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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | Chien-Hao Huang | en |
| dc.contributor.author | 黃建豪 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:11:33Z | - |
| dc.date.available | 2021-07-01T08:11:33Z | - |
| dc.date.issued | 1999 | |
| dc.identifier.citation | Appels, R., C. Driscoll, and W. J. Peacock. 1978. Heterochromatin and highly repeated DNA sequences in rye (Secale cereale) Chromosoma 70: 67-89.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75041 | - |
| dc.description.abstract | 我們從Phalaenopsis violacea的基因組DNA中分離出兩種重複性DNA序列，Pvr Ⅰ及Pvr Ⅱ，並分析了其核?酸序列、種間分佈及在染色體上的位置。PvrⅠ族重複性序列是由兩端富AT序列的區域及中間7 bp頭尾相接的重複性序列（GTAAGCC）13-22所組成。經南方氏雜交及螢光原位雜交得知，PvrⅠ族僅存在於含大染色體的P. mannii、P. amboinensis、P. venosa、P. violacea及Doritis pulcherrima的基因組中，且多聚集在大染色體末端AT含量豐富的異染色質區域。PvrⅡ族重複性序列則在八種原生種的基因組中皆存在，以選殖體Pvr-95為探針，經螢光原位雜交得知，此序列散佈於P. venosa的所有染色體中。 | zh_TW |
| dc.description.abstract | Two repetitive DNA sequences, Pvr Ⅰ and Pvr Ⅱ, were isolated from Phalaenopsis violacea and characterized. The Pvr Ⅰ family consists of AT-rich regions at 5' and 3' ends and 7 bp direct subrepeats (GTAAGCC)13-22. Southern analysis and fluorescence in situ hybridization (FISH) revealed that Pvr I is present in P. mannii, P. amboinensis, P. venosa, P. violacea and Doritis pulcherrima and mostly located in the AT-rich subtelomeric heterochromatin of large chromosomes. The Pvr Ⅱ family is present in all eight Phalaenopsis species studied. FISH using one clone (Pvr-95) as a probe showed that Pvr Ⅱ is dispersed on all chromosomes of P. venosa. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:33Z (GMT). No. of bitstreams: 0 Previous issue date: 1999 | en |
| dc.description.tableofcontents | 中文摘要……………………………………………………i 英文摘要……………………………………………………ii 壹、前言……………………………………………………1 貳、材料與方法 一、植物材料……………………………………………………6 二、DNA的抽取……………………………………………………6 三、探針的標定……………………………………………………8 四、高重複性選殖體的選殖……………………………………………………10 五、DNA序列的定序與分析……………………………………………………12 六、菌落雜交……………………………………………………13 七、南方氏雜交……………………………………………………13 八、螢光原位雜交、偵測與觀察……………………………………………………15 參、結果 一、基因組南方氏雜交分析……………………………………………………20 二、高重複性DNA序列的選殖、定序與分析……………………………………………………23 三、重複性DNA序列在種間分佈的情形……………………………………………………31 四、利用螢光原位雜交定位重複性序列Pvr-93在染色體上的位置…………………………………………………36 五、利用螢光原位雜交定位重複性序列Pvr-95在染色體上的位置…………………………………………………37 肆、討論……………………………………………………44 伍、參考文獻……………………………………………………49 | |
| dc.language.iso | zh-TW | |
| dc.title | 蝴蝶蘭兩種重複性DNA序列的分離與定性 | zh_TW |
| dc.title | Isolation and characterization of two repetitive DNA sequences in Phalaenopsis orchids | en |
| dc.date.schoolyear | 87-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 56 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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