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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Yi-Hsueh Li | en |
| dc.contributor.author | 李宜學 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:11:55Z | - |
| dc.date.available | 2021-07-01T08:11:55Z | - |
| dc.date.issued | 2000 | |
| dc.identifier.citation | 黃建豪．1999．蝴蝶蘭兩種重複性DNA序列的分離與定性．國立臺灣大學植物科學研究所碩士論文。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75126 | - |
| dc.description.abstract | 微衛星體序列是以1-6 bp為組成單位的簡單重複性序列，普遍存在於真核生物的基因組中。本實驗以六種人工合成的微衛星體序列為探針，與七種蝴蝶蘭原生種Phalaenopsis amabilis var. formosa、P. stuartiana、P. equestris、P. lueddemanniana、P. amboinensis、P. violacea、P. mannii及一種朵麗蘭Doritis pulcherrima基因組DNA進行南方氏雜交和螢光原位雜交。南方氏雜交的結果，除(CAC)7序列外，其餘五種序列(AT)10、(CA)11、(GA)11、(GAA)7與(GATA)6，普遍存在於八種蝴蝶蘭的基因組中，且前三種序列的含量較多。螢光原位雜交的結果，(AT)10與(CA)11序列是屬於散佈型的重複性序列；(GA)11序列在P. amboinensis與P. violacea亦屬於散佈型，而在其餘六種蝴蝶蘭訊號集中於染色體中節處，是屬於聚集型。 | zh_TW |
| dc.description.abstract | Microsatellites consisting of tandemly repeated arrays of 1-6 nucleotide motif are ubiquitous in most eukaryotic genomes. In this study six microsatellites were used to investigate the physical distribution among seven Phalaenopsis species and the closely related species Doritis pulcherrima. Signals of various strength were detected in all tested plant genome as hybridized to motifs (AT)10, (CA)11, (GA)11, (GAA)7 and (GATA)6 but not (CAC)7. The motifs (AT)10, (CA)11 and (GA)11 were selected for examining physical distribution with fluorescence in situ hybridization because strong signals were resulted from them. It revealed that (AT)10 and (CA)11 were widely dispersed on all chromosomes of eight orchids. (GA)11 was also dispersed on chromosomes of P. amboinensis and P. violacea, but signals were mainly restricted to centromeric regions in other six orchids. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2000 | en |
| dc.description.tableofcontents | 中文摘要……………………………………………………i 英文摘要……………………………………………………ii 簡寫字對照表……………………………………………………iii 壹、前言……………………………………………………1 貳、材料與方法 一、植物材料……………………………………………………5 二、微衛星體序列探針的製備……………………………………………………5 三、南方氏雜交……………………………………………………7 四、螢光原位雜交……………………………………………………10 參、結果 一、南方氏雜交分析……………………………………………………16 二、螢光原位雜交的結果……………………………………………………21 肆、討論……………………………………………………32 伍、參考文獻……………………………………………………36 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用螢光原位雜交探討微衛星體序列在蝴蝶蘭的實質分佈 | zh_TW |
| dc.title | Investigation on the physical distribution of microsatellites in Phalaenopsis orchids by fluorescence in situ hybridization | en |
| dc.date.schoolyear | 88-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 40 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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