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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Dai-Chang Kuo | en |
| dc.contributor.author | 郭大璋 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:11:49Z | - |
| dc.date.available | 2021-07-01T08:11:49Z | - |
| dc.date.issued | 2000 | |
| dc.identifier.citation | Ando, H., Adachi, M., Umeda, K., Matsuura, A., Nonaka, M., Uchio, R., Tanaka, H., and Motoki, M. (1989) Purification and characteristics of a novel transglutaminase derived from microorganism. Agric. Biol. Chem., 53(10): 2613-2617. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. (1987) Current protocols in molecular biology.3(superscript rd) Ed. Vol. 1:4.9.1- 4.9.4. John Wiley & Sons, Inc., New York. Bishop, P. D., Teller, D. C., Smith, R. A., Lasser, G. W., Gilbert, T., and Seale, R. L. (1990) Expression, purification, and characterization of human factor XIII in Saccharomyces cerevisiae. Biochemistry. 29: 1861-1869. Carlson, M and Botstein, D. (1982) Two differentially regulated mRNAs with different 5' ends encode secreted and intracellular forms of yeast invertase. Cell 28: 145-154. Chang, S. K. and Chung, S. I. (1986) Cellular transglutaminase. The particulate- associated transglutaminase from chondrosarcoma and liver: partial purification and characterization. J. Biol. Chem. 261: 8112-8121. Chen, D. C., Yang, B. C., and Kuo, T. T. (1992) One-step transformation of yeast in stationary phase. Curr. Genet. 21: 83-84. Falcone, P., Serafini-Fracassini, D., and Del Duca, S. (1993) Comparative studies of transglutaminase activity and substrates in different organs of Helianthus tuberosus. J. Plant Physiol. 142: 263-273. Folk, J. E. and Cole, P. W. (1965) Structural requirements of specific substrates for guinea pig liver transglutaminase. .1 Biol. Chem., 240(7): 2951-2960. Folk, J. E. and Cole, P. W. (1966a) Mechanism of action of guinea pig liver transglutaminase. J. Biol. Chem., 241(23): 55 18-5525. Folk, J. E. and Cole, P. W. (1966b) Transglutaminase: mechanistic features of the active site as determined by kinetic and inhibitor studies. Biochem. Biophys. Acta., 122: 244-264. Folk, J. E. (1980) Transglutaminase. Annu. Rev. Biochem., 49: 517-531. Haroon, Z. A., Hettasch, J. M., Lai, T.-S., Dewhirst, M. W., and Greenberg, C. S.(1999) Tissue transglutaminase is expressed, active, and directly involved in rat dermal wound healing and angiogenesis. FASEBJ. 13: 1787-1795. Ho, K.-C., Quarmby, V. E., French, F. S., and Wilson, E. M. (1992) Molecular cloning of rat prostate transglutaminase complementary DNA. J. Biol. Chem., 267(8):12660-12667. Icekson, I. and Apelbaum, A. (1987) Evidence for transglutaminase activity in plant tissue. Plant Physiol. 84: 972-974. Ikura, K., Kometani, T., Yoshikawa, M., Sasaki, R., and Chiba, H. (1980a) Crosslinking of casein components by transglutaminase. Agric. Biol. Chem. 44: 1567-1573. Ikura, K., Kometani, T., Yoshikawa, M., Sasaki, R., and Chiba, H. (1980b) Crosslinking of soybean 7S and 11 S proteins by transglutaminase. Agric. Biol. Chem. 44: 2979-2984. Jeon, W. M., Lee, K. N., Birckbichler, P. J., Conway, E., and Patterson, M. K. Jr. (1989) Colorimetric assay for cellular transglutaminase. Analytical Biolchem., 182: 170-175. Kawashima, S. (1991) Inhibition of rat liver transglutaminase by nucleotides. Experientia (Basel) 47 (7): 709-712. Kieffer, R, Wieser, H., Henderson, M. II., and Graveland, A. (1998) Correlations of the breadmaking performance of wheat flour with rheological measurements on a micro-scale. J. Cereal Sci. 27: 53-60. Kim, S. H., Carpenter, J. A., Lanier, T. C., and Wicker, L. (1993) Polymerizarion of beef actomyosin induced by transglutaminase. J. Food Sci., 58: 473-491. Kitabatake, N. and Doi, E. (1993) Improvement of protein gel by physical and enzymatic treatment. FoodRev. International, 9: 445-471. Klein, J. D., Guzman, E., and Kuehn, G. D. (1992) Purification and partial characterization of transglutaminase from physarum-polycephalum. Journal of Bacteriology 174 (8): 2599-2605. Lipmann, R. D. and Tuttle, L. C. (1944) A specific micromethod for the determination of acyl phosphates. J. Biol. Chem., 159: 21-28. Macreadie, I. G., Failla, P., Horaitis, O., and Azad, A. A. (1992). Production of HIV-1 VPU with pyeulcbx a convenient vector for the production of non-fused proteins in yeast. Biotechnology Letters, 14 (8): 63 9-642. Matheis, G. and Whitaker, J. R. (1987) A review: enzymatic cross-linking of proteins applicable to foods. J. Food Biochem., 11: 309-327. Motoki, M., Seguro, K., Nio, N., and Takinami, K. (1986) Glutamine-specific deamidation of α(subscript sl)-Casein by transglutaminase. Agric. Biol. Chem. 50(12): 3025- 3030. Nielsen, P. M. (1995) Reactions and potential industrial applications of transglutaminase. Review of literature and patents. Food Biotechnology, 9(3): 119-156. Nonaka, M., Tanaka, H., Okiyama, A., Motoki, M., Ando, H., Umeda, K., and Matsura, A. (1989) Polymerization of several proteins by Ca(superscript 2+)-independent transglutaminase derived from microorganism. Agric. Biol. Chem., 53: 2619-2623. