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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 劉雅雯(Ya-Wen Liu) | |
dc.contributor.author | Mei-Chun Chuang | en |
dc.contributor.author | 莊梅均 | zh_TW |
dc.date.accessioned | 2021-06-17T08:35:12Z | - |
dc.date.available | 2019-08-26 | |
dc.date.copyright | 2019-08-26 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-08 | |
dc.identifier.citation | Abmayr, S.M., and Pavlath, G.K. (2012). Myoblast fusion: lessons from flies and mice. Development (Cambridge, England) 139, 641-656.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74427 | - |
dc.description.abstract | 多細胞核的骨骼肌纖維是由肌原母細胞的分化及相互融合而成。目前研究普遍認為細胞骨架-肌動蛋白是肌原母細胞間融合的主要推動力。然而,肌動蛋白在肌原母細胞內是如何被組織調控;以及肌原母細胞是如何具有融合的能力還不甚清楚。本研究指出:肌原母細胞會利用一個主要成分為肌動蛋白的結構,侵襲體,去推動細胞間的融合。隨著細胞分化,肌原母細胞因關鍵的侵襲體銜接蛋白-Tks5的亞型轉換而具有形成侵襲體的能力。Tks5藉由直接與另一個侵襲體蛋白Dynamin-2結合將其聚集到肌原母細胞融合之處,並調控Dynamin-2纏繞到肌動蛋白上的結構,進而增強了Dynamin-2-肌動蛋白束以及侵襲體的物理硬度。綜合以上結果,我們的發現為肌原母細胞間融合的分子機轉帶來曙光,並解開Tks5與Dynamin-2在侵襲體中調控肌動蛋白絲而促使細胞膜融合的新穎結構性功能。 | zh_TW |
dc.description.abstract | The multi-nucleated skeletal muscle fibers arise from the fusion of differentiated myoblasts. While the actin cytoskeleton is known as the driving force for myoblast fusion, how exactly actin is organized in fusing myoblasts and by what means myoblast become fusion competent are poorly understood. In this study, we report that myoblast utilizes an actin-based structure, invadosome, to propel cell-cell fusion. Upon differentiation, myoblasts acquire the ability to form invadosome through the induction of a critical invadosome scaffold protein, the Tyrosine kinase substrate with 5 SH3 domain (Tks5). Tks5 directly interacts with and recruits another invadosome component protein, Dynamin-2 (Dyn2), to the invadosome and regulates Dyn2 assembly around actin filaments. This regulation strengthens the stiffness of Dynamin-actin bundles in vitro and invadosome in vivo. Together, our findings shed light on the molecular mechanism of myogenic fusion machinery and revealed a novel structural function for Tks5 and Dynamin-2 in organizing actin filaments in the invadosome to drive membrane fusion. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T08:35:12Z (GMT). No. of bitstreams: 1 ntu-108-F03448015-1.pdf: 40681279 bytes, checksum: 584fca46387d2b1241a3d7fc431d30f8 (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 口試委員會審定書 …i
Acknowledgement …ii 中文摘要 …iv Abstract …v Table of Contents …vi Chapter 1-Introduction …1 The theoretical model of membrane fusion mechanism …2 The processes of skeletal myogenesis …3 Myoblast fusion: lessons from Drosophila …5 The organization and function of invadosome …6 Tks5, the critical component in invadosome …7 The functions of a membrane remodeling GTPase, Dynamin-2 …8 Chapter 2-Material and Methods …11 Cell culture, transfection, lentiviral and adenoviral infection …12 Molecular biology …13 Immunofluorescent staining and imaging …13 ECM degradation assay …14 Cell fusion assay …14 Dual-luciferase promoter assay …15 Protein purification and GST pull-down assay …15 F-actin bundle sedimentation assay …16 Transmission electron microscopy …16 GTPase activity assay …17 Atomic force microscopy …17 Statistical analysis …19 Chapter 3-Results …20 Invadosomes form in differentiated myoblasts …21 Invadosomes-equipped myoblast tips are the sites of intercellular fusion …22 Invadosomes are required for myoblast fusion, but not differentiation …23 The isoform switching of Tks5 during myogenesis …23 The regulation of Tks5 isoform switch during myogenesis …25 Tks5 isoform switching is required for myoblast fusion …26 Dyn2 is required