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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.author | Shih-Pai Chang | en |
| dc.contributor.author | 張世白 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:11:31Z | - |
| dc.date.available | 2021-07-01T08:11:31Z | - |
| dc.date.issued | 1999 | |
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N (1992) Source-sink manipulation and carbohydrate metabolism in maize. Crop Sci. 32: 751-756 Saeed M and Duke SH (1990) Amylase in pea tissues with reduced chloroplast density and/or function. Plant Physiol. 94: 1813-1819 Sambrook J, Fritsch EF and Maniatis T (1989) Molecular cloning: A Laboratory Manual, Ed 2. Cold Spring Harbor Laboratory Press, New York Schwabe DL (1970) The control of leaf senescence in Kleinia articulata by photoperiod. Ann. Bot. 34: 43-55 Sharma R and Schopfer P (1982) Sequential control of phytochrome mediated synthesis de novo synthesis of β-amylase on the cotyledons of mustard (Sinapis alba L.) seedling. Planta 155: 183-189 Singh I, Nandwal AS, Kuhad MS and Singh N (1998) Biochemical changes occuring during development of leaves emerged at three different stages in pigeonpea. 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Plant Physiol. 116: 329-335 Yu SM, Kuo YH, Sheu G, Sheu YJ and Liu LF (1991) Metabolic derepression of α-amylase gene expression in suspension-cultured cells of rice. J. Biol. Chem. 266: 21131-21137 Zeeman SC, Northrop F, Smith AM and ap Rees T (1998) A starch-accumulating mutant of Arabidopsis thaliana deficient in a chloroplastic starch-hydrolysing enzyme. Plant J. 15(3): 357-365 Ziegler P and Beck E (1986) Exoamylase activity in vacuoles isolated from pea and wheat leaf protoplasts. Plant Physiol. 82: 1119-1121 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75037 | - |
| dc.description.abstract | 本論文主要探討玉米葉片之澱粉水解?在老化過程中的變化。分別以整棵植株及初生葉為材料，發現隨著葉齡的增加，會有α-澱粉水解?活性上升及β-澱粉水解?活性下降的現象。利用西方免疫檢定，發現老化過程中β-澱粉水解?活性強度和其蛋白質分子的量成正相關，顯示酵素活性下降的原因是蛋白質的破壞，而不是失活（inactivation）所致。不論是植株葉片之自然老化或葉圓盤之人為老化的試驗結果，都指出α-澱粉水解?活性的上升或β-澱粉水解?活性的下降，皆與一般用做老化指標之可溶性蛋白質含量的變化，呈現高度的相關性；顯示澱粉水解?的活性變化可以做為玉米葉片老化之良好指標，β-澱粉水解?尤其適合。北方或條帶轉印之雜合反應的結果指出，只有在發育中之未完全展開的葉片內才有β-澱粉水解?mRNA的累積，葉片一旦展開之後，其mRNA量即逐漸下降；而當此mRNA下降之時，β-澱粉水解?的活性卻仍因其蛋白質量的增加而提高。葉片切離植物體後，其β-澱粉水解?活性的下降情形不因外加植物荷爾蒙Gibberellin、Zeatin或Abscisic acid而有所改變，但α-澱粉水解?活性則會受到ABA的促進，和蔗糖的抑制；由此推論，在玉米葉片老化過程中，β-澱粉水解?活性下降與α-澱粉水解?活性上升的現象，應是透過不同的調控機制。 | zh_TW |
| dc.description.abstract | Changes of amylase activities in aging maize leaves were studied. The increase in α-amylase activity was accompanied with the decrease in β-amylase activity along the age gradient in a mature plant. Results from immunoblot analyses showed that β-amylase activity was closely correlated with the protein quantity of β-amylase during leaf senescence, and the decrease of β-amylase could be attributed to the protein degradation but not inactivation. No matter whole plant or leaf disc system was studied, leaf total soluble protein showed a highly positive correlation with β-amylase activity, and also a negative correlation with α-amylase activity during senescence. Therefore, changes in α- and β-amylase activities should be able to play a good index for leaf senescence just as total soluble protein content has been used. Based on RNA slot analysis, β-amylase mRNA was synthesized and accumulated when leaf expansion was in progress. Once the leaf reached full expansion, the β-amylase mRNA gradually decreased while the enzyme activity was still increasing owing to the protein synthesis and accumulation. In leaf disc system, the decrease of β-amylase activity during senescence could not be altered by adding phytohormones such as gibberellic acid, zeatin and abscisic acid. And α-amylase activity in leaf discs was enhanced by abscisic acid and repressed by sucrose. These results suggested that regulation of increasing α-amylase and decreasing β-amylase in senescing maize leaf might not share a common mechanism. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:31Z (GMT). No. of bitstreams: 0 Previous issue date: 1999 | en |
| dc.description.tableofcontents | 中文摘要……………………………………………………i 英文摘要……………………………………………………ii 前言……………………………………………………1 材料與方法……………………………………………………6 結果……………………………………………………16 一、成熟葉片中之β-澱粉水解?……………………………………………………16 二、初生葉中之β-澱粉水解?……………………………………………………18 三、年輕植株葉片中的β-澱粉水解?……………………………………………………19 四、葉片之人工老化與澱粉水解?的變化……………………………………………………21 五、荷爾蒙及醣類對於葉片老化過程中澱粉水解?活性的影響……………………………………………………22 討論……………………………………………………24 參考文獻……………………………………………………30 圖表……………………………………………………37 | |
| dc.language.iso | zh-TW | |
| dc.title | β-澱粉水解?在玉米葉片老化過程之表現 | zh_TW |
| dc.title | Expression of β-Amylase in Senescing Maize Leaf | en |
| dc.date.schoolyear | 87-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 36 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| Appears in Collections: | 植物科學研究所 | |
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