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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | Kuan-Chen Cheng(Kuan-Chen Cheng) | |
dc.contributor.author | Danley Medouze | en |
dc.contributor.author | 馬丹利 | zh_TW |
dc.date.accessioned | 2021-05-19T17:43:20Z | - |
dc.date.available | 2022-08-22 | |
dc.date.available | 2021-05-19T17:43:20Z | - |
dc.date.copyright | 2018-08-22 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-20 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7423 | - |
dc.description.abstract | As the effects of climate change continues to plague the world as a result of our continual use of one of the chief contributor’s ‘fossil fuels’, an alternative to this source of fuel should be developed. Bioethanol is the ideal substitute as it is made up from the fermentation of organic substrates. In this study, Ulva lactuca was used as a feedstock for bioethanol production using a combination of acidic pretreatment and enzymatic hydrolysis; followed by fermentation strategies to determine the effects of agitation, addition of nitrogen sources, substrate loading, temperature, pH on monocultures using Kluyveromyces marxianus K-21 (K-21) and Pichia stipitis BCRC 21777 (BCRC 21777) and a co-culture. The results of the agitation experiments revealed that static culture produced slightly higher ethanol (7.8±0.4 g/L) than agitations at 50 rpm and 150 rpm (6.10±0.7 g/L and 5.90±0.23 g/L) for K-21 and static culture, 50, 150 rpm for Pichia stipitis was 5.21±0.37 g/L, 4.90±0.19 g/L, and 4.80±0.35 g/L respectively. 10% substrate loading was found to be the optimum for both yeast K-21 and BCRC 21777 with ethanol concentrations of 10.60 ± 0.31 g/L and 6.8±0.2 g/L was obtained at optimized fermentation conditions of 35oC, static culture and a pH of 6. For the result of the co-culture a total amount of 11.5±0.7 g/L bioethanol was produced. The results of this research proposes that Ulva lactuca could be used as a feedstock for bioethanol production. | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-05-19T17:43:20Z (GMT). No. of bitstreams: 1 ntu-107-R05642013-1.pdf: 1879324 bytes, checksum: c3e53f593113c0fd7f2f5606daeb52ef (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | Contents
Acknowledgment i Abstract ii List of Figures vi-vii List of Tables viii Appendix ix Time Table x Curriculum vitae xi 1. Introduction 1 2. Literature Review 3 2.1 Environmental Issues Related to Fossil Fuels 3 2.2 Economic Issues Relating to Fossil Fuels 4 2.3 Bioenergy 5 2.3.1 Biodiesel 6 2.3.2 Biogas 7 2.3.3 Bioethanol 8 2.4 Production of Bioethanol 9 2.4.1 Feedstock for Ethanol Production 10 2.4.2 Utilizing Corn for Bioethanol Production 11 2.4.3 Utilizing Sugarcane as a Feedstock for Bioethanol Production 12 2.4.4 Producer Microorganism of Bioethanol 12 2.4.5 Common Yeast used in bioethanol production 13 2.4.5.1 Pichia stipitis sugar metabolism 13 2.4.5.2 Kluyveromuyces marxianus sugar metabolism 14 2.4.6 Fermentation of Starch 14 2.4.7 Fermentation of raw ethanol with sugar raw materials 15 2.4.8 Fermentation of raw ethanol with cellulose raw material 16 2.4.9 Pretreatment of Lignocellulosic Material 16 2.4.9.1 Dilute acid hydrolysis 17 2.5 Fermentation 18 2.5.1 Separate Hydrolysis and Fermentation 18 2.5.2 Co-Fermentation 19 2.6 Seaweed Overview 20 2.6.1 Seaweed Ecological Issues 22 2.6.2 Use of Seaweed for the Production of Bioethanol 24 2.6.3 Overview Ulva lactuca 25 2.6.4 Bioethanol from Ulva 29 3. Experimental Design 32 3.1 Materials and Methods 33 3.1.1 Chemical reagents 33 3.1.2 Equipment 33 3.1.3 Materials 35 3.1.3.1 Samples 35 3.1.3. 2 Microorganism 35 3.2 Method 35 3.2.1 Preparation of seaweed powder 35 3.2.2 Hydrolysis of Samples 35 3.2.2.1 Hot Acid Hydrolysis 35 3.2.2.2 Enzymatic Hydrolysis 36 3.2.3 Fermentation 36 3.2.3.1 Agitation Strategies of static, 50 rpm and 150 rpm on bioethanol production. 36 3.2.3.2 Addition of Nitrogen and Protein sources to the hydrolysate 36 3.2.3.3 Influences of substrate loading on bioethanol production (10 and 15 %) 36 3.2.3.4 Influence of temperature and pH on bioethanol production 37 3.2.3.5 Influence of co-culture on bioethanol production 37 3.2.3.6 Preservation and activation of fermenting yeast 37 3.3 Analytical Methods 38 3.3.1 Yeast cell count 38 3.3.2 Sugar and ethanol analysis by high-performance liquid chromatography (HPLC) 38 4. Results and Discussion 38 4.1 Composition of Ulva lactuca using HPLC 38 4.2 Fermentation study 40 4.2.1 Yeast fermentation and reducing sugar utilization by yeast 40 4.2.1.1 Agitation Study 40 4.2.1.2 Influence of nitrogen and protein sources 44 4.2.1.3 Loading Study 47 4.2.1.4 Temperature Study 51 4.2.1.5 pH Study 55 4.2.1.6 Co-culture Study 58 5. Conclusion 60 6. References 61-67 | |
dc.language.iso | en | |
dc.title | 評估以石蓴水解物生產生質乙醇 | zh_TW |
dc.title | Evaluation of Bioethanol Production from Ulva lactuca Hydroysate | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | Je-Ruei Liu(Je-Ruei Liu),Yen-Hui Chen(Yen-Hui Chen),Chi-Te Liu(Chi-Te Liu) | |
dc.subject.keyword | Lignocellulosic material,Ulva lactuca,co-fermentation,Kluyveromyces marxianus K-21,Pichia stipitis BCRC 21777, | zh_TW |
dc.relation.page | 74 | |
dc.identifier.doi | 10.6342/NTU201803905 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2018-08-20 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物科技研究所 | zh_TW |
顯示於系所單位: | 生物科技研究所 |
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