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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳彥榮(Edward Chern) | |
dc.contributor.author | Po-Hsiang Chang | en |
dc.contributor.author | 張博翔 | zh_TW |
dc.date.accessioned | 2021-06-17T08:47:47Z | - |
dc.date.available | 2021-02-22 | |
dc.date.copyright | 2021-02-22 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2021-01-20 | |
dc.identifier.citation | [1] F. Pampaloni, E.G. Reynaud, E.H. Stelzer, The third dimension bridges the gap between cell culture and live tissue, Nat Rev Mol Cell Biol 8(10) (2007) 839-45. [2] K. Duval, H. Grover, L.H. Han, Y. Mou, A.F. Pegoraro, J. Fredberg, Z. Chen, Modeling Physiological Events in 2D vs. 3D Cell Culture, Physiology (Bethesda) 32(4) (2017) 266-277. [3] T. Mammoto, D.E. Ingber, Mechanical control of tissue and organ development, Development 137(9) (2010) 1407-20. [4] G.S. Hussey, J.L. Dziki, S.F. Badylak, Extracellular matrix-based materials for regenerative medicine, Nature Reviews Materials 3(7) (2018) 159-173. [5] X. Gao, C. Xu, N. Asada, P.S. Frenette, The hematopoietic stem cell niche: from embryo to adult, Development 145(2) (2018). [6] R. Peerani, B.M. Rao, C. Bauwens, T. Yin, G.A. Wood, A. Nagy, E. Kumacheva, P.W. Zandstra, Niche-mediated control of human embryonic stem cell self-renewal and differentiation, EMBO J 26(22) (2007) 4744-55. [7] M. Fane, A.T. Weeraratna, How the ageing microenvironment influences tumour progression, Nat Rev Cancer 20(2) (2020) 89-106. [8] M. Shri, H. Agrawal, P. Rani, D. Singh, S.K. Onteru, Hanging Drop, A Best Three-Dimensional (3D) Culture Method for Primary Buffalo and Sheep Hepatocytes, Sci Rep 7(1) (2017) 1203. [9] B. Zohar, Y. Blinder, M. Epshtein, A.A. Szklanny, B. Kaplan, N. Korin, D.J. Mooney, S. Levenberg, Multi-flow channel bioreactor enables real-time monitoring of cellular dynamics in 3D engineered tissue, Commun Biol 2 (2019) 158. [10] G.Y. Lee, P.A. Kenny, E.H. Lee, M.J. Bissell, Three-dimensional culture models of normal and malignant breast epithelial cells, Nat Methods 4(4) (2007) 359-65. [11] J. Kim, B.K. Koo, J.A. Knoblich, Human organoids: model systems for human biology and medicine, Nat Rev Mol Cell Biol 21(10) (2020) 571-584. [12] S. Cheng, Y. Jin, N. Wang, F. Cao, W. Zhang, W. Bai, W. Zheng, X. Jiang, Self-Adjusting, Polymeric Multilayered Roll that can Keep the Shapes of the Blood Vessel Scaffolds during Biodegradation, Advanced Materials 29(28) (2017) 1700171. [13] D.D. McKinnon, D.W. Domaille, J.N. Cha, K.S. Anseth, Biophysically defined and cytocompatible covalently adaptable networks as viscoelastic 3D cell culture systems, Adv Mater 26(6) (2014) 865-72. [14] K. Nagase, M. Yamato, H. Kanazawa, T. Okano, Poly(N-isopropylacrylamide)-based thermoresponsive surfaces provide new types of biomedical applications, Biomaterials 153 (2018) 27-48. [15] P. Rastogi, B. Kandasubramanian, Review of alginate-based hydrogel bioprinting for application in tissue engineering, Biofabrication 11(4) (2019) 042001. [16] N. Bhattarai, J. Gunn, M. Zhang, Chitosan-based hydrogels for controlled, localized drug delivery, Adv Drug Deliv Rev 62(1) (2010) 83-99. [17] Z. Wang, N. Sun, M. Liu, Y. Cao, K. Wang, J. Wang, R. Pei, Multifunctional Nanofibers for Specific Purification and Release of CTCs, ACS Sens 2(4) (2017) 547-552. [18] W.Y. Chuang, T.H. Young, C.H. Yao, W.Y. Chiu, Properties of the poly(vinyl alcohol)/chitosan blend and its effect on the culture of fibroblast in vitro, Biomaterials 20(16) (1999) 1479-87. [19] X. Yang, X. Chen, H. Wang, Acceleration of osteogenic differentiation of preosteoblastic cells by chitosan containing nanofibrous scaffolds, Biomacromolecules 10(10) (2009) 2772-8. [20] S.H. Hsu, S.W. Whu, S.C. Hsieh, C.L. Tsai, D.C. Chen, T.S. Tan, Evaluation of chitosan-alginate-hyaluronate complexes modified by an RGD-containing protein as tissue-engineering scaffolds for cartilage regeneration, Artif Organs 28(8) (2004) 693-703. [21] T.C. Tseng, L. Tao, F.Y. Hsieh, Y. Wei, I.M. Chiu, S.H. Hsu, An Injectable, Self-Healing Hydrogel to Repair the Central Nervous System, Adv Mater 27(23) (2015) 3518-24. [22] D. Rajendran, A. Hussain, D. Yip, A. Parekh, A. Shrirao, C.H. Cho, Long-term liver-specific functions of hepatocytes in electrospun chitosan nanofiber scaffolds coated with fibronectin, J Biomed Mater Res A 105(8) (2017) 2119-2128. [23] Y. Liu, S. Wang, R. Zhang, Composite poly(lactic acid)/chitosan nanofibrous scaffolds for cardiac tissue engineering, Int J Biol Macromol 103 (2017) 1130-1137. [24] S.H. Hsu, T.T. Ho, N.C. Huang, C.L. Yao, L.H. Peng, N.T. Dai, Substrate-dependent modulation of 3D spheroid morphology self-assembled in mesenchymal stem cell-endothelial progenitor cell coculture, Biomaterials 35(26) (2014) 7295-307. [25] Y. Yuan, P. Zhang, Y. Yang, X. Wang, X. Gu, The interaction of Schwann cells with chitosan membranes and fibers in vitro, Biomaterials 25(18) (2004) 4273-8. [26] Y.H. Chen, I.J. Wang, T.H. Young, Formation of keratocyte spheroids on chitosan-coated surface can maintain keratocyte phenotypes, Tissue Eng Part A 15(8) (2009) 2001-13. [27] N.C. Cheng, S. Wang, T.H. Young, The influence of spheroid formation of human adipose-derived stem cells on chitosan