請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74809
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鍾嘉綾(Chia-Lin Chung) | |
dc.contributor.author | Chieh-Yi Chen | en |
dc.contributor.author | 陳杰宜 | zh_TW |
dc.date.accessioned | 2021-06-17T09:07:59Z | - |
dc.date.available | 2024-12-02 | |
dc.date.copyright | 2019-12-02 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-11-25 | |
dc.identifier.citation | Amatulli, M. T., Spadaro, D., Gullino, M. L., and Garibaldi, A. 2010. Molecular identification of Fusarium spp. associated with bakanae disease of rice in Italy and assessment of their pathogenicity. Plant Pathol. 59:839-844.
Amoah, B. K., Rezanoor, H. N., Nicholson, P., and Mac-Donald, M. V. 1995. Variation in the Fusarium section Liseola: pathogenicity and genetic studies of Fusarium moniliforme Sheldon from different hosts in Ghana. Plant Pathol. 44:563-572. Anderson, L. L., and Webster, R. K. 2005. A comparison of assays for Gibberella fujikuroi and their ability to predict resulting bakanae from seed sources in California. Phytopathology 95 S4:6. Bacon, C. W., Porter, J. K., Norred W. P., and Leslie, J. F. 1996. Production of fusaric acid by Fusarium species. Appl. Environ. Microbiol. 62:4039-4043. Bashyal, B. M., Aggarwal, R., Gupta, S., and Banerjee, S. 2012. Ecology and genetic diversity of Fusarium spp. associated with bakanae disease of rice. Pages 577 in: Modern Trends in Microbial Biodiversity of Natural Ecosystem. A. Sinha, B. K. Sharma and M. Srivastava, eds., Biotech Books Publisher, New Delhi. Bashyal, B. M., Aggarwal, R., Sharma, S., Gupta, S., Rawat, K., Singh, D., and Krishnan, S. G. 2016a. Occurrence, identification and pathogenicity of Fusarium species associated with bakanae disease of basmati rice in India. Eur. J. Plant. Pathol. 144:457-466. Bashyal, B. M. 2018. Etiology of an emerging disease: bakanae of rice. Indian Phytopathol. 71:485-494. Capote, N., Pastrana, A. M., Aguado, A., and Sanchez-Torres, P. 2012. Molecular tools for detection of plant pathogenic fungi and fungicide resistance. Plant pathol. 151-202. Cappellini, R. A., and Peterson, J. L. 1965. Macroconidium formation in submerged cultures by a nonsporulating strain of Gibberella zeae. Mycologia 57:962-966. Carneiro, G. A., Matić, S., Ortu, G., Garibaldi, A., Spadaro, D., and Gullino, M. L. 2017. Development and validation of a TaqMan real-time PCR assay for the specific detection and quantification of Fusarium fujikuroi in rice plants and seeds. Phytopathology 107:885-892. Cerda-Olmedo, E., Fernandez-Martin, R., and Avalos, J. 1994. Genetics and gibberellin production in Gibberella fujikuroi. Antonie Van Leeuwenhoek 65:217-225. Chen, S. Y., Lai, M. H., Tung, C. W., Wu, D. H., Chang, F. Y., Lin, T. C., and Chung, C. L. 2019. Genome-wide association mapping of gene loci affecting disease resistance in the rice-Fusarium fujikuroi pathosystem. Rice RICE-D-19-00129R1. Chen, Y. C., Lai, M. H., Wu, C. Y., Lin, T. C., Cheng, A. H., Yang, C. C., Wu, H. Y., Chu, S. C., Kuo, C. C., Wu, Y. F., Lin, G. C., Tseng, M. N., Tsai, Y. C., Lin, C. C., Chen, C. Y., Huang, J. W., Lin, H. A., and Chung, C. L. 2016. The genetic structure, virulence, and fungicide sensitivity of Fusarium fujikuroi in Taiwan. Phytopathology 106: 