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The diversity of intraspecific genetics and reproduction of Sclerocystis sinuosa (Glomales)
|Publication Year :||2000|
|Abstract:||囊叢枝菌根菌之分類主要依其產生之無性孢子形態，因此常有混淆。台灣產波紋硬囊實果菌Sclerocystis sinuosa會產生兩種孢子果，兩種孢子果主要不同點在於包被的有無，因兩種之其他外型差異不大，引起此兩種孢子果是否為同種之懷疑。本論文則針對此菌種之生物多樣性，藉由掃描式電子顯微鏡、形態連續觀察及18S ribosomal DNA序列比較等方法來研究，試求來解決此問題。|
在以砂耕法多孢接種後，確實發現純種菌秣能產生兩種孢子果，且兩種孢子果的所佔比例會隨時間消長；若接種本菌株中無包被的孢子果較易產生兩型孢子果，而接種有包被之孢子果則產生無包被孢子果的比例較低。在繁殖菌種時也發現似乎是兩種孢子果的中間型，經掃瞄式電子顯微鏡觀察發現裸露孢子果被菌絲逐漸包裹，而形成包被。將本菌株無包被與有包被兩型孢子果之18S ribosomal DNA序列比對，發現此兩種不同表型之菌株DNA序列相同；再與其他已發表之菌種比對，與Glomus sinuosum具最高相似性，但仍有不同的鹼基。故認為會產生兩型孢子果之台灣產S. sinuosa與G. sinuosum系屬同一種，但與國外已發表之菌株為不同品系。
Arbuscular mycorrhizal fungi (AMF) are one of the most widespread group of fungi on earth. Taxonomists have described three families, six genera and approx. 150 species. This taxonomy is based on morphological characters, and especially on features of asexual spores. These characteristics may be difficult to discern and are subjected to alterations during spore ontogeny or by parasitism. And the morphological similarity might conceal physiological diversity in these fungi.
There are two kinds of sporocarp of Sclerocystis sinuosa collected from Taiwan. The size and shape of two sporocarp types are similar; however, they differ in the presence or lacking of sporocarp peridium. The present investigation employed 1 8S ribosomal DNA sequences, sporocarp ontogeny and pot inoculation test to explore their identity.
Numbers and ratios of sporocarp with different mode of peridium formation were varied according to time change after inoculation either by naked sporocarps or sporocarps with peridium. Two types of sporocarps were reproduced at maturity by inoculating solely naked sporocarps in root of sorghum seedlings in the sand pot culture. In contrast, only sporocarps covered peridium were reproduced solely at maturity by inoculating with peridial sporocarps in the same host and environment.
As the result of 1 8S ribosomal DNA sequence, there is no difference between two sporocarp types. But slight variation in sequence compared with Glomus sinuosum were observed. The two sporocarp types of S. sinuosa collected from Taiwan are thus identified as the same species with G. sinuosum, but can be treated as a different strain.
|Appears in Collections:||植物科學研究所|
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