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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chien-Ho Liu | en |
dc.contributor.author | 劉健合 | zh_TW |
dc.date.accessioned | 2021-07-01T08:11:57Z | - |
dc.date.available | 2021-07-01T08:11:57Z | - |
dc.date.issued | 2000 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75138 | - |
dc.description.abstract | 烏魚(Mugil cephalus L.)是台灣重要的經濟漁獲。台灣沿岸經年都有烏魚存在,而每年的冬至前後期間,亦有一群自北方洄游至台灣西南海域產卵的洄游性烏魚。本研究主要的目的在利用粒線體DNA(mtDNA)之控制區作為生化指標,藉以探討台灣沿岸與冬至洄游烏魚是否同為一個族群。本研究樣品共計烏魚43尾,其中包括新竹沿岸11尾與高雄沿岸10尾、冬至洄游必經海域(新竹外海)10尾與台灣西南海域產卵場(高雄外海)12尾。mtDNA經萃取後,利用特定的引子,以聚合?鏈鎖反應(PCR)方法,將mtDNA控制區放大並定序。其後,控制區序列以ClustalW軟體進行比對,發現台灣海域烏魚共可分為四種不同的單倍基因型。以Kimura雙參數模式(Kimura-2-parameter model)計算各基因型鹼基替代百分率,結果顯示台灣沿岸(包括新竹與高雄)的烏魚為同一族群,冬至洄游必經海域(新竹外海)的烏魚則為另一族群。而台灣西南海域產卵場所捕獲的烏魚,同時發現沿岸與冬至洄游的烏魚基因型。此結果指出,台灣西南海域可能同為沿岸與冬至洄游烏魚的產卵場,且兩個族群的產卵時間都在冬至前後。其次,為建立世界各海域烏魚單倍基因型親源關係樹,由Genbank取得大西洋西岸與夏威夷海域的烏魚控制區基因型共計100種,篩選出親源關係最遠的28種,合併台灣的4種,以淨核?酸替代數目作為遺傳距離,利用neighbor-joining方法建構全球烏魚單倍基因型親緣關係樹。結果顯示,已知烏魚在親緣關係上可分為大西洋與太平洋兩大支系。其中,太平洋支系內夏威夷與台灣海域的烏魚可明顯區分,而台灣海域亦可分為沿岸與冬季洄游兩個不同族群。近年來台灣烏魚的年漁獲量急速下降,本研究結果,可藉以釐清減產的烏魚究為沿岸抑或冬至洄游的族群,將有助於台灣烏魚資源的管理與評估。此外,針對烏魚苗放流,本研究所建訂之生化標籤可協助確定其基因型,以避免人工放流對原有生態之影響。而且冬至洄游烏魚基因型之確立,將有助於其棲息地與洄游路徑之研究。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2000 | en |
dc.description.tableofcontents | 目 次………………………………………………………………………………Ⅰ 摘 要………………………………………………………………………………Ⅲ 前言 1.烏魚簡介………………………………………………………………………1 2.粒線體DNA簡介…………………………………………………………………2 3.粒線體DNA與族群遺傳…………………………………………………………5 4.族群判定以mtDNA之控制區為主………………………………………………7 材料與方法 1.樣本採集與保存…………………………………………………………………9 2.DNA萃取…………………………………………………………………………9 3.控制區DNA定序…………………………………………………………………11 3.1.定序反應物之製備…………………………………………………………11 3.2.定序反應之進行……………………………………………………………13 4.資料分析………………………………………………………………………13 4.1 序列分析與單倍基因型親緣關係樹的重建………………………………13 4.2 以序列資料分析烏魚的族群結構…………………………………………14 4.2.1 族群內遺傳組成多型性的估算…………………………………………14 4.2.2 族群間分化程度的估算…………………………………………………16 結 果 1.烏魚的採樣……………………………………………………………………19 2.DNA引子的設計…………………………………………………………………19 3.粒線體DNA控制區序列定序的結果……………………………………………20 4.烏魚mtDNA控制區的單倍基因型………………………………………………21 5.烏魚mtDNA之D-loop鹼基的變異………………………………………………22 6.台灣海域烏魚單倍基因型組成以及遺傳多型性分析………………………23 7.世界各海域烏魚單倍基因型親緣關係樹的重建……………………………24 討論 1.粒線體DNA之D-loop與族群的判定……………………………………………26 2.烏魚粒線體控制區功能序列與其他動物的比較……………………………27 3.台灣烏魚族群單倍基因型之探討……………………………………………28 4.台灣烏魚族群的結構…………………………………………………………29 5.全球烏魚族群間遺傳變異的探討……………………………………………30 6.結語……………………………………………………………………………31 參考文獻……………………………………………………………………………34 圖表與說明…………………………………………………………………………38 圖……………………………………………………………………………………39 表……………………………………………………………………………………53 Appendix……………………………………………………………………………60 | |
dc.language.iso | zh-TW | |
dc.title | 台灣地區烏魚粒線體DNA控制區序列變異及族群遺傳之研究 | zh_TW |
dc.title | Variation of the mtDNA Control Region and Population Genetics of the Grey mullet (Mugil cephalus) in Taiwan | en |
dc.date.schoolyear | 88-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 78 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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