624-635. Cheng, A. P. 2018. Exploring the transcriptome of rice seedlings affected by bakanae disease. MSc. Thesis. National Taiwan University. Cook, M. E., Graham, L. E., Botha, C. E. J., and Lavin, C. A. 1997. Comparative ultrastructure of plasmodesmata of Chara and selected Bryophytes: Toward an elucidation of the evolutionary origin of plant plasmodesmata. Am. J. Bot. 84:1169-1178. Datnoff, L. E. 1994. Influence of mineral nutrition of rice on disease development. Pages 89-100 in: Rice Pest Science and Management. P. S. Teng, K. L. Heong, and K. Moody, eds. International Rice Research Institute, Los Baños, Phillipines. Desjardins, A. E., Manandhar, H. K., Plattner, R. D., Manandhar, G. G., Poling, S. M., and Maragos, C. M. 2000. Fusarium species from Nepalese rice and production of mycotoxins and gibberellic acid by selected species. Appl. Environ. Microbiol. 66:1020-1025. Diaz-Sanchez, V., Avalos, J., and Limon, M. C. 2012. Identification and regulation of fusA, the polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi. Appl. Environ. Microbiol. 78:7258-7266. Doyle, J. J., and Doyle, J. L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19:11-15. Eizenga, G. C., Ali, M., Bryant, R. J., Yeater, K. M., McClung, A. M., and McCouch, S. R. 2014. Registration of the rice diversity panel 1 for genomewide association studies. J. Plant Regist. 8:109-116. FAO. 2019. Food and Agriculture Organization of the United Nations. FAOSTAT Database FAO, Rome. Geiser, D. M., Jimenez-Gasco, M. M., Kang, S., Makalowska, I., Veeraraghavan, N., Ward, T. J., Zhang, N., Kuldau, G. A., and O’Donnell, K. 2004. FUSARIUM-ID v. 1.0: a DNA sequence database for identifying Fusarium. Eur. J. Plant. Pathol. 110:473-479. Gupta, A. K., Singh, Y., Jain, A. K., and Singh, D. 2014. Prevalence and incidence of bakanae disease of rice in northern India. J. AgriSearch 1:233-237. Gupta, A. K., Solanki, I. S., Bashyal, B. M., Singh, Y., and Srivastava, K. 2015. Bakanae of rice-an emerging disease in Asia. J. Anim. Plant Sci. 25:1499-1514. Haq, M., Mia, M. A. T., Rabbi, M. F., and Ali, M. A. 2011. Incidence and severity of rice diseases and insect pests in relation to climate change. Pages 445-457 in: Climate Change and Food Security in South Asia. R. Lal, M. V. K. Sivakumar, S. M. A. Faiz, A. H. M. M. Rahman and K. R. Islam, eds., Springer, Dordrecht. Hori, S. 1898. Some observations on bakanae disease of the rice plant. Mem. Agric. Res. Sta. (Tokyo) 12:110-119. Hossain, M. T., Khan, A., Chung, E. J., Rashid, M. H. O., and Chung, Y. R. 2016. Biological control of rice bakanae by an endophytic Bacillus oryzicola YC7007. Plant Pathol. J. 32:228. Hou, Z., Xue, C., Peng, Y., Katan, T., Kistler, H. C., and Xu, J. R. 2002. A mitogen-activated protein kinase gene (MGV1) in Fusarium graminearum is required for female fertility, heterokaryon formation, and plant infection. Mol. Plant Microbe. In. 15:1119-1127. Hsieh, W. H., Smith, S. N., and Snyder, W. C. 1977. Mating groups in Fusarium moniliforme. Phytopathology 67:1041-1043. Hsu, C. C., Huang, J. W., and Chen, C. Y. 2013. The cause of rice bakanae disease in Taiwan. Pl. Path. Bull. 22:279-289. Huang, T. C., and Chu, S. C. 2009. The occurrence and control of rice bakanae disease in Taiwan. Proceeding of Symposium on Achievement and Perspectives of Rice Protection in Taiwan:29-43. Chiayi Agricultural Experiment Station, Taiwan Agricultural Research Institute, Chiayi. Hwang, I. S., Kang, W. R., Hwang, D. J., Bae, S. C., Yun, S. H., and Ahn, I. P. 2013. Evaluation of bakanae disease progression caused by Fusarium fujikuroi in Oryza sativa L. J. Microbiol. 