films on stemness and differentiation capabilities, Biomaterials 33(6) (2012) 1748-58. [28] G.S. Huang, L.G. Dai, B.L. Yen, S.H. Hsu, Spheroid formation of mesenchymal stem cells on chitosan and chitosan-hyaluronan membranes, Biomaterials 32(29) (2011) 6929-45. [29] S.H. Hsu, Y.L. Lin, T.C. Lin, T.C. Tseng, H.T. Lee, Y.C. Liao, I.M. Chiu, Spheroid Formation from Neural Stem Cells on Chitosan Membranes, Journal of Medical and Biological Engineering 32(2) (2012) 85-90. [30] G.S. Huang, T.C. Tseng, N.T. Dai, K.Y. Fu, L.G. Dai, S.H. Hsu, Fast isolation and expansion of multipotent cells from adipose tissue based on chitosan-selected primary culture, Biomaterials 65 (2015) 154-62. [31] S.H. Hsu, G.S. Huang, F. Feng, Isolation of the multipotent MSC subpopulation from human gingival fibroblasts by culturing on chitosan membranes, Biomaterials 33(9) (2012) 2642-55. [32] S.H. Hsu, G.S. Huang, Substrate-dependent Wnt signaling in MSC differentiation within biomaterial-derived 3D spheroids, Biomaterials 34(20) (2013) 4725-38. [33] H.Y. Yeh, B.H. Liu, M. Sieber, S.H. Hsu, Substrate-dependent gene regulation of self-assembled human MSC spheroids on chitosan membranes, BMC Genomics 15 (2014) 10. [34] C.M. Yang, Y.J. Huang, S.H. Hsu, Enhanced Autophagy of Adipose-Derived Stem Cells Grown on Chitosan Substrates, Biores Open Access 4(1) (2015) 89-96. [35] H.Y. Chiu, Y.G. Tsay, S.C. Hung, Involvement of mTOR-autophagy in the selection of primitive mesenchymal stem cells in chitosan film 3-dimensional culture, Sci Rep 7(1) (2017) 10113. [36] X. Wang, G. Wang, L. Liu, D. Zhang, The mechanism of a chitosan-collagen composite film used as biomaterial support for MC3T3-E1 cell differentiation, Sci Rep 6 (2016) 39322. [37] S.C. Wu, C.H. Chen, J.K. Chang, Y.C. Fu, C.K. Wang, E. R., Y.S. Lin, Y.H. Wang, S.Y. Lin, G.J. Wang, M.L. Ho, Hyaluronan initiates chondrogenesis mainly via CD44 in human adipose-derived stem cells, J Appl Physiol (114) (2013) 1610-1618. [38] Y.D. Kim, H.S. Kim, J. Lee, J.K. Choi, E. Han, J.E. Jeong, Y.S. Cho, ESRP1-Induced CD44 v3 Is Important for Controlling Pluripotency in Human Pluripotent Stem Cells, Stem Cells 36(10) (2018) 1525-1534. [39] S.K. Seidlits, Z.Z. Khaing, R.R. Petersen, J.D. Nickels, J.E. Vanscoy, J.B. Shear, C.E. Schmidt, The effects of hyaluronic acid hydrogels with tunable mechanical properties on neural progenitor cell differentiation, Biomaterials 31(14) (2010) 3930-40. [40] S.K. Seidlits, C.T. Drinnan, R.R. Petersen, J.B. Shear, L.J. Suggs, C.E. Schmidt, Fibronectin-hyaluronic acid composite hydrogels for three-dimensional endothelial cell culture, Acta Biomater 7(6) (2011) 2401-9. [41] S. Gerecht, J.A. Burdick, L.S. Ferreira, S.A. Townsend, R. Langer, G. Vunjak-Novakovic, Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cells, Proc Natl Acad Sci U S A 104(27) (2007) 11298-303. [42] A.E. Erickson, S.K. Lan Levengood, J. Sun, F.C. Chang, M. Zhang, Fabrication and Characterization of Chitosan-Hyaluronic Acid Scaffolds with Varying Stiffness for Glioblastoma Cell Culture, Adv Healthc Mater 7(15) (2018) e1800295. [43] K. Wang, F.M. Kievit, A.E. Erickson, J.R. Silber, R.G. Ellenbogen, M. Zhang, Culture on 3D Chitosan-Hyaluronic Acid Scaffolds Enhances Stem Cell Marker Expression and Drug Resistance in Human Glioblastoma Cancer Stem Cells, Adv Healthc Mater 5(24) (2016) 3173-3181. [44] W.J. Chen, C.C. Ho, Y.L. Chang, H.Y. Chen, C.A. Lin, T.Y. Ling, S.L. Yu, S.S. Yuan, Y.J. Chen, C.Y. Lin, S.H. Pan, H.Y. Chou, Y.J. Chen, G.C. Chang, W.C. Chu, Y.M. Lee, J.Y. Lee, P.J. Lee, K.C. Li, H.W. Chen, P.C. Yang, Cancer-associated fibroblasts regulate the plasticity of lung cancer stemness via paracrine signalling, Nat Commun 5 (2014) 3472. [45] S. Wan, E. Zhao, I. Kryczek, L. Vatan, A. Sadovskaya, G. Ludema, D.M. Simeone, W. Zou, T.H. Welling, Tumor-associated macrophages produce interleukin 6 and signal via STAT3 to promote expansion of human hepatocellular carcinoma stem cells, Gastroenterology 147(6) (2014) 1393-404. [46] J.M. Kelm, N.E. Timmins, C.J. Brown, M. Fussenegger, L.K. Nielsen, Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types, Biotechnol Bioeng 83(2) (2003) 173-80. [47] J. Drost, H. Clevers, Organoids in cancer research, Nat Rev Cancer 18(7) (2018) 407-418. [48] E.L. Fong, S.E. Lamhamedi-Cherradi, E. Burdett, V. Ramamoorthy, A.J. Lazar, F.K. Kasper, M.C. Farach-Carson, D. Vishwamitra, E.G. Demicco, B.A. Menegaz, H.M. Amin, A.G. Mikos, J.A. Ludwig, Modeling Ewing sarcoma tumors in vitro with 3D scaffolds, Proc Natl Acad Sci U S A 110(16) (2013) 6500-5. [49] E. Kaemmerer, F.P. Melchels, B.M. Holzapfel, T. Meckel, D.W. Hutmacher, D. Loessner, Gelatine methacrylamide-based hydrogels: an alternative three-dimensional cancer cell culture system, Acta Biomater 10(6) (2014) 2551-62. [50] J.T. Neal, X. Li, J. Zhu, V. Giangarra, C.L. Grzeskowiak, J. Ju, I.H. Liu, S.H. Chiou, A.A. Salahudeen, A.R. Smith, B.C. Deutsch, L. Liao, A.J. Zemek, F. Zhao, K. Karlsson, L.M. Schultz, T.J. Metzner, L.D. Nadauld, Y.Y. Tseng, S. Alkhairy, C. Oh, P. Keskula, D. Mendoza-Villanueva, F.M. De La Vega, P.L. Kunz, J.C. Liao, J.T. Leppert, J.B. Sunwoo, C. Sabatti, J.S. Boehm, W.C. Hahn, G.X.Y. Zheng, M.M. Davis, C.J. Kuo, Organoid Modeling