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DC 欄位 | 值 | 語言 |
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dc.contributor.author | Wang-Jing Liu | en |
dc.contributor.author | 劉宛菁 | zh_TW |
dc.date.accessioned | 2021-07-01T08:11:58Z | - |
dc.date.available | 2021-07-01T08:11:58Z | - |
dc.date.issued | 2000 | |
dc.identifier.citation | Ahrens, C. H., Russell, R. L. Q., Funk, C. J., Evans, J. T., Harwood, S. H. & Rohrmann, G. F. (1997). The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome. Virology 229, 381-399. Baylis, S. A., Banham, A. H., Vydelingum S., Dixon, L. K. & Smith, G. L. (1993). African swine fever virus encodes a serine protein kinase which is packaged into virions. Journal of Virology 67, 4549-4556. Bischoff, D. S. & Slavicek, J. M. (1994). Identification and characterization of a protein kinase gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus. Journal of Virology 68, 1728-1736. Blanchet-Tournier, M. F., Tricoire, H., Busson, D. & Lamour-Isnard, C. (1995). The segment-polarity gene fused is high conserved in Drosophila. Gene 161, 157-162. Blenis, J. & Erikson, R. L. (1984). Phosphorylation of the ribosomal protein S6 is elevated in cells transformed by a variety of tumor viruses. Journal of Virology 50, 966-969. Blissard, G. W. & Rohrmann, G. F. (1989). Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein gene of the Orgyia pseudotsugata multicapsid nulear polyhedrosis virus. Virology 170, 537-555. Chang, P. S., Lo, C. F., Wang, Y. C. & Kou, G. H. (1996). Identification of white spot syndrome associated baculovirus (WSBV) target organs in shrimp, Penaeus monodon by in situ hybridization. Diseases of Aquatic Organisms 27, 131-139. Chou, H.-Y., Huang, C.-Y., Wang, C.-H, Chiang, H.-C. & Lo, C.-F. (1995). Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Diseases of Aquatic Organisms 23, 165-173. Edelman, A. M., Blumenthal, D. K., & Krebs E. G. (1987). Protein serine/ threonine kinases. Annual Review Biochemistry 56, 567-631. Flegel, T. W. (1997). Special topic review: Major viral diseases of the black tiger prawn (Penaeus monodon) in Thailand. World Journal of Microbiology and Biotechnology 13, 433-422. Frame, M. C., Purves, F. C., McGeoch, D. J., Marsden, H. S. & Leader, D. P. (1987). Identification of the herpes simplex virus protein kinase as the product of the viral gene US3. Journal of General Virology 68, 2699-2704. Friesen, P. D. & Miller, L. K. (1985). Temporal regulation of baculovirus RNA: overlapping early and late transcripts. Journal of Virology 54, 392-400. Gomi, S., Majima, K. & Maeda, S. (1999). Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus. Journal of General Virology 72, 2645-2651. Hank, S. K., Quinn, A. M. & Hunter, T. (1988). The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241, 42-52. Hank, S. K. & Hunter, T. (1995). The eukaryotic protein kinase superfamily: kinase (catalytic) domain strcture and classification. FASEB Journal. 