for myoblast fusion through its actin-related activity …26 Tks5 directly interacts with Dyn2 …28 Tks5 regulates Dyn2 assemble upon actin filaments …29 The localization of Tks5 upon Dyn2-actin bundle …31 Tks5 affects the biochemical properties of Dyn2 mediated actin bundles …31 Tks5 affects the physical properties of Dyn2 mediated actin bundles …32 Chapter 4-Discussion …34 Induction and isoform switch of Tks5 underpin the fusion competency of differentiated myoblast …35 A structural role of Dyn2 in invadosome …37 The stiffness of invadosome in vivo …39 Invadosome in evolution …39 Chapter 5-Figures …41 Figure 1. Examination of C2C12 myoblast morphology and invadosome component proteins during myogenesis …42 Figure 2. The localization of Tks5 and Dyn2 in undifferentiated myoblasts. …44 Figure 3. The localization of invadosome markers in differentiated myoblast …46 Figure 4. The matrix degradation ability of invadosomes …48 Figure 5. Dyn2 enriches at myoblast fusion site …50 Figure 6. Knockdown of Tks5 impairs invadosome formation …52 Figure 7. Invadosome is required for myoblast fusion …54 Figure 8. The protein ratio of Tks5 changes after differentiation …56 Figure 9. Tks5 undergoes isoform switch during myoblast differentiation …58 Figure 10. ChIP-Seq profile of MyoD and Myogenin …60 Figure 11. The promoter region of Tks5 …62 Figure 12. Tks5 promoter activity is increased during myoblast differentiation …64 Figure 13. Isoform switch of Tks5 is critical for myoblast fusion …66 Figure 14. The effects of different Tks5 isoforms expression in myoblasts …68 Figure 15. The mRNA expression level of Dyn2 during myogenesis …70 Figure 16. Effects of Dyn2 knockdown on myogenesis …72 Figure 17. Dyn2 is directly involved in myoblast fusion …74 Figure 18. The association of Tks5 and Dyn2 in myoblast …76 Figure 19. Tks5 directly interacts with Dyn2 …78 Figure 20. GST-Tks5 affects the actin bundling activity of Dyn2 …80 Figure 21. His-Tks5 affects the actin bundling activity of Dyn2 …82 Figure 22. Tks5 regulates Dyn2 assembly around actin …84 Figure 23. GST-AmphII-SH3 affects the actin bundle activity of Dyn2 …86 Figure 24. The localization of Tks5 upon Dyn2-actin bundles were surrounding along Dyn2 spirals …88 Figure 25. The distribution of endogenous Dyn2 in invadosome …90 Figure 26. Tks5 affects the GTPase activity of Dyn2 upon actin filaments …92 Figure 27. Tks5 do not affect the topology of Dyn2-actin bundles …94 Figure 28. Tks5 affects the stiffness of Dyn2-actin bundles …96 Figure 29. The effect of Dyn2 in the stiffness of invadesomes in vivo …98 Figure 30. Working model for Tks5 in invadosome during myoblast fusion …100 Tables …102 Table 1. List of plasmids used in this study …103 Table 2. List of shRNA used in this study …104 Table 3. Lists of sequences of primers used in this study …105 Table 4. List of antibodies used in this study …106 Reference …108 | |
dc.language.iso | en | |
dc.title | 肌原細胞融合時期Tks5在侵襲體形成中扮演之角色 | zh_TW |
dc.title | The Role of Tks5 in Invadosome Formation during Myoblast Fusion | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 李芳仁(Fang-Jen Lee),張智芬(Zee-Fen Chang),鄧述諄(Shu-Chun Teng),郭津岑(Jean-Cheng Kuo) | |
dc.subject.keyword | 肌肉新生,Dyn2,Tks5亞型轉換,侵襲體,肌動蛋白束硬度, | zh_TW |
dc.subject.keyword | myogenesis,Dynamin-2,Tks5 isoform switching,invadosome,actin bundle rigidity, | en |
dc.relation.page | 118 | |
dc.identifier.doi | 10.6342/NTU201902920 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-08-12 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 分子醫學研究所 | zh_TW |
顯示於系所單位: | 分子醫學研究所 |
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