of the Tumor Immune Microenvironment, Cell 175(7) (2018) 1972-1988 e16. [51] S. Ng, W.J. Tan, M.M.X. Pek, M.H. Tan, M. Kurisawa, Mechanically and chemically defined hydrogel matrices for patient-derived colorectal tumor organoid culture, Biomaterials 219 (2019) 119400. [52] H. Clevers, The cancer stem cell: premises, promises and challenges, Nat Med 17(3) (2011) 313-9. [53] L. Yang, P. Shi, G. Zhao, J. Xu, W. Peng, J. Zhang, G. Zhang, X. Wang, Z. Dong, F. Chen, H. Cui, Targeting cancer stem cell pathways for cancer therapy, Signal Transduct Target Ther 5(1) (2020) 8. [54] L. Vermeulen, E.M.F. De Sousa, M. van der Heijden, K. Cameron, J.H. de Jong, T. Borovski, J.B. Tuynman, M. Todaro, C. Merz, H. Rodermond, M.R. Sprick, K. Kemper, D.J. Richel, G. Stassi, J.P. Medema, Wnt activity defines colon cancer stem cells and is regulated by the microenvironment, Nat Cell Biol 12(5) (2010) 468-76. [55] T.S. Gujral, M. Chan, L. Peshkin, P.K. Sorger, M.W. Kirschner, G. MacBeath, A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis, Cell 159(4) (2014) 844-56. [56] L.L. Marotta, V. Almendro, A. Marusyk, M. Shipitsin, J. Schemme, S.R. Walker, N. Bloushtain-Qimron, J.J. Kim, S.A. Choudhury, R. Maruyama, Z. Wu, M. Gonen, L.A. Mulvey, M.O. Bessarabova, S.J. Huh, S.J. Silver, S.Y. Kim, S.Y. Park, H.E. Lee, K.S. Anderson, A.L. Richardson, T. Nikolskaya, Y. Nikolsky, X.S. Liu, D.E. Root, W.C. Hahn, D.A. Frank, K. Polyak, The JAK2/STAT3 signaling pathway is required for growth of CD44(+)CD24(-) stem cell-like breast cancer cells in human tumors, J Clin Invest 121(7) (2011) 2723-35. [57] P.B. Gupta, I. Pastushenko, A. Skibinski, C. Blanpain, C. Kuperwasser, Phenotypic Plasticity: Driver of Cancer Initiation, Progression, and Therapy Resistance, Cell Stem Cell 24(1) (2019) 65-78. [58] S. Su, J. Chen, H. Yao, J. Liu, S. Yu, L. Lao, M. Wang, M. Luo, Y. Xing, F. Chen, D. Huang, J. Zhao, L. Yang, D. Liao, F. Su, M. Li, Q. Liu, E. Song, CD10(+)GPR77(+) Cancer-Associated Fibroblasts Promote Cancer Formation and Chemoresistance by Sustaining Cancer Stemness, Cell 172(4) (2018) 841-856 e16. [59] K.E. Gomez, F. Wu, S.B. Keysar, J.J. Morton, B. Miller, T.S. Chimed, P.N. Le, C. Nieto, F.N. Chowdhury, A. Tyagi, T.R. Lyons, C.D. Young, H. Zhou, H.L. Somerset, X.J. Wang, A. Jimeno, Cancer Cell CD44 Mediates Macrophage/Monocyte-Driven Regulation of Head and Neck Cancer Stem Cells, Cancer Res 80(19) (2020) 4185-4198. [60] W. Rao, S. Zhao, J. Yu, X. Lu, D.L. Zynger, X. He, Enhanced enrichment of prostate cancer stem-like cells with miniaturized 3D culture in liquid core-hydrogel shell microcapsules, Biomaterials 35(27) (2014) 7762-73. [61] C. Chang, H.L. Goel, H. Gao, B. Pursell, L.D. Shultz, D.L. Greiner, S. Ingerpuu, M. Patarroyo, S. Cao, E. Lim, J. Mao, K.K. McKee, P.D. Yurchenco, A.M. Mercurio, A laminin 511 matrix is regulated by TAZ and functions as the ligand for the alpha6Bbeta1 integrin to sustain breast cancer stem cells, Genes Dev 29(1) (2015) 1-6. [62] S. Tan, A. Yamashita, S.J. Gao, M. Kurisawa, Hyaluronic acid hydrogels with defined crosslink density for the efficient enrichment of breast cancer stem cells, Acta Biomater 94 (2019) 320-329. [63] M. Choi, S.J. Yu, Y. Choi, H.R. Lee, E. Lee, E. Lee, Y. Lee, J. Song, J.G. Son, T.G. Lee, J.Y. Kim, S. Kang, J. Baek, D. Lee, S.G. Im, S. Jon, Polymer Thin Film-Induced Tumor Spheroids Acquire Cancer Stem Cell-like Properties, Cancer Res 78(24) (2018) 6890-6902. [64] H.K. Dhiman, A.R. Ray, A.K. Panda, Three-dimensional chitosan scaffold-based MCF-7 cell culture for the determination of the cytotoxicity of tamoxifen, Biomaterials 26(9) (2005) 979-86. [65] J.L. Horning, S.K. Sahoo, S. Vijayaraghavalu, S. Dimitrijevic, J.K. Vasir, T.K. Jain, A.K. Panda, V. Labhasetwar, 3-D tumor model for in vitro evaluation of anticancer drugs, Mol Pharm 5(5) (2008) 849-62. [66] F.M. Kievit, S.J. Florczyk, M.C. Leung, O. Veiseh, J.O. Park, M.L. Disis, M. Zhang, Chitosan-alginate 3D scaffolds as a mimic of the glioma tumor microenvironment, Biomaterials 31(22) (2010) 5903-10. [67] M. Leung, F.M. Kievit, S.J. Florczyk, O. Veiseh, J. Wu, J.O. Park, M. Zhang, Chitosan-alginate scaffold culture system for hepatocellular carcinoma increases malignancy and drug resistance, Pharm Res 27(9) (2010) 1939-48. [68] N. Arya, V. Sardana, M. Saxena, A. Rangarajan, D.S. Katti, Recapitulating tumour microenvironment in chitosan-gelatin three-dimensional scaffolds: an improved in vitro tumour model, J R Soc Interface 9(77) (2012) 3288-302. [69] J.Z. Wang, Y.X. Zhu, H.C. Ma, S.N. Chen, J.Y. Chao, W.D. Ruan, D. Wang, F.G. Du, Y.Z. Meng, Developing multi-cellular tumor spheroid model (MCTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening, Mater Sci Eng C Mater Biol Appl 62 (2016) 215-25. [70] S.J. Florczyk, G. Liu, F.M. Kievit, A.M. Lewis, J.D. Wu, M. Zhang, 3D porous chitosan-alginate scaffolds: a new matrix for studying prostate cancer cell-lymphocyte interactions in vitro, Adv Healthc Mater 1(5) (2012) 590-9. [71] V. Phan-Lai, S.J. Florczyk, F.M. Kievit, K. Wang, E. Gad, M.L. Disis, M. Zhang, Three-dimensional scaffolds to evaluate tumor associated fibroblast-mediated suppression of breast tumor specific T cells, Biomacromolecules 14(5) (2013) 