Please use this identifier to cite or link to this item:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75095Full metadata record
| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.author | 林秀芳 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:11:48Z | - |
| dc.date.available | 2021-07-01T08:11:48Z | - |
| dc.date.issued | 1980 | |
| dc.identifier.citation | 1. Auger-Buendia, M. A., K. Longuet, and A. Tavitian. 1979. Kinetics studies on ribosomal proteins assembly in preribosomal particles and ribosomal subunits of mammalian cells. Biochim. Biophys. Acta. 563:113-128. 2. Ballal, N. R. and H. Busch. 1974. The dynamic state of liver nucleolar proteins as reflected by their change during administration of thioacetamide. Life Sci. 14:1835-1845. 3. Brown, D. D. and J. B. Gurdon. 1964. Absence of ribosomal RNA synthesis in the anucleolate mutant of X. laevis. Proc. Natl. Acad. Sci. U. S. A. 51:139-146. 4. Brown, D. B. and I. B. Dawid. 1968. Specific gene amplification in oocytes. Science 160:272. 5. Burton, K. 1968. Determination of DNA concentration with diphenylamine. Methods Enzymol. 12B:165-166. 6. Busch, H. and K. Smetana. 1970. The Cell Nucleolus. Academic Press, New York. 7. Busch, H. and K. Smetana. 1974. The Cell Nucleus. Vol. 1 The Nucleolus and Nucleolar DNA. Academic Press, New York. pp. 75-77. 8. Chambon, P. 1975. Eukaryctic nuclear RNA polyrnerase. Ann. Rev. Biochem. 44:613-638. 9. Chen, Y. M., C. Y. Bin, H. Chang, T. J. Guilfoyle, and J. L. Key. 1975. Isolation and properties of nuclei from control and auxin-treated soybean hypocotyl. Plant Phsiol. 56:78-82. 10.Chen, Y. M., C. Y. Din, S. F. Lin, D. H. Huang, and J. L. Key. 1980. unplished data. 11. Gu1foyle, T. J. and J. B. Hanson. 1974. Greater length of riboncleic acid synthesized by chromatin bound polymerase from auxin-treated soybean hypoco tyls. Plant Physiol. 53:110-113. 12. Guilfoyle, T. J., C. Y. Lin, Y. N. Chen, R.T. Nagao, and J. L. Nev. 1975. Enhancement of soybean RNA polymerase I by auxin. Proc. Natl. Aced. Sci. U. S. A. 72:69-72. 13. Guilfoyle, T. J., N. Olszewski, arid L. L. Zurfluh. 1980. RNA polymerases and transcription during developmental transitions in soybean. In: Genome Organzation and Expression in Plants. (ed. by C. J. Leaver) , Plenum, Press. New York. in press. 14. Hardy, S. J. S., C. G. Kurland, P. Voynow, and G. Mora. 1969. The ribosomal proteins of E. coli. I. Purification of the 30 S ribosomal proteins. Biochemistry 8:2897-2905. 15. Howell, S. H. 1973. The isolation and analysis of DNA from eukaryotic cells. In: Molecular Techniques and Approaches in Developmental Biology. (ed.by M. J. Chrispeels). pp.117-139. 16. Hunag, D. H. 1979. Biochemical analysis of soybean nucleoli. M. S. thesis. Department of Botany, University of Georgia, U. S. A. 17. Ingle, J. and J. L. Key. 1965. A comparative evaluation of the synthesis of DNA-like RNA in excised and intact plant tissues. Plant Physiol. 40:1212-1219. 18. Ingle, J. and J. Sinclair. 1972. Ribosomal RNA genes and plant development. Nature, London 235:30-32. 19. Jacob, S. T., S. N. Sajdel, and H. N. Munro. 1970. Different response of the whole nuclear RNA polymerase and soluble nucleolar RNA polymerase to divalent cations and to inhibition by α-amanitin. Bic-chem. Biophys. Res. Commnu. 38:765-770. 20. Key, J. L., C.Y. Lin, E. M. Gifford, and R. Dangler. 1966. Re1aton of 2,4-D-induced growth aberration to chances in nucleic acid metabolism in soybean seedlings. Bot. Gaz. 127:87-94. 21. Leaver, C. J. and J. L. Key. 1970. Ribosomal RNA synthesis in plants. J. Mol. Biol. 49:671-680. 