9, 576-596. Johnson, L. N., Noble, M. E. M. & Owen, D. J. (1996). Active and inactive protein kinases: structure basis for regulation. Cell 85, 149-158. Kaerleiv, M. & Horak, I. (1978). Identification and characterization of ribosomal proteins phosphorylated in vaccinia virus-infected Hela cells. European Journal Biochemistry 90, 463-469. Kawaguchi, Y., Sant, C. V. & Roizman, B. (1998). Eukaryotic elogation factor 1δ is hyperphosphorylated by the protein kinase encoded by the UL13 gene of Herpes Simplex virus 1. Journal of Virology 72, 1731-1736. Kelly, D. C. & Lescott, T. (1984). Baculovirus replication: phosphorylation of polypeptides synthesized in Trichoplusia ni nuclear polyhedrosis virus-infected cells. Journal of General Virology 65, 1183-1191. Kozak, M. (1989). The scanning model for translaton: an update. The Journal of cell Biology 108, 229-241. Kozak, M. (1995). Adherence to the first-AUG rule when a second AUG codin follows closely upon the first. Proceedings of the National Academy Science. USA 92, 2662-2666. Kuzio, J., Pearson, M. N., Harwood, S. H., Funk, C. J., Evans, J. T., Slavicek, J. M. & Rohrmann, G. F. (1999). Sequence and analysis of the genome of a baculovirus pathogenic for Lymantria dispar. Virology 253, 17-34. Lamb, R. A. & Choppin, P. W. (1977). The synthesis of Sendai virus polypeptides in infected cells. III. Phosphorylation of polypeptides. Virology 81, 382-397. Leader, D. P. & Katan, M. (1988). Viral aspects of protein phosphorylation. Journal of General Virology 69, 1441-1464. Lightner, D. V. (ed) (1996). A handbook of pathology and diagnostic procedures for diseases of penaeid shrimp. Section 2, pp. 3. World Aquaculture Society, Baton Rouge, LA, USA. Lo, C.-F., Leu, J.-H., Ho, C.-H., Chen, C.-H., Peng, S.-E., Chen, Y.-T., Chou, C.-M., Yeh, P.-Y., Huang, C.-J., Chou, H.-Y., Wang, C.-H. & Kou, G.-H. (1996 a). Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction. Diseases of Aquatic Organisms 25, 133-141. Lo, C.-F., Ho, C.-H., Peng, S.-E., Chen, C.-H., Hsu, H.-C., Chiu, Y.-L., Chang, C.-F., Liu, K.-F., Su, M.-S., Wang, C.-H. & Kou, G.-H. (1996 b). White spot syndrome baculovirus (WSBV) detected in cultured and captured shrimp, crab and other arthopods. Diseases of Aquatic Organisms 27, 215-225. Lo, C.-F., Ho, C.-H., Chen, C.-H., Liu, K.-F., Chiu, Y.-L., Yeh, P.-Y., Peng, S.-E., Hsu, H.-C., Liu, H.-C., Chang, C.-F., Su, M.-S., Wang, C.-H. & Kou, G.-H. (1997 a). Detection and tissue tropism of white spot syndrome baculovirus (WSBV) in captured brooders of Penaeus monodon with a special emphasis on reproductive organs. Diseases of Aquatic Organisms 30, 53-72. Lo, C.-F., Wang, C.-H. & Kou, G.-H. (1997 b). White spot syndrome (WSS): pathology, hosts and prevalence in captured shrimp and crabs in Taiwan. In New approaches to viral diseases of aquatic animals. NRIA International workshop proceedings, National Research Institute of Aquaculture, Mie, Japan, pp. 206-217. Edited by Inui, Y. Lo, C.-F, Hsu. H.-C., Tsai, M.-F., Ho, C.-H., Peng, S.-E., Kou, G.-H. & Lightner, D. V. (1999). Specific genomic DNA fragment analysis of different geographical clinical samples of shrimp white spot syndrome virus. Diseases of Aquatic Organisms 35, 175-185. Mainprize, T. H., Lee, K. & Miller, L. K. (1986). Variation in the temporal expression of overlapping baculovirus transcripts. Virus Research 6, 85-99. Martin-Perez, J., Siegmann, M. & Thomas, G. (1984). EGF, PDGF, and insulin induce the phosphorylation of identical S6 peptides in Swiss mouse 3T3 cells: Effect of cAMP on early sites of phosphorylaton. Cell 36, 287-294. Maruniak, J. E. & Summers, M. D. (1981). Autographa californica nuclear polyhedrosis virus phosphoproteins and synthesis of intracellular proteins after virus infection. Virology 109, 25-34. Miller, L. K., Adang, M. J. & Browne, D. (1983). Protein kinase activity associated with extracellular and occluded forms of the baculovirus Autographa californica nuclear polyhedrosis virus. Journal of Virology 46, 275-278. Morrison, E. E., Wang, Y.