請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75014完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 林慧瑾 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:11:22Z | - |
| dc.date.available | 2021-07-01T08:11:22Z | - |
| dc.date.issued | 1999 | |
| dc.identifier.citation | Bergman AC, Alaiya AA, Wendler W, Binetruy B, Shoshan M, Sakaguchi K, Bergman T, Kronenwett U, Auer G, Appella E, Jornvall H, Linder (1999) Protein kinase-dependent overexpression of the nuclear protein pirin in c-JUN and RAS transformed fibroblasts. Cell Mol Life Sci 55(3):467-71 Bomsztyk K, Van Seuningen I, Suzuki H, Denisenko O, Ostrowski J (1996) Diverse molecular interactions of the hnRNP K protein. J Biol Chem 271(44):27701-6 Denisenko ON, O’Neill B, Ostrowski J, Van Seuningen I, Bomsztyk K (1996) Zik1, a transcriptional repressor that interacts with the heterogeneous nuclear ribonucleoprotein particle K protein. Mol Cell Biol 16(5):2350-60 Du Q, Melnikova IN, Gardner PD (1998) Differential effects of heterogeneous nuclear ribonucleoprotein K on Sp1- and Sp3- mediated transcriptional activation of a neuronal nicotinic acetylcholine receptor promoter. J Biol Chem 273(28):17651-9 Godbout R, Hale M, Bisgrove (1994) A human DEAD box protein with partial homology toheterogeneous nuclear ribonucleoprotein U. Gene 138(1-2):243-5 Godbout R, Packer M, Bie W(1998) Overexpression of a DEAD box protein (DDX1) inneuroblastoma and retinoblastoma cell lines. J Biol Chem 273(33):21161-8 Gondran P, Dautry F (1999) Regulation of mRNA Sp1icing and transport by the tyrosine kinase activity of src. Oncogene. 18(16):2547-55. Groysman M, Nagano M, Shaanan B, Katzav S(1998) Mutagenic analysis of Vav reveals that an intact SH3 domain is required for transformation. Oncogene 17(12):1597-606 Hobert O, Jallal B, Schlessinger J, Ullrich A (1994) Novel signaling pathway suggested by SH3 domain-mediated p95vav/heterogeneous ribonucleoprotein K interaction. J Biol Chem 269(26):17626-34 Hsieh TY, Matsumoto M, Chou HC, Schneider R, Hwang SB, Lee AS, Lai MM (1998) Hepatitis C virus core protein interacts with heterogeneous nuclear ribonucleoprotein K. J Biol Chem. 273(17):10784-91 Lee MH, Mori S, Raychaudhuri P (1995) trans-Activation by the hnRNP K protein involves an increase in RNA synthesis from the reporter genes. J Biol Chem. 270(45): 26976-85 Mamiya N, Worman HJ (1999) Hepatitis C virus core protein binds to a DEAD box RNA helicase. J Biol Chem 274(22):15751-6 Miau LH, Chang CJ, Shen BJ, Tsai WH, Lee SC (1997)Identification of heterogeneous nuclear ribonucleoprotein K(hnRNP K) as a repressor of C/EBPbeta-mediated gene activation. J Biol Chem, 273(17):10784-10791 Michelotti EF, Michelotti GA, Aronsohn AI, Levens D (1996) Heterogeneous nuclear ribonucleoprotein K is a transcription factor. J Biol Chem 271(7):3420-7 Ostareck DH, Ostareck-Lederer A, Wilm M, Thiele BJ, Mann M, Hentze MW (1997) mRNA silencing in erythroid differentiation: hnRNP K and hnRNP E1 regulate 15-lipoxygenase translation from the 3’end. FEBS Lett 403(2):113-5 Ostareck-Lederer A, Ostareck DH, Hentze MW (1998) Cytoplasmic regulatory functions of the KH-domain proteins hnRNPs K nd E1/E2. Thends Biochem Sci(11):409-11 Ostrowski J, Van Seuningen I, Seger R, Rauch CT, Sleath PR, McMullen BA, Bomsztyk K (1993) Purification, cloning, and expression of a murine phosphoprotein that binds the kappa B motif in vitro identifies it as the homolog of the human heterogeneous nuclear ribonucleoprotein K protein Description of a novel DNA-dependent phosphorylation process. J. Biol Chem 268(24):18249-58 Owsianka AM, Patel AH(1999)Hepatitis C Virus core protein interacts with a human DEAD box protein DDX3.Virology 257(2):330-40 Tomonaga T, Levens D (1994) Heterogeneous nuclear ribonucleoprotein K is a DNA-binding transactivator. J. Biol Chem 269(32):20225-8 