22. Lin, C. Y. and J. L. Key. 1971. Dissociation of N2-gas induced monomeric ribosomes and functioning of the derived subunits. Plant Physiol. 48:547-552. 23. Lin, C. Y., T. J. Guilfoyle, Y. M. Chen, and J. L. Key. 1975. Isolation of nucleoli and localization of ribonucleic acid polymerase I from soybean hypocotyl. Plant Physiol. 56:850-852. 24. Loening, U. E. 1969. The determination of the molecular weight of ribonucleic acid by polyacrylamidegel electrophoresis. Biochem. J. 113:131-138. 25. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 192:265-275. 26. McClintock, B. 1947. The relationship of a particular chromosomal element to the development of the nucleoli in Zea mays. Zeit. Zellforsch. Mik. Anat. 21:294. 27. Mets, L. and L. Bogorad. 1974. Two-dimensional polyacrylamide gel electrophoresis: an improved method for ribosomal proteins. Anal. Biochem. 57:200-210. 28. Payne, P. I. and U. E. Loening. 1970. RNA breakdown accompanying the isolation of pea root microsemes. Biochim. Biophys. Acta. 224:128-135. 29. Perry, R. P., T. Cheng, J. J. Fred, J. P. Greenberg, D. E. Kelly, and K. D. Tartof. 1970. 1970. Evolution of the transcription unit of ribosomal RNA. Proc. Nati. Aced. Sci. U. S. A. 65:609-616. 30.Prestayko, A. W., C. R. Klornp, D. J. Schrroll, and Natl. Acad. Sci. U. S. A. 65:609-616. 30 Prestayko, A. W., G. R. Klomp, D. J. Schmoll, and H. Busch. 1974. Comparison of proteins of ribosomal subunits and nucleolar preribosomal particles from Novikoff Hepatoma Ascites Cells by two-dimensional polyacrylamide gel electrophoresis. Biochemistry 13:1945-1951. 31. Price, R. and S. Penman. 1972. A distinct RNA polymerase activity, synthesizing 5.5 S, 5 S and 4 S in nuclei from adenovirus 2-infected HeLa cells. J. Mol. Biol. 70:435-450. 32. McConkey, B. H. arid J. W. Hopkins. 1964. The relationship of the nucleolus to the synthesis of rPNA in HeLa cells. Proc. Natl. Acad. Sci. U. S. A. 51: 1197-1204. 33. Reeder, R. H. and P. C. Roeder. 1972. Ribosomal RNA synthesis in isolated nuclei. J. Mol. Bid. 67:433-441. 34. Rizzo, P. J., K. Pedersen, and J. H. Cherry. 1977. Stimulation by a soluble factor isolated from soybean hypocotyl by 2, 4-D affinity chromatography. Plant Sci. Lett. 8(3):205-211. 35. Roeder, P. C. and W. J. Rutter. 1969. Multiple forms of RNA polymerase in eukaryotic organism. Nature (London) 224:234-237. 36. Poeder, R. G. 1976. Eukaryotic nuclear RNA polymerases. In: RNA polymerases. (eds. by N. Chamberlin and P. Losicks). Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 285-329. 37. Roeaer, M. E. and P. S. S. Froser. 1970. Ribosomal RNA precursor in plants. J. Mol. Bid. 49:681-692. 38. Schneider, W. C. 1957. Determination of nucleic acidby pentose analysis. Methods Enzymol. 3:680-684. 39.Takahashi, N., J. Shimada, J. Higo, S. Sigo, and by pentose analysis. Methods Enzymol. 3:680-684. 39. Takahashi, N., J. Shimada, J. Higo, S, Sigo, and S. Tanifugi. 1972. Ribosomal precursor particles from plant nucleoli. Biochim. Biophys. Acta. 262: 502-513. 40. Valenzuela, P., G. L. Hager, F. Weinberg, and W.J. Rutter. 1976. Molecular structure of yeast RNA polymerase I I I: Demonstration of the tripartite transeriptive system, in lower eukaryotes. Proc. Natl. Acad. Sci. U. S. A. 73(4):1024-1028. 41. Warner, J. R. and P. Soeiro. 1967. Nascent ribosomes from HeLa cells. Proc. Natl. Acad. Sci. U. S. A. 58:1984-1990. 42. Weinberg, R. A., U. S. Loening, M. Willems, and. S. Penman. 1967. Acrylamide gel electrophoresis of HeLa cell nucleolar PNA. Proc. Natl. Acad. Sdi. U. S. A. 58:1088-1095. 43. Weinberg, R. A. and S. Penman. 1970. Processing of 45 S nucleolar RNA. J. Mol. Biol. 47:169-178. 44. Weinmann, R. and P. G. Roeder. 1974. Role of DNA-dependent RNA polymerase I I I in the transcription of the tRNA and 5 S RNA genes. Proc. Natl. Acad. Sci. U. S. A. 71:1790-1794. 45. Zylber, E. A. and S. Penman. 1971. Products of RNA polymerase in HeLa cell nuclei. Proc. Natl. Acad. Sci. U. S. A. 68:2861-2865. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75095 | - |