51:858-865. Hwang, I. S., and Ahn, I. P. 2016. Multi-Homologous recombination-based gene manipulation in the rice pathogen Fusarium fujikuroi. Plant Pathol. J. 32:173-181. Ito, S. and Kimura, J. 1931. Studies on Bakanae disease of the rice plant. Hokkaido Agri. Exp. Stn. 27:1-95. Jeon, Y. A., Yu, S. H., Lee, Y. Y., Park, H. J., Lee, S., Sung, J. S., Kim, Y. G., and Lee, H. S. 2013. Incidence, molecular characteristics and pathogenicity of Gibberella fujikuroi species complex associated with rice seeds from Asian countries. Mycobiology 41:225-233. Kanjanasoon, P. 1965. Studies on bakanae disease of rice in Thailand. Doc. Thesis. Tokyo University. Kankanala, P., Czymmek, K., and Valent, B. 2007. Roles for rice membrane dynamics and plasmodesmata during biotrophic invasion by the blast fungus. The Plant Cell 19:706-724. Karov, I. K., Mitrev, S. K., and Kostadinovska, E. D. 2009. Gibberella fujikuroi (Sawada) Wollenweber, the new parasitical fungus on rice in the Republic of Macedonia. Proc. Nat. Sci. Matica Srpska Novi Sad. 116:175-182. Kato, A., Miyake, T., Nishigata, K., Tateishi, H., Teraoka, T., and Arie, T. 2012. Use of fluorescent proteins to visualize interactions between the Bakanae disease pathogen Gibberella fujikuroi and the biocontrol agent Talaromyces sp. KNB-422. J. Gen. Plant Pathol. 78:54-61. Kaur, J., Pannu, P. P. S., and Sharma, S. 2014. Morphological, biochemical and molecular characterization of Gibberella fujikuroi isolates causing bakanae disease of basmati rice. J. Mycol. Pl. Pathol. 44:78-82. Khan, J. A., Jamil, F. F., and Gill, M. A. 2000. Screening of rice varieties/lines against bakanae and bacterial leaf blight (BLB). Pakistan J. Phytopath. 12:6-11. Kim, B. R., Han, K. S., Hahm, S. S., Kwon, M. K., and Nam, Y. G. 2015. Occurrence of the rice bakanae disease in Chungnam province. Res. Plant Dis. 21:154. Kuo, C. C., Liao, C. T., Huang, D. C., Chen, Y. C., and Chung, C. L. 2014. Investigation of rice bakanae disease in central Taiwan: occurrence, pathogen identification, and fungicide resistance assay. Research Bulletin of Taichung District Agricultural Research and Extension Station 125:11-28. Kurosawa, E. 1926. Experimental studies on the nature of the substance secreted by the bakanae fungus. Nat. Hist. Soc. Formosa 16:213-227. Kurosawa, E. 1928. On the symptoms and causal fungus of the' Bakanac' disease of rice plants. Trans. Nat. Hist. Soc. Formosa 18:230. Kvas, M., Marasas, W. F. O., Wingfield, B. D., Wingfield, M. J., and Steenkamp, E. T. 2009. Diversity and evolution of Fusarium species in the Gibberella fujikuroi complex. Fungal Divers. 34:1-21. Lee, G. W. 2016. Studies on the association of Fusarium fujikuroi and Aspergillus flavus with rice seeds. MSc. Thesis. National Chung Hsing University. Leslie, J. F. and Summerell, B. A. 2006. The Fusarium Laboratory Manual. Blackwell Professional publishing, Ames, Iowa, USA. Limon, M. C., Rodríguez-Ortiz, R., and Avalos, J. 2010. Bikaverin production and applications. Appl. Microbiol. Biotechnol. 