1330-7. [72] H.W. Han, S.H. Hsu, Chitosan-hyaluronan based 3D co-culture platform for studying the crosstalk of lung cancer cells and mesenchymal stem cells, Acta Biomater 42 (2016) 157-67. [73] S.J. Florczyk, K. Wang, S. Jana, D.L. Wood, S.K. Sytsma, J. Sham, F.M. Kievit, M. Zhang, Porous chitosan-hyaluronic acid scaffolds as a mimic of glioblastoma microenvironment ECM, Biomaterials 34(38) (2013) 10143-50. [74] F.M. Kievit, S.J. Florczyk, M.C. Leung, K. Wang, J.D. Wu, J.R. Silber, R.G. Ellenbogen, J.S. Lee, M. Zhang, Proliferation and enrichment of CD133(+) glioblastoma cancer stem cells on 3D chitosan-alginate scaffolds, Biomaterials 35(33) (2014) 9137-43. [75] J. Sims-Mourtada, R.A. Niamat, S. Samuel, C. Eskridge, E.B. Kmiec, Enrichment of breast cancer stem-like cells by growth on electrospun polycaprolactone-chitosan nanofiber scaffolds, Int J Nanomedicine 9 (2014) 995-1003. [76] Y.J. Huang, S.H. Hsu, Acquisition of epithelial-mesenchymal transition and cancer stem-like phenotypes within chitosan-hyaluronan membrane-derived 3D tumor spheroids, Biomaterials 35(38) (2014) 10070-9. [77] Y. Shiba, S. Fernandes, W.Z. Zhu, D. Filice, V. Muskheli, J. Kim, N.J. Palpant, J. Gantz, K.W. Moyes, H. Reinecke, B. Van Biber, T. Dardas, J.L. Mignone, A. Izawa, R. Hanna, M. Viswanathan, J.D. Gold, M.I. Kotlikoff, N. Sarvazyan, M.W. Kay, C.E. Murry, M.A. Laflamme, Human ES-cell-derived cardiomyocytes electrically couple and suppress arrhythmias in injured hearts, Nature 489(7415) (2012) 322-5. [78] W. Yue, Y. Li, T. Zhang, M. Jiang, Y. Qian, M. Zhang, N. Sheng, S. Feng, K. Tang, X. Yu, Y. Shu, C. Yue, N. Jing, ESC-Derived Basal Forebrain Cholinergic Neurons Ameliorate the Cognitive Symptoms Associated with Alzheimer's Disease in Mouse Models, Stem Cell Reports 5(5) (2015) 776-790. [79] T. Zimmermann, F. Remmers, B. Lutz, J. Leschik, ESC-Derived BDNF-Overexpressing Neural Progenitors Differentially Promote Recovery in Huntington's Disease Models by Enhanced Striatal Differentiation, Stem Cell Reports 7(4) (2016) 693-706. [80] K. Takahashi, S. Yamanaka, Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors, Cell 126(4) (2006) 663-76. [81] K. Takahashi, K. Tanabe, M. Ohnuki, M. Narita, T. Ichisaka, K. Tomoda, S. Yamanaka, Induction of pluripotent stem cells from adult human fibroblasts by defined factors, Cell 131(5) (2007) 861-72. [82] J. Yu, M.A. Vodyanik, K. Smuga-Otto, J. Antosiewicz-Bourget, J.L. Frane, S. Tian, J. Nie, G.A. Jonsdottir, V. Ruotti, R. Stewart, Slukvin, II, J.A. Thomson, Induced pluripotent stem cell lines derived from human somatic cells, Science 318(5858) (2007) 1917-20. [83] L. Weinberger, M. Ayyash, N. Novershtern, J.H. Hanna, Dynamic stem cell states: naive to primed pluripotency in rodents and humans, Nat Rev Mol Cell Biol 17(3) (2016) 155-69. [84] E.Z. Jacobs, S. Warrier, P.J. Volders, E. D'Haene, E. Van Lombergen, L. Vantomme, M. Van der Jeught, B. Heindryckx, B. Menten, S. Vergult, CRISPR/Cas9-mediated genome editing in naive human embryonic stem cells, Sci Rep 7(1) (2017) 16650. [85] J. Hanna, A.W. Cheng, K. Saha, J. Kim, C.J. Lengner, F. Soldner, J.P. Cassady, J. Muffat, B.W. Carey, R. Jaenisch, Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs, Proc Natl Acad Sci U S A 107(20) (2010) 9222-7. [86] Y.S. Chan, J. Goke, J.H. Ng, X. Lu, K.A. Gonzales, C.P. Tan, W.Q. Tng, Z.Z. Hong, Y.S. Lim, H.H. Ng, Induction of a human pluripotent state with distinct regulatory circuitry that resembles preimplantation epiblast, Cell Stem Cell 13(6) (2013) 663-75. [87] O. Gafni, L. Weinberger, A.A. Mansour, Y.S. Manor, E. Chomsky, D. Ben-Yosef, Y. Kalma, S. Viukov, I. Maza, A. Zviran, Y. Rais, Z. Shipony, Z. Mukamel, V. Krupalnik, M. Zerbib, S. Geula, I. Caspi, D. Schneir, T. Shwartz, S. Gilad, D. Amann-Zalcenstein, S. Benjamin, I. Amit, A. Tanay, R. Massarwa, N. Novershtern, J.H. Hanna, Derivation of novel human ground state naive pluripotent stem cells, Nature 504(7479) (2013) 282-6. [88] Y. Takashima, G. Guo, R. Loos, J. Nichols, G. Ficz, F. Krueger, D. Oxley, F. Santos, J. Clarke, W. Mansfield, W. Reik, P. Bertone, A. Smith, Resetting transcription factor control circuitry toward ground-state pluripotency in human, Cell 158(6) (2014) 1254-1269. [89] T.W. Theunissen, B.E. Powell, H. Wang, M. Mitalipova, D.A. Faddah, J. Reddy, Z.P. Fan, D. Maetzel, K. Ganz, L. Shi, T. Lungjangwa, S. Imsoonthornruksa, Y. Stelzer, S. Rangarajan, A. D'Alessio, J. Zhang, Q. Gao, M.M. Dawlaty, R.A. Young, N.S. Gray, R. Jaenisch, Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human Pluripotency, Cell Stem Cell 15(4) (2014) 524-526. [90] C.B. Ware, A.M. Nelson, B. Mecham, J. Hesson, W. Zhou, E.C. Jonlin, A.J. Jimenez-Caliani, X. Deng, C. Cavanaugh, S. Cook, P.J. Tesar, J. Okada, L. Margaretha, H. Sperber, M. Choi, C.A. Blau, P.M. Treuting, R.D. Hawkins, V. Cirulli, H. Ruohola-Baker, Derivation of naive human embryonic stem cells, Proc Natl Acad Sci U S A 111(12) (2014) 4484-9. [91] I. Rodriguez-Piza, Y. Richaud-Patin, R. Vassena, F. Gonzalez, M.J. Barrero, A. Veiga, A. Raya, J.C. Izpisua Belmonte, Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions, Stem Cells 28(1) (2010) 36-44. [92] L. Vallier, M. Alexander, R.A. Pedersen, Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells, J Cell Sci 118(Pt 19) (2005) 4495-509. [93] T.E. Ludwig, M.E. Levenstein, J.M. Jones, W.T. Berggren, E.R. Mitchen, J.L. Frane, L.J. Crandall, C.A. Daigh, K.R. Conard, M.S. Piekarczyk, R.A. Llanas, J.A. Thomson, Derivation of human embryonic stem cells in defined conditions, Nat Biotechnol 24(2) (2006) 185-7. [94] G. Chen, D.R. Gulbranson, Z. Hou, J.M. Bolin, V. Ruotti, M.D. Probasco, K. Smuga-Otto, S.E. Howden, N.R. Diol, N.E. Propson, R. Wagner, G.O. Lee, J. Antosiewicz-Bourget, J.M. Teng, J.A. Thomson, Chemically defined conditions for human iPSC derivation and culture, Nat Methods 8(5) (2011) 424-9. [95] S.