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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 林豐益 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:11:17Z | - |
| dc.date.available | 2021-07-01T08:11:17Z | - |
| dc.date.issued | 1998 | |
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Influence of seawater adaptation on prolactin and growth homone release from organ-culture pituitary of rainbow trout. Zool. Sci. 9,143-148. Yada, T., Hirano, T. and Grau, E. G. (1994). Changes in plasma levels of the two prolactins and growth hormone during adaptation to different salinities in the euryhaline tilapia, Oreochromis mossambicus. Gen. Comp. Endrocrinol. 93, 214-223. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75002 | - |
| dc.description.abstract | 水平衡（water balance）是魚類進行滲透壓調節中相當重要的一部份。藉由喝水補充流失的水分，並靠排尿去除體內多餘的水分是海水及淡水硬骨魚維持水平衡的主要機制。相較於成魚的研究，仔魚在發育過程中水平衡的研究仍相當有限。本研究以廣鹽性莫三比克吳郭魚仔魚（Oreochromis mossambicus）為研究材料，探討仔魚發育過程及面對環境鹽度變化時水份的調節。結果顯示，隨著發育，仔魚濕重及含水量逐漸增加，且適應於淡水及海水環境中並無差異。發育過程中，含水量的增加顯示仔魚對水分的需求應較成魚為高，這可能是海水仔魚喝水速率（單位元重量）較成魚高的原因之一。海水仔魚的喝水速率（單位元重量）在孵化後2-5天增加約8倍，顯示仔魚發育過程中可能伴隨著體表面積對體積比（surface area to volume ratio）及體表對水通透性（water permeability）的增加。 本研究中另一個發現是賀爾蒙cortisol可能參與仔魚喝水速率的調控。廣鹽性魚類在適應海水環境過程中，內分泌系統扮演重要的調控角色。Cortisol有助於魚類在海水環境中進行鹽類排除，因此被認為是促進海水適應的賀爾蒙。然而，cortisol是否會刺激喝水行為進而有助於水平衡，仍不清楚。本研究將吳郭魚仔魚在cortisol溶液中浸泡處理後,進行喝水速率及含水量的測定,以瞭望cortisol對水平衡的影響。結果顯示，孵化4天的海水適應仔魚在10mg/l cortisol中浸泡4小時後喝水速率即受到抑制，並在14小時後導致含水量的下降。淡水適應仔魚喝水速率也受cortisol的抑制，在處理8小時後，喝水速率降為控制組的50%，但並未導致含水量的下降。然而，當淡水仔魚經cortisol處理後再急遽轉移到20?海水中，被抑制的喝水速率會快速增加，且增加速率比未經cortisol處理的控制組快。顯示cortisol在吳郭魚仔魚由淡水適應到海水的過程中，確實有促進仔魚喝水的作用；然而在未面臨鹽度變化時，給予仔魚外源性cortisol反而會抑制仔魚喝水。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:17Z (GMT). No. of bitstreams: 0 Previous issue date: 1998 | en |
| dc.description.tableofcontents | 摘要…………………………………………………………………………………………………1 前言…………………………………………………………………………………………………3 硬骨魚類滲透壓調節…………………………………………………………………………3 硬骨魚類胚胎及仔魚時期之水平衡…………………………………………………………3 賀爾蒙在魚類滲透壓調節所扮演之角色……………………………………………………5 Proactin、GH、IGF-Ⅰ…………………………………………………………………5 AngiotensnⅡ………………………………………………………………………………7 Cortisol……………………………………………………………………………………8 賀爾蒙在仔魚滲透壓調節之研究 9 研究動機……………………………………………………………………………………11 材料與法……………………………………………………………………………………12 一、實驗動物………………………………………………………………………………12 實驗魚種來源及飼育條件…………………………………………………………………12 受精卵之取得與仔魚馴養…………………………………………………………………12 二、實驗方法………………………………………………………………………………13 喝水速率之測量……………………………………………………………………………13 決定適當浸泡時間…………………………………………………………………………14 決定體表碳14C-dextran 沾附之影響……………………………………………………15 仔魚濕重、乾重及含水量之測量…………………………………………………………15 仔魚體長及卵黃囊直徑之測量……………………………………………………………15 Cortisol含量之測定………………………………………………………………………16 三、實驗設計……………………………………………………………………………………18 實驗一：海水適應及淡水適應仔魚發育過程中水平衡變化……………………………………………………18 實驗二：急鹽度轉移下仔魚之水平衡………………………………………………………………………18 淡水轉移實驗……………………………………………………………………………………18 海水轉移實驗……………………………………………………………………………………19 實驗三：海水適應仔魚以cortisol浸泡24小時後水平衡之變化……………………………………19 實驗四：海水及淡水適應仔魚以cortisol浸泡不同時問後水平衡之變化…………………………………l9 實驗五：以cortisol浸泡後再進行海水轉移……………………………………………………20 結果……………………………………………………………………………………………………………………21 預備實驗……………………………………………………………………………………21 決定適當浸泡時問……………………………………………………………………………………21 決定體表碳14C-dextran 沾附之影響………………………………………………………………………………21 實驗一：比較海水適應及淡水適應仔魚之水平衡……………………………………………………………………22 實驗二：急遽鹽度轉移下仔魚之水平衡………………………………………………………………………………22 淡水轉移實驗……………………………………………………………………………………22 海水轉移實驗……………………………………………………………………………………23 實驗三：海水適應仔魚以cortisol浸泡24小時後水平衡之變化………………………………………………23 實驗四：海水及淡水適應仔魚以cortisol浸泡不同時間後水平衡之變化………………………………………24 實驗五：以cortisol浸泡後再進行海水轉移………………………………………………………………………24 討論……………………………………………………………………………………26 喝水速率之測定……………………………………………………………………………………26 仔魚發育中水平衡之變化……………………………………………………………………………………27 Cortisol對仔魚喝水速率之影響……………………………………………………………………………………30 Cortisol影響仔魚喝水之可能機制……………………………………………………………………………………32 Cortisol 對仔魚發育之影響……………………………………………………………………………………33 結語與展望……………………………………………………………………………………34 謝辭……………………………………………………………………………………35 參考文獻……………………………………………………………………………………36 圖與表……………………………………………………………………………………40 | |
| dc.language.iso | zh-TW | |
| dc.title | 皮質醇對吳郭魚仔魚水平衡之影響 | zh_TW |
| dc.title | Effects of Cortisol on Water Balance in Tilapia Larvae (Oreochromis mossambicus) | en |
| dc.date.schoolyear | 86-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 68 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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