87:21-29. Lin, C. Y. 2015. Characterization of Fusarium fujikuroi, with reference to the survival and the course of disease occurrence. MSc. Thesis. National Chung Hsing University. Ma, L., Ji, Z., Bao, J., Zhu, X., Li, X., Zhuang, J., Yang, C., and Xia, Y. 2008. Responses of rice genotypes carrying different dwarf genes to Fusarium moniliforme and gibberellic acid. Pl. Prod. Sci. 11: 134-138. Mai, W. F. and Abawi, G. S. 1987. Interactions among root-knot nematodes and Fusarium wilt fungi on host plants. Annu. Rev. Phytopathol. 25:317-338. Manandhar, J. 1999. Fusarium moniliforme in rice seeds: its infection, isolation and longevity. ZPflkrankh Pflschutz 106:598–607. Marasas, W. F. O., Thiel, P. G., Rabie, C. J., Nelson, P. E., and Toussoun, T. A. 1986. Moniliformin production in Fusarium section Liseola. Mycologia 78:242-247. Matic, S., Spadaro, D., Prelle, A., Gullino, M. L., and Garibaldi, A. 2013. Light affects fumonisin production in strains of Fusarium fujikuroi, Fusarium proliferatum, and Fusarium verticillioides isolated from rice. Int. J. Food Microbiol. 166:515-523. Matic, S., Gullino, M. L., and Spadaro, D. 2017. The puzzle of bakanae disease through interactions between Fusarium fujikuroi and rice. Front. Biosci. (Elite Ed) 9:333-344. Michielse, C. B., van Wijk, R., Reijnen, L., Cornelissen, B. J., and Rep, M. 2009. Insight into the molecular requirements for pathogenicity of Fusarium oxysporum f. sp. lycopersici through large-scale insertional mutagenesis. Gen. Biol. 10:R4. Moretti, A., Logrieco, A., Bottalico, A., Ritieni, A., Fogliano, V., and Randazzo, G. 1996. Diversity in beauvericin and fusaproliferin production by different populations of Gibberella fujikuroi (Fusarium section Liseola). Sydowia 48:44-56. Naito, H., Saito, K., Furuya, H., and Fuji, S. 2008. Morphology and fungal growth in elongated rice seedlings infected with Gibberella fujikuroi, and control of the disease in a flooded nursery. Jpn. J. Phytopathol. 74:321-327. Nakamura, T., Mitsuoka, K., Sugano, M., Tomita, K., and Murayama, T. 1985. Effects of auxin and gibberellin on conidial germination and elongation of young hyphae in Gibberella fujikuroi and Penicillium notatum. Plant Cell Physiol. 26:1433-1438. Nancy, A. E. 2002. Foolish seedlings and DELLA regulators: the functions of rice SLR1 and Arabidopsis RGL1 in GA signal transduction. Plant Cell 14:1-6. Niehaus, E. M., Munsterkotter, M., Proctor, R. H., Brown, D. W., Sharon, A., Idan, Y., Young, L. O., Sieber, C. M., Novak, O., Pencik, A., Tarkowska, D., Hromadova, K., Freeman, S., Maymon, M., Elazar, M., Youssef, S. A., El-Shabrawy, E. S. M., Shalaby, A. B. A., Houterman, P., Brock, N. L., Burkhardt, L., Tsavkelova, E. A., Dickschat, J. S., Galuszka, P., Guldener, U., and Tudzynski, B. 2016. Comparative “omics” of the Fusarium fujikuroi species complex highlights differences in genetic potential and metabolite synthesis. Genome Biol. Evol. 8:3574-3599. Nirenberg, H. 1976. Untersuchungen uber die morphologische und biologische differenzierung in Fusarium Sektion Liseola. Mitt. Biol. Bundesansi. Land-Forstwirtsch. Berlin-Dahlem 169:1-117. Nisikado, Y., and Kimura, K. 1939. Microscopic examination of the rice stem infected by Fusarium fujikuroi (First report). Berichte des Ohara Instituts für landwirtschaftliche Forschungen. 