-y. Yasuda, T. Ikeda, H. Shahsavarani, N. Yoshida, B. Nayer, M. Hino, N. Vartak-Sharma, H. Suemori, K. Hasegawa, Chemically defined and growth-factor-free culture system for the expansion and derivation of human pluripotent stem cells, Nature Biomedical Engineering 2(3) (2018) 173-182. [96] C. Xu, M.S. Inokuma, J. Denham, K. Golds, P. Kundu, J.D. Gold, M.K. Carpenter, Feeder-free growth of undifferentiated human embryonic stem cells, Nat Biotechnol 19(10) (2001) 971-4. [97] M. Nakagawa, Y. Taniguchi, S. Senda, N. Takizawa, T. Ichisaka, K. Asano, A. Morizane, D. Doi, J. Takahashi, M. Nishizawa, Y. Yoshida, T. Toyoda, K. Osafune, K. Sekiguchi, S. Yamanaka, A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells, Sci Rep 4 (2014) 3594. [98] S. Yamasaki, Y. Taguchi, A. Shimamoto, H. Mukasa, H. Tahara, T. Okamoto, Generation of human induced pluripotent stem (Ips) cells in serum- and feeder-free defined culture and TGF-Beta1 regulation of pluripotency, PLoS One 9(1) (2014) e87151. [99] Z. Tong, A. Solanki, A. Hamilos, O. Levy, K. Wen, X. Yin, J.M. Karp, Application of biomaterials to advance induced pluripotent stem cell research and therapy, EMBO J 34(8) (2015) 987-1008. [100] Y. Mei, K. Saha, S.R. Bogatyrev, J. Yang, A.L. Hook, Z.I. Kalcioglu, S.W. Cho, M. Mitalipova, N. Pyzocha, F. Rojas, K.J. Van Vliet, M.C. Davies, M.R. Alexander, R. Langer, R. Jaenisch, D.G. Anderson, Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells, Nat Mater 9(9) (2010) 768-78. [101] L.G. Villa-Diaz, H. Nandivada, J. Ding, N.C. Nogueira-de-Souza, P.H. Krebsbach, K.S. O'Shea, J. Lahann, G.D. Smith, Synthetic polymer coatings for long-term growth of human embryonic stem cells, Nat Biotechnol 28(6) (2010) 581-3. [102] Y. Lei, D.V. Schaffer, A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation, Proc Natl Acad Sci U S A 110(52) (2013) E5039-48. [103] K. Ikeda, S. Nagata, T. Okitsu, S. Takeuchi, Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells, Sci Rep 7(1) (2017) 2850. [104] C. Kropp, D. Massai, R. Zweigerdt, Progress and challenges in large-scale expansion of human pluripotent stem cells, Process Biochemistry 59 (2017) 244-254. [105] B.S. Borys, T. So, J. Colter, T. Dang, E.L. Roberts, T. Revay, L. Larijani, R. Krawetz, I. Lewis, B. Argiropoulos, D.E. Rancourt, S. Jung, Y. Hashimura, B. Lee, M.S. Kallos, Optimized serial expansion of human induced pluripotent stem cells using low-density inoculation to generate clinically relevant quantities in vertical-wheel bioreactors, Stem Cells Transl Med 9(9) (2020) 1036-1052. [106] L. Rohani, B.S. Borys, G. Razian, P. Naghsh, S. Liu, A.A. Johnson, P. Machiraju, H. Holland, I.A. Lewis, R.A. Groves, D. Toms, P.M.K. Gordon, J.W. Li, T. So, T. Dang, M.S. Kallos, D.E. Rancourt, Stirred suspension bioreactors maintain naive pluripotency of human pluripotent stem cells, Commun Biol 3(1) (2020) 492. [107] T.G. Otsuji, J. Bin, A. Yoshimura, M. Tomura, D. Tateyama, I. Minami, Y. Yoshikawa, K. Aiba, J.E. Heuser, T. Nishino, K. Hasegawa, N. Nakatsuji, A 3D sphere culture system containing functional polymers for large-scale human pluripotent stem cell production, Stem Cell Reports 2(5) (2014) 734-45. [108] K.I. Kamei, Y. Koyama, Y. Tokunaga, Y. Mashimo, M. Yoshioka, C. Fockenberg, R. Mosbergen, O. Korn, C. Wells, Y. Chen, Characterization of Phenotypic and Transcriptional Differences in Human Pluripotent Stem Cells under 2D and 3D Culture Conditions, Adv Healthc Mater 5(22) (2016) 2951-2958. [109] H. Lin, Q. Li, Y. Lei, Three-dimensional tissues using human pluripotent stem cell spheroids as biofabrication building blocks, Biofabrication 9(2) (2017) 025007. [110] L. Koch, A. Deiwick, A. Franke, K. Schwanke, A. Haverich, R. Zweigerdt, B. Chichkov, Laser bioprinting of human induced pluripotent stem cells-the effect of printing and biomaterials on cell survival, pluripotency, and differentiation, Biofabrication 10(3) (2018) 035005. [111] Y. Li, X. Jiang, L. Li, Z.N. Chen, G. Gao, R. Yao, W. Sun, 3D printing human induced pluripotent stem cells with novel hydroxypropyl chitin bioink: scalable expansion and uniform aggregation, Biofabrication 10(4) (2018) 044101. [112] A.N. Sohi, H. Naderi-Manesh, M. Soleimani, E.R. Yasaghi, H.K. Manjili, S. Tavaddod, S. Nojehdehi, Synergistic effect of co-immobilized FGF-2 and vitronectin-derived peptide on feeder-free expansion of induced pluripotent stem cells, Mater Sci Eng C Mater Biol Appl 93 (2018) 157-169. [113] Y. Sugawara, K. Hamada, Y. Yamada, J. Kumai, M. Kanagawa, K. Kobayashi, T. Toda, Y. Negishi, F. Katagiri, K. Hozumi, M. Nomizu, Y. Kikkawa, Characterization