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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 吳瑞菁 | |
dc.contributor.author | Po-Jen Chen | en |
dc.contributor.author | 陳柏任 | zh_TW |
dc.date.accessioned | 2021-06-17T08:30:12Z | - |
dc.date.available | 2029-12-31 | |
dc.date.copyright | 2019-08-27 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-12 | |
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Peng, H.F., Spermatogenic-specific proteins SMZ-1 and SMZ-2 regulate spermatogenesis in the nematode Caenorhabditis elegans, in Department of Clinical Laboratory Sciences and Medical Biotechnology College of Medicine. 2017, National Taiwan University. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74331 | - |
dc.description.abstract | Centriole duplication and disengagement once per cell cycle is important for maintaining the number of centrosome. The regulation mechanisms are coupled with DNA replication and segregation. However, at the end of male meiosis, four haploid sperm cells are generated with a pair of centrioles for each, implying additional centriole duplication event which was uncoupled with DNA replication. We are interested in when centriole duplication happening during male meiosis.
We built up the assay which combined centrosome number and SAS-4 intensity of each centrosome as the marker for the number of centrioles. Through immunofluorescence staining of male germlines and live imaging of spermatocytes, we are able to calculate changes in centriole numbers during meiotic stages. We also developed an algorithm to automatically segment tiny centrosome signals in low signal-to-noise ratio videos. Based on our results, we found the first meiotic duplication spans through entire meiotic prophase for about twenty hours and the second duplication happened shortly after primary meiotic division for roughly ten minutes. The duplication rates and meiotic stages of two duplication events appeared very different. We thought two meiotic duplication events were both different with mitotic centriole duplication. In the future, we could apply our assay under different genetic background or chemical inhibitors to investigate the difference in the regulation mechanisms with mitosis. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T08:30:12Z (GMT). No. of bitstreams: 1 ntu-108-R06424016-1.pdf: 2357791 bytes, checksum: f1b0be038cf3fe5645e2872e327754ea (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii ABSTRACT iii CONTENTS iv LIST OF FIGURES & TABLES vii Introduction 1 Centriole duplication and disengagement 1 Molecular mechanism of centriole duplication 2 Regulation mechanism of centriole duplication 3 One daughter centriole for one mother centriole 3 Daughter centriole is incapable of duplication without conversion 3 Centriole duplication happens at correct timing in cell cycle. 4 Centriole duplication in male meiosis 4 Meiotic cell cycle in C.elegans 4 Possible hypothesis for male meiosis 5 Challenge in studying centriole duplication 5 Materials and methods 7 Immunofluorescence staining 7 Quantification of SAS-4 immunofluorescence intensity 8 UV/TMP integration 9 Live imaging 9 Photobleaching assay of GFP-SAS-4 10 Algorithm for quantification of live imaging 10 Statistical analysis 11 Results 12 Centriole growth before and after primary meiotic division. 13 Generation of integrated GFP-SAS-4 transgenic worm for studying centrosome dynamics during male meiosis. 15 Centrosome movement is related to meiotic cell cycle. 16 Automatic quantification of tiny particles in the low signal-to-noise ratio images. 17 Detection of partially focused images based on the range of intensity and quantified area 18 Centriole growth takes place after primary meiotic division. 19 Centriole duplication in meiosis is under a different regulation mechanism 21 The contribution of microtubule to the second centriole duplication. 23 Discussion 24 The second duplication happening in the M phase might be different with regulation mechanism in mitosis. 24 First centriole duplication takes place during the whole meiotic prophase. 26 Slow centriole growth rates might be a meiosis-specific regulation. 27 Live imaging of GFP-SAS-4 assay in meiotic division enables more accurate interpretation of the observed phenotypes. 28 Automatic detection of centrosomal signals could be applied for other tiny objects. 29 The C-terminus of ZYG-1 play different roles in male meiosis. 30 Figure 31 Table 45 Reference 46 Appendix 49 | |
dc.language.iso | en | |
dc.title | 利用線蟲為模型探討在雄性減數分裂過程的中心粒的複製 | zh_TW |
dc.title | Investigate the Centriole Duplication in C.elegans Spermatogenesis | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 吳益群,潘俊良,蔡欣祐,蘇剛毅 | |
dc.subject.keyword | 中心粒複製,減數分裂,線蟲, | zh_TW |
dc.subject.keyword | centriole duplication,meiosis,C.elegans, | en |
dc.relation.page | 49 | |
dc.identifier.doi | 10.6342/NTU201902892 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-08-12 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 醫學檢驗暨生物技術學研究所 | zh_TW |
顯示於系所單位: | 醫學檢驗暨生物技術學系 |
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