31:341-347. (In Japanese) Nisikado, Y., and Kimura, K. 1941. A contribution to the pathological anatomy of rice plants affected by Gibberella fujikuroi (Saw.). Ber. Ohara Inst. landw. Forsch. 8:421-426. O'Donnell, K., Cigelnik, E., and Nirenberg, H. I. 1998. Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 90:465-493. Ou, S. H. 1985. Bakanae disease and foot rot. Pages 262-272 in: Rice diseases. Commonwealth Mycological Institute, Kew, Surrey, England, UK. Ou, S. H. 1987. Rice Diseases. CAB International, Commonwealth Mycological Institute, Kew, Surrey, England, UK. Papademetriou, M. K., 2000. Rice Production in the Asia-Pacific region: issues and perspectives. Pages 4-25 in: Bridging the rice yield gap in the Asia-Pacific Region. M. K. Papademetriou, J. D. Frank , and M. H. Edward , eds. FAO Regional Office for Asia and the Pacific, Bangkok, Thailand. Roth, R., Chiapello, M., Montero, H., Gehrig, P., Grossmann, J., O’Holleran, K., Hartken, D., Walters, F., Yang, S. Y., Hillmer, S., Schumacher, K., Bowden, S., Craze, M., Wallington, E. J., Miyao, A., Sawers, R., Martinoia, E., and Paszkowski, U. 2018. A rice serine/threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane. Nat. Commun. 9:4677. Saremi, H., and Farrokhi, F. 2004. Study on bakanae disease of rice and evaluation of cultivars in Gilan and Zanjan provinces. Pages 358-364 in: Iran Proceedings Fourth International Iran and Russia conference. Singh, R., and Sunder, S. 1997. Foot rot and bakanae of rice: retrospects and prospects. Intern. J. Trop. Pl. Dis. 15:153-176. Sharma, V. K. and Bagga, P. S. 2007. Pathogenic behaviour of Fusarium moniliforme isolates causing foot rot disease in basmati rice. Pl. Dis. Res. 22:165-166. Siciliano, I., Carneiro, G. A., Spadaro, D., Garibaldi, A., and Gullino, M. L. 2015. Jasmonic acid, abscisic acid, and salicylic acid are involved in the phytoalexin responses of rice to Fusarium fujikuroi, a high gibberellin producer pathogen. J. Agric. Food Chem. 63:8134-8142. Sidharthan, V. K., Aggarwal, R., and Shanmugam, V. 2019. Fusarium wilt of crop plants: Disease development and management. Pages 519-533 in: Wilt Diseases of Crops. A. Bhattacharyya, B. N. Chakraborty, R. N. Pandey, D. Singh and S. C. Dubey, eds. Today and Tomorrow Printers and Publisher, New Delhi, India. Sperotto, R. A., Vasconcelos, M. W., Grusak, M. A., and Fett, J. P. 2017. Whole-plant mineral partitioning during the reproductive development of rice (Oryza sativa L.). Span. J. Agric. Res. 15:e0802-11. Steffens, B., Wang, J., and Sauter, M. 2006. Interactions between ethylene, gibberellin and abscisic acid regulate emergence and growth rate of adventitious roots in deepwater rice. Planta 223:604-612. Sun, S. K. 1975. The diseases cycle of rice bakanae disease in Taiwan, Proc. Natl. Sci. Counc. Repub. China 8:245-256. Sun, S. K. and Snyder, W. C. 1981. The bakanae disease of the rice plant. Pages 104-113 in: Fusarium: Diseases, Biology and Taxonomy. P. E. Nelson, T. A. Toussoun, and R. J. Cook, eds. The Pennsylvania State University Press, University Park, PA, USA. Sunani, S. K., Bashyal, B. M., Aggarwal, R., Prakash, G. 2017. Conidial germination of Fusarium fujikuroi causing bakanae disease of rice. Environ. Ecol. 35:2790-2794. Sunani, S. K. 2016. Understanding the seed borne infection of Fusarium fujikuroi inciting bakanae disease of rice. MSc. Thesis. ICAR-Indian Agricultural Research Institute. Sunder, S. and Satyavir. 