of dystroglycan binding in adhesion of human induced pluripotent stem cells to laminin-511 E8 fragment, Sci Rep 9(1) (2019) 13037. [114] Z. Li, M. Leung, R. Hopper, R. Ellenbogen, M. Zhang, Feeder-free self-renewal of human embryonic stem cells in 3D porous natural polymer scaffolds, Biomaterials 31(3) (2010) 404-12. [115] W. Zhang, S. Zhao, W. Rao, J. Snyder, J.K. Choi, J. Wang, I.A. Khan, N.B. Saleh, P.J. Mohler, J. Yu, T.J. Hund, C. Tang, X. He, A Novel Core-Shell Microcapsule for Encapsulation and 3D Culture of Embryonic Stem Cells, J Mater Chem B Mater Biol Med 2013(7) (2013) 1002-1009. [116] Q. Gu, E. Tomaskovic-Crook, G.G. Wallace, J.M. Crook, 3D Bioprinting Human Induced Pluripotent Stem Cell Constructs for In Situ Cell Proliferation and Successive Multilineage Differentiation, Adv Healthc Mater 6(17) (2017). [117] C.W. Wong, Y.T. Chen, C.L. Chien, T.Y. Yu, S.P. Rwei, S.H. Hsu, A simple and efficient feeder-free culture system to up-scale iPSCs on polymeric material surface for use in 3D bioprinting, Mater Sci Eng C Mater Biol Appl 82 (2018) 69-79. [118] Y. Nie, K. Zhang, S. Zhang, D. Wang, Z. Han, Y. Che, D. Kong, Q. Zhao, Z. Han, Z.X. He, N. Liu, F. Ma, Z. Li, Nitric oxide releasing hydrogel promotes endothelial differentiation of mouse embryonic stem cells, Acta Biomater 63 (2017) 190-199. [119] M. Leung, A. Cooper, S. Jana, C.T. Tsao, T.A. Petrie, M. Zhang, Nanofiber-based in vitro system for high myogenic differentiation of human embryonic stem cells, Biomacromolecules 14(12) (2013) 4207-16. [120] A. Cooper, M. Leung, M. Zhang, Polymeric fibrous matrices for substrate-mediated human embryonic stem cell lineage differentiation, Macromol Biosci 12(7) (2012) 882-92. [121] B.A. Lindborg, J.H. Brekke, A.L. Vegoe, C.B. Ulrich, K.T. Haider, S. Subramaniam, S.L. Venhuizen, C.R. Eide, P.J. Orchard, W. Chen, Q. Wang, F. Pelaez, C.M. Scott, E. Kokkoli, S.A. Keirstead, J.R. Dutton, J. Tolar, T.D. O'Brien, Rapid Induction of Cerebral Organoids From Human Induced Pluripotent Stem Cells Using a Chemically Defined Hydrogel and Defined Cell Culture Medium, Stem Cells Transl Med 5(7) (2016) 970-9. [122] L.H. Chen, T.C. Sung, H.H. Lee, A. Higuchi, H.C. Su, K.J. Lin, Y.R. Huang, Q.D. Ling, S.S. Kumar, A.A. Alarfaj, M.A. Munusamy, M. Nasu, D.C. Chen, S.T. Hsu, Y. Chang, K.F. Lee, H.C. Wang, A. Umezawa, Xeno-free and feeder-free culture and differentiation of human embryonic stem cells on recombinant vitronectin-grafted hydrogels, Biomater Sci 7(10) (2019) 4345-4362. [123] N. Takayama, S. Nishimura, S. Nakamura, T. Shimizu, R. Ohnishi, H. Endo, T. Yamaguchi, M. Otsu, K. Nishimura, M. Nakanishi, A. Sawaguchi, R. Nagai, K. Takahashi, S. Yamanaka, H. Nakauchi, K. Eto, Transient activation of c-MYC expression is critical for efficient platelet generation from human induced pluripotent stem cells, J Exp Med 207(13) (2010) 2817-30. [124] A.D. Panopoulos, O. Yanes, S. Ruiz, Y.S. Kida, D. Diep, R. Tautenhahn, A. Herrerias, E.M. Batchelder, N. Plongthongkum, M. Lutz, W.T. Berggren, K. Zhang, R.M. Evans, G. Siuzdak, J.C. Izpisua Belmonte, The metabolome of induced pluripotent stem cells reveals metabolic changes occurring in somatic cell reprogramming, Cell Res 22(1) (2012) 168-77. [125] Q. Zhao, C.A. Gregory, R.H. Lee, R.L. Reger, L. Qin, B. Hai, M.S. Park, N. Yoon, B. Clough, E. McNeill, D.J. Prockop, F. Liu, MSCs derived from iPSCs with a modified protocol are tumor-tropic but have much less potential to promote tumors than bone marrow MSCs, Proc Natl Acad Sci U S A 112(2) (2015) 530-5. [126] Y. Hu, G.K. Smyth, ELDA: extreme limiting dilution analysis for comparing depleted and enriched populations in stem cell and other assays, J Immunol Methods 347(1-2) (2009) 70-8. [127] M. Molenaar, M. van de Wetering, M. Oosterwegel, J. Peterson-Maduro, S. Godsave, V. Korinek, J. Roose, O. Destree, H. Clevers, XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos, Cell 86(3) (1996) 391-9. [128] J. Tchieu, B. Zimmer, F. Fattahi, S. Amin, N. Zeltner, S. Chen, L. Studer, A Modular Platform for Differentiation of Human PSCs into All Major Ectodermal Lineages, Cell Stem Cell 21(3) (2017) 399-410 e7. [129] X. Lian, C. Hsiao, G. Wilson, K. Zhu, L.B. Hazeltine, S.M. Azarin, K.K. Raval, J. Zhang, T.J. Kamp, S.P. Palecek, Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling, Proc Natl Acad Sci U S A 109(27) (2012) E1848-57. [130] K. Si-Tayeb, F.K. Noto, M. Nagaoka, J. Li, M.A. Battle, C. Duris, P.E. North, S. Dalton, S.A. Duncan, Highly efficient generation of human hepatocyte-like cells from induced pluripotent stem cells, Hepatology 51(1) (2010) 297-305. [131] A. Subramanian, P. Tamayo, V.K. Mootha, S. Mukherjee, B.L. Ebert, M.A. Gillette, A. Paulovich, S.L. Pomeroy, T.R. Golub, E.S. Lander, J.P. Mesirov, Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles, Proc Natl Acad Sci U S A 102(43) (2005) 15545-50. [132] J. Munoz, D.E. Stange, A.G. Schepers, M. van de Wetering, B.K. Koo, S. Itzkovitz, R. Volckmann, K.S. Kung, J. Koster, S. Radulescu, K. Myant, R. Versteeg, O.J. Sansom, J.H. van Es, N. Barker, A. van Oudenaarden, S. Mohammed, A.J. Heck, H. Clevers, The Lgr5 intestinal stem cell signature: robust expression of proposed quiescent '+4' cell markers, EMBO J 31(14) (2012) 3079-91. [133] B. Li, C.N. Dewey, RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome, BMC Bioinformatics 12 (2011) 323. [134] N. Leng, J.A. Dawson, J.A. Thomson, V. Ruotti, A.I. Rissman, B.M. Smits, J.D. Haag, M.N. Gould, R.M. Stewart, C. Kendziorski, EBSeq: an empirical Bayes hierarchical model for inference in RNA-seq experiments, Bioinformatics 29(8) (2013) 1035-43. [135] N.L. Bray, H. Pimentel, P. Melsted, L. Pachter, Near-optimal probabilistic RNA-seq quantification, Nat Biotechnol 34(5) (2016) 525-7. [136] F.J. Muller, L.C. Laurent, D. Kostka, I. Ulitsky | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74648 | - |