-F. & Meredith D. M. (1998). Phosphorylation of structure components promotes dissociation of the Herpes Simplex Virus Type 1 tegument. Journal of Virology 72, 7108-7114. Murphy, F. A., Fauquet, C. M., Bishop, D. H. L., Ghabrial, S. A., Jarvis, A. W., Martelli, G. P. & Summers, M. D. (1995a). Classification and nomenclature of viruses. Arch Virol, suppl 10, 1-586. Murphy, F. A., Fauquet, C. M., Bishop, D. H. L., Ghabrial, S. A., Jarvis, A. W., Martelli, G. P. Mayo, M. A. & Summers, M. D. (1995b). Classification and nomenclature of viruses: sixth report of the International Committee on Taxonomy of Viruses. Springer-Verlag, New York. Nakano, H., Koube, H., Umezaea, S., Momoyama, K., Hiraoka, M., Inouye, K. & Oseko N. (1994). Mass mortalities of cultured kuruma shrimp, Penaeus japonicus, in Japan in 1993: epizootiological survey and infection trials. Fish Pathology. 29, 135-139 (in Japanese). Purves, F. C. & Roizman, B. (1992). The U 13 gene of herpes simplex virus 1 encodes the functions for posttranslational processing associated with phosphorylation of the regulatory protein α22. Proceedings of the National Academy Science. USA 89 7310-14. Purves, F. C., Ogle, W. O. & Roizman, B. (1993). Processing of the herpes simplex virus regulatory protein α22 mediated by UL13 protein kinase determines the accumulation of a subset of α and γ mRNA and proteins in infected cells. Proceedings of the National Academy Science. USA 90, 6701-6705. Quideng, Q. & Van Etten, J. L. (1995). Characterization of a protein kinase gene from two Chlorella virus. Virus Research 35, 291-305. Reese, M. G. & Eeckman, F. H. (1995). New neural network algorithms for improved eukaryotic promotor site recognition. In Genome sequence and Technology, Vol. 1. Editor by J. C. Venter and D.Doyle. Reilly, L. M. & Guarino, L. A. (1994). The pk1 gene of Autographa Californica multinucleocapsid nuclear polyhedrosis virus encodes a protein kinase. Journal of General Virology 75, 2999-3006. Sally, A. B., Alison, H. B., Soopayah, V., Linda, K. D. & Smith G. L. (1993). African swine fever virus encodes a serine protein kinase which is packaged into virions. Journal of Virology 67, 4549-4556. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory. Sefton, B., Hunter, T., Beemon, K. & Eckhart, W. (1980). Evidence that the phosphirylation of tyrosine is essential for cellular transformation. Cell 20, 807-816. Smith, R. F. & Smith, T. F. (1989). Identification of new protein kinase-related genes in three herpesviruses, Herpes Simplex virus, Varicella-Zoster virus, and Epstein-Barr virus. Journal of Virology 63, 450-455. Smith, G. E. & Summers, M. D. (1981). Application of a novel radioimmuoassay to identify baculovirus structural proteins that share interspecies antigenic determinants. Journal of Virology 39, 125-137. Somogy, T., Michelson, S. & Masse, M.