1998a. Survival of Fusarium moniliforme in soil, grains and stubbles of paddy. Ind. Phytopath. 51:47-50. Sunder, S. and S. Satyavir. 1998b. Vegetative compatibility, biosynthesis of GA3 and virulence of Fusarium moniliforme isolates from bakanae disease of rice. Pl. Pathol. 47:767-772. Surek, H., and Gumustekin, H. 1994. Research activities on controlling rice bakanae and foot rot disease (Fusarium moniliforme) in Turkey. FAO MedNet Rice: Breeding and Biotechnology Groups: Proc. workshops Montpellier: CIHEAM., Cahiers Options Mediterraneennes 8:27- 30. Thomas, K. M. 1931. A new paddy disease in Madras. Madras Agril. J. 19:34-36. Watanabe, S., Kumakura, K., Izawa, N., Nagayama, K., Mitachi, T., Kanamori, M., Teraoka, T., and Arie, T. 2007. Mode of action of Trichoderma asperellum SKT-1, a biocontrol agent against Gibberella fujikuroi. J. Pestic. Sci. 32: 222-228. Watanabe, Y. 1974. The possibility of soil transmission in bakanae disease and the contamination of seed with causal fungus during the hastening process of seed germination. Bull-Tokai-KinkiNat. Agric. Exp. St. 27:35-41. Webster, R. K., and Gunnell, P. S. 1992. Compendium of rice disease. First edition, The American Phytopathological Society Press, St. Paul, Minnesota, USA. Wiemann, P., Sieber, C. M. K., von Bargen, K. W., Studt, L., Niehaus, E. M., Espino, J. J., Huß, K., Michielse, C. B., Albermann, S., Wagner, D., Bergner, S. V., Connolly, L. R., Fischer, A., Reuter, G., Kleigrewe, K., Bald, T., Wingfield, B. D., Ophir, R., Freeman, S., Hippler, M., Smith, K. M., Brown, D. W., Proctor, R. H., M€unsterkötter, M., Freitag, M., Humpf, H. U., G€uldener, U., and Tudzynski, B. 2013. Deciphering the cryptic genome: genome-wide analyses of the rice pathogen Fusarium fujikuroi reveal complex regulation of secondary metabolism and novel metabolites. PLoS Pathog. 9:e1003475. Yabuta, T. and Sumiki, Y. 1938. On the crystal of gibberellin, a substance to promote plant growth. J. Agric. Chem. Soc. Japan 14:1526. Yoshida, S., Ohnishi, Y., and Kitagishi, K. 1959. Role of siucon in rice nutrition. Soil Sci. Plant Nutr. 5:127-133. Zainudin, N. A. I. M., Razak, A. A., and Salleh, B. 2008a. Bakanae disease of rice in Malaysia and Indonesia: etiology of the causal agent based on morphological, physiological and pathogenicity characteristics. J. Plant Protect Res. 48:475-485. Zainudin, N. A. I. M., Razak, A. A., and Salleh, B. 2008b. Secondary metabolite profiles and mating populations of Fusarium species in section Liseola associated with bakanae disease of rice. Malays. J. Microbiol. 4:6-13. Zainudin, N. A. I. M., and Salleh, B. 2010. Variability of Fusarium species associated with bakanae disease of rice based on virulence, vegetative and biological compatibility. Sydowia 62:89-104. Zheng, Z. W., Chang, S. J., and Chu, S. C. 2016. The occurrence of Bankanae disease and fungicide resistance of Fusarium fujikuroi on rice paddy, seedlings and seeds from Miaoli. Research Bulletin of Miaoli District Agricultural Research and Extension Station 4:59-72. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74809 | - |
