sharrock,W.J., Rosenwasser,T.A., Gould,J., Knott,J., Hussey, D., Gordon,J.I., and Banaszak,L. (1992). Sequence of lamprey vitellogenin. Implications for the lipovitellin crystal structure. J. Mol. Biol.226, 903-907. Shen,X., Steyrer,E., Retzek,H., Sanders,E.J., and Schneider,W.J.(1993). Chicken oocyte growth: receptor-mediated yolk deposition. Cell Tissue Res. 272 , 459-471. Shibata,Y., Iwamatsu,T., Oba,Y., Kobayashi,D., Tanaka,M., Nagahama,Y., Suzuki,N., and Yoshikuni,M. (2000). Identification and cDNA cloning of alveolin, an extracellular metalloproteinase, which induces chorion hardening of medaka (Oryzias latipes) eggs upon fertilization. J. Biol. Chem. 275, 8349-8354. Smeets,J.M., Rakouhi,T.R., Nichols,K.M., Komen, H., Kaminski,N.E., Giesy,J.P., and van den,B.M. (1999). In vitro vitellogenin production by carp(Cyprinus carpio) hepatocytes as a screening method for determining (anti)estrogenic activity of xenobiotics. Toxicol. Appl. Pharmacol. 157 , 68-76. Sunderman,F.W., Jr., Antonijczuk,K., Antonijczuk,A., Grbac-Ivankovic,S., Varghese,A.H., Korza,G., and Ozols,J. (1995).Xenopus lipovitellin 1 is a Zn(2+)- and Cd(2+ )-binding protein. Mol. Reprod. Dev. 42, 180-187. Tsai, Y.J., Chang, G.D., Huang, C.J., Chang, Y.S, and Huang, F.L.(1996). Purification and molecular cloning of carp ovarian cystatin. Comp Biochem. Physiol B Biochem. Mol. Biol.113, 573-580. Tyler,C.R. and Sumpter,J.P. The purification and partial characerization of carp, Cyprinus carpio, vitellogenin.(1990a). Fish Phsiology and Biochemistry 8, 111-120. Tyler,C.R., and Sumpter,J.P. The development of a radioimmunoassay for carp , Cyprinus carpio, vitellogenin.(1990b). Fish Phyiology and Biochemistry 8,129-140. Tyler,C.R., Sumpter,J.P., and B.van der Eerden. Measurement of vitellogenin, a biomarker for exposure to oestrogenic chemicals, in a wide varitey of cyprinid fish.(1996). J.Comp.Physiol. B 166, 418-426. Wahli,W., Dawid,I.B., Ryffel,G.U., and Weber,R. (1981). Vitellogenesis and the vitellogenin gene family. Science 212, 298-304. Wall,D.A. and Patel,S. (1987). The intracellular fate of vitellogenin in Xenopus oocytes is determined by its extracellular concentration during endocytosis. J. Biol.Chem. 262, 14779-14789. Wallace,R.A. and Begovac,P.C. (1985). Phosvitins in Fundulus oocytes and eggs. Preliminary chromatographic and electrophoretic analyses together with biological considerations. J. Biol.Chem. 260, 11268-11274. Yamamura,J., Adachi,T., Aoki,N., Nakajima,H., Nakamura,R., and Matsuda,T. (1995). Precursor-product relationship between chicken vitellogenin and the yolk proteins: the 40 kDa yolk plasma glycoprotein is derived from the C-terminal cysteine-rich domain of vitellogenin II. Biochim. Biophys. Acta 1244, 384-394. Yoshizaki,N., Moriyama,A., and Yonezawa,S. (1998). Purification and properties of embryonic cysteine proteinase which participates in yolk-lysis of Xenopus laevis. Comp Biochem. Physiol B Biochem. Mol. Biol.119, 571-576. Yoshizaki,N. and Yonezawa,S. (1998). Cysteine proteinase plays a key role for the initiation of yolk digestion during development of Xenopus laevis. Dev. Growth Differ. 40, 659-667. 曾登裕( 1996)：黑鯛卵黃前質基因cDNA部份序列之分析。國立臺灣大學漁業科學研究所碩士論文。 陳淑華(1993)：鯔魚卵黃前質及性類固醇結合蛋白之特性分析。台灣大學漁業科學研究所碩士論文。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75153 | - |
