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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林順福 | |
dc.contributor.author | Yi-Ting Lien | en |
dc.contributor.author | 連苡廷 | zh_TW |
dc.date.accessioned | 2021-06-17T08:42:37Z | - |
dc.date.available | 2024-08-22 | |
dc.date.copyright | 2019-08-22 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-07 | |
dc.identifier.citation | 日本水稻品種特性檢索網站。2019年7月1日。品種名檢索。取自http://ineweb.narcc.affrc.go.jp/index.html
日本農林水產省。2019年2月。平成30年産酒造好適米の生産状況。取自http://www.maff.go.jp/j/seisaku_tokatu/kikaku/sake_30seisan.html 行政院農委會農糧署統計室。2018a年6月27日。農業統計年報(106年) - 作物生產。取自https://agrstat.coa.gov.tw/sdweb/public/book/Book.aspx 行政院農委會農糧署統計室。2018b年10月1日。糧食供需年報(106年) - 糧食平衡表。取自https://agrstat.coa.gov.tw/sdweb/public/book/Book.aspx 交通部中央氣象局。2019年7月1日。觀測資料查詢。取自http://e-service.cwb.gov.tw/HistoryDataQuery/ 江幡守衛。1961。心白米に関する研究:第4報 心白の発現に及ぼす夜温の影響。日本作物學會紀事 29(4):409-411。 池上勝、吉田晋弥、中村千春、上島脩志。2003。選抜反応から推定した酒米品種「山田錦」の心白発現の遺伝率。育種学研究 5(1):9-15。 吳永培。2007。國產稻米的加值利用。農業試驗所技術服務 18(3):10-14。 吳岱融、林妤姍、王仁助、劉雲霖、吳添金、邱家玉、許志聖、張素貞。2013。水稻釀酒新品種苗栗1號。苗栗區農業改良場研究彙報 3:1-10。 泉沢直、奥津喜章、石原正敏。1998。水稲新認定品種「美山錦」について。茨城県農業総合センタ-農業研究所研究報告 5:27-32。 習敏、林趙淼、趙豔嶺、張新城、楊小雨、劉正輝、李剛華、王紹華、丁豔鋒。2016。氮肥對稉稻籽粒腹白和心白發生及生化組成的影響。中國水稻科學 2:193-199。 陳正昇、陳榮坤、金漢煊、林彥蓉。2009。以分子輔助選種導入 hd1, Hd6 和 ehd1 抽穗期基因至水稻越光品種。作物、環境與生物資訊 7:1-20。 陳榮坤、林彥蓉、羅正宗。2012。水稻新品種臺南16號之育成。臺南區農業改良場研究彙報 60:1-12。 陳榮坤、蔡孟勳、陳凱儀。2013。臺灣稉型水稻品種隨機型SNP分子標誌資料庫的建構。臺南區農業改良場研究彙報 61:15-28。 楊嘉凌、羅義植。2008。酒米品種心白特性的遺傳。臺中區農業改良場研究彙報 99:37-49。 齊藤博之。1998。酒造好適米の判別ならびに酒造米育種における酒造適性推定について。日本醸造協會誌 93(5):327-333。 稻作研究會ask。2009。酒造好適米の栽培テキスト。 簡祥庭、陳榮坤、侯藹玲、陳正昇、林彥蓉。2011。Hd1, Hd6 與 Ehd1 對水稻抽穗期之影響。作物、環境與生物資訊 8(1):45-57。 霧峰農會酒莊。2019年1月。來說酒話- 層層把關就是要讓你吃到品質最好的—霧峰的益全香米。取自https://www.twwfsake.com/index.php Abe, Y., Mieda, K., Ando, T., Kono, I., Yano, M., Kitano, H., and Iwasaki, Y. 2010. 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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74558 | - |
dc.description.abstract | 由於臺灣常有稻米生產過剩的問題,水稻育種計畫逐漸轉為重視品質及多元用途。因此,開發水稻酒米品種為新的育種目標之一。本研究以臺灣稉稻品種台南16號與日本酒米品種美山錦做為雜交親本,建立F2及F2:3族群,以進行釀酒相關性狀、抽穗期及株高等重要性狀的基因座定位,並且探討其遺傳機制。結果顯示,本研究在兩個期作下共偵測到13個QTL與心白性狀相關,其中第3條染色體頂端之qWCE3-1與qWCS3-1為不同期作下穩定調控心白表現的主效QTL,解釋主要的心白外表型變異。然而,僅在一期作中偵測到第4條染色體上大效應之qWCE4-2,為侷限在此環境下作用之基因。這些主效基因為來自美山錦之等位基因時,可提高心白表現。千粒重則定位到10個相關的QTL,其中兩期作均可偵測到第3條染色體之qGWt3-1及第9條染色體之qGWt9-1表現,但兩者效應方向相反,為不同親本提供增加千粒重表現之等位基因。抽穗日數則在不同期作中偵測到3對相同位點之QTL,效應均為正值。其中以qDTH10-1能解釋最多外表型變異,當此QTL為台南16號的同型結合時,在二期作可以延長約12天的抽穗期。此外,亦偵測到11個QTL與株高相關,以及39個QTL與穀粒型態相關。整體而言,本研究自兩期作偵測之QTL位點有多數區域重疊,且性狀相關性分析中有諸多顯著,代表這些基因可能為主要之QTL且具有多效性。本研究也利用QTL緊密連鎖之4個分子標誌,進行基因型篩選,集結酒米重要性狀之基因及臺灣品種之晚抽穗基因,共選拔出21個具有晚抽穗、大心白及大粒重之品系,期望能育成適合臺灣環境栽培之酒米品種。本研究之QTL定位及遺傳分析結果亦可作為未來酒米品種育種之參考。 | zh_TW |
dc.description.abstract | Since it has been a problem of rice over-production in Taiwan, some rice breeding programs are gradually changed to focus on quality and diverse applications. Thus, developing new sake-brewing rice varieties becomes one of the new breeding goals. The purpose of this study was to identify quantitative trait loci (QTL) associated with traits desirable for sake-brewing, including white core size rate (WCS), white core expression rate (WCE), 1000-grain weight (GWt), days to heading (DTH), and grain shape. In two crop seasons, we analyzed genetic structure on F2 and F2:3 populations derived from the cross between Tainan 16 (TN16), a Taiwanese japonica rice variety, and Miyamanishiki (Mi), a Japanese sake-brewing rice. The result of QTL mapping shows that there are thirteen QTLs associated with WCE and WCS in two