dc.description.abstract | 水稻徒長病由 Fusarium fujikuroi Nirenberg所造成,其廣泛發生於全球水稻種植地區。本病於幼苗期引起葉片褪綠、植株纖細徒長等病徵,造成成株節上長出不定根,並使稻穗不稔,因而導致 30-95% 的減產。由於目前對於 F. fujikuroi 在水稻上的致病過程所知有限,本研究目的為深入瞭解徒長病菌在水稻不同生育期的侵染與拓殖情形。本研究使用人工接種之抗病 (臺農67號,TNG67) 與感病 (Zerawchanica karatals, ZK) 品種幼苗,以及收集自田間之自然感染成株 [高雄139 號 (KS139), 台梗2號 (TK2) 及臺南11號 (TN11)],透過徒手切片配合顯微觀察,和即時定量PCR (real-time quantitative PCR, qPCR) 技術進行菌量測定。人工接種試驗使用帶有綠色螢光蛋白 (green fluorescent protein, GFP) 基因之 F. fujikuroi 轉殖菌株,其為本研究以原生質體 - polyethylene glycol (PEG) 轉殖法所建立。利用螢光顯微鏡進行觀察,發現徒長病菌於侵染初期,由胚逐漸侵入根基部及莖基部,以菌絲直接穿透莖和根的表皮層進行感染,菌絲在細胞間及細胞內延伸。人工接種之幼苗以每 3 公分為一段,以 qPCR 測定其中的菌量,發現徒長病菌集中在胚、根基部與莖基部;接種 14 天後,在胚中之菌量遠較根及莖基部多。TNG67 及 ZK 於根及莖組織所帶菌量皆相似,顯示初期抗、感病品種在巨觀病徵上的差異,與實際侵染之菌量無直接關聯。以 0.5 mg/L 之 gibberellic acid-3 (GA3) 處理發芽水稻種子後,ZK 較 TNG67 幼苗有更明顯之徒長現象,表示 ZK 對徒長病的高感病性可能與其對 GA3 之高感受性相關。於分蘗及孕穗期之罹病植株中,病原菌在高雄139號及台梗2號品種幾乎僅嚴重纏據維管束,而在臺南11號則亦觀察到少量纏據維管束、並擴展至鄰近薄壁細胞之情形。此外病原菌主要分布於 50 公分以下之莖桿 (~60%植株高度),葉片中則並未測得病原菌。徒長病菌對於稻株的莖節或節間組織並無偏好性,而莖節上長出不定根之病徵,不代表該莖節已被病原菌所侵染,不定根的出現亦無法反映纏據於植株中之病原菌量。期望透過本研究,為 F. fujikuroi 與水稻之交互作用提供新資訊。 | zh_TW |
dc.description.abstract | Bakanae disease of rice is caused by Fusarium fujikuroi Nirenberg. The disease occurs worldwide in rice-growing areas. The infected seedlings are thinner, taller, and paler than healthy ones. The infected adult plants show adventitious roots on the node or empty panicles, leading to yield losses ranging from 30-95%. Due to the lack of knowledge on the pathogenesis of bakanae disease of rice plant caused by F. fujikuroi, this study aims to explore the infection and colonization patterns of F. fujikuroi during different rice growth stages. Artificially inoculated rice seedlings [a resistant cultivar Tainung 67 (TNG67) and a susceptible cultivar Zerawchanica karatals (ZK)] and naturally infected adult plants [Kaohsiung 139 (KH139), Taikeng 2 (TK2), and Tainan 11 (TN11)] were investigated by hand sectioning and microscopic observation as well as real-time quantitative PCR (qPCR). A green fluorescent protein (GFP)-tagged F. fujikuroi transformant generated by protoplast-polyethylene glycol (PEG) transformation method was used for artificial inoculation. By fluorescence microscopy, we observed direct penetration of F. fujikuroi hyphae through the epidermis of stem and roots. F. fujikuroi infected intra- and intercellularly from the embryo to the basal part of rice stems and roots during early infection stage. qPCR analysis of consecutive 3-cm segments of the whole seedlings revealed that F. fujikuroi was mainly detected in the embryo, basal stem, and basal roots. Since 14 days post inoculation, the biomass of F. fujikuroi in the embryo became much higher than in the stem and roots. TNG67 and ZK contained similar amounts of F. fujikuroi in the stem and root tissues, implying that the macroscopic bakanae phenotypes were not necessarily correlated with the pathogen quantity. Treatment of germinated seeds with 0.5 mg/L of gibberellic acid-3 (GA3) resulted in significantly more elongated seedlings of ZK than TNG67, suggesting that the bakanae susceptibility in ZK is likely associated with its higher sensitivity