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標題: | 以電控微流體平台建構微型生物合成致動器 Constructing a Micro-Biohybrid Actuator with an Electromicrofluidic Platform |
作者: | Chu-Chi Lin 林鉅其 |
指導教授: | 范士岡 |
關鍵字: | 電控微流體,C2C12,細胞排列,近場通訊,生物合成致動器, Electro-microfluidic,C2C12,cell alignments,NFC,bio-hybrid actuator, |
出版年 : | 2019 |
學位: | 碩士 |
摘要: | 近年來微小的生物合成致動器被廣泛的研究,不論是細胞合成致動器或者生物相容之材料合成致動器,都朝著遠端驅控的技術前進,以增加其在醫療應用上及其他領域中應用的可能性,其中電場與磁場為最常見的方式。本研究嘗試利用電控微流體平臺跨尺度同時操控多種水膠與細胞的優勢,將生物細胞如小鼠骨骼肌纖維母細胞 (Skeletal muscle myoblasts of Mus musculus, C2C12) 混入滲透壓適合其生長的之水膠溶液Gelatin methacryloyl (GelMA) 後,先以良性絕緣水膠材料Poly(ethylene glycol) diacrylate (PEGDA, Mn: 575) 以電極圖形定義出的中空結構,進行塑型與固化後,再將含有C2C12的GelMA水膠以介電濕潤原理 (Electrowetting On Dielectric, EWOD) 驅動並建構出三維定量體積之微型生物合成致動器,此結構設計除了可提供肌肉組織兩端自由取下或套上固定點的能力外,也可利用PEGDA 的電性性質,結合事先以多次光刻製程準備的多層聚醯亞胺 (Polyimide, PI)軟性基材與符合特定通訊頻段 (Near Field Communication, NFC) 的感應天線,將該裝置上裸露於外頭的電子零件以之封裝,並觀測其受特定射頻 (Radio frequency, RF) 13.56 MHz響應,所產生之電流對誘導致動器進行可遠端操控之運動模式。肌母細胞在分化時會彼此融合並形成多核的肌小管,根據有效文獻顯示,施加外部應力以抵抗C2C12在生長時的收縮力,能提升細胞在結構中可控的排列性,故本實驗設計了兩個固定端於PDMS載台之上,以固定的方式給予細胞一軸向作用力。目前已經能夠培養出具有排列性的生物合成致動器,並且利用適當的電訊號刺激該肌肉組織,達到可預期的致動行為。 The bio-hybrid actuators have been widely developed in the recent years. No matter it’s made by cells or biocompatible materials, remote control seems to be a collective target that most groups want to develop. In order to increase the possibility of the medical applications or in other fields. Electric field and magnetic field are the most common techniques so far. Here we utilize the superiority of the Electro-microfluidic (EMF) platform which can manipulate multiple hydrogels and cells simultaneously. First, with an excellent insulating property of PEGDA, we made it into a hollow structure by EMF platform. After corss-linking, the GelMA hydrogel mixed with C2C12 will be manipulated by EWOD to form the shape of a muscle tisse. This designing can help the C2C12 muscle tissue be freestanding with a previously prepared PDMS fixing mold. Furthermore, the electrical properties of PEGDA can be an impeccable virtue to combine and also encapsulate the electronics on the flexible printed circuit devices which are able to provide energy without contact. With a particular radio frequency at 13.56 MHz passed through a well designed antenna, it will operate an induced current response to achieve the remote control. When myoblasts conduct a differentiation, they will transform into multi-nuclei myotubes. According to literatures, when muscle tissue applied directional stress oppositely during the growing process, can increase the controllable arrangement of C2C12. With the fixing anchors on the PDMS mold, we have already constructed an C2C12 muscle tissue and caused controllable actuations by the electrical stimulation. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74973 |
DOI: | 10.6342/NTU201904098 |
全文授權: | 有償授權 |
顯示於系所單位: | 機械工程學系 |
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