crop seasons. qWCE3-1 and qWCS3-1 on the apical of chromosome 3 are the major QTLs regulating white core traits in both crop seasons, and qWCE4-2 on chromosome 4 is the major QTL for WCE especially in the lst crop season. These three major QTLs carrying Mi allele would increase WCE and WCS. Moreover, there are ten QTLs associated with GWt. Two pairs of QTLs, qGWt3-1 on chromosome 3 and qGWt9-1 on chromosome 9, were detected in both crop seasons. However, they had oppositive direction of effect which indicated that alleles from different parents could contribute GWt. Three pairs of QTLs associated with DTH were detected in both crop seasons, and qDTH10-1 on chromosome 10 had major effect on the trait. When homozygous alleles of the qDTH10-1 are transferred from TN16 to a line, DTH would extend for about 12 days. In addition, we also detected 11 QTLs controlling plant height and 39 QTLs controlling grain shape. Overall, many QTLs correlated with different traits were mapped on the overlapping regions, and significant correlation between these traits was found. It reveals that these QTLs may have major and pleiotropic effects. Besides, twenty one F2:3 lines were selected by using four SSR markers linked with QTLs related to sake-brewing. These lines with traits, including late heading date, big white core, and heavy grain weight, are expected to become new rice varieties for producing sake in Taiwan. The results of QTL mapping and genetic analysis would provide useful information for future breeding programs of sake-brewing rice. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T08:42:37Z (GMT). No. of bitstreams: 1 ntu-108-R06621118-1.pdf: 2007287 bytes, checksum: d8b24097fbf1cc55be5185903deea958 (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 口試委員會審定書 i
致謝 ii 中文摘要 iii Abstract iv 目錄 vi 表目錄 ix 圖目錄 x 附圖目錄 xi 前言 1 前人研究 3 一、酒米重要性狀 3 二、穀粒粒型及白堊質相關基因之定位 4 三、影響白堊質表現之栽培環境 7 四、水稻分子標誌輔助選種 8 五、國內酒米品種育種現況及引種瓶頸 9 材料與方法 11 一、試驗材料 11 二、分子標誌分析 13 1.水稻葉片DNA萃取 13 2.多型性分子標誌篩選 14 3.PCR分析及瓊脂膠電泳分析 14 三、外表型評估 15 四、連鎖圖譜之建立 16 五、數量性狀基因座定位分析 16 六、優良基因型品系之推薦 17 結果 18 一、連鎖圖譜之建立 18 二、單一基因座分離比 21 三、親本及F2及F2:3分離族群的性狀表現 23 四、性狀間的相關分析 28 1.心白相關性狀 28 2.粒型相關性狀 28 3.其他重要農藝性狀 29 五、數量性狀基因座定位 32 1.心白相關性狀 32 心白面積比例 (white core size rate, WCS) 32 心白米粒比例(white core expression rate, WCE) 36 2.粒型相關性狀 38 千粒重(1000-grain weight, GWt) 38 粒長(grain length, GL) 40 粒寬(grain width, GWh) 42 糙米長寬比(length-to-width ratio, LWR) 44 粒厚(grain thickness, GT) 46 3.其他重要農藝性狀 48 抽穗日數(days to heading, DTH) 48 株高(plant height, PH) 51 六、優良基因型品系之選拔 53 討論 55 一、連鎖圖譜之建立 55 二、親本及F2及F2:3分離族群的性狀表現 55 三、心白相關性狀之數量性狀基因座定位 56 心白面積比例 (white core size rate, WCS) 56 心白米粒比例(white core expression rate, WCE) 56 四、粒型相關性狀之數量性狀基因座定位 58 千粒重(1000-grain weight, GWt) 58 粒長(grain length, GL) 59 粒寬(grain width, GWh) 60 糙米長寬比(length-to-width ratio, LWR) 60 粒厚(grain thickness, GT) 61 五、抽穗日數之數量性狀基因座定位分析 62 六、QTL群集與分子標誌輔助選拔 63 結論 65 附錄 66 參考資料 70 | |
dc.language.iso | zh-TW | |
dc.title | 清酒用稻米性狀及抽穗期之基因定位 | zh_TW |
dc.title | QTL Mapping of Grain Traits and Heading Date
in Sake-brewing Rice | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 賴明信,吳志文,陳榮坤 | |
dc.subject.keyword | 酒米,數量性狀基因座定位,心白,抽穗期,粒重, | zh_TW |
dc.subject.keyword | sake-brewing rice,QTL mapping,white core,heading date,grain weight, | en |
dc.relation.page | 79 | |
dc.identifier.doi | 10.6342/NTU201902739 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-08-07 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
顯示於系所單位: | 農藝學系 |
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