-J. O. (1990). Genomic location of a human cytomegalovirus protein with protein kinase activity (K68). Virology 174, 276-285 Tilakaratne, N., Hardin, S. E. & Weaver, R F. (1991). Nucleotide sequence and transcript mapping of the HindIII F region of the Autographa californica nuclear polyhedrosis virus genome. Journal of General Viology 72, 285-291. Tiollais, P., Charney, P. & Vyas, G. N. (1981). Biology of Hepatitis B virus. Science (Washington, D. C.) 213, 406-411. Tsiapalis, C. M. (1977). Chemical modification of DNA polymerase phosphoprotein from avian myeloblastosis virus. Nature (Lodon) 266, 27-31. van Hulten, M. C. W., Westenberg, M., Goodall, S. D. & Vlak, J. M. (2000). Identification of two major virion protein genes of white spot syndrome virus of shrimp. Virology 266, 227-236. van Hulten, M. C. W., Tasi M.-F., Schipper C. A., Lo, C.-F., Kou, G.-H. & Vlak J. M. (2000). Analysis of a genomic segment of white spot syndrome virus of shrimp containing ribonucleotide reductase genes and repeat regions. Journal of General Viology 81, 307-316. Wang, C.-H., Lo, C.-F., Leu, J.-H., Chou, C.-M., Yeh, P.-Y., Chou, H.-Y., Tung, M.-C., Chang, C.-F., Su, M.-S. & Kou, G.-H. (1995). Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon. Diseases of Aquatic Organisms 23, 239-242. Witt, D. J., Naeve, C. W. & Summer, D. F. (1981). Phosphorylation of vesicular stomatitis virus proteins as a possible contributing factor in virion uncoating. Journal of General Viology 56, 383-391. Wilson, M. E. & Consigli, R. A. (1985). Characterization of a protein kinase activity associated with purified capsids of the Granulosis virus infecting Plodia interpunctella. Virology 143, 516-525. Yonghong, L. & Miller, L. K. (1995). Expression and analysis of a baculovirus gene encoding a truncated protein kinase homology. Virology 206, 314-323. Zemskov, E. A., Abramova, E. B. & Mikhailov, V. S. (1992). Induction of a new protein kinase in pupae of the silkworm Bombyx mori after infection with nuclear polyhedrosis virus. Journal of General Viology 73, 3231-3234. Zemskov, E. A., Abramova, E. B. & Mikhailov, V. S. (1991). Virus-induced phosphoproteins in silkworm pupae infected with nuclear polyhedrosis virus. Biokhimiya 56, 2207-2217. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75141 | - |
dc.description.abstract | 本論文以蝦白點症病毒(WSSV)長3.4kb的基因體片段進行序列分析及基因鑑定。此片段含有五個開放譯讀區(open reading frames,ORFs),其中ORF1與蛋白激?(protein kinase,PK)酵素作用區(catalytic domain)有很高的相似性。ORF2可轉譯出含204個胺基酸的蛋白質,為已知的蝦白點症病毒膜蛋白VP28。ORF3、ORF4和ORF5分別可轉譯出193、200及229個胺基酸的蛋白質。利用反轉錄聚合?鏈反應進行基因轉錄時序性分析,結果可知ORF1、VP28及ORF3為早期表現的基因,ORF4及ORF5則屬於晚期表現的基因。由於pk基因在病毒的致病力上扮演重要的角色,因此本研究針對蝦白點症病毒pk1基因進行選殖、定序及其特性分析。經由北方雜合反應結果顯示ORF1之轉錄子為2.7kb,而ORF1在3.4kb的片段上僅1020個核?酸長,因此首先對蝦白點症病毒pk1完整基因進行選殖。經選殖及鑑定後可知蝦白點症病毒pk1基因全長共2193個核?酸序列,可轉譯出730個胺基酸,分子量為81kDa,由胺基酸序列特性分析可知pk1基因為serine/threonine的蛋白激?。蝦白點症病毒PK1胺基酸序列含有蛋白激?所特有之保守性區域,subdomain I與II與其他物種進行比對其相似度介於24%?41%,subdomain VI其相似度介於69%?92%之間,subdomain VII相似度介於44%?77%之間,subdomain VIII相似度介於45%?63%之間及subdomain IX相似度介於21%?51%之間。分析蝦白點症病毒pk1基因結構可發現兩個TATA box分別位於轉譯起點上游80至75及19至14個核?酸的位置,而在3'端具有加聚嘌呤信號(AATAAA,poly A signal)。經試管內轉錄及轉譯分析結果顯示蝦白點症病毒PK1分子量為87kDa與電腦預測值相近,並且試管內所得之87 kDa產物可被抗蝦白點症病毒PK1重組蛋白抗血清所辨認。 | zh_TW |