Van Seuningen I, Ostrowski J, Bustelo XR, Sleath PR, Bomsztyk K(1995a)The K protein domain that recruits the interleukin 1-responsive K protein kinase lies adjacent to a cluster of c-Src and Vav SH3-binding sites. Implications that K protein acts as a docking platform Biochemistry 34(16):5644-50 Van Seuningen I, Ostrowski J, Bomsztyk K(1995b)Description of an IL-1-responsive kinase that phosphorylates the K protein enhancement of phosphorylation by selective DNA and RNA motifs .J Biol Chem 270(9):4875-81 Weng WK, Jarvis L, LeBien TW(1994a) Signaling through CD19 activates Vav/mitogen-activated protein kinase pathway and induces formation of a CD19/Vav/phosphatidylinositol 3-kinase complex in human Bcell precursors .J Biol Chem 269(51):32514-21 Weng Z, Thomas SM, Rickles RJ, Taylor JA, Brauer AW, Seidel-Dugan C, Michael WM, Dreyfuss G, Brugge JS (1994b)Identification of Src, Fyn, and Lyn SH3-binding proteins: implications for a function of SH3 domains .Mol Cell Biol 14(7):4509-21. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75014 | - |
| dc.description.abstract | 核糖核酸蛋白粒之K蛋白(hnRNP K)是一個具有多重功能的分子，它在基因轉錄過程中可以扮演轉錄抑制子(transcriptional repressor)的角色，以調控基因表現；又本身屬於RNP家族成員，也參與mRNA processing的過程是。並且因其可和一些信號傳遞分子結合，而被推測它可能也有傳遞信號或聯擊兩條不同信號傳遞路徑的功能。可以看出hnRNP K是一個功能複雜的分子。 hnRNP K能被某些kinase(s)進行磷酸化反應是早已被肯定的特性，它被認為是屬於serine/threonine kinase的受質，並藉由p95vav這個分子的結合，成為聯擊tyrosine kinase信號傳遞路徑的橋樑。在本篇文章中，筆者針對此特性，探討是何種kinase可以對其作用。並且確定hnRNP K本身亦可能屬於tyrosine kinase的受質。並且也是如同過去所被預測的serine/threonine kinase(Erk1)的受質。此外利用不同的kinase inhibitors處理，大致區分出它可能參與的信號傳遞路徑。同時在純化kinase的過程中，找出幾個能與hnRNP K相互作用的分子，並證實在離體的實驗裡hnRNP K確實能與這個分子(DDX1)交互作用。而hnRNP K被磷酸化後對其功能有何種改變則是進一步想要探討的方向。 | zh_TW |
| dc.description.abstract | hnRNP K is a versatile molecule which can interact with RNA, DNA, and multiple proteins, including the proto-oncogene Vav, Src-like tyrosine and inducible serine/threonine kinases, the transcription factor TBP, C/EBPβ and transcription repressor Zik1. The repressor activity of hnRNP K for C/EBPβ seems to be regulated through phosphorylation / dephosphorylation mechanisms. K protein kinase(s) was identified in whole cell extracts or nuclear extracts from K562 cells or mouse liver by GST-hnRNP K affinity chromatography and LC/MS/MS analysis. The results indicate that several kinases are involved in the phosphorylation of hnRNP K. Both tyrosine kinase(s) and Erk1 were shown to be able to phosphorylate hnRNP K. Several kinase inhibitors have been used to identify the specific K protein kinases. The involvement of these kinases in regulating hnRNP K by phosphorylation will be further studied. Multiple proteins have been detected from GST-hnRNP K affinity column chromatography. One of them, a putative RNA helicase, DDX1 has been identified. The functional implications of DDX1 and hnRNP K interaction are discussed. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:22Z (GMT). No. of bitstreams: 0 Previous issue date: 1999 | en |
| dc.description.tableofcontents | 英文摘要………………1 中文摘要………………2 第一章、 序論(Introduction)………………3 第二章、材料與方法(Materials and Methods）…6 一、質體抽取………………6 二、製備宿主細胞與細胞轉型…………8 三、質體構築………………10 四、融合蛋白的表現………………12 五、SDS聚丙烯醯胺膠體電泳……14 六、西方墨點法……………16 七、細胞培養………………19 八、全細胞液之抽取………………19 九、hnRNP K親和管柱純化激?程式……20 十、LC/MS/MS蛋白質分析………………22 十一、免疫沉澱法………………24 十二、固相激?活性測定法………………25 十三、In Gel Kinase Assay……………27 十四、In vitro translation…………30 十五、Pull-down assay………………31 第三章 結果…………48 第四章 討論……………33 參考文獻………………38 圖表………………43 | |
| dc.language.iso | zh-TW | |
| dc.title | 核糖核酸蛋白粒之K蛋白的結合磷酸激?和其他蛋白的純化與鑑定 | zh_TW |
| dc.title | Purification and identification of hnRNP K-interacting kinases and other proteins | en |
| dc.date.schoolyear | 87-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 78 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
文件中的檔案：
沒有與此文件相關的檔案。
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。