dc.description.abstract | 二維細胞培養常作為細胞生物學與醫學領域的研究模式,但生物體內的各種組織與器官處於複雜的三維網絡中,因此相較於二維細胞培養,三維細胞培養平台能準確地模擬體內真實的狀態。過去研究指出透明質酸與幾丁聚醣複合膜可作為肺癌細胞、胰臟癌細胞與間葉幹細胞的簡易培養平台。本論文則開發以幾丁聚醣為主的三維細胞培養系統,用於培養大腸癌細胞、肝癌細胞與人類誘導多能幹細胞。本論文第一部份,幾丁聚醣薄膜會促進癌細胞的腫瘤進程與幹細胞特性。除此之外,幾丁聚醣可能會活化 CD44 陽性大腸癌中典型的 Wnt/β-catenin-CD44 訊息路徑,也活化 CD44 陰性肝癌中非典型的 Wnt-STAT3 的訊息路徑。第一部分的研究顯示,幾丁聚醣作為培養基材能促進癌細胞的幹細胞特性,以作為未來研究癌症的基礎。基於幾丁聚醣與細胞幹性的交互作用,此薄膜有潛力成為簡便且符合成本效益的誘導多能幹細胞的培養平台。本論文第二部份,人類誘導多能幹細胞於幾丁聚醣薄膜上能長期維持住其增生速率與多能性,甚至相比於作為控制組的玻連蛋白基質,幾丁聚醣能促進類似於原態多能性的表徵。人類誘導多能幹細胞會於幾丁聚醣薄膜上自組裝形成三維球體,這些球體於此薄膜上可直接誘導成具有三維結構的三胚層細胞。第二部分的研究顯示,幾丁聚醣薄膜不僅能促進人類誘導多能幹細胞的類原態多能性特徵,也能作為一個新穎的三維分化模式。總結來說,此簡便以生醫材料為底的系統能作為一個癌細胞與誘導多能幹細胞的簡便三維培養平台,希望能加速再生醫學、疾病模擬與藥物開發的未來發展。 | zh_TW |
dc.description.abstract | Two-dimensional (2D) adherent cell culture is widely used for research model in cell biology and medicine. However, tissues and organs are constructed through cell-cell and cell-extracellular matrix connections with complex three-dimensional (3D) networks in vertebrates. Emerging evidence indicated that 3D cell culture systems could mimic in vivo conditions and provide the accurate biological properties of cells. Previous studies have demonstrated that chitosan membranes crosslinked with hyaluronic acid could be a simple non-adherent 3D cell culture platform to study lung cancer cells, pancreatic cancer cells, and mesenchymal stem cells. In this dissertation, chitosan-based 3D cell culture system would be developed for the culture of colon cancer cells, hepatocellular carcinoma (HCC) cells, and human induced pluripotent stem cells (hiPSCs). In the first part, chitosan membranes promoted tumor progression and stemness properties. Furthermore, chitosan could activate canonical Wnt/β-catenin-CD44 axis signaling in CD44positive colon cancer cells and noncanonical Wnt-STAT3 signaling in CD44negative HCC cells. Chitosan as a simple culture substrate can regulate cancer stemness for further cancer research and drug screening. On the other hand, based on the relation between chitosan and cell stemness, chitosan probably served as a simplified and cost-effective culture system for hiPSCs. In the second part, chitosan membranes sustained the proliferation and pluripotency of hiPSCs in long-term culture. Moreover, using vitronectin as the comparison group, hiPSCs grown on the membranes displayed naïve-like characteristics. On the chitosan membranes, hiPSCs self-assembled into 3D spheroids which could be directly differentiated into lineage-specific cells from the three germ layers with 3D structures. Chitosan membranes not only promoted the naïve pluripotent features of hiPSCs but also provided a novel 3D differentiation platform. Collectively, this convenient biomaterial-based culture system can provide a convenient platform to study cancer cells and hiPSCs and accelerate the development of regenerative medicine and disease modeling. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T08:47:47Z (GMT). No. of bitstreams: 1 U0001-1601202112375100.pdf: 33832722 bytes, checksum: a2ba132340ae0c8865b307531390b0b0 (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | 口試委員會審定書 I 誌謝 II 中文摘要 III Abstract IV List of Abbreviations VI Chapter 1. Introduction 1 1.1 In vitro cell culture system 1 1.1.1 Three-dimensional cell culture system 1 1.1.2 Polymer-based cell culture system 4 1.1.3 Chitosan 6 1.1.4 Hyaluronic acid 10 1.2 Cancer cell culture 12 1.2.1 Tumor microenvironments 12 1.2.2 Cancer stem cells and cancer stemness 14 1.2.3 Chitosan-based cancer cell culture system 17 1.3 Pluripotent stem cell culture 21 1.3.1 Pluripotent stem cells 21 1.3.2 Feeder-based and feeder-free systems for human pluripotent stem cells 23 1.3.3 3D culture systems for human pluripotent stem cells 26 1.3.4 Chitosan-involved materials for pluripotent stem cells 28 Chapter 2. Motivation and Aim 31 Chapter 3. Materials and Methods 33 3.1 Preparation of chitosan and chitosan-hyaluronic acid membranes 33 3.2 Cell line 34 3.2.1 Culturing cancer cell lines 34 3.2.2 Culturing human induced pluripotent stem cell lines 35 3.2.3 Culturing human mesenchymal stem cells 37 3.2.4 Lentivirus knockdown system 37 3.3 Flow cytometry analysis 38 3.3.1 Extracellular protein marker analysis 38 3.3.2 Intracellular protein marker analysis 39 3.3.3 Aldehyde dehydrogenase activity assay 39 3.3.4 Side population assay 40 3.3.5 Quiescent population assay 40 3.4 Cancer phenotype analysis 41 3.4.1 Transwell migration assay 41 3.4.2 Drug resistance assay 41 3.4.3 Sphere forming assay 42 3.5 Luciferase reporter assay 42 3.6 Immunofluorescence staining 43 3.7 hiPSC