| dc.description.abstract | 由2,4-D處理過之大豆幼苗下胚軸分離核仁，再利用30mM dithiothreitol使核仁瓦解而分離核仁染色質及先質核糖體。核仁內的RNA聚台?Ⅰ的活性佔總活性的90%以上，當把核仁瓦解分出核仁染色質及先質核糖體後，約有90%以上的RNA聚合?Ⅰ出觀在核仁染色質上，而先質核糖體部份只佔10%左右。但先質核糖體卻含有較高的RNA聚合?Ⅱ的活性。 核仁染色質的RNA與蛋白質的比例約為1:3；若以核仁的結DNA當 100，則核仁染色質的DNA約佔90%，而只有10％在先質核糖體部份。若把分離核仁染色質後的上清液用200,000×g將先質核糖體離心下來，則其RNA 與蛋白質的比值為1:1.13。將此先質核糖體經15-60%連續蔗糖濃度梯度離心後，發現其沉降係數約在80 S附近，與細胞質單核糖體類似。 試以2.4露膠體電泳分析核仁、核仁染色質與先質核糖體的RNA類型，並以細胞質80 S單核糖體之RNA作為對照標準。由結果知先質核糖體的rRNA類型與細胞質單核糖體類似，即主要為25 S及18 S二種rRNA所組成；但核仁染色質則除了有25 S及18 S RNA外，尚有大量的小分子RNA，這些少分子RNA所佔的比率比核仁大，由此可推知核仁染色質是這些小分子RNA存在的主要場所。 細胞質80 S單核糖體的鹼性蛋白質，在二度膠體電泳分析後，發現共有86個蛋白質點；而先質核糖體的鹼性蛋白質同樣做二度膠體電泳分析，共有77點，其中71點與核糖體蛋白質一樣。核仁染色質的鹼性蛋白質則共有54個，其中最主要的有9點；這9點經鑑定後是屬於組蛋白，而其約佔全部核仁染色質鹼性蛋白質80％以上。 | zh_TW |
| dc.description.abstract | Nucleoli isclated from auxin-treated soybean hpocotyl were fractionated into nucleclar chromatin and nucleolar preribosoral particles by suspending the nucleolar preparation in 30 mM DTT. Majority of RNA polymerase I activity in nucleoli was recevered in nucleolar chromatin fraction. Nucleolar chrocmatin also contained most of nucleolar DNA and RNA to protein ratio was 1:3. Only less than l0% of RNA polymerase I activity in nucleoli was found in nucleolar preribosonal fraction. Nucleolar preribosomal particles contained RNA to protein ratio of 1:1. When the nucleolar preribosomal particles were layered on 15-60% 1inear sucrcse gradient for centrifugation. they sedinented lighter than run-off 80 S single ribosorres. RNA species extracted from preribosomal particles were analyzed with 2.4% polvacrylamide gel using rRNAs from 80 S run-off side ribosomes as reference. The results showed that preribosomal particles mainly consisted of 25 S and 18 S rENA in a ratio of 1:1. However, RNA species isolated from nucleolar chrorratin consisted of low molecular weight RNAs besides 25 S and 18 S rNAS. Acid-soluble proteins associated with preribosomal particles were resolved into 77 protein spots on 2-D gel electrophoresis and 71 protein spots were presented in 80 S run-off ribosomes. They contributed core thar. 80% of total acid-soluble proteinss in nueleolar preribosoral particles. Acid-soiub1e proteins associated with nucleclar chromatin. were resolved into 54 protein spots and 9 of which were identified as histones. They constituted core than 80% of acid-soluble proteins in nucleolar chromatin. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:48Z (GMT). No. of bitstreams: 0 Previous issue date: 1980 | en |
| dc.description.tableofcontents | 英文摘要…………………………………1 中文摘要…………………………………3 一、緒言…………………………………5 二、實驗材料……………………………9 三、實驗方法……………………………12 四、實驗結果……………………………20 五、討論…………………………………50 六、參考文獻……………………………58 | |
| dc.language.iso | zh-TW | |
| dc.title | 大豆幼苗下胚軸核仁染色質與核仁先質核糖體的分離及其生化組成的研究 | zh_TW |
| dc.title | Fractionation of Nucleoli into Preribosomal Particles and Nucleolar Chromatin and their Biochemical Characterization | en |
| dc.date.schoolyear | 68-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 62 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| Appears in Collections: | 植物科學研究所 | |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.