dc.description.abstract | In the present study, a 3.4 kbp DNA fragment from white spot syndrome virus (WSSV) genomic HindIII library was studied. Following DNA sequencing and computer program prediction, five open reading frames (ORFs) were identified in this fragment. ORF1 has a high similarity to the protein kinase (PK) catalytic domain. ORF2 is identical to a previously reported WSSV envelope protein (VP28). Orf3, orf4 and orf5 potential encode for polypeptides of 193, 200 and 229 amino acids. Temporal analysis of the ORFs showed that ORF1, VP28 and ORF3 were first expressed at an early stage, but ORF4 and ORF5 were expressed at late stage. PK plays an important role in the pathogenicity of viruses, so this study focused on the cloning, sequencing, and characterization of WSSV pk1 gene. Northern blot analysis with an orf1-specific riboprobe detected a major transcript of 2.7 kb, which indicated that the 3.4 kbp fragment contained only part of WSSV pk1 gene. The full length WSSV pk1 gene was found in an 8421bp fragment from an EcoRI library. It contains 2193 nucleotides and encodes an 81-kDa protein of 730 amino acids. WSSV PK1 contains serine-threonine protein kinase consensus motifs I through XI. Compared to the PKs of other species, amino acid similarities are 24?41% for subdomains I and II; 69?92% for subdomain VI; 44?77% for subdomain VII; 45?63% for subdomain VIII; and 21?51% for subdomain IX. Analysis of WSSV pk1 gene structure indicated two TATA boxes at positions 75?80 and 14?19 nucleotides upstream of the translation start site, and a poly A signal (AATAAA) also located at the 3' terminal of the gene. Expression of WSSV PK1 with a rabbit reticulocyte generated an 87-kDa protein, which is in close agreement with the predicted size of 84-kDa from the amino acids sequence. This in vitro transcription and translation product was recognized by anti-WSSV PK1 recombinant protein antiserum. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2000 | en |
dc.description.tableofcontents | 前言 1 材料與方法 1.蝦白點症病毒基因體片段pmh12定序及開放譯讀區轉錄分析 9 1.1 核?酸序列定序及分析 9 1.2 蝦白點症病毒基因體片段pmh12開放譯讀區轉錄分析 9 1.2.1 實驗草蝦來源 9 1.2.2 病毒純化及實驗蝦體組織之收集 10 1.2.3 total RNA的萃取 10 1.2.4 反轉錄聚合?鏈反應(RT-PCR) 10 2.蝦白點症病毒蛋白激?完整基因之搜尋、定序及轉錄轉譯之分析 12 2.1 北方轉印雜合法(Northern blot hybridization) 12 2.1.1 riboprobe之製備 12 2.1.1.1 範本之製備 12 2.1.1.2 試管內轉錄反應(in vitro transcription) 13 2.1.2 北方轉印法(Northern blot)和北方雜合法(Northern hybridization) 13 2.2 快速放大cDNA基因(Rapid amplification of cDNA ends,RACE) 15 2.3 病毒純化及基因體DNA之萃取 16 2.3.1 病毒純化 16 2.3.2 病毒基因體DNA之萃取 16 2.4 南氏轉印雜合法(Southern blot hybridization) 17 2.4.1 探針(probe)之製備 17 2.4.2 南氏轉印法 18 2.4.3 雜合反應及DIG標定探針檢測之方法 18 2.5 蝦白點症病毒EcoRI基因庫之建立及蛋白激?基因DNA片段之選殖 19 2.6 大腸菌基因表現系統生產重組蛋白 20 2.7 抗蝦白點症病毒PK1重組蛋白抗體之製備 22 2.8 試管內轉錄和轉譯反應(in vitro transcription and translation) 22 3.蝦白點症病毒PK1胺基酸序列與其他物種蛋白激?序列之分析與比較 24 結果 1.蝦白點症病毒基因體片段pmh12定序及開放譯讀區轉錄分析 26 2.蝦白點症病毒蛋白激?完整基因之搜尋、定序及轉錄轉譯之分析 28 討論 32 參考文獻 41 圖表 51 附錄 68 | |
dc.language.iso | zh-TW | |
dc.title | 蝦白點症病毒蛋白激?基因之研究 | zh_TW |
dc.title | Study of the protein kinase gene of shrimp white spot syndrome virus | en |
dc.date.schoolyear | 88-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 76 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
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