phenotype analysis 44 3.7.1 Embryoid body formation assay 44 3.7.2 Teratoma formation assay 44 3.8 Differentiation of hiPSCs 45 3.8.1 Trilineage differentiation 45 3.8.2 Neural stem cell differentiation 45 3.8.3 Cardiomyocyte differentiation 46 3.8.4 Hepatocyte differentiation 47 3.9 Bioinformatics 48 3.9.1 Microarray 48 3.9.2 Bulk RNA sequencing 48 3.10 Western blot 50 3.11 Real-time polymerase chain reaction 50 3.12 Statistical analysis 51 Chapter 4. Chitosan promotes cancer progression and stem cell properties in association with Wnt signaling in colon and hepatocellular carcinoma cells 52 4.1 Experimental design 52 4.2 Results 53 4.2.1 Morphologies of colon cancer and HCC cell lines on the membranes 53 4.2.2 Investigation of gene expression profiling on different substrate-harvested cells 53 4.2.3 Chitosan promoted cell motility and drug resistance 54 4.2.4 Chitosan regulated the cancer stemness properties 55 4.2.5 Chitosan increased the quiescent population 57 4.2.6 Characterization of the Wnt signaling pathways in colon cancer and HCC cells 57 4.2.7 The CD44-dependent effect of the colon cancer cells cultured on the membranes 58 4.3 Discussion 59 Chapter 5. Chitosan membranes serve as a novel feeder-free 3D cell culture system for human induced pluripotent stem cells 67 5.1 Experimental design 67 5.2 Results 68 5.2.1 Characterization of culture substrates and hiPSC behaviors 68 5.2.2 Chitosan-based substrates sustained the pluripotency of hiPSCs 69 5.2.3 Examination of gene expression profiling of hiPSC spheroids 71 5.2.4 Chitosan promoted the naïve-like features of hiPSCs 73 5.2.5 Long-term culture of hiPSCs on chitosan membranes 74 5.2.6 Generation of 3D neural stem cell-like spheroids on chitosan membranes 75 5.2.7 Chitosan membranes served as a 3D differentiation platform for cardiomyocytes 76 5.2.8 Differentiation of hiPSC spheroids into hepatocyte-like spheroids on chitosan membranes 77 5.3 Discussion 78 Chapter 6. Conclusion and Perspective 91 Chapter 7. Figures and Tables 93 Figure 1. Scheme of research into cancer stemness and cell-biomaterial interaction via culturing cancer cells on chitosan-based membranes. 93 Figure 2. Morphology of cancer cell lines cultured on different surfaces 95 Figure 3. Microarray analysis of HT29 grown on different substrates 97 Figure 4. Analysis of cell motility and migration-associated gene expression 100 Figure 5. Analysis of drug resistance and associated gene expression 104 Figure 6. Evaluation of the stemness properties in substrate-harvested cancer cells 107 Figure 7. Analysis of expression levels of specific markers in other cancer cell lines cultured on different substrates and HT29 harvested from a 3D culture system 109 Figure 8. Characterization of quiescent population for HT29 and Huh7 111 Figure 9. Evaluation of canonical and noncanonical Wnt signaling pathways in cancer cells 114 Figure 10. Effect of Wnt inhibition by IWP-4 treatment on morphology and stemnesss marker gene expression 117 Figure 11. Effect of knockdown of CD44 receptor on morphology and expression of stemness marker genes 120 Figure 12. Scheme of developing a feeder-free culture system and a 3D differentiation platform for hiPSCs using chitosan membranes. 121 Figure 13. Characterization of material properties of culture substrates 123 Figure 14. Characterization of hiPSC responses cultured on different substrates 125 Figure 15. Evaluation of hiPSC pluripotency harvested from three biomaterials 129 Figure 16. Transcriptome analysis of substrate-harvested hiPSCs using RNA sequencing 132 Figure 17. Comparison of expression of shared, naïve, and primed pluripotency markers in hiPSCs cultured on three substrates 137 Figure 18. Chitosan promoted the pluripotency and the naïve-like features in long-term hiPSCs 140 Figure 19. Karyotype analysis and expression of different pluripotency markers in hiPSCs cultured on VTN substrates and CS membranes 144 Figure 20. Neural differentiation of hiPSC spheroids harvested from CS membranes 146 Figure 21. Generation of cardiomyocyte spheroids from hiPSC spheroids cultured on CS membranes 148 Figure 22. 3D hepatocyte differentiation of CS-harvested hiPSC spheroids 150 Table 1. Antibody information 151 Table 2. RT-PCR primers and shRNA information 153 References 158 | |
dc.language.iso | en | |
dc.title | 以幾丁聚醣薄膜作為癌細胞與人類誘導多能幹細胞之新穎三維細胞培養平台 | zh_TW |
dc.title | Chitosan Membranes as A Novel 3D Cell Culture Platform for Cancer Cells and Human Induced Pluripotent Stem Cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 109-1 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 徐善慧(Shan-hui Hsu),陳佑宗(You-Tzung Chen),黃兆祺(Eric Hwang),侯詠德(Yung-Te Hou),黃楓婷(Feng-Ting Huang) | |
dc.subject.keyword | 幾丁聚醣,三維細胞培養系統,癌症幹性,人類誘導多能幹細胞, | zh_TW |
dc.subject.keyword | chitosan,3D cell culture system,cancer stemness,human induced pluripotent stem cell, | en |
dc.relation.page | 174 | |
dc.identifier.doi | 10.6342/NTU202100071 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2021-01-20 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
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U0001-1601202112375100.pdf 目前未授權公開取用 | 33.04 MB | Adobe PDF |
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