| dc.description.abstract | 卵膜是水生卵生動物很重要的構造，它防護卵細胞、受精卵及胚胎不遭受水生環境的各種物理化學性損害，因此它的存在有著極重要的生理意義。對於卵膜的組成，尚未完全被研究清楚，本實驗室先前對於鯉魚卵膜的研究已發現許多組成分子，包括ZP2、ZP3、cystaitin、cathepsin S like substance、fibroin like substance、FEO-1，並對其性狀加以探討。然而卵膜上仍有許多未被發現的組成分子，本篇論文的主要目的即在鑑識鯉魚卵膜萃取液中分子量為 25kDa 及 30kDa 之組成分子。 本論文首先從鯉魚的卵膜萃取液中，純化出 p25 及 p30，藉由胺基端的胺基酸定序，發現胺基端序列和鯉魚的卵黃生成前質素的羧基端極為類似，藉由進一步的基因還殖與比對其他物種相似的胺基酸區域，我們確定p25 為卵黃生成前質素的lipovitellin II 部分。經由使用免疫組織化學的方法觀察 p25 在鯉魚卵細胞的分佈位置，發現在表層反應前的卵膜上即有 p25 的存在，而在表層反應後， p25在卵膜的量明顯地增加。至於 p25 是如何釋放到卵膜上，其機制還需更進一步的探討。 此外我們也將卵黃生成前質素的全長基因還殖出來，目前已完成 3500bp 的定序，此基因將可作為日後研究除了 lipovitellin Ⅱ 外，是否還有其他部分的卵黃生成前質素組成卵膜蛋白之用。 | zh_TW |
| dc.description.abstract | In aquatic oviparous animals, the chorion is a critical structure. It protects the fertilized egg and early embryo from chemical and physical damage. The knowledge about the components of piscine chorion is still limited. In our preliminary study on the components of carp chorion, some components have been identified, including ZP2、ZP3、cystatin、cathepsin S-like substance、fibroin-like substance and FEO-l. However, there are still a lot of unidentified chorion proteins. In this study, the two components, p25 and p30, from carp chorion are found. A series of studies are performed to characterize them. The N-terminal amino acid sequence of p25 is very similar to the partial sequence of fathead minnow vitellogenin. By 3'-RACE, the p25 cDNA was cloned and analyzed. The deduced amino acid sequence of p25 cDNA is also homologous to lipovitellin II that is located at the C-terminal of the vitellogenin from various vertebrates. The result of immunocytochemical staining reveals that the p25 is present in the carp chorion before ovulation. Moreover, the signal intensity of p25 in the chorion of cortical reacted eggs is higher than that of oocytes and ovulated eggs. The mechanism of translocation of p25 to the chorion remains to be elucidated. A cDNA of carp vitellogenin of 3.5kb is cloned. The cDNA may provide further information regarding whether other parts of vitellogenin may or may not be a component of carp chorion. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2000 | en |
| dc.description.tableofcontents | 中文摘要.........................................................i 英文摘要........................................................ii 壹、緒論.........................................................1 貳、材料與方法...............................................8 參、結果.....................................................28 肆、討論.....................................................36 伍、參考文獻................................................42 陸、圖表.....................................................48 | |
| dc.language.iso | zh-TW | |
| dc.title | Lipovitellin Ⅱ為鯉魚卵膜組成之鑑定與其前質－卵黃生成前質素基因之選殖 | zh_TW |
| dc.title | Identification of Lipovitellin as a Component of Carp Chorion and Cloning of Its Precursor, Vitellogenin | en |
| dc.date.schoolyear | 88-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 76 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
文件中的檔案：
沒有與此文件相關的檔案。
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。