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular cloning: a laboratory manual. 2(superscript nd) Ed. Cold Spring Harbor Laboratory Press, New York. Seguro, K., Kumazawa, Y., Ohtsuka, T., Toiguchi, S., and Motoki, M. (1995) Microbial transglutaminase and ε-(γ-glutamyl)lysine crooslink effects on elastic properties of kamaboko gels. J. Food Sci., 60(2): 305-311. Seiving, B., Stenberg, P., and Nilsson, B. (1991) A new assay for transglutaminase. Scand. J. Clin. Lab. Invest., 51: 119-124. Simmonds, D. H. (1989) Wheat and wheat quality in Australia. pp.183-213. Australian Wheat Board Press. Australia. Singh, H. (1991) Modification of food proteins by covalent crosslinking. Trends Food Sci. Technol., 2: 196-200. Wilson, E. M. and French, F. S. (1980) Biochemical homology between rat dorsal prostate and coagulating gland. Purification of a major androgen-induced protein. J. Biol. Chem., 255: 10946-10953. Wong, W. S. D., Batt, C., and Kinsella, J. E. (1990) Purification and characterization of rat liver transglutaminase. Int. J. Biochem. 22 (1). 1990. 53-60. Yasueda, H, Kumazawa, Y., and Motoki, M. (1994) Purification and characterization of a tissue-type transglutaminase from Red Sea bream (Pagrus major). Biosci. Biotechnol. Biochem., 58: 2041-2045. Zhu, Y., Rinzema, A., Tramper, J., and BoI, J. (1995) Microbial transglutaminase-a review of its production and application in food processing. Appl. Microbiol. Biotechnol., 44: 277-282. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75101 | - |
| dc.description.abstract | 穀胺酸轉氨?（蛋白質－穀胺醯酸γ-穀胺醯基轉移?，EC2.3.2.13）可催化醯基轉移反應，將兩個蛋白質或勝?經由穀胺醯酸與穀胺醯酸或穀胺醯酸與離胺酸的鍵結而連結起來。我們利用小鼠攝護腺cDNA基因庫中篩選出的小鼠攝護腺穀胺酸轉氨?基因為範本，DPSAC-1及DPSAC-2為引子 (Primer)進行聚合?連鎖反應，將所得之基因片段構築於表現載體pYEX－Sl上，並轉殖進入酵母菌(Saccharomyces cerevisiae) BJ2168中表現。由北方雜合法(Northern Blotting)證實小鼠攝護腺穀胺酸轉氨?基因在酵母菌中能進行轉錄作用，並產生一個約60kDa的小鼠攝護腺穀胺酸轉氨?蛋白。利用hydroxamate的生成反應，計算出穀胺酸轉氨?之k(上標 m)為19.65mM，?之比活性(specific activity)為8.08?mole/min/mg。每公升酵母菌可生產0.9mg的穀胺酸轉氨?。由於穀胺酸轉氨?在食品加工上可改良食品的品質，我們將酵母菌含有穀胺酸轉氨?的培養基上清液加入麵糰中，可使麵糰的抗延展力增加及延展性降低，而改善麵糰的品質。 | zh_TW |
| dc.description.abstract | Transglutaminase (protein-glutamine γ-glutaminyltransferase, EC 2.3.2.13) is an enzyme capable of catalyzing acyl-transfer reactions by introducing covalent cross-links between proteins or peptides via peptide bond formation between glutamine and glutamine or glutamine and lysine. A cDNA encoding rat prostate transglutaminase was amplified by polymerase chain reaction (PCR) with DPSAC-1 and DPSAC-2 primers. The PCR product carrying the rat prostate transglutaminase gene was inserted into pYEX-S1, and expressed in Saccharomyces cerevisiae BJ2 168. The northern blot analysis indicated a RNA of 2-kb was transcribed in yeast, and a protein of about 60 kDa was expressed. The activity of transglutaminase was determined by hydroxamate assay. The k(subscript m) and specific activity of the enzyme was 19.65 mM and 8.08?mole/min/mg, respectively. Zero point nine milligrams of transglutaminase can be produced from one liter of culture medium. The ability of transglutaminase to improve the properties of foods was also examined. After transglutaminase treatment, the dough became less sticky and extensible, and more resistant extension. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2000 | en |
| dc.description.tableofcontents | 中文摘要………………………………………………………………………3 Abstract………………………………………………………………………4 Introduction………………………………………………………………5 Materials and Methods……………………………………………………10 Results…………………………………………………………20 Discussion……………………………………………………36 Reference…………………………………………………………40 | |
| dc.language.iso | zh-TW | |
| dc.title | 小鼠攝護腺穀胺酸轉氨?在酵母菌中的表現及其特性 | zh_TW |
| dc.title | Expression and Characterization of Rat Prostate Transglutaminase in Saccharomyces cerevisiae | en |
| dc.date.schoolyear | 88-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 45 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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