to GA3. In the infected plants at the tillering and booting stage, the hyphae were found heavily colonizing almost entirely in vascular bundles in cultivars KH139 and TK2 but in TN11 hyphae colonizing less abundantly in vascular bundles as well as the surrounding parenchyma cells. Moreover, F. fujikuroi was mainly detected in the stem below 50 cm (~60% of plant height), but undetectable in the leaves. F. fujikuroi showed no preference for nodes or internodes, and the emergence of adventitious roots from a certain node did not indicate node invasion of F. fujikuroi. In addition, the appearance of adventitious roots did not reflect abundant colonization of F. fujikuroi in the plant. We hope this study can provide new insights into the interactions between F. fujikuroi and rice. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T09:07:59Z (GMT). No. of bitstreams: 1 ntu-108-R06633005-1.pdf: 4267035 bytes, checksum: b898c945242151eae58f60980b31b69f (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 口試委員會審定書 I
中文摘要 II Abstract IV CONTENTS VI LIST OF TABLE IX LIST OF FIGURES X Chapter 1 Introduction 1 1.1 Rice and Bakanae disease 1 1.2 Symptoms and secondary metabolites 4 1.3 Bakanae epidemiology 5 1.4 Pathogenesis of F. fujikuroi 7 1.5 Quantification of F. fujikuroi in rice plants 8 Chapter 2 Materials and Methods 11 2.1 Plant materials 11 2.2 Inoculation of F. fujikuroi 11 2.3 Construction of fluorescent F. fujikuroi isolates 12 2.3.1 Vector construction 12 2.3.2 Preparation of F. fujikuroi protoplasts 14 2.3.3 Transformation of F. fujikuroi 15 2.3.4 Confirmation of fluorescent transformants 16 2.4 DNA extraction 16 2.5 Quantification of F. fujikuroi by qPCR 17 2.6 Macroscopic observation 19 2.7 Hand sectioning and microscopic examination 19 2.8 Gibberellic acid treatment 20 Chapter 3 Results 21 3.1 Construction of fluorescent F. fujikuroi isolates 21 3.2 Microscopic observation of colonization characteristic 22 3.3 Microscopic observation in inoculated rice seedlings 22 3.4 Quantification of F. fujikuroi in inoculated rice seedlings 24 3.5 GA3 sensitivity test 27 3.6 Association between bakanae symptoms and biomass of F. fujikuroi 28 3.7 Macroscopic and microscopic observation in adult field plants 29 3.8 Quantification of F. fujikuroi in adult field plants 30 Chapter 4 Discussion 33 4.1 Pathogenesis of F. fujikuroi in inoculated rice seedlings 33 4.2 Pathogenesis of F. fujikuroi in adult field plants 38 References 44 Table 58 Figures 61 | |
dc.language.iso | en | |
dc.title | 水稻徒長病菌 Fusarium fujikuroi 致病過程探討 | zh_TW |
dc.title | Exploration on the Pathogenesis of Rice Bakanae Disease Caused by Fusarium fujikuroi | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃振文(Jenn-Wen Huang),劉瑞芬(Ruey-Fen Liou),陳啟予(Chi-Yu Chen),林宗俊(Tsung-Chun Lin) | |
dc.subject.keyword | 水稻徒長病,Fusarium fujikuroi,致病過程,螢光顯微觀察,即時定量PCR, | zh_TW |
dc.subject.keyword | Bakanae disease,Fusarium fujikuroi,pathogenesis,fluorescence microscopy,real-time quantitative PCR (qPCR), | en |
dc.relation.page | 84 | |
dc.identifier.doi | 10.6342/NTU201904313 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-11-25 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-108-1.pdf 目前未授權公開取用 | 4.17 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。